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Cited by 448Gastrointestinal motility and disordersGut microbiota and healthProbiotics and Fermented Foods

Specific probiotic therapy attenuates antibiotic induced visceral hypersensitivity in mice

Open access
Verdú, E F, Bercik, P, Verma-Gandhu, M, Huang, X-X, Blennerhassett, P, Jackson, W, Mao, Y, Wang, L +2 more·2006·Gut
DOI 10.1136/gut.2005.066100PMC PMC1856497
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Abstract

Background and aim: Abdominal pain and discomfort are common symptoms in functional disorders and are attributed to visceral hypersensitivity. These symptoms fluctuate over time but the basis for this is unknown. Here we examine the impact of changes in gut flora and gut inflammatory cell activity on visceral sensitivity. Methods: Visceral sensitivity to colorectal distension (CRD) was assessed at intervals in healthy mice for up to 12 weeks, and in mice before and after administration of dexamethasone or non-absorbable antibiotics with or without supplementation with Lactobacillus paracasei (NCC2461). Tissue was obtained for measurement of myeloperoxidase activity (MPO), histology, microbiota analysis, and substance P (SP) immunolabelling. Results: Visceral hypersensitivity developed over time in healthy mice maintained without sterile precautions. This was accompanied by a small increase in MPO activity. Dexamethasone treatment normalised MPO and CRD responses. Antibiotic treatment perturbed gut flora, increased MPO and SP immunoreactivity in the colon, and produced visceral hypersensitivity. Administration of Lactobacillus paracasei in spent culture medium normalised visceral sensitivity and SP immunolabelling, but not intestinal microbiota counts. Conclusion: Perturbations in gut flora and in inflammatory cell activity alter sensory neurotransmitter content in the colon, and result in altered visceral perception. Changes in gut flora may be a basis for the variability of abdominal symptoms observed in functional gastrointestinal disorders and may be prevented by specific probiotic administration.

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  • Probiotics, particularly in animal models, demonstrate that their secreted products or metabolites can modulate contractility of intestinal smooth muscle and visceral sensitivity.

    “The use of probiotics, particularly in animal models, also demonstrates that their secreted products or metabolites can modulate contractility of intestinal smooth muscle and visceral sensitivity.59–61”

    From Intestinal microbiota in functional bowel disorders: a Rome foundation report (2013) · cites this paper as [59]

  • In mice, perturbation of the microbiota by exposure to antibiotics resulted in increased visceromotor responses to colonic distension.

    “In mice, perturbation of the microbiota by exposure to antibiotics resulted in increased visceromotor responses to colonic distension (182).”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [182]

  • In the antibiotic-perturbation mouse model, treatment with Lactobacillus ameliorated inflammatory indexes in the gut wall and reduced the antibiotic-induced visceromotor response.

    “Interestingly, in this model, treatment of mice with Lactobacillus ameliorated inflammatory indexes in the gut wall and reduced the antibiotic-induced visceromotor response.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [182]

  • Several studies linking the gut microbiota with visceral sensitivity have shown that increments in the Lactobacilli family are associated to visceral analgesic-like states.

    “Moreover, several studies linking the gut microbiota with visceral sensitivity, have shown that increments in the Lactobacilli family (during probiotic treatment or during states of dysbiosis) are associated to visceral analgesic-like states.18,19,21,33”

    From Antibiotic-induced dysbiosis alters host-bacterial interactions and leads to colonic sensory and motor changes in mice (2015) · cites this paper as [18]

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1 of the 47 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. Kamm K, Hoppes S, Breves G, et al. Effect of the probiotic yeast Saccaromyces boulardii on the neurochemistry of myenteric neurons in pig jejunum. Neurogastroenterol Motil 2004;16:53-60.
  2. Verma-Gandhu M, Bercik P, Blennerhassett P, et al. Immunodeficiency and visceral hyperalgesia: A putative mechanisms for abdominal pain in AIDS patients. Gastroenterology 2004;126:A161.

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Authors
Verdú, E. F., Bercik, P., Verma-Gandhu, M., Huang, X.-X., Blennerhassett, P., Jackson, W., Mao, Y., Wang, L., Rochat, F., & Collins, S. M.
Affiliations
  • McMaster University (CA)
  • Nestlé (Switzerland) (CH)
Venue
Gut
Publisher
BMJ
Published
2005-08-16
Volume
55
Issue
2
Pages
182-190
ISSN
  • 0017-5749 (ISSN-L)
  • 1468-3288
DOI
10.1136/gut.2005.066100
PMC
PMC1856497
OpenAlex
W2029071453
Cited by
448
References
47
Citations by year
2026: 2 · 2025: 13 · 2024: 19 · 2023: 20 · 2022: 21 · 2021: 25 · 2020: 32 · 2019: 23 · 2018: 23 · 2017: 25 · 2016: 26 · 2015: 27 · 2014: 27 · 2013: 26 · 2012: 26
FWCI
12.85
Type
journal article
Language
en
Open access
Licence
public-domain
Locations
  • Gut (publishedVersion, public-domain)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • Canadian Association of Gastroenterology
  • Canadian Institutes of Health Research
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
visceral hypersensitivity, probiotics, Lactobacillus paracasei, antibiotic-induced dysbiosis, gut microbiota, colorectal distension, dexamethasone, myeloperoxidase, substance P, gut-brain axis, functional gastrointestinal disorders, abdominal pain, intestinal inflammation
MeSH terms
Animals, Anti-Inflammatory Agents, Anti-Bacterial Agents, Dexamethasone, Electromyography, Female, Hyperalgesia, Intestines, Mice, Inbred BALB C, Peroxidase, Physical Stimulation, Substance P, Abdominal Pain, Culture Media, Conditioned, Probiotics, Irritable Bowel Syndrome, Mice

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