Cited by 78Seaweed-derived Bioactive CompoundsPolysaccharides Composition and ApplicationsPolysaccharides and Plant Cell Walls

Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta

Menshova, Roza V., Anastyuk, Stanislav D., Ermakova, Svetlana P., Shevchenko, Natalia M., Isakov, Vladimir I., Zvyagintseva, Tatiana N.·2015·Carbohydrate Polymers

Abstract

Laminaran and three fractions of fucoidan were isolated from brown alga Alaria angusta. The laminaran AaL was characterized as a typical 1,3;1,6-β-D-glucan (ratio of bonds 1,3:1,6 = 10:1). Fucoidans AaF1 and AaF2 are sulfated heteropolysaccharides, containing fucose, galactose, mannose and xylose. The fraction AaF3 is sulfated and acetylated galactofucan with the main chain represented by a repeating unit → 3)-α-L-Fucp-(2,4-SO3(-))-(1 →. According the data of methylation analysis, AaF3 contains mainly 1,3-linked fucose, less 1,4-linked and 1,4,6-linked galactose residues. The autohydrolysis (37 °C) of fucoidan AaF3 allowed to obtain selectively 2-desulfaled polysaccharide fraction, built up of fucose only, and low molecular weight (LMW) fraction. The negative-ion tandem mass spectrometry of LMW fraction, further hydrolyzed by acid hydrolysis identified the following fragments: Gal-2-SO3(-)-(1 → 4)-Gal, Gal-4-SO3(-)-(1 → 4)-Gal, Gal-(1 → 2)-Gal-4-SO3(-), Fuc-2-SO3(-)-(1 → 4)-Gal, Gal-2-SO3(-)-(1 → 3)-Fuc-(1 → 3)-Fuc, Fuc-2-SO3(-)-(1 → 3)-Fuc-(1 → 4)-Gal. The laminaran AaL and the fucoidan AaF3 exhibited no cytotoxicity in vitro for HT 29, T-47D, and SK-MEL-28 cell lines. The AaF3 fraction suppressed colony formation of HT 29 and T-47D cells, AaL-only HT 29 cells.

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Crossref · 10.1016/j.carbpol.2015.06.020

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  • Low molecular weight fucoidan from Alaria angusta has antiproliferative effects against HT 29 and T-47D cell lines, and a laminarin fraction has antiproliferative effects against HT 29 cell lines.

    “Low molecular weight fucoidan fraction from Alaria angusta showed antiproliferative effects against HT 29 and T-47D cell lines, while laminarin fraction showed antiproliferative effects against HT 29 cell lines”

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Authors
Menshova, R. V., Anastyuk, S. D., Ermakova, S. P., Shevchenko, N. M., Isakov, V. I., & Zvyagintseva, T. N.
Affiliations
Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences (RU)
Venue
Carbohydrate Polymers
Publisher
Elsevier BV
Published
2015-11
Volume
132
Pages
118-125
ISSN
0144-8617 (ISSN-L)
OpenAlex
W564719654
Cited by
78
References
37
Citations by year
2026: 4 · 2025: 4 · 2024: 10 · 2023: 5 · 2022: 7 · 2021: 10 · 2020: 16 · 2019: 5 · 2018: 4 · 2017: 7 · 2016: 6
FWCI
8.81
Type
journal article
Language
en
Open access
closed
Locations
  • Carbohydrate Polymers (publishedVersion)
  • PubMed (publishedVersion)
Funders
Russian Foundation for Basic Research (14-04-93003)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
laminarin, fucoidan, brown algae, polysaccharide structure, fucose, acetylated polysaccharides, methylation analysis, tandem mass spectrometry, anticancer activity, cytotoxicity, HT-29 cells, T47D cells, SK-MEL-28 cells, colony formation inhibition, autohydrolysis
MeSH terms
Phaeophyceae, Antineoplastic Agents, Glucans, Humans, Neoplasms, Polysaccharides, Cell Line, Tumor

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