Effects of gut microbiota on the microRNA and mRNA expression in the hippocampus of mice
Abstract
Backgrounds Gut microbiota is increasingly recognized as an important environmental factor that could influence the brain function and behaviors through the microbiota-gut-brain axis.Method Here, we used the germ-free (GF) mice to explore the effect of gut microbiota on hippocampal microRNA (miRNA) and messenger RNAs (mRNAs) expression.Results Behavioral tests showed that, compared to specific pathogen-free (SPF) mice, the GF mice displayed more center time, center distance and less latency to familiar food. Colonization of the GF mice with gut microbiota from SPF mice did not reverse these behaviors. However, 7 differentially expressed miRNAs and 139 mRNAs were significantly restored. Through microRNA Target Filter analysis, 4 of 7 restored miRNAs had 2232 target mRNAs. Among these target mRNAs, 21 target mRNAs levels were decreased. Further analysis showed that the most significant GO terms were metabolic process (GO: 0008152), binding (GO: 0005488) and cell part (GO: 0044464) for biological process, molecular function and cellular component, respectively, and the most significantly altered pathway was axon guidance (mmu04360).Conclusions These findings indicated that colonization of gut microbiota to adolescent GF mice was not sufficient to reverse the behavioral alterations. Gut microbiota could significantly influence the expression levels of miRNAs and mRNAs in hippocampus. Our results could provide original and valuable data for researchers to further study the microbiota-gut-brain axis.
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Evidence from studies in germ-free rodents shows microbial regulation of miRNA expression in brain regions of importance for neuropsychiatry such as the amygdala, prefrontal cortex, and hippocampus.
“evidence emerge from studies in germ-free rodents of microbial regulation of miRNA expression in brain regions of importance for neuropsychiatry such as the amygdala, prefrontal cortex, and hippocampus (Chen et al., 2017;Hoban et al., 2017;Moloney et al., 2017).”
From Gut reactions: Breaking down xenobiotic–microbiome interactions (2019) · cites this paper as Chen et al., 2017
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- Authors
- Chen, J.-J., Zeng, B.-H., Li, W.-W., Zhou, C.-J., Fan, S.-H., Cheng, K., Zeng, L., Zheng, P., Fang, L., Wei, H., & Xie, P.
- Affiliations
- Chongqing Medical University (CN)
- The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
- Army Medical University (CN)
- Corresponding author
- Wei, Hong
- Xie, Peng
- Venue
- Behavioural Brain Research
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 322
- Issue
- Pt A
- Pages
- 34-41
- ISSN
- 0166-4328 (ISSN-L)
- OpenAlex
- W2577550538
- Cited by
- 111
- References
- 44
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 18 · 2023: 6 · 2022: 14 · 2021: 17 · 2020: 10 · 2019: 11 · 2018: 13 · 2017: 4
- FWCI
- 4.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Behavioural Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Special Project on Natural Chronic Non-infectious Diseases (2016YFC1307200)
- Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJ1702037)
- Natural Science Foundation Project of China (81601208, 31300917, 81401140)
- National Basic Research Program of China (2009CB918300)
- Fund for Outstanding Young Scholars in Chongqing Medical University (CYYQ201502)
- Chongqing Science & Technology Commission (cstc2014jcyjA10102)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- gut microbiota, microbiota-gut-brain axis, hippocampus, microRNA expression, mRNA expression, germ-free mice, microbiota colonization, anxiety-like behavior, axon guidance, gene expression profiling
- MeSH terms
- Gastrointestinal Microbiome, Animals, Anxiety, Exploratory Behavior, Feeding Behavior, Hippocampus, Male, Mice, Inbred BALB C, Motor Activity, Psychological Tests, RNA, Messenger, Specific Pathogen-Free Organisms, Stress, Psychological, MicroRNAs
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