Cyanobacterial biomass as carbohydrate and nutrient feedstock for bioethanol production by yeast fermentation
Open accessAbstract
BACKGROUND: Microbial bioconversion of photosynthetic biomass is a promising approach to the generation of biofuels and other bioproducts. However, rapid, high-yield, and simple processes are essential for successful applications. Here, biomass from the rapidly growing photosynthetic marine cyanobacterium Synechococcus sp. PCC 7002 was fermented using yeast into bioethanol. RESULTS: The cyanobacterium accumulated a total carbohydrate content of about 60% of cell dry weight when cultivated under nitrate limitation. The cyanobacterial cells were harvested by centrifugation and subjected to enzymatic hydrolysis using lysozyme and two alpha-glucanases. This enzymatic hydrolysate was fermented into ethanol by Saccharomyces cerevisiae without further treatment. All enzyme treatments and fermentations were carried out in the residual growth medium of the cyanobacteria with the only modification being that pH was adjusted to the optimal value. The highest ethanol yield and concentration obtained was 0.27 g ethanol per g cell dry weight and 30 g ethanol L(-1), respectively. About 90% of the glucose in the biomass was converted to ethanol. The cyanobacterial hydrolysate was rapidly fermented (up to 20 g ethanol L(-1) day(-1)) even in the absence of any other nutrient additions to the fermentation medium. CONCLUSIONS: Cyanobacterial biomass was hydrolyzed using a simple enzymatic treatment and fermented into ethanol more rapidly and to higher concentrations than previously reported for similar approaches using cyanobacteria or microalgae. Importantly, as well as fermentable carbohydrates, the cyanobacterial hydrolysate contained additional nutrients that promoted fermentation. This hydrolysate is therefore a promising substitute for the relatively expensive nutrient additives (such as yeast extract) commonly used for Saccharomyces fermentations.
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Glycogen is similar in structure to amylopectin, and therefore hydrolysis of glycogen requires the same amylolytic enzymes used to break down starch.
“Glycogen is similar in structure to amylopectin, and therefore the hydrolysis of glycogen requires the same amylolytic enzymes, which are used to breakdown starch.”
From The Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol (2016) · cites this paper as Möllers et al., 2014
Two reports found that only two enzymes, α-amylase and glucoamylase, are sufficient to partially hydrolyze glycogen and allow subsequent fermentation to ethanol.
“In fact, two reports found that only two enzymes, i.e., α-amylase and glucoamylase, are sufficient to partially hydrolyze glycogen and allow subsequent fermentation to ethanol.”
From The Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol (2016) · cites this paper as Möllers et al., 2014
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- Authors
- Möllers, K. B., Cannella, D., Jørgensen, H., & Frigaard, N.-U.
- Affiliations
- University of Copenhagen (DK)
- Corresponding author
- Frigaard, Niels-Ulrik
- Venue
- Biotechnology for Biofuels
- Published
- 2014-04-17
- Volume
- 7
- Issue
- 1
- Pages
- 64-64
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC4022056
- OpenAlex
- W2151060130
- Cited by
- 215
- References
- 49
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 17 · 2023: 19 · 2022: 22 · 2021: 17 · 2020: 25 · 2019: 24 · 2018: 16 · 2017: 16 · 2016: 19 · 2015: 14 · 2014: 2
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, other-oa)
- Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion, other-oa)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, other-oa)
- Funders
- Nordic Energy Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- cyanobacterial biomass, Synechococcus sp. PCC 7002, bioethanol production, Saccharomyces cerevisiae, enzymatic hydrolysis, lysozyme, nitrate limitation, ethanol yield, yeast extract replacement
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