A biorefinery from Nannochloropsis sp. microalga – Extraction of oils and pigments. Production of biohydrogen from the leftover biomass
Open accessAbstract
The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33 g lipids/100 g dry biomass were found to be at 40 °C, 300 bar and a CO2 flow-rate of 0.62 g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45 g lipids/100 g dry biomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6 mL/g dry biomass.
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Stress conditions are unfavorable environmental factors, such as strong light, high salinity, high temperature, deprivation of nitrogen or other nutrients, short-term exposure to UV radiation, or a combination of these factors.
“Stress conditions are unfavorable environmental factors, such as strong light, high salinity, high temperature, deprivation of nitrogen or other nutrients, short-term exposure to UV radiation, or a combination of these factors [152].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [152]
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- Authors
- Nobre, B. P., Villalobos, F., Barragán, B. E., Oliveira, A. C., Batista, A. P., Marques, P. A. S. S., Mendes, R. L., Sovová, H., Palavra, A. F., & Gouveia, L.
- Affiliations
- National Laboratory of Energy and Geology (PT)
- Czech Academy of Sciences, Institute of Chemical Process Fundamentals (CZ)
- Corresponding author
- Gouveia, L.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 135
- Pages
- 128-136
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2053806498
- Cited by
- 333
- References
- 35
- Citations by year
- 2026: 17 · 2025: 20 · 2024: 23 · 2023: 26 · 2022: 31 · 2021: 33 · 2020: 35 · 2019: 22 · 2018: 30 · 2017: 29 · 2016: 24 · 2015: 19 · 2014: 13 · 2013: 11
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- ASEP (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Nannochloropsis, microalgae, biorefinery, supercritical CO2 extraction, lipid extraction, carotenoid extraction, ethanol co-solvent, biodiesel feedstock, biohydrogen, dark fermentation, Enterobacter aerogenes, microalgal biomass valorization
- MeSH terms
- Biotechnology, Chromatography, High Pressure Liquid, Fermentation, Hydrogen, Lipids, Oils, Pigments, Biological, Solvents, Biomass, Chromatography, Supercritical Fluid, Biofuels, Microalgae
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