Evaluation of a commercial electro-kinetically pumped sheath-flow nanospray interface coupled to an automated capillary zone electrophoresis system
Open accessAbstract
Capillary zone electrophoresis-electrospray ionization-mass spectrometry (CZE-ESI-MS) is attracting renewed attention for proteomic and metabolomic analysis. An important reason for this interest is the maturation and commercialization of interfaces for coupling CZE with ESI-MS. One of these interfaces is an electro-kinetically pumped sheath flow nanospray interface developed by the Dovichi group, in which a very low sheath flow is generated based on electroosmosis within a glass emitter. CMP Scientific has commercialized this interface as the EMASS-II ion source. In this work, we compared the performance of the EMASS-II ion source with our in-house system. The performance of the systems is equivalent. We also coupled the EMASS-II ion source with a PrinCE Next|480 capillary electrophoresis autosampler and an Orbitrap mass spectrometer, and analyzed this system's performance in terms of sensitivity, reproducibility, and separation performance for separation of tryptic digests, intact proteins, and amino acids. The system produced reproducible analysis of BSA digest; the RSDs of peptide intensity and migration time across 24 runs were less than 20 and 6%, respectively. The system produced a linear calibration curve of intensity across a 30-fold range of tryptic digest concentration. The combination of a commercial autosampler and electrospray interface efficiently separated amino acids, peptides, and intact proteins, and only required 5 μL of sample for analysis. Graphical Abstract The commercial and locally constructed versions of the interface provide similar numbers of protein identifications from a Xenopus laevis fertilized egg digest.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855 · see citing works
Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment
Cox, J., Neuhauser, N., Michalski, A., Scheltema, R. A., Olsen, J. V., & Mann, M. (2011) · Journal of Proteome Research · doi:10.1021/pr101065j · Cited by 5,349 · see citing works
Cox, J., Hein, M. Y., Luber, C. A., Paron, I., Nagaraj, N., & Mann, M. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.031591 · Cited by 5,180 · see citing works
Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
Smith, R. D., Barinaga, C. J., & Udseth, H. R. (1988) · Analytical Chemistry · doi:10.1021/ac00169a022 · Cited by 534 · see citing works
Deep Proteomics of the Xenopus laevis Egg using an mRNA-Derived Reference Database
Wühr, M., Freeman, R. M., Presler, M., Horb, M. E., Peshkin, L., Gygi, S. P., & Kirschner, M. W. (2014) · Current Biology · doi:10.1016/j.cub.2014.05.044 · Cited by 257 · see citing works
Wojcik, R., Dada, O. O., Sadilek, M., & Dovichi, N. J. (2010) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.4672 · Cited by 234 · see citing works
Self-citationSun, L., Zhu, G., Zhang, Z., Mou, S., & Dovichi, N. J. (2015) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00100 · Cited by 216 · see citing works
Self-citationUltrasensitive and Fast Bottom‐up Analysis of Femtogram Amounts of Complex Proteome Digests
Sun, L., Zhu, G., Zhao, Y., Yan, X., Mou, S., & Dovichi, N. J. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201308139 · Cited by 212 · see citing works
Self-citationMaxwell, E. J., & Chen, D. D. Y. (2008) · Analytica Chimica Acta · doi:10.1016/j.aca.2008.06.034 · Cited by 197 · see citing works
CE‐MS for metabolomics: Developments and applications in the period 2012–2014
Ramautar, R., Somsen, G. W., & de Jong, G. J. (2014) · Electrophoresis · doi:10.1002/elps.201400388 · Cited by 128 · see citing works
Zhu, G., Sun, L., & Dovic̀hi, N. J. (2015) · Talanta · doi:10.1016/j.talanta.2015.06.003 · Cited by 91 · see citing works
Self-citationMetabolome analysis based on capillary electrophoresis-mass spectrometry
Hirayama, A., Wakayama, M., & Soga, T. (2014) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2014.05.005 · Cited by 91 · see citing works
Sun, L., Hebert, A. S., Yan, X., Zhao, Y., Westphall, M. S., Rush, M. J. P., Zhu, G., Champion, M. M., Coon, J. J., & Dovic̀hi, N. J. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201409075 · Cited by 91 · see citing works
Self-citationIntact mass analysis of monoclonal antibodies by capillary electrophoresis—Mass spectrometry
Han, M., Rock, B. M., Pearson, J. T., & Rock, D. A. (2016) · Journal of Chromatography B · doi:10.1016/j.jchromb.2015.12.045 · Cited by 82 · see citing works
Capillary Electrophoresis to Mass Spectrometry Interface Using a Porous Junction
Whitt, J. T., & Moini, M. (2003) · Analytical Chemistry · doi:10.1021/ac026380j · Cited by 74 · see citing works
Sun, X., Lin, L., Liu, X., Zhang, F., Chi, L., Xia, Q., & Linhardt, R. J. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04405 · Cited by 58 · see citing works
Zhao, Y., Riley, N. M., Sun, L., Hebert, A. S., Yan, X., Westphall, M. S., Rush, M. J. P., Zhu, G., Champion, M. M., Medie, F. M., et al. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b00883 · Cited by 55 · see citing works
Self-citationZhao, Y., Sun, L., Knierman, M. D., & Dovichi, N. J. (2016) · Talanta · doi:10.1016/j.talanta.2015.11.020 · Cited by 49 · see citing works
Self-citationCapillary zone electrophoresis for bottom‐up analysis of complex proteomes
Sun, L., Zhu, G., Yan, X., Zhang, Z., Wojcik, R., Champion, M. M., & Dovichi, N. J. (2016) · PROTEOMICS · doi:10.1002/pmic.201500339 · Cited by 41 · see citing works
Self-citationSchiavone, N. M., Sarver, S. A., Sun, L., Wojcik, R., & Dovichi, N. J. (2015) · Journal of Chromatography B · doi:10.1016/j.jchromb.2015.04.001 · Cited by 39 · see citing works
Self-citationPeuchen, E. H., Sun, L., & Dovic̀hi, N. J. (2016) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-016-9564-2 · Cited by 27 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Dai, J., Lamp, J., Xia, Q., & Zhang, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04608 · Cited by 123 · see citing works
Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research
Gomes, F. P., & Yates, J. R. (2019) · Mass Spectrometry Reviews · doi:10.1002/mas.21599 · Cited by 112 · see citing works
Recent advances of capillary electrophoresis-mass spectrometry instrumentation and methodology
Jiang, Y., He, M.-Y., Zhang, W.-J., Luo, P., Guo, D., Fang, X., & Xu, W. (2017) · Chinese Chemical Letters · doi:10.1016/j.cclet.2017.05.008 · Cited by 30 · see citing works
- Authors
- Peuchen, E. H., Zhu, G., Sun, L., & Dovichi, N. J.
- Affiliations
- University of Notre Dame (US)
- ORCID
- Sun, Liangliang
- Venue
- Analytical and Bioanalytical Chemistry
- Published
- 2016-12-16
- Volume
- 409
- Issue
- 7
- Pages
- 1789-1795
- ISSN
- 1618-2642 (ISSN-L)
- 1618-2650
- PMC
- PMC5303660
- OpenAlex
- W2561108669
- Cited by
- 30
- References
- 21
- Citations by year
- 2023: 2 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 6 · 2018: 4 · 2017: 6
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Analytical and Bioanalytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation (DGE-1313583)
- National Institutes of Health (R01HD084399)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- electrokinetic pumping, capillary zone electrophoresis, electrospray ionization, Orbitrap mass spectrometry, tryptic digest, intact protein separation, amino acid separation, peptide identification
- MeSH terms
- Amino Acids, Automation, Calibration, Kinetics, Peptides, Reproducibility of Results, Electrophoresis, Capillary, Spectrometry, Mass, Electrospray Ionization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
