Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure
Abstract
The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons in the brain suggests that lynx1 may be similarly expressed in the lung. To study this, cDNAs encoding lynx1 were cloned from rhesus monkey lung. The temporal expression of lynx1 was studied in pre- and postnatal monkey lungs by in situ hybridization, immunohistochemistry, and realtime polymerase chain reaction (PCR). Lynx1 mRNA signal and lynx1 immunohistochemical staining were localized predominantly in airway epithelial cells, submucous glands, and smooth muscle cells, in endothelial and smooth muscle cells in vessel walls, and in alveolar type II cells. The distribution of lynx1 was similar to that of alpha4, beta2, and beta4 nAChR expression as determined by immunohistochemistry. Immunohistochemical staining also co-localized choline acetyltransferase, the enzyme that synthesizes acetylcholine, with lynx1 expression. Lynx1 expression was first observed in 71-day fetal lungs and increased with age. Immunohistochemistry, Western analysis, and realtime PCR analysis showed increased lynx1 expression in lungs following prenatal nicotine exposure. Thus, lynx1 is co-expressed with nAChR in the lung. Alteration of lynx1 levels is a potential new mechanism by which nicotine affects lung development.
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In lung, lynx1 is co-expressed with nAChRs.
“In lung, lynx1 is co-expressed with nAChRs (125).”
From Nicotine is a selective pharmacological chaperone of acetylcholine receptor number and stoichiometry. Implications for drug discovery (2009) · cites this paper as [125]
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- Authors
- Sekhon, H. S., Song, P., Jia, Y., Lindstrom, J., & Spindel, E. R.
- Affiliations
- Oregon National Primate Research Center (US)
- University of Pennsylvania (US)
- Corresponding author
- Spindel, Eliot R.
- Venue
- Cell and Tissue Research
- Published
- 2005-03-19
- Volume
- 320
- Issue
- 2
- Pages
- 287-297
- ISSN
- 0302-766X (ISSN-L)
- 1432-0878
- OpenAlex
- W1995051779
- Cited by
- 31
- References
- 37
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 3 · 2012: 3
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell and Tissue Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Oregon National Primate Research Center
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
- Keywords
- Lynx1, nicotinic acetylcholine receptors, prenatal nicotine exposure, fetal lung development, maternal smoking, lung development, airway epithelial cells, alveolar type II cells, choline acetyltransferase, gene expression
- MeSH terms
- Aging, Amino Acid Sequence, Animals, Animals, Newborn, Choline O-Acetyltransferase, Female, Frozen Sections, Immunohistochemistry, Lung, Macaca mulatta, Membrane Glycoproteins, Molecular Sequence Data, Neuropeptides, Nicotine, Pregnancy, Prenatal Exposure Delayed Effects, Receptors, Nicotinic, RNA, Messenger, Blotting, Western, Up-Regulation, Polymerase Chain Reaction, Sequence Homology, Amino Acid, In Situ Hybridization, Gene Expression Regulation, Developmental
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