The DOI matches its Crossref record; 110 of the 111 references it cites were found in a registry or an index.
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels
Open accessAbstract
Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“To this end, considerable efforts were made in developing hydrogel-based inks or printing techniques to enhance 3D stability and shape fidelity, such as printing multimaterial inks with combined nozzle system24, 25, photo-crosslinkable hydrogels17, 26, 27, adding viscosifiers28–30, or printing in sacrificial supporting materials31–33.”
From Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles (2023) · cites this paper as [17]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · cited 10×
“Specifically, the RGD motifs do not contain groups that will react with MA, which ensures the retention of good cell adhesive properties of the GelMA [6, 19, 29].”
BackgroundMethodInfluentialSelf-citation3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
Kolesky, D. B., Truby, R. L., Gladman, A. S., Busbee, T. A., Homan, K. A., & Lewis, J. A. (2014) · Advanced Materials · doi:10.1002/adma.201305506 · Cited by 2,102 · cited 3×
“[64] Kolesky DB, Truby RL, Gladman AS, Busbee TA, H oman KA, Lewis JA.”
BackgroundMethodInfluentialStructural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000) · Biomacromolecules · doi:10.1021/bm990017d · Cited by 1,536 · cited 6×
“Co nveniently, introduction of methacryloyl substituent groups confers to gelatin the property of photocrosslinking with the assistance of a photoinitiator and exposure to light, due to the photopolymerization of the methacryloyl substituents [14].”
BackgroundInfluential25th Anniversary Article: Rational Design and Applications of Hydrogels in Regenerative Medicine
Annabi, N., Tamayol, A., Uquillas, J. A., Akbari, M., Bertassoni, L. E., Cha, C., Camci‐Unal, G., Dokmeci, M. R., Peppas, N. A., & Khademhosseini, A. (2014) · Advanced Materials · doi:10.1002/adma.201303233 · Cited by 1,409 · cited 4×
“Hydrogels provide a versatile platform to include desired combinations of properties for designed applications [1].”
BackgroundMethodInfluentialSelf-citationFairbanks, B. D., Schwartz, M. P., Bowman, C. N., & Anseth, K. S. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.08.055 · Cited by 1,206 · cited 5×
“[33] Fairbanks BD, Schwartz MP, Bowman CN, Anseth K S.”
BackgroundInfluentialBilliet, T., Gevaert, E., De Schryver, T., Cornelissen, M., & Dubruel, P. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.09.078 · Cited by 1,015 · cited 2×
“gelatin [14], or gelatin methacrylamide [15-18] in literature by different authors.”
BackgroundBiochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9)
den Steen, P. E. V., Dubois, B., Nelissen, I., Rudd, P., Dwek, R., & Opdenakker, G. (2002) · Critical reviews in biochemistry and molecular biology · doi:10.1080/10409230290771546 · Cited by 948 · cited 1×
“[20] Van den Steen PE, Dubois B, Nelissen I, Rudd P M, Dwek RA, Opdenakker G.”
BackgroundCarbon-Nanotube-Embedded Hydrogel Sheets for Engineering Cardiac Constructs and Bioactuators
Shin, S. R., Jung, S. M., Zalabany, M., Kim, K., Zorlutuna, P., Kim, S. B., Nikkhah, M., Khabiry, M., Azize, M., Kong, J., et al. (2013) · ACS Nano · doi:10.1021/nn305559j · Cited by 946 · cited 5×
“Hybrid hydrogels based on GelMA and other biopolymers The fabrication and use of GelMA hybrids with other biopolymers has been explored for diverse purposes, including enhancing mechanical pr operties [85, 86], tuning characteristics such as porosity, swelling ratio and degradability [86], or imparting a particular pr…”
BackgroundMethodInfluentialSelf-citationHydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs
Bertassoni, L. E., Cecconi, M., Manoharan, V., Nikkhah, M., Hjortnaes, J., Cristino, A. L., Barabaschi, G., Demarchi, D., Dokmeci, M. R., Yang, Y., et al. (2014) · Lab Chip · doi:10.1039/c4lc00030g · Cited by 944 · cited 5×
“The sacrificial agarose segments could be eas ily removed from the GelMA matrix by simply pulling of the agarose cords, resulting in a bioprinted GelMA scaffold with internal hollow “vessels” [63].”
BackgroundMethodInfluentialSelf-citationPhotocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering
Zhao, X., Lang, Q., Yildirimer, L., Lin, Z. Y., Cui, W., Annabi, N., Ng, K. W., Dokmeci, M. R., Ghaemmaghami, A. M., & Khademhosseini, A. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500005 · Cited by 839 · cited 2×
“[104] Zhao X, Lang Q, Yildirimer L, Lin ZY, Cui W, Annabi N, et al.”
BackgroundSelf-citation
Cited by
Sorted by citations
Items 701–800 of 1,004
Advances in the Surface Functionalization of Nanodiamonds for Biological Applications: A Review
Olia, M. B. A., Donnelly, P. S., Hollenberg, L. C. L., Mulvaney, P. A., & Simpson, D. (2021) · ACS Applied Nano Materials · doi:10.1021/acsanm.1c02698 · Cited by 57
Biomaterials in Valvular Heart Diseases
Taghizadeh, B., Ghavami, L., Derakhshankhah, H., Zangene, E., Razmi, M., Jaymand, M., Zarrintaj, P., Zarghami, N., Jaafari, M. R., Shahri, M. M., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.529244 · Cited by 57
Mitochondria Encapsulation in Hydrogel‐Based Artificial Cells as ATP Producing Subunits
Westensee, I. N., Brodszkij, E., Qian, X., Marcelino, T. F., Lefkimmiatis, K., & Städler, B. (2021) · Small · doi:10.1002/smll.202007959 · Cited by 55
Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction
Sharma, V., Dash, S. K., Govarthanan, K., Gahtori, R., Negi, N., Barani, M., Tomar, R., Chakraborty, S., Mathapati, S., Bishi, D. K., et al. (2021) · Cells · doi:10.3390/cells10102538 · Cited by 57
Strategies for sustained release of heparin: A review
Yang, X., Wang, Q., Zhang, A., Shao, X., Liu, T., Tang, B., & Fang, G. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119793 · Cited by 56
Application and development of 3D bioprinting in cartilage tissue engineering
Li, M., Sun, D., Zhang, J., Wang, Y., Wei, Q., & Wang, Y. (2022) · Biomaterials Science · doi:10.1039/d2bm00709f · Cited by 57
3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration
Gao, J., Li, M., Cheng, J., Liu, X., Liu, Z., Liu, J. F., & Tang, P. (2023) · Journal of Functional Biomaterials · doi:10.3390/jfb14020096 · Cited by 56
“Gelatin ethacrylate (Gel A), as one of the ost co monly used photosensitive hydrogel aterials in bone tissue engineering, has good bioco patibility [34].”
BackgroundSmall Joint Organoids 3D Bioprinting: Construction Strategy and Application
Zhang, Y., Li, G., Wang, J., Zhou, F., Ren, X., & Su, J. (2023) · Small · doi:10.1002/smll.202302506 · Cited by 60
Gu, L., Zhang, J., Li, L., Du, Z., Cai, Q., & Yang, X. (2019) · Biomedical Materials · doi:10.1088/1748-605x/ab1583 · Cited by 56
Biomaterial approaches for cardiovascular tissue engineering
Theus, A. S., Tomov, M. L., Cetnar, A. C., Lima, B., Nish, J., McCoy, K., Mahmoudi, M., & Serpooshan, V. (2019) · Emergent Materials · doi:10.1007/s42247-019-00039-3 · Cited by 58
“For instance, gelatin methacrylate (gelMA) [95, 96], hydroxyethyl methacrylate (HEMA) [97], and poly(ethylene glycol) methacrylate (PEGMA) [98] have been used in cardiovascular applications.”
BackgroundPhotocurable Albumin Methacryloyl Hydrogels as a Versatile Platform for Tissue Engineering
Ferracci, G., Zhu, M., Ibrahim, M. S., Ma, G., Fan, T. F., Lee, B. H., & Cho, N. (2020) · ACS Applied Bio Materials · doi:10.1021/acsabm.9b00984 · Cited by 56
Kim, W.-J., & Kim, G. (2020) · Applied Materials Today · doi:10.1016/j.apmt.2020.100588 · Cited by 56
Xiao, L., Lin, J., Chen, R., Huang, Y., Liu, Y., Bai, J., Ge, G., Shi, X., Chen, Y., Shi, J., et al. (2020) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2020/6797154 · Cited by 56
“GelMAwas fabricated as previously reported [37].”
Advanced 3D-Printing Bioinks for Articular Cartilage Repair
Liang, Q., Ma, Y., Yao, X., & Wei, W. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v8i3.511 · Cited by 55
3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications
Kim, J., Kong, J. S., Han, W., Kim, B. S., & Cho, D. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21207757 · Cited by 54
“On the other hand, to irreversibly crosslink gelatin, gelatin-methacrylate (GelMA) bioinks capable of UV photopolymerization are produced by methacrylating gelatin, making it possible to maintain the printed structure even at a physiological temperature [130,131].”
BackgroundRegenerative Medicine for the Treatment of Ischemic Heart Disease; Status and Future Perspectives
Arjmand, B., Abedi, M., Arabi, M., Alavi-Moghadam, S., Rezaei–Tavirani, M., Hadavandkhani, M., Tayanloo-Beik, A., Kordi, R., Roudsari, P. P., & Larijani, B. A. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.704903 · Cited by 57
Chondrocyte-laden GelMA hydrogel combined with 3D printed PLA scaffolds for auricle regeneration
Tang, P., Song, P., Peng, Z., Zhang, B., Gui, X., Wang, Y., Liao, X., Chen, Z., Zhang, Z., Fan, Y., et al. (2021) · Materials Science and Engineering C · doi:10.1016/j.msec.2021.112423 · Cited by 55
Low Intensity Pulsed Ultrasound for Bone Tissue Engineering
McCarthy, C., & Camci‐Unal, G. (2021) · Micromachines · doi:10.3390/mi12121488 · Cited by 55
“Hydrogels have been successful materials for cell scaffolds because they mimic the natural environment of a cell and provide a three-dimensional scaffold for cell growth [90].”
BackgroundTransporting Hydrogel via Chinese Acupuncture Needles for Lesion Positioning Therapy
Lin, F. H., Wang, Z., Xiang, L., Wu, L., Liu, Y., Xi, X., Deng, L., & Cui, W. (2022) · Advanced Science · doi:10.1002/advs.202200079 · Cited by 55
Rosellini, E., & Cascone, M. G. (2023) · Biomimetics · doi:10.3390/biomimetics8010074 · Cited by 55
“The chemical modification of gelatin using methacrylic anhydride (MA) generally involves less than 5% of the amino acid residues in molar ratio, which implies that most of the functional amino acid motifs (such as adhesive motifs and enzyme-degradable motifs) will not be significantly influenced [32].”
BackgroundWalsh, C. M., Wychowaniec, J. K., Costello, L., Brougham, D. F., & Dooley, D. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300951 · Cited by 56
Xue, Y., Li, J., Jiang, T., Han, Q., Jing, Y., Bai, S., & Yan, X. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202302180 · Cited by 55
Wang, K., He, Q., Yang, M., Qiao, Q., Chen, J., Song, J., Zang, N., Hu, H., Xia, L., Xiang, Y., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.70013 · Cited by 58
A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
Zhou, M., Lee, B. H., & Tan, L. P. (2017) · International Journal of Bioprinting · doi:10.18063/ijb.2017.02.003 · Cited by 57
“Moreover, gelatin can be modified with methacrylamide and a minority of methacrylate groups, resulting in a photo-crosslinkable material–gelatin methacryloyl (GelMA)([23]).”
BackgroundCui, J., Wang, H., Shi, Q., Sun, T., Huang, Q., & Fukuda, T. (2019) · Molecules · doi:10.3390/molecules24091762 · Cited by 57
“Although many studies using GelMA have been published, they focused on fabrication of cellular constructs with simple geometry and planar assembly [24,27,33,34].”
BackgroundRandhawa, A., Dutta, S. D., Ganguly, K., Patel, D. K., Patil, T. V., & Lim, K. (2022) · Macromolecular Bioscience · doi:10.1002/mabi.202200278 · Cited by 55
Syed, M. H., Zahari, M. A. K. M., Khan, M. M. R., Beg, M. D. H., & Abdullah, N. (2022) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2022.104121 · Cited by 57
Current Strategies for Engineered Vascular Grafts and Vascularized Tissue Engineering
Chen, J., Zhang, D., Wu, L., & Zhao, M. (2023) · Polymers · doi:10.3390/polym15092015 · Cited by 46
“and spread, while the metalloproteinase-responsive peptide motifs lead to dilation and a decrease in mechanical strength after in vivo implantation [136].”
BackgroundHu, X., Wu, H., Yong, X., Wang, Y., Yang, S., Fan, D., Xiao, Y., Che, L., Shi, K., Li, K., et al. (2023) · MedComm · doi:10.1002/mco2.425 · Cited by 65
Li, P., Dou, X., Feng, C., & Schönherr, H. (2017) · Biomaterials Science · doi:10.1039/c7bm00991g · Cited by 54
Oktay, S., & Alemdar, N. (2018) · Journal of Applied Polymer Science · doi:10.1002/app.46914 · Cited by 54
Bottom-up biofabrication using microfluidic techniques
Nie, M., & Takeuchi, S. (2018) · Biofabrication · doi:10.1088/1758-5090/aadef9 · Cited by 55
Dual Crosslinked Gelatin Methacryloyl Hydrogels for Photolithography and 3D Printing
Basara, G., Yue, X. S., & Zorlutuna, P. (2019) · Gels · doi:10.3390/gels5030034 · Cited by 57
“Moreover, photopatterning can be used to fabricate micropatterned GelMA scaffolds [9,11,12].”
BackgroundHigh-strength hydrogel-based bioinks
Gao, F., Ruan, C., & Liu, W. (2019) · Materials Chemistry Frontiers · doi:10.1039/c9qm00373h · Cited by 55
Bioprinting of novel 3D tumor array chip for drug screening
Xie, M., Gao, Q., Fu, J., Chen, Z., & He, Y. (2020) · Bio-Design and Manufacturing · doi:10.1007/s42242-020-00078-4 · Cited by 56
Advanced Bioink for 3D Bioprinting of Complex Free-Standing Structures with High Stiffness
Gu, Y., Schwarz, B., Forget, A., Barbero, A., Martín, I., & Shastri, V. P. (2020) · Bioengineering · doi:10.3390/bioengineering7040141 · Cited by 54
“Therefore, most reported 3D bioprinting of complex structures require either fugitive phases, like poly(ε-caprolactone), carbohydrate glass, alginate, or Pluronic F127 [11–16], or post-processing like photo-crosslinking [17,18] or chemical crosslinking [19–21], which makes the fabrication complicated and highly demand…”
BackgroundGelatin-based composite hydrogels with biomimetic lubrication and sustained drug release
Zhang, K., Yang, J., Sun, Y., Wang, Y., Liang, J., Luo, J., Cui, W., Deng, L., Xu, X., Wang, B., et al. (2021) · Friction · doi:10.1007/s40544-020-0437-5 · Cited by 53
Lee, H., Kim, W.-J., Lee, J., Park, K.-S., Yoo, J. J., Atala, A., Kim, G., & Lee, S. J. (2021) · Applied Physics Reviews · doi:10.1063/5.0039639 · Cited by 54
Biopolymer-based self-healing hydrogels: A short review
Wang, X., Zhang, H. J., Yang, Y., Chen, Y. M., Zhu, X., & You, X. (2023) · Giant · doi:10.1016/j.giant.2023.100188 · Cited by 53
Semicrystalline Hydrophobically Associated Hydrogels with Integrated High Performances
Wei, D., Yang, J., Zhu, L., Chen, F., Tang, Z., Qin, G., & Chen, Q. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b15843 · Cited by 53
Liu, J., Chuah, Y. J., Fu, J., Zhu, W., & Wang, D. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.04.089 · Cited by 55
Gupta, D., Santoso, J. W., & McCain, M. L. (2021) · Bioengineering · doi:10.3390/bioengineering8010006 · Cited by 53
“Furthermore, gelatin hydrogels have many applications beyond cell culture scaffolds, such as serving as support structures for bioprinted tissues and microfluidic devices or encapsulating drugs for controlled drug release [37], for which they may be incubated in PBS or other buffer solutions.”
BackgroundLee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017) · NPG Asia Materials · doi:10.1038/am.2017.126 · Cited by 52
Zhao, Z. Q., Zhang, B. L., Chu, H. Q., Liang, L., Chen, B. Z., Zheng, H., & Guo, X. D. (2021) · Biomaterials Advances · doi:10.1016/j.msec.2021.112620 · Cited by 52
Kim, D., Kim, M., Lee, J., & Jang, J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.764682 · Cited by 53
Yang, F., Guo, G., & Wang, Y. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121761 · Cited by 52
Lu, B., Ye, M., Xia, J., Zhang, Z., Xiong, Z., & Zhang, T. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300607 · Cited by 52
Sun, J., Li, G., Wu, S., Zou, Y., Weng, W., Gai, T., Chen, X., Zhang, K., Zhou, F., Wang, X., et al. (2023) · Composites Part B Engineering · doi:10.1016/j.compositesb.2023.110803 · Cited by 53
Carriero, V. C., Muzio, L. D., Petralito, S., Casadei, M. A., & Paolicelli, P. (2023) · Gels · doi:10.3390/gels9120979 · Cited by 52
“…APS/TEMED radical cross-linking [77] poly(lactic-co-glycolic acid) HA EDC/NHS [66] HA EDC/NHS [103] heparin EDC/NHS [48] UV photocuring [39] BMP-2 biomimetic peptide and VEGF APS/TEMED [37] hyaluronic GA [58] gelatin EDC/NHS [49] Nano cellulose micro-structuring silica [98] 4.6.”
BackgroundInfluentialTransdermal Drug Delivery System: Current Status and Clinical Application of Microneedles
Xue, Y., Chen, C., Mi, X., Tan, R., Wang, J., Wu, S., Wang, Y., Xiong, R., Chen, M., Tan, W., et al. (2024) · ACS Materials Letters · doi:10.1021/acsmaterialslett.3c01317 · Cited by 56
Chen, S., Xiong, Y., Yang, F., Hu, Y., Feng, J., Zhou, F., Liu, Z., Liu, H., Liu, X., Zhao, J., et al. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.105258 · Cited by 55
Huang, Y., Song, M., Li, X., Du, Y., Gao, Z., Zhao, Y.-Q., Li, C., Yan, H., Mo, X., Wang, C., et al. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101214 · Cited by 55
“The GelMa hydrogel was chosen because of its good biocompatibility and tunable physical properties [35].”
MethodGreene, T., Lin, T., ANDRISANI, O. M., & Lin, C. (2016) · Journal of Applied Polymer Science · doi:10.1002/app.44585 · Cited by 52
“Gelatin can be easily modified with different polymerizable motifs, such as methacrylamide,[1, 2] thiol,[3] and norbornene.”
BackgroundCelikkin, N., Padial, J. S., Costantini, M., Hendrikse, H. C., Cohn, R., Wilson, C. J., Rowan, A. E., & Święszkowski, W. (2018) · Polymers · doi:10.3390/polym10050555 · Cited by 52
A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair
Li, H., Yin, Y., Xiang, Y., Liu, H., & Guo, R. (2020) · Biomedical Materials · doi:10.1088/1748-605x/ab797a · Cited by 52
Shaping collagen for engineering hard tissues: Towards a printomics approach
Maher, M., Castilho, M. D., Yue, Z., Glattauer, V., Hughes, T. C., Ramshaw, J. A. M., & Wallace, G. G. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.06.035 · Cited by 52
Hou, X., Chen, Y., Chen, F., Liu, J., Wang, T., Luo, Y., Jia, S., Wang, P., Tan, S., Lu, B., et al. (2021) · Composites Part B Engineering · doi:10.1016/j.compositesb.2021.109396 · Cited by 52
Qin, X., Wu, Y., Liu, S., Yang, L., Yuan, H., Cai, S., Flesch, J., Li, Z., Tang, Y., Li, X., et al. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.802311 · Cited by 51
“To protect the growth factors from environmental degradation and maintain their biological activity over a long time, hydrogel encapsulation has been used (Holland et al., 2005; Lee and Shin, 2007; Park et al., 2007; Morrell et al., 2008; Wan et al., 2008; Shachar et al., 2011; Yue et al., 2015).”
Background3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
Duan, J., Cao, Y., Shen, Z., Cheng, Y., Ma, Z., Wang, L., Zhang, Y., An, Y., & Sang, S. (2022) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2111.11003 · Cited by 51
“Currently, hydrogels are considered suitable materials for the biological field, while gelatin is composed of a matrix metalloproteinase (MMP) target pattern formed by hydrolysis of mammalian collagen [17].”
BackgroundWang, Y., Song, P., Wu, L., Su, Z., Gui, X., Gao, C., Zhao, H., Wang, Y., Li, Z., Cen, Y., et al. (2022) · Journal of Materials Chemistry B · doi:10.1039/d2tb02371g · Cited by 51
Soft, strong, tough, and durable protein-based fiber hydrogels
Wang, M., Sun, S., Dong, G., Long, F., & Butcher, J. T. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2213030120 · Cited by 51
“Gelatin-based hydrogels, especially gelatin methacryloyl (GelMA) hydrogels, have gained a widespread popularity for various biomedical applications (27, 28).”
Cheng, Q., & Hsu, S. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.04.023 · Cited by 53
Bacterial Cellulose-Based Materials: A Perspective on Cardiovascular Tissue Engineering Applications
Fooladi, S., Nematollahi, M. H., Rabiee, N., & Iravani, S. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.3c00300 · Cited by 51
Graphene: A Multifaceted Carbon-Based Material for Bone Tissue Engineering Applications
Govindarajan, D., Sekaran, S., Swathi, S., & Vimalraj, S. (2023) · ACS Omega · doi:10.1021/acsomega.3c07062 · Cited by 51
“11 Biomedical applications for graphene and its derivatives are promising due to their mechanical, electrical, and optical properties.”
BackgroundInstantly adhesive and ultra-elastic patches for dynamic organ and wound repair
Chansoria, P., Chaudhari, A. P., Etter, E. L., Bonacquisti, E. E., Heavey, M. K., Le, J., Maruthamuthu, M. K., Kussatz, C. C., Blackwell, J., Jasiewicz, N. E., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-48980-0 · Cited by 58
“Conventional GelMA does not show any tissue adhesiveness, only cell adhesion which is intrinsic to all GelMA-based matrices due to the presence of RGD moieties 22 .”
Qu, Y., Zeng, H., Wang, L., Ge, Z., Liu, B., & Fan, Z. (2025) · Advanced Functional Materials · doi:10.1002/adfm.202418076 · Cited by 52
Padhi, J. R., Nayak, D., Nanda, A., Rauta, P. R., Ashe, S., & Nayak, B. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.07.098 · Cited by 51
Singh, S., Awuah, D., Rostam, H. M., Emes, R. D., Kandola, N. K., Onion, D., Htwe, S. S., Rajchagool, B., Cha, B.-H., Kim, D. J., et al. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.7b00104 · Cited by 51
Self-citationDarvishi, S., Souissi, M., Kharaziha, M., Karimzadeh, F., Sahara, R., & Ahadian, S. (2017) · Electrochimica Acta · doi:10.1016/j.electacta.2017.12.126 · Cited by 51
Sun, A. X., Prest, T. A., Fowler, J. R., Brick, R. M., Gloss, K. M., Li, X., Dehart, M., Shen, H., Yang, G., Brown, B. N., et al. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.01.038 · Cited by 55
Tigner, T. J., Rajput, S., Gaharwar, A. K., & Alge, D. L. (2019) · Biomacromolecules · doi:10.1021/acs.biomac.9b01204 · Cited by 51
Goodarzi, K., Shariatzadeh, F. J., Solouk, A., Akbari, S., & Mirzadeh, H. (2020) · European Polymer Journal · doi:10.1016/j.eurpolymj.2020.109992 · Cited by 52
Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress
Pedroza-González, S. C., Rodríguez-Salvador, M., Pérez-Benítez, B. E., Álvarez, M. M., & Santiago, G. T. (2021) · International Journal of Bioprinting · doi:10.18063/ijb.v7i2.337 · Cited by 51
Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering
Quint, J. P., Samandari, M., Abbasi, L., Lara, E. C. M., Rinoldi, C., Mostafavi, A., & Tamayol, A. (2021) · Nanoscale · doi:10.1039/d1nr06143g · Cited by 52
Self-citation3D bioprinted tumor model with extracellular matrix enhanced bioinks for nanoparticle evaluation
Chen, Y., Xu, L., Li, W., Chen, W., He, Q., Zhang, X., Tang, J., Wang, Y., Liu, B., & Liu, J. (2022) · Biofabrication · doi:10.1088/1758-5090/ac48e4 · Cited by 51
“The method of preparing the GelMA was based on the previous report [37].”
Development of gelatin methacrylate (GelMa) hydrogels for versatile intracavitary applications
Kulkarni, N. S., Chauhan, G., Goyal, M., Sarvepalli, S., & Gupta, V. (2022) · Biomaterials Science · doi:10.1039/d2bm00022a · Cited by 50
Bedell, M. L., Torres, A. L., Hogan, K. J., Wang, Z., Wang, B., Melchiorri, A. J., Grande-Allen, K. J., & Mikos, A. G. (2022) · Biofabrication · doi:10.1088/1758-5090/ac8768 · Cited by 53
“…anhydride, though adjusting the many different aspects of the synthesis procedure including molar excess of reactants, the use of acetic anhydride, and stirring speed may affect the final degree of modification and consequent functional properties of the polymer within a particular bioink [22–24].”
Microspheres in bone regeneration: Fabrication, properties and applications
Cai, Z., Jiang, H., Lin, T., Wang, C., Ma, J., Gao, R., Jiang, Y., & Zhou, X. (2022) · Materials Today Advances · doi:10.1016/j.mtadv.2022.100315 · Cited by 52
Advancements in drug-loaded hydrogel systems for bone defect repair
Shi, W., Jiang, Y., Wu, T., Zhang, Y., & Li, T. (2023) · Regenerative Therapy · doi:10.1016/j.reth.2023.12.010 · Cited by 48
“Considering the rich side chain groups of Gel, they can be modi fi ed by coupling with various substances to synthesize composite materials and subsequently overcome their shortcomings [12,13].”
BackgroundBiomaterials-Based Antioxidant Strategies for the Treatment of Oxidative Stress Diseases
Pérez-Araluce, M., Jüngst, T., Sanmartín, C., Prósper, F., Plano, D., & Mazo, M. M. (2024) · Biomimetics · doi:10.3390/biomimetics9010023 · Cited by 52
“Among them, natural polymers like collagen and gelatine (denatured/digested collagen) have been extensively used to produce diverse semi-synthetic materials [36], such as gelatine methacryloyl (GelMA) [37].”
BackgroundOral administration microrobots for drug delivery
Ren, A., Hu, J., Qin, C., Xia, N., Yu, M., Xu, X., Yang, H., Han, M. Y., Zhang, L., & Ma, L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.05.005 · Cited by 54
“Most applications of GelMA are GelMA-based hydrogels, which can be crosslinked by photoinitiated radical polymerization (i.e. under UV light exposure with the presence of a photo-initiator) [55, 58,59].”
BackgroundFrom Molecules to Machines: A Multiscale Roadmap to Intelligent, Multifunctional Soft Robotics
Li, Y., Schreiber, S., Yang, H., Liu, M., Little, J. M., Cao, W., Luo, Y., Bao, Y., Shih, C., Bai, H., et al. (2025) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00972 · Cited by 53
Engineering Microvascular Networks in LED Light-Cured Cell-Laden Hydrogels
Monteiro, N., He, W., França, C. M., Athirasala, A., & Bertassoni, L. E. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00502 · Cited by 49
Santiago, G. T.-D., Álvarez, M. M., Samandari, M., Prakash, G., Chandrabhatla, G., Rellstab-Sánchez, P. I., Byambaa, B., Abadi, P., Mandla, S., Avery, R., et al. (2018) · Materials Horizons · doi:10.1039/c8mh00344k · Cited by 37
Self-citationJaymand, M. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00802 · Cited by 50
Krishnamoorthy, S., Wadnap, S., Noorani, B., Xu, H., & Xu, C. (2020) · European Polymer Journal · doi:10.1016/j.eurpolymj.2020.109487 · Cited by 49
Parthiban, S. P., Athirasala, A., Tahayeri, A., Abdelmoniem, R., George, A., & Bertassoni, L. E. (2021) · Biofabrication · doi:10.1088/1758-5090/abb11f · Cited by 49
“We hypothesized that, similar to other popular photocross-linkable biomaterials, such as gelatin methacryloyl (GelMA) [19, 20] or methacrylated hyaluronic acid (MeHA) [21], photopolymerization of bone ECM proteins using these light sensitive moieties would enable formation of hydrogel scaffolds…”
Local delivery strategies to restore immune homeostasis in the context of inflammation
Bentley, E. R., & Little, S. R. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113971 · Cited by 48
Wu, H., Shang, Y., Sun, W., Ouyang, X., Zhou, W., Lu, J., Shuhui, Y., Wei, W., Yao, X., Wang, X., et al. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.03.038 · Cited by 49
Martyniak, K., Lokshina, A., Cruz, M. A., Karimzadeh, M., Kemp, R., & Kean, T. J. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.08.058 · Cited by 51
Pamplona, R., González‐Lana, S., Romero, P., Ochoa, I., Martín‐Rapún, R., & Sánchez‐Somolinos, C. (2023) · ACS Applied Polymer Materials · doi:10.1021/acsapm.2c01980 · Cited by 52
Gu, Y., Zhang, L., Du, X., Fan, Z., Wang, L., Sun, W., Cheng, Y., Zhu, Y., & Chen, C. (2018) · Journal of Biomaterials Applications · doi:10.1177/0885328218805864 · Cited by 53
Honarpardaz, A., Irani, S., Pezeshki‐Modaress, M., Zandi, M., & Sadeghi, A. (2018) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36501 · Cited by 49
Chen, M., Zhang, Y., Xie, Q., Zhang, W., Pan, X., Gu, P., Zhou, H., Gao, Y., Walther, A., & Fan, X. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00642 · Cited by 49
Ha, M., Athirasala, A., Tahayeri, A., Menezes, P. D. P., & Bertassoni, L. E. (2019) · Dental Materials · doi:10.1016/j.dental.2019.10.013 · Cited by 52
Review of Design Considerations for Brain-on-a-Chip Models
Cameron, T., Bennet, T. J., Rowe, E. M., Anwer, M., Wellington, C. L., & Cheung, K. C. (2021) · Micromachines · doi:10.3390/mi12040441 · Cited by 49
“However, gelatin’s need for chemical crosslinking can sometimes lead to local cytotoxicity, and its poor thermal stability at body temperature [253] led to the widespread use of methacrylamide-modified gelatin (GelMA) [254].”
Background“All‐in‐One” Gel System for Whole Procedure of Stem‐Cell Amplification and Tissue Engineering
He, Q., Liao, Y., Zhang, J., Yao, X., Zhou, W., Hong, Y., & Ouyang, H. (2020) · Small · doi:10.1002/smll.201906539 · Cited by 48
Choi, E., Kim, D., Kang, D., Yang, G. H., Jung, B., Yeo, M., Park, M.-J., An, S., Lee, K., Kim, J. S., et al. (2021) · Regenerative Biomaterials · doi:10.1093/rb/rbab001 · Cited by 49
“Gelatin is easily dissolved in water and acetic acid, and due to its excellent biocompatibility, it is widely used in the field of tissue engineering [18, 19].”
BackgroundHydrogel-Based Scaffolds in Oral Tissue Engineering
Ayala-Ham, A. D. R., López-Gutiérrez, J., Bermúdez, M., Aguilar‐Medina, M., Sarmiento‐Sánchez, J. I., López‐Camarillo, C., Sánchez‐Schmitz, G., & Ramos‐Payán, R. (2021) · Frontiers in Materials · doi:10.3389/fmats.2021.708945 · Cited by 48
“Nowadays, methacrylate gelatin HG (GelMA), is also used and polymerizes under ultraviolet light, in the presence of a photoinitiator, in a thermosetting cross-linked HG (Yue et al., 2015).”
BackgroundHuman Organs‐on‐Chips: A Review of the State‐of‐the‐Art, Current Prospects, and Future Challenges
Zarrintaj, P., Saeb, M. R., Stadler, F. J., Yazdi, M. K., Nasirinezhad, M., Mohebbi, S., Seidi, F., Saeb, M. R., & Mozafari, M. (2021) · Advanced Biology · doi:10.1002/adbi.202000526 · Cited by 49
Metadata
- Authors
- Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Annabi, Nasim
- Khademhosseini, Ali
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 73
- Pages
- 254-271
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC4610009
- OpenAlex
- W1120477740
- Semantic Scholar
- db0131b27b7eaf183c929a73ccd69d06de5cc188
- Cited by
- 3,146
- References
- 111
- Citations by year
- 2026: 433 · 2025: 464 · 2024: 394 · 2023: 430 · 2022: 347 · 2021: 314 · 2020: 243 · 2019: 213 · 2018: 137 · 2017: 106 · 2016: 60 · 2015: 3
- FWCI
- 7.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biomaterials (acceptedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Health and Medical Research Council
- National Science Foundation (EFRI-1240443)
- IMMODGEL (602694)
- National Institutes of Health (EB012597, AR057837, DE021468, HL099073, AI105024, AR063745)
- Tecnológico de Monterrey
- Consejo Nacional de Ciencia y Tecnología, México-CONACyT (262130)
- CONACyT-Sistema Nacional de Investigadores (26048)
- MIT International Science and Technology Initiatives (MISTI)
- Fundación México en Harvard
- CONACYT (234713)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- gelatin methacryloyl, GelMA hydrogel, hydrogel synthesis, photopolymerization, extracellular matrix mimics, cell adhesion, tissue engineering scaffolds, bone tissue engineering, cartilage tissue engineering, cardiac tissue engineering, vascular tissue engineering, bioprinting, microfabrication, hybrid hydrogels, carbon nanotubes, graphene oxide, drug delivery, gene delivery, biosensing
- MeSH terms
- Animals, Biocompatible Materials, Extracellular Matrix, Gelatin, Graphite, Humans, Methacrylates, Oxides, Polymers, Hydrogels, Amino Acid Motifs, Tissue Engineering, Nanotubes, Carbon, Microfluidics, Cell Proliferation, Mice, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
