The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Identification of a specific telomere terminal transferase activity in tetrahymena extracts
Open accessAbstract
We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting of the Tetrahymena and yeast telomeric sequences respectively, each functioned as primers for elongation, while (CCCCAA)4 and two nontelomeric sequence DNA oligomers did not. Efficient synthesis of the TTGGGG repeats depended only on addition of micromolar concentrations of oligomer primer, dGTP, and dTTP to the extract. The activity was sensitive to heat and proteinase K treatment. The repeat addition was independent of both endogenous Tetrahymena DNA and the endogenous alpha-type DNA polymerase; and a greater elongation activity was present during macronuclear development, when a large number of telomeres are formed and replicated, than during vegetative cell growth. We propose that the novel telomere terminal transferase is involved in the addition of telomeric repeats necessary for the replication of chromosome ends in eukaryotes.
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“Such an enzymatic activity was soon discovered by Carol Greider and Elizabeth Blackburn and was named telomerase 50”
From The common biology of cancer and ageing (2007) · cites this paper as [50]
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Metadata
- Authors
- Greider, C. W., & Blackburn, E. H.
- Affiliations
- University of California, Berkeley (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1985-12
- Volume
- 43
- Issue
- 2
- Pages
- 405-413
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2122740168
- Cited by
- 3,400
- References
- 36
- Citations by year
- 2026: 45 · 2025: 83 · 2024: 87 · 2023: 110 · 2022: 98 · 2021: 142 · 2020: 112 · 2019: 127 · 2018: 110 · 2017: 126 · 2016: 116 · 2015: 128 · 2014: 139 · 2013: 124 · 2012: 114
- FWCI
- 21.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 26259)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Keywords
- Tetrahymena, macronuclear development, telomere replication, proteinase K sensitivity, heat sensitivity
- MeSH terms
- Animals, Base Sequence, Chromosomes, DNA Nucleotidylexotransferase, DNA Nucleotidyltransferases, DNA Polymerase II, DNA Replication, Models, Genetic, Saccharomyces cerevisiae, Templates, Genetic, Tetrahymena
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