Dose- and time-dependent α-synuclein aggregation induced by ferric iron in SK-N-SH cells
Open accessAbstract
Objective Intracellular formation of Lewy body (LB) is one of the hallmarks of Parkinson's disease. The main component of LB is aggregated alpha-synuclein, present in the substantia nigra where iron accumulation also occurs. The present study aimed to study the relationship between iron and alpha-synuclein aggregation.Methods SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h. MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detect alpha-synuclein aggregation.Results With the increase of iron concentration, the cell viability decreased significantly. At the concentrations of 5 mmol/L and 10 mmol/L, iron induced alpha-synuclein aggregation more severely than at the concentration of 1 mmol/L. Besides, 48-h treatment-induced aggregation was more severe than that induced by 24-h treatment, at the corresponding iron concentrations.Conclusion Ferric iron can induce alpha-synuclein aggregation, which is toxic to the cells, in a dose- and time-dependent way.
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The aggregation of proteins involved in neurodegenerative disorders, such as α synuclein and hyper-phosphorylated tau protein, has been shown in vitro to be triggered by elevated ferric iron concentrations.
“The aggregation of proteins involved in neurodegenerative disorders (eg, α synuclein70 and hyper-phosphorylated tau protein) have been shown in vitro to be triggered by elevated ferric iron concentrations.71”
From The role of iron in brain ageing and neurodegenerative disorders (2014) · cites this paper as [70]
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- Authors
- Li, W.-J., Jiang, H., Song, N., & Xie, J.-X.
- Affiliations
- Qingdao University (CN)
- Venue
- Neuroscience Bulletin
- Published
- 2010-06
- Volume
- 26
- Issue
- 3
- Pages
- 205-210
- ISSN
- 1995-8218 (ISSN-L)
- 1673-7067
- PMC
- PMC5560295
- OpenAlex
- W394889930
- Cited by
- 41
- References
- 39
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 3 · 2022: 1 · 2021: 6 · 2020: 1 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Neuroscience Bulletin (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30870858, 30930036)
- Natural Science Fund for Distinguished Young Scholars of Shandong Province
- Topics
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- alpha-synuclein aggregation, iron(III), Parkinson's disease, Lewy bodies, SK-N-SH cells, dose-dependent toxicity, time-dependent toxicity, cell viability, iron accumulation, substantia nigra
- MeSH terms
- Cell Survival, Dose-Response Relationship, Drug, Ferric Compounds, Humans, Iron, Thiazoles, Time Factors, Cell Line, Tumor, alpha-Synuclein, Benzothiazoles
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