Calorie restriction in overweight males ameliorates obesity-related metabolic alterations and cellular adaptations through anti-aging effects, possibly including AMPK and SIRT1 activation
Abstract
Background Calorie restriction (CR) is accepted as an experimental anti-aging paradigm. Several important signal transduction pathways including AMPK and SIRT1 are implicated in the regulation of physiological processes of CR. However, the mechanisms responsible for adaptations remain unclear in humans.Scope of review Four overweight male participants were enrolled and treated with 25% CR of their baseline energy requirements for 7weeks. Characteristics, including body weight (BW), body mass index (BMI), %fat, visceral fat area (VFA), mean blood pressure (MBP) and VO2 max, as well as metabolic parameters, such as insulin, lipid profiles and inflammatory makers and the expression of phosphorylated AMPK and SIRT1 in peripheral blood mononuclear cells (PBMNCs), were determined at baseline and then after 7weeks. In addition, we assessed the effects of the serum collected from the participants on AMPK and SIRT1 activation and mitochondrial biogenesis in cultured human skeletal muscle cells.Major conclusions After CR, BW, BMI, %fat, VFA and MBP all significantly decreased, while VO2 max increased, compared to those at baseline. The levels of fasting insulin, free fatty acid, and inflammatory makers, such as interleukin-6 and visfatin, were significantly reduced, whereas the expression of phosphorylated AMPK and SIRT1 was significantly increased in PBMNCs collected after CR, compared to those at baseline. The skeletal muscle cells that were cultured in serum collected after CR showed an increase in AMPK and SIRT1 activity as well as mitochondrial biogenesis.General significance CR is beneficial for obesity-related metabolic alterations and induces cellular adaptations against aging, possibly through AMPK and SIRT1 activation via circulating factors.
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Caloric restriction is effective in ameliorating obesity-related metabolic impairments and increasing mitochondrial biogenesis markers, primarily through metabolic pathways involving AMPK and SIRT1.
“Nonetheless, caloric restriction is effective in ameliorating obesity-related metabolic impairments and increasing mitochondrial biogenesis markers, primarily through metabolic pathways involving AMPK and SIRT1 [80].”
From Mitochondrial Adaptation in Skeletal Muscle: Impact of Obesity, Caloric Restriction, and Dietary Compounds (2024) · cites this paper as [80]
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- Authors
- Kitada, M., Kume, S., Takeda-Watanabe, A., Tsuda, S.-I., Kanasaki, K., & Koya, D.
- Affiliations
- Kanazawa Medical University (JP)
- Shiga University of Medical Science (JP)
- Corresponding author
- Koya, Daisuke
- Venue
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Publisher
- Elsevier BV
- Published
- 2013-10
- Volume
- 1830
- Issue
- 10
- Pages
- 4820-4827
- ISSN
- 0304-4165 (ISSN-L)
- OpenAlex
- W2001327293
- Cited by
- 60
- References
- 27
- Citations by year
- 2025: 2 · 2024: 7 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 8 · 2016: 8 · 2015: 10 · 2014: 7 · 2013: 1
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Novo Nordisk Pharma
- Grant-in-Aid for Scientific Research (C) (24591218)
- Grant for Promoted Research (S2012-4)
- Kanazawa Medical University
- Grant for Alumni Research (A) (AR2012-09)
- Collaborative Research (C2012-1)
- Specially Promoted Research (SR2012-06)
- 4th Annual Research Award Grant of Japanese Society of Anti-Aging Medicine
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Physiology › Dietary Effects on Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- caloric restriction, obesity, anti-aging, AMPK, SIRT1, mitochondrial biogenesis, skeletal muscle, peripheral blood mononuclear cells, visceral fat, insulin resistance, inflammation, interleukin-6, visfatin, free fatty acids, VO2max, blood pressure, circulating factors
- MeSH terms
- Adenylate Kinase, Adult, Cells, Cultured, Enzyme Activation, Humans, Male, Metabolic Diseases, Middle Aged, Obesity, Muscle, Skeletal, Caloric Restriction, Overweight, Sirtuin 1, Real-Time Polymerase Chain Reaction
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