Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging
Abstract
Superparamagnetic iron oxide (SPIO) nanoparticles are potential probes for noninvasive cell tracking, but the design of safe probes coupled with high labeling efficiency is still an important objective for such application. In this study, an efficient SPIO probe has been developed for mesenchymal stem cells (MSCs) labeling and tracking. Different from many other systems involving high molecular polycations, we chose low molecular weight amphiphilic PEI2k to form stable nanocomplexes with SPIO nanoparticles. The probe can hold multiple SPIO nanoparticles with a controlled clustering structure, leading to much higher T(2) relaxivities compared to single SPIO nanoparticles. Labeled MSCs are unaffected in their viability, proliferation, or differentiation capacity. The iron uptake process in MSCs displays a time- and dose-dependent behavior. Transmission electron microscopy reveals that the nanoprobes are internalized into the cytoplasm of MSCs. Subcutaneous injection of the labeled MSCs dispersed in a collagen type I hydrogel showed strong image contrast against unlabeled cells under a clinical 3T magnetic resonance imaging (MRI) scanner up to 19 days post-transplantation. This study provides an important alternative to label MSCs at optimized low dosages with high efficiency, and the probe may be useful to label other biologically important cells for imaging studies.
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- Authors
- Liu, G., Wang, Z., Lu, J., Xia, C., Gao, F., Gong, Q., Song, B., Zhao, X., Shuai, X., Chen, X., Ai, H., & Gu, Z.
- Affiliations
- National Institutes of Health (US)
- Sichuan University (CN)
- Sun Yat-sen University (CN)
- Corresponding author
- Ai, Hua
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 32
- Issue
- 2
- Pages
- 528-537
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1989663987
- Cited by
- 142
- References
- 44
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 5 · 2020: 10 · 2019: 5 · 2018: 11 · 2017: 3 · 2016: 16 · 2015: 18 · 2014: 12 · 2013: 16 · 2012: 14
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Materials Science › Materials Chemistry › Lanthanide and Transition Metal Complexes
- Keywords
- superparamagnetic iron oxide nanoparticles, polyethyleneimine, cell labeling, mesenchymal stem cells, cell tracking, MRI contrast agents, T2 relaxivity, nanoparticle uptake, type I collagen gel, in vivo imaging, iron absorption
- MeSH terms
- Animals, Cell Differentiation, Cell Survival, Cells, Cultured, Ferritins, Magnetic Resonance Imaging, Magnetics, Male, Mice, Inbred BALB C, Molecular Weight, Receptors, Transferrin, Apoptosis, Reverse Transcriptase Polymerase Chain Reaction, Mesenchymal Stem Cell Transplantation, Microscopy, Electron, Transmission, Mice, Ferrosoferric Oxide, Nanoparticles, Cell Tracking, Mesenchymal Stem Cells
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