Cited by 834Nerve injury and regenerationDevelopmental Biology and Gene RegulationNuclear Receptors and Signaling

Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in xenopus ovary

Hallböök, Finn, Ibáñez, Carlos F., Persson, Håkan·1991·Neuron

Abstract

Evolutionary conservation of members of the NGF family in vertebrates was studied by DNA sequence analysis of PCR fragments for NGF, BDNF, and NT-3 from human, rat, chicken, viper, Xenopus, salmon, and ray. The results showed that the three factors are highly conserved from fishes to mammals. Phylogenetic trees reflecting the evolution and speciation of the members of the NGF family were constructed. In addition, the gene for a fourth member of the family, neurotrophin-4 (NT-4), was isolated from Xenopus and viper. The NT-4 gene encodes a precursor protein of 236 amino acids, which is processed into a 123 amino acid mature NT-4 protein with 50%-60% amino acid identity to NGF, BDNF, and NT-3. The NT-4 protein was shown to interact with the low affinity NGF receptor and elicited neurite outgrowth from explanted dorsal root ganglia with no and lower activity in sympathetic and nodose ganglia, respectively. Northern blot analysis of different tissues from Xenopus showed NT-4 mRNA only in ovary, where it was present at levels over 100-fold higher than those of NGF mRNA in heart.

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Crossref · 10.1016/0896-6273(91)90180-8

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  • NT-3 and NT-4 were identified by sequence similarity to NGF and BDNF.

    “The first neurotrophic factors, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) were discovered through their ability to support the survival of neurons and neurites during development15–17; other members of the neurotrophin family, neurotrophin-3 (NT-3)18–20 and neurotrophin-4 (NT-4)21–23 were then identified through sequence similarity to NGF and BDNF.”

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Authors
Hallböök, F., Ibáñez, C. F., & Persson, H.
Affiliations
Karolinska Institutet (SE)
Venue
Neuron
Publisher
Elsevier BV
Published
1991-05
Volume
6
Issue
5
Pages
845-858
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2119628780
Cited by
834
References
97
Citations by year
2026: 3 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 7 · 2018: 9 · 2017: 8 · 2016: 8 · 2015: 9 · 2014: 21 · 2013: 11 · 2012: 6
FWCI
41.30
Type
journal article
Language
en
Open access
closed
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (2p50ns009199-26a1)
  • National Institute on Aging (AG04418, P01AG004418)
  • National Institute of Neurological Disorders and Stroke (NS09199)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
Keywords
neurotrophins, neurotrophin-4, nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, low-affinity NGF receptor, neurite outgrowth, dorsal root ganglia, evolutionary conservation, phylogenetic analysis, gene isolation, ligand-receptor interactions
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Chickens, Biological Evolution, Female, Molecular Sequence Data, Nerve Growth Factors, Nerve Tissue Proteins, Ovary, Rats, Inbred Strains, RNA, Messenger, Salmon, Skates, Fish, Snakes, Protein Biosynthesis, Xenopus, Gene Expression, Rats

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