Object location memory in mice: pharmacological validation and further evidence of hippocampal CA1 participation
Abstract
Object location task (OLT) has been used as a model of hippocampal-dependent memory. Despite this application, there is neither a consistent pharmacological validation of NMDA receptor modulation nor an evaluation of hippocampal participation in mice. In the OLT, mice were placed in the open field with two identical objects for 3 min and, after a delay of 30, 90, 180 or 360 min, one object was moved to a new location and the time spent exploring the objects in new, (novel) and old (familiar) locations was recorded. Our results showed that the mice were able to discriminate object location when tested either 90 or 180 min after training. Intraperitoneal administration of MK801 (NMDA receptors antagonist) or scopolamine (mACh antagonist) induced amnesic effects. On the other hand, D-cycloserine (NMDA agonist) or tacrine (cholinesterase inhibitor) were able to improve memory in the mice tested. In addition, lidocaine infusion in the hippocampal CA1 region 10 min before training blocked object location memory. In short, this work indicates that OLT is susceptible to modulation of NMDA receptors, cholinergic neurotransmission and it is the first to characterize the participation of the hippocampal CA1 region, in this task.
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Pre-sample lidocaine infusion into the CA1 subarea of the dorsal hippocampus disrupted OPP memory in mice.
“Pre-sample lidocaine infusion into the CA1 subarea of the dorsal hippocampus disrupted OPP memory in mice (Assini et al., 2009).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Assini et al., 2009
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- Authors
- Assini, F. L., Duzzioni, M., & Takahashi, R. N.
- Affiliations
- Universidade Federal de Santa Catarina (BR)
- Corresponding author
- Takahashi, Reinaldo Naoto
- ORCID
- Duzzioni, Marcelo
- Venue
- Behavioural Brain Research
- Publisher
- Elsevier BV
- Published
- 2009-12
- Volume
- 204
- Issue
- 1
- Pages
- 206-211
- ISSN
- 0166-4328 (ISSN-L)
- OpenAlex
- W1967807767
- Cited by
- 161
- References
- 52
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 9 · 2023: 9 · 2022: 16 · 2021: 11 · 2020: 15 · 2019: 10 · 2018: 13 · 2017: 7 · 2016: 12 · 2015: 11 · 2014: 7 · 2013: 14 · 2012: 2
- FWCI
- 1.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Behavioural Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Keywords
- object location memory, hippocampal CA1, NMDA receptor, cholinergic neurotransmission, MK-801, scopolamine, D-cycloserine, tacrine, lidocaine, spatial memory, hippocampal-dependent memory, mice
- MeSH terms
- Animals, Central Nervous System Agents, Cholinesterase Inhibitors, Cycloserine, Hippocampus, Injections, Intraperitoneal, Lidocaine, Locomotion, Male, Memory, Receptors, Muscarinic, Scopolamine, Space Perception, Tacrine, Time Factors, Receptors, N-Methyl-D-Aspartate, Dizocilpine Maleate, Excitatory Amino Acid Antagonists, Muscarinic Antagonists, Mice
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