Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta
Abstract
Laminaran and three fractions of fucoidan were isolated from brown alga Alaria angusta. The laminaran AaL was characterized as a typical 1,3;1,6-β-D-glucan (ratio of bonds 1,3:1,6 = 10:1). Fucoidans AaF1 and AaF2 are sulfated heteropolysaccharides, containing fucose, galactose, mannose and xylose. The fraction AaF3 is sulfated and acetylated galactofucan with the main chain represented by a repeating unit → 3)-α-L-Fucp-(2,4-SO3(-))-(1 →. According the data of methylation analysis, AaF3 contains mainly 1,3-linked fucose, less 1,4-linked and 1,4,6-linked galactose residues. The autohydrolysis (37 °C) of fucoidan AaF3 allowed to obtain selectively 2-desulfaled polysaccharide fraction, built up of fucose only, and low molecular weight (LMW) fraction. The negative-ion tandem mass spectrometry of LMW fraction, further hydrolyzed by acid hydrolysis identified the following fragments: Gal-2-SO3(-)-(1 → 4)-Gal, Gal-4-SO3(-)-(1 → 4)-Gal, Gal-(1 → 2)-Gal-4-SO3(-), Fuc-2-SO3(-)-(1 → 4)-Gal, Gal-2-SO3(-)-(1 → 3)-Fuc-(1 → 3)-Fuc, Fuc-2-SO3(-)-(1 → 3)-Fuc-(1 → 4)-Gal. The laminaran AaL and the fucoidan AaF3 exhibited no cytotoxicity in vitro for HT 29, T-47D, and SK-MEL-28 cell lines. The AaF3 fraction suppressed colony formation of HT 29 and T-47D cells, AaL-only HT 29 cells.
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Low molecular weight fucoidan from Alaria angusta has antiproliferative effects against HT 29 and T-47D cell lines, and a laminarin fraction has antiproliferative effects against HT 29 cell lines.
“Low molecular weight fucoidan fraction from Alaria angusta showed antiproliferative effects against HT 29 and T-47D cell lines, while laminarin fraction showed antiproliferative effects against HT 29 cell lines”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Menshova et al., 2015
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- Authors
- Menshova, R. V., Anastyuk, S. D., Ermakova, S. P., Shevchenko, N. M., Isakov, V. I., & Zvyagintseva, T. N.
- Affiliations
- Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences (RU)
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2015-11
- Volume
- 132
- Pages
- 118-125
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W564719654
- Cited by
- 78
- References
- 37
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 10 · 2023: 5 · 2022: 7 · 2021: 10 · 2020: 16 · 2019: 5 · 2018: 4 · 2017: 7 · 2016: 6
- FWCI
- 8.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Foundation for Basic Research (14-04-93003)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- laminarin, fucoidan, brown algae, polysaccharide structure, fucose, acetylated polysaccharides, methylation analysis, tandem mass spectrometry, anticancer activity, cytotoxicity, HT-29 cells, T47D cells, SK-MEL-28 cells, colony formation inhibition, autohydrolysis
- MeSH terms
- Phaeophyceae, Antineoplastic Agents, Glucans, Humans, Neoplasms, Polysaccharides, Cell Line, Tumor
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