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Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels
Open accessAbstract
Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.
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“To this end, considerable efforts were made in developing hydrogel-based inks or printing techniques to enhance 3D stability and shape fidelity, such as printing multimaterial inks with combined nozzle system24, 25, photo-crosslinkable hydrogels17, 26, 27, adding viscosifiers28–30, or printing in sacrificial supporting materials31–33.”
From Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles (2023) · cites this paper as [17]
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Cites
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · cited 10×
“Specifically, the RGD motifs do not contain groups that will react with MA, which ensures the retention of good cell adhesive properties of the GelMA [6, 19, 29].”
BackgroundMethodInfluentialSelf-citation3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
Kolesky, D. B., Truby, R. L., Gladman, A. S., Busbee, T. A., Homan, K. A., & Lewis, J. A. (2014) · Advanced Materials · doi:10.1002/adma.201305506 · Cited by 2,102 · cited 3×
“[64] Kolesky DB, Truby RL, Gladman AS, Busbee TA, H oman KA, Lewis JA.”
BackgroundMethodInfluentialStructural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000) · Biomacromolecules · doi:10.1021/bm990017d · Cited by 1,536 · cited 6×
“Co nveniently, introduction of methacryloyl substituent groups confers to gelatin the property of photocrosslinking with the assistance of a photoinitiator and exposure to light, due to the photopolymerization of the methacryloyl substituents [14].”
BackgroundInfluential25th Anniversary Article: Rational Design and Applications of Hydrogels in Regenerative Medicine
Annabi, N., Tamayol, A., Uquillas, J. A., Akbari, M., Bertassoni, L. E., Cha, C., Camci‐Unal, G., Dokmeci, M. R., Peppas, N. A., & Khademhosseini, A. (2014) · Advanced Materials · doi:10.1002/adma.201303233 · Cited by 1,409 · cited 4×
“Hydrogels provide a versatile platform to include desired combinations of properties for designed applications [1].”
BackgroundMethodInfluentialSelf-citationFairbanks, B. D., Schwartz, M. P., Bowman, C. N., & Anseth, K. S. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.08.055 · Cited by 1,206 · cited 5×
“[33] Fairbanks BD, Schwartz MP, Bowman CN, Anseth K S.”
BackgroundInfluentialBilliet, T., Gevaert, E., De Schryver, T., Cornelissen, M., & Dubruel, P. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.09.078 · Cited by 1,015 · cited 2×
“gelatin [14], or gelatin methacrylamide [15-18] in literature by different authors.”
BackgroundBiochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9)
den Steen, P. E. V., Dubois, B., Nelissen, I., Rudd, P., Dwek, R., & Opdenakker, G. (2002) · Critical reviews in biochemistry and molecular biology · doi:10.1080/10409230290771546 · Cited by 948 · cited 1×
“[20] Van den Steen PE, Dubois B, Nelissen I, Rudd P M, Dwek RA, Opdenakker G.”
BackgroundCarbon-Nanotube-Embedded Hydrogel Sheets for Engineering Cardiac Constructs and Bioactuators
Shin, S. R., Jung, S. M., Zalabany, M., Kim, K., Zorlutuna, P., Kim, S. B., Nikkhah, M., Khabiry, M., Azize, M., Kong, J., et al. (2013) · ACS Nano · doi:10.1021/nn305559j · Cited by 946 · cited 5×
“Hybrid hydrogels based on GelMA and other biopolymers The fabrication and use of GelMA hybrids with other biopolymers has been explored for diverse purposes, including enhancing mechanical pr operties [85, 86], tuning characteristics such as porosity, swelling ratio and degradability [86], or imparting a particular pr…”
BackgroundMethodInfluentialSelf-citationHydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs
Bertassoni, L. E., Cecconi, M., Manoharan, V., Nikkhah, M., Hjortnaes, J., Cristino, A. L., Barabaschi, G., Demarchi, D., Dokmeci, M. R., Yang, Y., et al. (2014) · Lab Chip · doi:10.1039/c4lc00030g · Cited by 944 · cited 5×
“The sacrificial agarose segments could be eas ily removed from the GelMA matrix by simply pulling of the agarose cords, resulting in a bioprinted GelMA scaffold with internal hollow “vessels” [63].”
BackgroundMethodInfluentialSelf-citationPhotocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering
Zhao, X., Lang, Q., Yildirimer, L., Lin, Z. Y., Cui, W., Annabi, N., Ng, K. W., Dokmeci, M. R., Ghaemmaghami, A. M., & Khademhosseini, A. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500005 · Cited by 839 · cited 2×
“[104] Zhao X, Lang Q, Yildirimer L, Lin ZY, Cui W, Annabi N, et al.”
BackgroundSelf-citation
Cited by
Sorted by citations
Items 901–1,000 of 1,004
Yang, Y., Du, T., Zhang, J., Kang, T., Luo, L., Tao, J., Gou, Z., Chen, S., Du, Y., He, J., et al. (2017) · Advanced Science · doi:10.1002/advs.201600491 · Cited by 43
Visible light-based stereolithography bioprinting of cell-adhesive gelatin hydrogels
Wang, Z., Tian, Z., Jin, X., Holzman, J. F., Ménard, F., & Kim, K. (2017) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc.2017.8037144 · Cited by 43
Das, D., Cho, H., Kim, N., Phạm, H. T. T., Kim, I. G., Chung, E., & Noh, I. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.12.020 · Cited by 43
Biomedical Applications of Nanoparticles
Bharathala, S., & Sharma, P. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-818823-1.00008-9 · Cited by 44
Sharifi, F., Irani, S., Azadegan, G., Pezeshki‐Modaress, M., Zandi, M., & Saeed, M. (2020) · Bioactive Carbohydrates and Dietary Fibre · doi:10.1016/j.bcdf.2020.100215 · Cited by 43
Heterogeneous Structural Color Microfibers for Cardiomyocytes Tug‐of‐War
Chen, Z., Yu, Y., Guo, J., Sun, L., & Zhao, Y. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202007527 · Cited by 43
Modeling the Mechanobiology of Cancer Cell Migration Using 3D Biomimetic Hydrogels
Morales, X., Cortés‐Domínguez, I., & Ortíz-de-Solórzano, C. (2021) · Gels · doi:10.3390/gels7010017 · Cited by 47
“atin hydrogels display high retaining affinity for soluble growth factors [46].”
BackgroundToward Designing of Anti-infective Hydrogels for Orthopedic Implants: From Lab to Clinic
Garg, D., Matai, I., & Sachdev, A. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01408 · Cited by 43
Biological signal integrated microfluidic hydrogel microspheres for promoting bone regeneration
Zhao, Z., Li, R., Ruan, H., Cai, Z., Zhuang, Y., Han, Z., Zhang, M., Cui, W., & Cai, M. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.135176 · Cited by 43
Rashid, T. U., Sharmeen, S., Biswas, S., Ahmed, T., Mallik, A. K., Shahruzzaman, M., Sakib, M. N., Haque, P., & Rahman, M. M. (2019) · Polymers and polymeric composites · doi:10.1007/978-3-319-77830-3_53 · Cited by 43
Engineering injectable, biocompatible, and highly elastic bioadhesive cryogels
Rana, D., Colombani, T., Saleh, B., Mohammed, H. S., Annabi, N., & Bencherif, S. A. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100572 · Cited by 42
“To prepare HA and gelatin amenable to polymerization, these biopolymers were methacrylated as previously described [9,43].”
Self-citation3D printed elastic hydrogel conduits with 7,8-dihydroxyflavone release for peripheral nerve repair
Wu, W., Dong, Y., Liu, H., Jiang, X., Yang, L., Luo, J. C., Hu, Y., & Gou, M. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100652 · Cited by 42
Kurian, A. G., Mandakhbayar, N., Singh, R. K., Lee, J., Jin, G., & Kim, H. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100664 · Cited by 42
Gan, S., Zheng, Z., Zhang, M., Long, L., Zhang, X., Tan, B., Zhu, Z., Liao, J., & Chen, W. (2023) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.3c02528 · Cited by 43
Amirian, J., Wychowaniec, J. K., Zendehdel, E. A., Sharma, G., Brangule, A., & Bandere, D. (2023) · Biomacromolecules · doi:10.1021/acs.biomac.3c00098 · Cited by 42
Messaoud, G. B., Aveic, S., Wachendörfer, M., Fischer, H., & Richtering, W. H. (2023) · Small · doi:10.1002/smll.202208089 · Cited by 46
Asl, S. K., Rahimzadegan, M., & Asl, A. K. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.129924 · Cited by 43
Zhao, F., Zhang, Z., & Guo, W. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1356580 · Cited by 42
Leonard, A. R., Cumming, M. H., Ali, M. A., & Cabral, J. D. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202405335 · Cited by 42
A novel personalized 3D injectable protein scaffold for regenerative medicine
Anitua, E., Pino, A., Troya, M., Jaén, P., & Orive, G. (2017) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-017-6012-6 · Cited by 43
Khanmohammadi, M., Nemati, S., Ai, J., & Khademi, F. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.109808 · Cited by 42
3D printable non-isocyanate polyurethanes with tunable material properties
Warner, J. J., Wang, P., Mellor, W. M., Hwang, H. H., Park, J. H., Pyo, S., & Chen, S. (2019) · Polymer Chemistry · doi:10.1039/c9py00999j · Cited by 42
Material-based therapy for bone nonunion
Leng, Y., Yang, F., Wang, Q., Li, Z., Yuan, B., Peng, C., Ren, G., Wang, Z., Cui, Y., Wang, Y., et al. (2019) · Materials & Design · doi:10.1016/j.matdes.2019.108161 · Cited by 42
Hepburn, M. S., Wijesinghe, P., Major, L. G., Li, J., Mowla, A., Astell, C., Park, H. W., Hwang, Y., Choi, Y. S., & Kennedy, B. F. (2019) · Biomedical Optics Express · doi:10.1364/boe.383419 · Cited by 42
Biological perspectives and current biofabrication strategies in osteochondral tissue engineering
Vyas, C., Mishbak, H., Cooper, G., Peach, C., Pereira, R., & Bártolo, P. (2020) · Biomanufacturing Reviews · doi:10.1007/s40898-020-00008-y · Cited by 36
Impact of cell density on the bioprinting of gelatin methacrylate (GelMA) bioinks
Jain, T., Baker, H. B., Gipsov, A., Fisher, J. P., Joy, A., Kaplan, D. S., & Isayeva, I. S. (2021) · Bioprinting · doi:10.1016/j.bprint.2021.e00131 · Cited by 44
A flexible microfluidic strategy to generate grooved microfibers for guiding cell alignment
Zhao, M., Liu, H., Zhang, X., Wang, H., Tao, T., & Qin, J. (2021) · Biomaterials Science · doi:10.1039/d1bm00549a · Cited by 42
Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling
Braccini, S., Tacchini, C., Chiellini, E., & Puppi, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23063265 · Cited by 45
“Gelatin can be chemically modified through direct reaction with methacrylic anhydride (MA) in phosphate buffer solution (PBS) at 50 ◦C to obtain gelatin–methacryloyl (GelMA), which can be photocrosslinked to achieve high stability in physiological conditions [248].”
BackgroundAdvances in scaffolds used for pulp–dentine complex tissue engineering: A narrative review
Noohi, P., Abdekhodaie, M. J., Nekoofar, M. H., Galler, K. M., & Dummer, P. M. H. (2022) · International Endodontic Journal · doi:10.1111/iej.13826 · Cited by 44
Preparation strategy of hydrogel microsphere and its application in skin repair
Chi, H., Qiu, Y., Ye, X., Shi, J., & Li, Z. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1239183 · Cited by 43
“The photomask method is a new light “drilling” technology that uses light as a processing means of soft materials (Yue et al., 2015).”
MethodApplication of photo-crosslinkable gelatin methacryloyl in wound healing
Zhang, J., Liu, C., Li, X., Liu, Z., & Zhang, Z. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1303709 · Cited by 41
A 3D-Printed Scaffold for Repairing Bone Defects
Dong, J., Ding, H., Wang, Q. L., & Wang, L. (2024) · Polymers · doi:10.3390/polym16050706 · Cited by 41
Shu, F., Huang, H., Xiao, S., Xia, Z., & Zheng, Y. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.04.019 · Cited by 41
Advances in printing biomaterials and living cells
Yue, Z., Liu, X., Coates, P. T., & Wallace, G. G. (2016) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000346 · Cited by 45
“Rapid photo-crosslinking with high cell viability has been demonstrated in both UV and visible light-mediated systems under optimal conditions [23,24].”
BackgroundGelatin based dynamic hydrogels via thiol–norbornene reactions
Perera, M. M., & Ayres, N. (2017) · Polymer Chemistry · doi:10.1039/c7py01630a · Cited by 41
Niosomes encapsulated in biohydrogels for tunable delivery of phytoalexin resveratrol
Machado, N. D., Fernández, M. A., Häring, M., Saldías, C., & Díaz, D. D. (2019) · RSC Advances · doi:10.1039/c8ra09655d · Cited by 41
Ferritin Nanocage Conjugated Hybrid Hydrogel for Tissue Engineering and Drug Delivery Applications
Samanipour, R., Wang, T., Werb, M., Hassannezhad, H., Rangel, J. M. L., Hoorfar, M., Hasan, A., Lee, C. K., & Shin, S. R. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b01482 · Cited by 41
Dual-enzymatically crosslinked hyaluronic acid hydrogel as a long-time 3D stem cell culture system
Gao, F., Li, J., Wang, L., Zhang, D., Zhang, J., Guan, F., & Yao, M. (2020) · Biomedical Materials · doi:10.1088/1748-605x/ab712e · Cited by 41
3D-bioprinted all-inclusive bioanalytical platforms for cell studies
Mazrouei, R., Velasco, V., & Esfandyarpour, R. (2020) · Scientific Reports · doi:10.1038/s41598-020-71452-6 · Cited by 42
Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering
Shi, Z., Xu, Y., Mulatibieke, R., Zhong, Q., Pan, X., Chen, Y., Lian, Q., Luo, X., Shi, Z., & Zhu, Q. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s270681 · Cited by 41
RetractedEngineered Microgels—Their Manufacturing and Biomedical Applications
Alzanbaki, H., Moretti, M., & Hauser, C. A. E. (2021) · Micromachines · doi:10.3390/mi12010045 · Cited by 41
“GelMA has been prepared under highly controlled and consistent production methods, which strongly reduced batch-to-batch variations [8].”
BackgroundInfluentialKim, W.-J., Lee, H., Lee, C. K., Kyung, J. W., An, S. B., Han, I., & Kim, G. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202105170 · Cited by 40
Ghazali, H. S., Askari, E., Seyfoori, A., & Naghib, S. M. (2023) · Scientific Reports · doi:10.1038/s41598-023-35865-3 · Cited by 40
Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking
Kim, M. H., Nguyen, H. D., Chang, C., & Lin, C. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00709 · Cited by 41
Kim, E. M., Lee, G. M., Lee, S., Kim, S., Lee, D., Yoon, D. S., Joo, J., Kong, H., Park, H. H., & Shin, H. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.11.145 · Cited by 40
Gelatin Methacryloyl Hydrogels for Musculoskeletal Tissue Regeneration
Kim, Y., Dawson, J. I., Oreffo, R. O. C., Tabata, Y., Kumar, D., Aparicio, C., & Mutreja, I. (2022) · Bioengineering · doi:10.3390/bioengineering9070332 · Cited by 43
“The chemical modification of the gelatin backbone with methacrylic anhydride only utilizes 5% of the amino acid residues per molar ratio [11].”
BackgroundHwangbo, H., Lee, H., Jin, E., Jo, Y., Son, J., Woo, H. M., Ryu, D., & Kim, G. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202209157 · Cited by 39
Zhu, Q., Zhou, X., Zhang, Y., Ye, D., Yu, K., Cao, W., Zhang, L., Zheng, H., Sun, Z., Guo, C., et al. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00346-1 · Cited by 40
Nanomaterials-combined methacrylated gelatin hydrogels (GelMA) for cardiac tissue constructs
Lisboa, E. S., Serafim, C., Mota, W. S., dos Santos, V. L. S., Júnior, R. L. C. D. A., Chaud, M. V., Cardoso, J. C., Jain, S. A., Severino, P. B., & Souto, E. B. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.11.056 · Cited by 42
Walejewska, E., Melchels, F. P. W., Paradiso, A., McCormack, A., Szlązak, K., Olszewska, A., Srebrzynski, M., & Święszkowski, W. (2023) · Biomacromolecules · doi:10.1021/acs.biomac.3c00909 · Cited by 41
Microenvironment‐Responsive Injectable Conductive Hydrogel for Spinal Cord Injury Repair
Liu, Q., Wang, W., Yang, H., Wang, Y., Shi, Y., Chen, Y., Luo, D., Guo, D., Lin, D., Yue, K., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202406376 · Cited by 40
Self-citationClerkin, S., Singh, K., Davis, J. L., Treacy, N. J., Krupa, I., Reynaud, E. G., Lees, R. M., Needham, S. R., MacWhite-Begg, D., Wychowaniec, J. K., et al. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123349 · Cited by 42
Khattak, S., Ullah, I., Yousaf, M. T., Ullah, S., Yousaf, H., Li, Y., Jin, H., Shen, J., & Xu, H. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.147270 · Cited by 43
Multi-length scale bioprinting towards simulating microenvironmental cues
Gill, E., Li, X., Birch, M., & Huang, Y. Y. S. (2018) · Bio-Design and Manufacturing · doi:10.1007/s42242-018-0014-1 · Cited by 38
3D bioprinting of hydrogel‐based biomimetic microenvironments
Luo, Y., Wei, X., & Huang, P. (2018) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34262 · Cited by 41
Application of electrospun nanofibers in bone, cartilage and osteochondral tissue engineering
Ding, H., Cheng, Y., Niu, X., & Hu, Y. (2020) · Journal of Biomaterials Science Polymer Edition · doi:10.1080/09205063.2020.1849922 · Cited by 40
“It contains a lot of amino, carboxyl and hydroxyl groups [49].”
BackgroundChen, Y., Chen, Y., Xiong, X., Cui, R., Zhang, G., Wang, C., Xiao, D., Qu, S., & Weng, J. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100261 · Cited by 41
Hybrid Hydrogels Based on Methacrylate-Functionalized Gelatin (GelMA) and Synthetic Polymers
Liang, J., Dijkstra, P. J., Poot, A. A., & Grijpma, D. W. (2022) · Biomedical Materials & Devices · doi:10.1007/s44174-022-00023-2 · Cited by 40
“Hydrogels prepared from unmodified gelatin often cannot meet the requirements of medical materials as their thermal stability, degradation characteristics, and mechanical properties are sub-optimal [18, 21].”
BackgroundInfluentialHiraki, H. L., Matera, D. L., Wang, W. Y., Prabhu, E. S., Zhang, Z., Midekssa, F. S., Argento, A. E., Buschhaus, J. M., Humphries, B. A., Luker, G. D., et al. (2021) · SSRN Electronic Journal · doi:10.1016/j.actbio.2022.09.043 · Cited by 40
Zidan, G., Greene, C. A., Etxabide, A., Rupenthal, I. D., & Seyfoddin, A. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.120452 · Cited by 39
Meng, Q., Wang, Y., Li, Y., & Shen, C. (2020) · Biotechnology and Bioengineering · doi:10.1002/bit.27589 · Cited by 39
“Moreover, the coating well supported the proliferation of HUVECs during 6 days of culture (Figure S3E), suggesting the good cytocompatibility of Gel‐MA that has been widely proved by the previous studies (Liu & Chan‐Park, 2009; Yue et al., 2015).”
ResultFree Radical Scavenging Composite Gelatin Methacryloyl Hydrogels for Cell Encapsulation
Lee, G. M., Kim, S., Kim, E. M., Kim, E., Lee, S., Lee, E.-J., Park, H. H., & Shin, H. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.06.043 · Cited by 39
Grieco, M., Ursini, O., Palamà, I. E., Gigli, G., Moroni, L., & Cortese, B. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100453 · Cited by 40
Agarwal, K., Srinivasan, V., Lather, V., Pandita, D., & Vasanthan, K. S. (2022) · Journal of materials research/Pratt's guide to venture capital sources · doi:10.1557/s43578-022-00524-2 · Cited by 39
“5 × 106 cells/mL for more than eight days [12].”
Background3D bioprinted liver tissue and disease models: Current advances and future perspectives
Sun, L., Wang, Y., Zhang, S., Yang, H., & Mao, Y. (2023) · Biomaterials Advances · doi:10.1016/j.bioadv.2023.213499 · Cited by 39
Zhu, A., Xu, Q., Huang, J., Li, Y., Zhang, F., Qin, S., Li, S., Wan, C., & Xie, H. (2023) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.3c06966 · Cited by 39
Zhu, Z., Ye, H., Zhang, K., He, G., Pan, Z., Xian, Y., Yang, Y. P., Zhang, C., & Wu, D. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.4c00105 · Cited by 39
3D‐Printed Dendritic Cell Vaccines for Post‐Surgery Cancer Immunotherapy
Xu, Y., Zhu, W., Wu, J., Liu, L., Yue, L., Zhang, X., Li, J., She, P., Yang, J., Sun, C., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202400507 · Cited by 39
Iqbal, M. Z., Riaz, M., Biedermann, T., & Klar, A. S. (2024) · Angiogenesis · doi:10.1007/s10456-024-09928-6 · Cited by 45
Biomaterial-assisted organoid technology for disease modeling and drug screening
Shao, Y., Wang, J., Jin, A., Jiang, S., Lei, L., & Liu, L. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101438 · Cited by 45
Synthesis and processing of hydrogels for medical applications
Redaelli, F., Sorbona, M., & Rossi, F. (2016) · Elsevier eBooks · doi:10.1016/b978-0-08-100262-9.00010-0 · Cited by 39
Fabrication of hydrogel scaffolds via photocrosslinking of methacrylated silk fibroin
Bessonov, I. V., Rochev, Y. A., Arkhipova, A. Y., Kopitsyna, M. N., Bagrov, D. V., Karpushkin, E. A., Bibikova, T. N., Moysenovich, A. M., Солдатенко, А. С., Nikishin, I. I., et al. (2019) · Biomedical Materials · doi:10.1088/1748-605x/ab04e0 · Cited by 39
Tsai, C.-C., Kuo, S.-H., Lu, T.-Y., Cheng, N., Shie, M., & Yu, J. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b01756 · Cited by 39
3D printing and bioprinting using multiphoton lithography
Zuev, D. M., Nguyen, A. K., Putlyaev, V. I., & Narayan, R. J. (2020) · Bioprinting · doi:10.1016/j.bprint.2020.e00090 · Cited by 41
Lim, W., Shin, S. Y., Cha, J. M., & Bae, H. (2021) · Polymers · doi:10.3390/polym13111773 · Cited by 40
Zhang, Y., Yin, P., Huang, J., Yang, L., Liu, Z., Fu, D., Hu, Z., Huang, W., & Miao, Y. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01671-8 · Cited by 39
“Degradation test The degradation test was performed according to a previously described protocol [70].”
MethodWei, L., Huang, J. S., Yan, Y., Cui, J., Zhao, Y., Bai, F., Liu, J., Wu, X., Zhang, X., & Du, M. (2022) · ACS Applied Polymer Materials · doi:10.1021/acsapm.2c00482 · Cited by 40
Bioprinting using PEGDMA-based hydrogel on DLP printer
Chang, S., Ching, T., & Hashimoto, M. (2022) · Materials Today Proceedings · doi:10.1016/j.matpr.2022.09.017 · Cited by 38
An Adhesive Bioink toward Biofabrication under Wet Conditions
Li, W., Wang, M., Wang, S., Wang, X., Avila, A., Kuang, X., Mu, X., Garciamendez‐Mijares, C. E., Jiang, Z., Manríquez, J., et al. (2023) · Small · doi:10.1002/smll.202205078 · Cited by 39
BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair
Zheng, K., Zheng, X., Yu, M., He, Y., & Wu, D. (2023) · Journal of Functional Biomaterials · doi:10.3390/jfb14010039 · Cited by 41
“GelMA was synthesized according to previous protocols [5,9].”
BackgroundMethodInfluentialRusu, A. G., Nita, L. E., Simionescu, N., Ghilan, A., Chiriac, A. P., & Mititelu-Tarțău, L. (2023) · Polymers · doi:10.3390/polym15030780 · Cited by 38
“Over the years, methacryloyl gelatin electrospun 3D fibrous scaffolds (GelMA) with elasticity and tunable degradation rate have been successfully designed as biomaterials for tissue engineering or drug delivery [8,9].”
BackgroundSztankovics, D., Moldvai, D., Petővári, G., Gelencsér, R., Krencz, I., Raffay, R. E., Dankó, T., & Sebestyén, A. R. (2023) · Pathology & Oncology Research · doi:10.3389/pore.2023.1610996 · Cited by 39
Sun, Y., Yang, L., Du, L., Zhou, Y., Xu, K., Chen, J., He, Y., Qu, Q., Miao, Y., Xing, M. M. Q., et al. (2023) · Journal of Advanced Research · doi:10.1016/j.jare.2023.02.014 · Cited by 39
“C via the direct reaction of gelatin with MA [78].”
BackgroundFan, Z., Wei, X., Chen, K., Wang, L., & Xu, M. (2023) · Micromachines · doi:10.3390/mi14040878 · Cited by 41
“On the contrary, the physical and biochemical characteristics of synthetic hydrogels largely depend on their composition, polymerization method, and cross-linking density, providing greater coordination and mechanical strength in the manufacturing process [34].”
BackgroundMa, X., Zhou, W., Zhang, R., Zhang, C., Yan, J., Feng, J. C., Rosenholm, J. M., Shi, T., Shen, X., & Zhang, H. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100663 · Cited by 38
A Multidrug Delivery Microrobot for the Synergistic Treatment of Cancer
Li, Y., Dong, D., Qu, Y., Li, J., Chen, S., Zhao, H. Q., Zhang, Q., Jiao, Y., Fan, L., & Sun, D. B. (2023) · Small · doi:10.1002/smll.202301889 · Cited by 38
Scalable Generation of Pre‐Vascularized and Functional Human Beige Adipose Organoids
Escudero, M., Vaysse, L., Eke, G., Peyrou, M., Villarroya, F., Bonnel, S., Jeanson, Y., Boyer, L., Christophe Vieu, A., Chaput, B., et al. (2023) · Advanced Science · doi:10.1002/advs.202301499 · Cited by 38
Natural Polymeric Hydrogels Encapsulating Small Molecules for Diabetic Wound Healing
Oprita, E. I., Iosăgeanu, A., & Crăciunescu, O. (2023) · Gels · doi:10.3390/gels9110867 · Cited by 38
“…biocompatibility, but also antibacterial and antioxidant properties and modulation of inflammation at the wound situs to a higher extent compared to traditional dressings, affording promising future applications in diabetic wound healing [17,72] ( promotes cell adhesion mediated by integrins [70].”
BackgroundNatural Polymer–Based Hydrogel Dressings for Wound Healing
Cai, C., Li, W., Zhang, X., Cheng, B., Chen, S., & Zhang, Y. (2024) · Advances in Wound Care · doi:10.1089/wound.2024.0024 · Cited by 39
“To this end, modi fi ed gelatins are typically obtained by reacting methacrylates, 20 polyepox-ides, 21 aldehydes, 22 and isocyanates 23 with the carboxylic acid and amine groups that are abundant in gelatin to build and modulate the network of the gelatin-based hydrogel.”
Biopolymer hydrogels and synergistic blends for tailored wound healing
Haririan, Y., & Asefnejad, A. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.135519 · Cited by 40
Huang, R., Sun, W., Li, W., Hu, R. H., Meng, R., Peng, Z., Yang, R., Huang, T., Du, J., Shang, L., et al. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.156869 · Cited by 38
Stoecker, L., Cedillo‐Servín, G., König, N. F., de Graaf, F. V., García‐Jiménez, M., Hofmann, S., Ito, K., Wentzel, A. S., & Castilho, M. D. (2025) · Advanced Materials · doi:10.1002/adma.202410292 · Cited by 42
3D bioprinted scaffolds for osteochondral regeneration: advancements and applications
Lu, J., Gao, Y. P., Cao, C., Wang, H., Ruan, Y., Qin, K., Liu, H., Wang, Y., Yang, P., Liu, Y., et al. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.101834 · Cited by 51
“Gelatin is a collagen hydrolysate that contains numerous RGD sequences for cell adhesion and MMP target sequences for cell remodeling [72].”
Microfibers as Physiologically Relevant Platforms for Creation of 3D Cell Cultures
McNamara, M. C., Sharifi, F. J., Wrede, A. H., Kimlinger, D. F., Thomas, D., Wiel, J. B. V., Chen, Y., Montazami, R., & Hashemi, N. N. (2017) · Macromolecular Bioscience · doi:10.1002/mabi.201700279 · Cited by 39
3D Printing Applied to Tissue Engineered Vascular Grafts
Wenger, R., & Giraud, M. (2018) · Applied Sciences · doi:10.3390/app8122631 · Cited by 38
“It then cross-links when exposed to light irradiation to form a hydrogel, with mechanical properties tunable through the ratio of the photo-cross-linker present in the pre-polymer solution [85].”
BackgroundPhysical Interactions Strengthen Chemical Gelatin Methacryloyl Gels
Rebers, L., Granse, T., Tovar, G. E. M., Southan, A., & Borchers, K. (2019) · Gels · doi:10.3390/gels5010004 · Cited by 38
“GM is a gelatin derivative, which is widely investigated in the field of regenerative medicine [7,8].”
Zargar, S. M., Mehdikhani, M., & Rafienia, M. (2019) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911519876080 · Cited by 38
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Xie, M., Yu, K., Sun, Y., Shao, L., Nie, J., Gao, Q., Qiu, J., Fu, J., Chen, Z., & He, Y. (2019) · Journal of Visualized Experiments · doi:10.3791/60545 · Cited by 38
Effects of nanocellulose on alginate/gelatin bio-inks for extrusion-based 3D printing
Han, C., Wang, X., Ni, Z., Ni, Y., Huan, W., Lv, Y. A., & Bai, S. (2020) · BioResources · doi:10.15376/biores.15.4.7357-7373 · Cited by 38
“…such as RGD peptides, which improve cell responses, such as cell adhesion, proliferation, migration, and differentiation (Liu and Chan-Park 2010; Yue et al. 2015; Xu et al. 2019), but it is difficult to directly print gelatin scaffolds with extrusion-based 3D printing due to its low viscosity.”
BackgroundEhlen, Q. T., Costello, J. P., Mirsky, N. A., Slavin, B. V., Parra, M., Ptashnik, A., Nayak, V. V., Coelho, P. G., & Witek, L. (2024) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c01279 · Cited by 29
Metadata
- Authors
- Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Annabi, Nasim
- Khademhosseini, Ali
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 73
- Pages
- 254-271
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC4610009
- OpenAlex
- W1120477740
- Semantic Scholar
- db0131b27b7eaf183c929a73ccd69d06de5cc188
- Cited by
- 3,146
- References
- 111
- Citations by year
- 2026: 433 · 2025: 464 · 2024: 394 · 2023: 430 · 2022: 347 · 2021: 314 · 2020: 243 · 2019: 213 · 2018: 137 · 2017: 106 · 2016: 60 · 2015: 3
- FWCI
- 7.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biomaterials (acceptedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Health and Medical Research Council
- National Science Foundation (EFRI-1240443)
- IMMODGEL (602694)
- National Institutes of Health (EB012597, AR057837, DE021468, HL099073, AI105024, AR063745)
- Tecnológico de Monterrey
- Consejo Nacional de Ciencia y Tecnología, México-CONACyT (262130)
- CONACyT-Sistema Nacional de Investigadores (26048)
- MIT International Science and Technology Initiatives (MISTI)
- Fundación México en Harvard
- CONACYT (234713)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- gelatin methacryloyl, GelMA hydrogel, hydrogel synthesis, photopolymerization, extracellular matrix mimics, cell adhesion, tissue engineering scaffolds, bone tissue engineering, cartilage tissue engineering, cardiac tissue engineering, vascular tissue engineering, bioprinting, microfabrication, hybrid hydrogels, carbon nanotubes, graphene oxide, drug delivery, gene delivery, biosensing
- MeSH terms
- Animals, Biocompatible Materials, Extracellular Matrix, Gelatin, Graphite, Humans, Methacrylates, Oxides, Polymers, Hydrogels, Amino Acid Motifs, Tissue Engineering, Nanotubes, Carbon, Microfluidics, Cell Proliferation, Mice, Tissue Scaffolds
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