Cited by 41Parkinson's Disease Mechanisms and TreatmentsNeurological diseases and metabolismNuclear Receptors and Signaling

Dose- and time-dependent α-synuclein aggregation induced by ferric iron in SK-N-SH cells

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Li, Wen-Jing, Jiang, Hong, Song, Ning, Xie, Jun-Xia·2010·Neuroscience Bulletin

Abstract

Objective Intracellular formation of Lewy body (LB) is one of the hallmarks of Parkinson's disease. The main component of LB is aggregated alpha-synuclein, present in the substantia nigra where iron accumulation also occurs. The present study aimed to study the relationship between iron and alpha-synuclein aggregation.Methods SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h. MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detect alpha-synuclein aggregation.Results With the increase of iron concentration, the cell viability decreased significantly. At the concentrations of 5 mmol/L and 10 mmol/L, iron induced alpha-synuclein aggregation more severely than at the concentration of 1 mmol/L. Besides, 48-h treatment-induced aggregation was more severe than that induced by 24-h treatment, at the corresponding iron concentrations.Conclusion Ferric iron can induce alpha-synuclein aggregation, which is toxic to the cells, in a dose- and time-dependent way.

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  • The aggregation of proteins involved in neurodegenerative disorders, such as α synuclein and hyper-phosphorylated tau protein, has been shown in vitro to be triggered by elevated ferric iron concentrations.

    “The aggregation of proteins involved in neurodegenerative disorders (eg, α synuclein70 and hyper-phosphorylated tau protein) have been shown in vitro to be triggered by elevated ferric iron concentrations.71”

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Research works printed but not linked: 2

  1. Shults CW. Lewy bodies. Proc Natl Acad Sci U S A 2006, 103: 1661-1668.
  2. Duda JE, Lee VM, Trojanowski JQ. Neuropathology of synuclein aggregates. J Neurosci Res 2000, 61: 121-127.

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Authors
Li, W.-J., Jiang, H., Song, N., & Xie, J.-X.
Affiliations
Qingdao University (CN)
Venue
Neuroscience Bulletin
Published
2010-06
Volume
26
Issue
3
Pages
205-210
ISSN
  • 1995-8218 (ISSN-L)
  • 1673-7067
OpenAlex
W394889930
Cited by
41
References
39
Citations by year
2026: 1 · 2025: 2 · 2023: 3 · 2022: 1 · 2021: 6 · 2020: 1 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
0.76
Type
journal article
Language
en
Open access
Locations
  • Neuroscience Bulletin (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (30870858, 30930036)
  • Natural Science Fund for Distinguished Young Scholars of Shandong Province
Topics
  • Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
  • Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
Keywords
alpha-synuclein aggregation, iron(III), Parkinson's disease, Lewy bodies, SK-N-SH cells, dose-dependent toxicity, time-dependent toxicity, cell viability, iron accumulation, substantia nigra
MeSH terms
Cell Survival, Dose-Response Relationship, Drug, Ferric Compounds, Humans, Iron, Thiazoles, Time Factors, Cell Line, Tumor, alpha-Synuclein, Benzothiazoles

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