Exopolysaccharides of Lactobacillus reuteri: Their Influence on Adherence of E. Coli to Epithelial Cells and Inflammatory Response
Abstract
The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
High-performance anion-exchange chromatography coupled with pulsed amperometric detection or gas chromatography are commonly utilized to analyze the monosaccharide composition of EPS.
“High-performance, anion-exchange chromatography coupled with pulsed amperometric detection [8,27,35,53] or gas chromatography (GC) [32,43] are commonly utilized to analyze the monosaccharide composition of EPS.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [32]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269 · see citing works
A simple and rapid method for the permethylation of carbohydrates
Ciucanu, I., & Kerek, F. (1984) · Carbohydrate Research · doi:10.1016/0008-6215(84)85242-8 · Cited by 3,581 · see citing works
Englyst, H. N., & Cummings, J. H. (1984) · The Analyst · doi:10.1039/an9840900937 · Cited by 1,096 · see citing works
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells
Duhen, T., Geiger, R., Jarrossay, D., Lanzavecchia, A., & Sallusto, F. (2009) · Nature Immunology · doi:10.1038/ni.1767 · Cited by 1,079 · see citing works
Beg, A. A., Finco, T. S., Nantermet, P. V., & Baldwin, A. S. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.6.3301 · Cited by 1,027 · see citing works
PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples
Wang, R. F., Cao, W. W., & Cerniglia, C. E. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.4.1242-1247.1996 · Cited by 502 · see citing works
New Perspectives for Lactobacilli Exopolysaccharides
Badel, S., Bernardi, T., & Michaud, P. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.011 · Cited by 497 · see citing works
Detection of Mycoplasma in cell cultures
Young, L., Sung, J., Stacey, G., & Masters, J. R. (2010) · Nature Protocols · doi:10.1038/nprot.2010.43 · Cited by 437 · see citing works
Impaired expression of peroxisome proliferator-activated receptor γ in ulcerative colitis
Dubuquoy, L., Jansson, E. Å., Deeb, S. S., Rakotobé, S., Karoui, M., Colombel, J., Auwerx, J. H., Pettersson, S., & Desreumaux, P. (2003) · Gastroenterology · doi:10.1016/s0016-5085(03)00271-3 · Cited by 425 · see citing works
Cell Wall Structure and Function in Lactic Acid Bacteria
Chapot-Chartier, M.-P., & Kulakauskas, S. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-s1-s9 · Cited by 410 · see citing works
A PRACTICAL GUIDE TO THE METHYLATION ANALYSIS OF CARBOHYDRATES.
Jansson, P., Kenne, L., Liedgren, H., Lindberg, B., & Lōnngren, J. (1976) · Cited by 295 · see citing works
Ruas‐Madiedo, P., Gueimonde, M., Margollés, A., Reyes‐Gavilán, C. G. D. L., & Salminen, S. (2006) · Journal of Food Protection · doi:10.4315/0362-028x-69.8.2011 · Cited by 241 · see citing works
Immunomodulatory and antioxidant potential of <i>Lactobacillus</i> exopolysaccharides
Liu, C., Tseng, K., Chiang, S., Lee, B., Hsu, W., & Pan, T. (2011) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4456 · Cited by 230 · see citing works
Capsule Shields the Function of Short Bacterial Adhesins
Schembri, M. A., Dalsgaard, D., & Klemm, P. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.5.1249-1257.2004 · Cited by 217 · see citing works
Pham, P. L., Dupont, I., Roy, D., Lapointe, G., & Cerning, J. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2302-2310.2000 · Cited by 212 · see citing works
Antioxidant Activity of an Exopolysaccharide Purified from Lactococcus lactis subsp. lactis 12
Pan, D., & Mei, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.01.005 · Cited by 211 · see citing works
Arena, A., Maugeri, T. L., Pavone, B., Iannello, D., Gugliandolo, C., & Bisignano, G. (2005) · International Immunopharmacology · doi:10.1016/j.intimp.2005.07.004 · Cited by 207 · see citing works
Biosorption of heavy metals by the exopolysaccharide produced by Paenibacillus jamilae
Morillo, J. A., Ribera, R. G., Quesada, T., Aguilera, J., Alberto Ramos-cormenzana, A., & Monteoliva‐Sánchez, M. (2008) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-008-9800-9 · Cited by 200 · see citing works
van den Berg, D. J. C., Robijn, G. W., Janssen, A., Giuseppin, M. L. F., Vreeker, R., Kamerling, J. P., Vliegenthart, J. F. G., Ledeboer, A. M., & Verrips, C. T. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.8.2840-2844.1995 · Cited by 173 · see citing works
Cholesterol Lowering Activity of Ropy Fermented Milk
NAKAJIMA, H., SUZUKI, Y., & HIROTA, T. (1992) · Journal of Food Science · doi:10.1111/j.1365-2621.1992.tb06848.x · Cited by 170 · see citing works
Roselli, M., Finamore, A., Britti, M. S., Konstantinov, S. R., Smidt, H., de Vos, W. M., & Mengheri, E. (2007) · Journal of Nutrition · doi:10.1093/jn/137.12.2709 · Cited by 169 · see citing works
Hsu, H., Hua, K., Lin, C., Lin, C., Hsu, J., & Wong, C. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.10.5989 · Cited by 161 · see citing works
Microbial Exopolysaccharides - their Role in Microbial Adhesion in Aqueous Systems
Sutherland, I. W. (1982) · CRC Critical Reviews in Microbiology · doi:10.3109/10408418209113562 · Cited by 157 · see citing works
Surayot, U., Wang, J., Seesuriyachan, P., Kuntiya, A., Tabarsa, M., Lee, Y., Kim, J., Park, W., & You, S. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.05.005 · Cited by 141 · see citing works
Moue, M., Tohno, M., Shimazu, T., Kido, T., Aso, H., Saito, T., & Kitazawa, H. (2007) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2007.11.006 · Cited by 134 · see citing works
Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri 100-23
Sims, I. M., Frese, S. A., Walter, J., Loach, D. M., Wilson, M., Appleyard, K., Eason, J. R., Livingston, M. K., Baird, M. A., Cook, G. M., et al. (2011) · The ISME Journal · doi:10.1038/ismej.2010.201 · Cited by 123 · see citing works
van Leeuwen, S. S., Kralj, S., van Geel-Schutten, I. H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.01.042 · Cited by 121 · see citing works
Moineau, S., Borkaev, M., Holler, B. J., Walker, S. A., Kondo, J. K., Vedamuthu, E. R., & Vandenbergh, P. A. (1996) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(96)76584-0 · Cited by 118 · see citing works
López, P., Monteserín, D. C., Gueimonde, M., Reyes‐Gavilán, C. G. D. L., Margollés, A., Suárez, A., & Ruas‐Madiedo, P. (2011) · Food Research International · doi:10.1016/j.foodres.2011.11.020 · Cited by 113 · see citing works
Bleau, C., Monges, A., Rashidan, K. K., Laverdure, J., Lacroix, M., Calsteren, M. V., Millette, M., Savard, R., & Lamontagne, L. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04450.x · Cited by 108 · see citing works
Candela, M., Seibold, G. M., Vitali, B., Lachenmaier, S., Eikmanns, B. J., & Brigidi, P. (2005) · Research in Microbiology · doi:10.1016/j.resmic.2005.04.006 · Cited by 84 · see citing works
Guzmán-Murillo, M. A., & Ascencio, F. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2000.00751.x · Cited by 75 · see citing works
van Leeuwen, S. S., Kralj, S., van Geel-Schutten, I. H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.01.044 · Cited by 69 · see citing works
New method for exopolysaccharide determination in culture broth using stirred ultrafiltration cells
Bergmaier, D., Lacroix, C., Macedo, M. G., & Champagne, C. P. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100764 · Cited by 53 · see citing works
Functional Alteration of Macrophages by a Slime-Forming Lactococcus lactis ssp. cremoris
Kitazawa, H., Nomura, M., Itoh, T., & Yamaguchi, T. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78380-x · Cited by 27 · see citing works
Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli
Pavlová, B., Volf, J., Alexa, P., Rychlı́k, I., Matiašovic, J., & Faldyna, M. (2008) · Veterinary Microbiology · doi:10.1016/j.vetmic.2008.04.024 · Cited by 25 · see citing works
Chytilová, M., Nemcová, R., Gancarčíková, S., Mudroňová, D., & Tkáčiková, Ľ. (2014) · Acta Veterinaria Hungarica · doi:10.1556/avet.2014.024 · Cited by 12 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Role of Lactobacillus reuteri in Human Health and Diseases
Mu, Q., Tavella, V. J., & Luo, X. M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00757 · Cited by 744 · see citing works
“In addition, EPS synthesized by L. reuteri is able to inhibit E. coli adhesion to porcine epithelial cells in vitro (Ksonzekova et al., 2016).”
BackgroundExopolysaccharides of lactic acid bacteria: Structure, bioactivity and associations: A review
Zhou, Y., Cui, Y., & Qu, X. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.11.093 · Cited by 414 · see citing works
Jurášková, D., Ribeiro, S. C., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11020156 · Cited by 342 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
“In certain cases, heat treatment is used to improve the EPS recovery from the culture medium as the first step in EPS isolation [27,32,35,58,97,98].”
BackgroundMethodInfluentialAmiri, S., Mokarram, R. R., Khiabani, M. S., Bari, M. R., & Alizadeh, M. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.11.084 · Cited by 160 · see citing works
The Beneficial Role of Probiotic Lactobacillus in Respiratory Diseases
Du, T., Lei, A., Zhang, N., & Zhu, C. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.908010 · Cited by 147 · see citing works
Zhang, Z., Liu, Z., Tao, X., & Wei, H. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.07.084 · Cited by 142 · see citing works
Averina, O., Poluektova, E., Marsova, M., & Danilenko, V. (2021) · Biomedicines · doi:10.3390/biomedicines9101340 · Cited by 126 · see citing works
Zhang, J., Xiao, Y., Wang, H., Zhang, H., Chen, W., & Lu, W. (2023) · Microbiological Research · doi:10.1016/j.micres.2023.127432 · Cited by 140 · see citing works
Han, R., Wang, L., Zhao, Z., You, L., Pedišić, S., Kulikouskaya, V. I., & Lin, Z. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.106048 · Cited by 112 · see citing works
Pyclik, M. J., Šrůtková, D., Schwarzer, M., & Górska, S. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.12.227 · Cited by 92 · see citing works
Exopolysaccharides produced byLactobacillussp.: Biosynthesis and applications
Oleksy, M., & Klewicka, E. (2016) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2016.1187112 · Cited by 93 · see citing works
Probiotic Species in the Management of Periodontal Diseases: An Overview
Zhang, Y., Ding, Y., & Guo, Q. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.806463 · Cited by 80 · see citing works
Lactobacillus reuteri in digestive system diseases: focus on clinical trials and mechanisms
Peng, Y., Ma, Y., Luo, Z., Jiang, Y.-F., Xu, Z., & Yu, R. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2023.1254198 · Cited by 77 · see citing works
Hughes, K. R., Harnisch, L. C., Alcon-Giner, C., Mitra, S., Wright, C. J., Ketskemety, J., van Sinderen, D., Watson, A. J. M., & Hall, L. J. (2017) · Open Biology · doi:10.1098/rsob.160155 · Cited by 72 · see citing works
Disease managing capacities and mechanisms of host effects of lactic acid bacteria
Ren, C., Faas, M. M., & de Vos, P. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1758625 · Cited by 71 · see citing works
Yang, S., Xu, X., Peng, Q., Ma, L., Qiao, Y., & Shi, B. (2023) · Animal nutrition · doi:10.1016/j.aninu.2023.02.004 · Cited by 65 · see citing works
Chen, Y., Huang, X., Liu, A., Fan, S., Liu, S., Li, Z., Yang, X., Guo, H., Wu, M., Liu, M., et al. (2024) · Advanced Science · doi:10.1002/advs.202309725 · Cited by 63 · see citing works
Tarannum, N., Hossain, T. J., Ali, F., Das, T., Dhar, K., & Nafiz, I. H. (2023) · LWT · doi:10.1016/j.lwt.2023.115263 · Cited by 69 · see citing works
The multiple benefits of bioactive polysaccharides: From the gut to overall health
Huang, R., Zhang, J., Xu, X., Sun, M., Xu, L., Kuang, H., Xu, C., & Guo, L. (2024) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2024.104677 · Cited by 58 · see citing works
Pang, Y., Lundberg, L. E., Forsberg, M. M., Ahl, D., Bysell, H., Pallin, A., Sverremark‐Ekström, E., Karlsson, R., Jonsson, H., & Roos, S. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1032202 · Cited by 56 · see citing works
Jia, K., Tao, X., Liu, Z., Zhan, H., He, W., Zhang, Z., Zeng, Z., & Wei, H. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.12.245 · Cited by 56 · see citing works
Bene, K., Kavanaugh, D. W., Leclaire, C., Gunning, A. P., MacKenzie, D. A., Wittmann, A., Young, I. D., Kawasaki, N., Rajnavölgyi, É., & Juge, N. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00321 · Cited by 55 · see citing works
“reuteri strain DSM 17938 and L26 BiocenolTM was recently shown to exert up-regulation of the mRNA level of IL-1β, NF-κB, TNF-α, and IL-6 (Kšonžeková et al., 2016).”
Background’Ropy’ Phenotype, Exopolysaccharides and Metabolism: Study on Food Isolated Potential Probiotics LAB
Cirrincione, S., Breuer, Y., Mangiapane, E., Mazzoli, R., & Pessione, E. (2018) · Microbiological Research · doi:10.1016/j.micres.2018.07.004 · Cited by 53 · see citing works
“Ad-ditionally, it was shown that puri fi ed EPS from L. rhamnosus decreased pro-in fl ammatory cytokines (TNF, IL-6, IL-12) (K š on ž eková et al., 2016), whereas EPS from other LAB species exhibited immune-stimu-lation properties such as macrophage activation (Surayot et al., 2014).”
Liao, N., Pang, B., Han, J., Xu, X., Yan, L., Li, H., Shao, D., & Shi, J. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.04.022 · Cited by 48 · see citing works
Nemati, V., & Mozafarpour, R. (2024) · LWT · doi:10.1016/j.lwt.2024.116116 · Cited by 45 · see citing works
Improvement of inflammatory bowel disease by lactic acid bacteria-derived metabolites: a review
Guo, W.-L., Mao, B.-Y., Tang, X., Zhang, Q.-X., Zhao, J.-X., Zhang, H., Chen, W., & Cui, S.-M. (2023) · Critical reviews in food science and nutrition · doi:10.1080/10408398.2023.2291188 · Cited by 37 · see citing works
Cai, G., Liu, Y., Li, X., & Lu, J. Z. (2019) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.9b03234 · Cited by 42 · see citing works
Kristjansdottir, T., Bosma, E. F., dos Santos, F. B., Özdemir, E., Herrgård, M. J., França, L., Ferreira, B. S., Nielsen, A. T., & Guðmundsson, S. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1229-3 · Cited by 40 · see citing works
“The composition of lipoteichoic acid (Walter et al., 2007) and exopolysaccharides (Ksonzeková et al., 2016) in L. reuteri were obtained from literature.”
MethodZhang, J., Chen, X., Zhan, Q., Wang, Y., Meng, K., Hu, Q., & Zhao, L. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.140480 · Cited by 39 · see citing works
Liu, Y., Zheng, S., Cui, J., Guo, T., Zhang, J., & Li, B. (2021) · Microorganisms · doi:10.3390/microorganisms9102086 · Cited by 38 · see citing works
“Likewise, the anti-inflammatory potential of EPS has been widely studied in recent years [13].”
BackgroundHealth promoting benefits of postbiotics produced by lactic acid bacteria: Exopolysaccharide
Gezginç, Y., Erdem, T. K., Tatar, H. D., Ayman, S., Ganiyusufoğlu, E., & Dayısoylu, K. S. (2022) · Biotech Studies · doi:10.38042/biotechstudies.1159166 · Cited by 38 · see citing works
“They subsequently decreased ETEC-induced gene expression for the proinflammatory cytokines (IL-1, IL-6), which suggesting to role as a prophylactic effect to gastrointestinal infections (Kšonžeková et al., 2016).”
BackgroundButorac, K., Novak, J., Bellich, B., Terán, L. C., Banić, M., Pavunc, A. L., Zjalić, S., Cescutti, P., Šušković, J., & Kos, B. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01575-6 · Cited by 37 · see citing works
“EPS production affects the adhesion ability of Lactobacillus johnsonii, Lactobacillus rhamnosus GG and Lactobacillus reuteri studied in different in vitro models [2, 38, 39].”
BackgroundLimosilactobacillus reuteri Regulating Intestinal Function: A Review
Jiang, J., Li, K., Xiao, Y., Zhong, A., Tang, J., Duan, Y., & Li, Z. (2022) · Fermentation · doi:10.3390/fermentation9010019 · Cited by 36 · see citing works
“L. reuteri L26 can secrete an EPS (MW = 8.2 × 10 5 Da) composed of α -D-glucose (1 → 3) and (1 → 6) glycosidic bonds [67], which can interfere with the overexpression of calmodulin-dependent protein kinase II and the cAMP-dependent kinase protein-related gene PRKAR1A, which is induced by…”
BackgroundInfluentialCastro-Bravo, N., Hidalgo-Cantabrana, C., Rodríguez-Carvajal, M. Á., Ruas‐Madiedo, P., & Margollés, A. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01405 · Cited by 35 · see citing works
“This has been proved with in vitro (Ksonzeková et al., 2016; Zivkovic et al., 2016) as well as in vivo studies (Fanning et al.”
BackgroundYILDIRAN, H., Kılıç, G. B., & Karahan, A. G. (2019) · Food Science and Technology · doi:10.1590/fst.29818 · Cited by 35 · see citing works
Kumar, H., Schütz, F., Bhardwaj, K., Sharma, R., Nepovimová, E., Dhanjal, D. S., Verma, R., Kumar, D., Kuča, K., & Martins, N. A. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1996327 · Cited by 36 · see citing works
Yan, M., Wang, B., Xu, X., der Meister, T. J., Tabγač, H.-T., Hwang, F.-F., & Liu, Z. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02467 · Cited by 31 · see citing works
Lactobacilli-Derived Microbe-Associated Molecular Patterns (MAMPs) in Host Immune Modulation
Furnari, S., Ciantia, R., Garozzo, A., Furneri, P. M., & Fuochi, V. (2025) · Biomolecules · doi:10.3390/biom15111609 · Cited by 33 · see citing works
“Anti-inflammatory and anti-adhesion properties were also confirmed in ETEC-infected enterocytes [211].”
Kavitake, D., Tiwari, S., Devi, P. B., Shah, I. A., Reddy, G. B., & Shetty, P. H. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.129105 · Cited by 29 · see citing works
Microbiota and Probiotics: The Role of Limosilactobacillus Reuteri in Diverticulitis
Piccioni, A., Franza, L., Vaccaro, V., Saviano, A., Zanza, C., Candelli, M., Covino, M., Franceschi, F., & Ojetti, V. (2021) · Medicina · doi:10.3390/medicina57080802 · Cited by 28 · see citing works
“coli-induced pro-inflammatory cytokines such as interleukin (IL)-6 and IL-1b [42].”
BackgroundAnti-Helicobacter pylori Activity of a Lactobacillus sp. PW-7 Exopolysaccharide
Hu, J., Tian, X., Wei, T., Wu, H., Lü, J., Lyu, M., & Wang, S. (2021) · Foods · doi:10.3390/foods10102453 · Cited by 27 · see citing works
“As well as antibacterial activity, LAB EPS also possesses other biological activities, including antioxidant, anti-tumor, and anti-diabetic properties [20,26,27].”
BackgroundKiššová, Z., Tkáčiková, Ľ., Mudroňová, D., & Bhide, M. R. (2022) · Life · doi:10.3390/life12121955 · Cited by 27 · see citing works
“reuteri L26 for the purpose of the current experiment was performed exactly as described in our previous work [12].”
BackgroundMethodLimosilactobacillus reuteri Strains as Adjuvants in the Management of Helicobacter pylori Infection
Dargenio, C., Dargenio, V. N., Bizzoco, F., Indrio, F., Francavilla, R., & Cristofori, F. (2021) · Medicina · doi:10.3390/medicina57070733 · Cited by 26 · see citing works
Xu, W., Cao, F., Zhao, M., Fu, X., Yin, S., Sun, Y., Valencak, T. G., & Ren, D. (2022) · International Immunopharmacology · doi:10.1016/j.intimp.2022.108875 · Cited by 26 · see citing works
Vitlić, A., Sadiq, S., Ahmed, H. I., Ale, E. C., Binetti, A. G., Collett, A., Humpreys, P. N., & Laws, A. P. (2019) · Carbohydrate Research · doi:10.1016/j.carres.2019.03.003 · Cited by 24 · see citing works
Lactic Acid Bacteria—From Nature Through Food to Health
Teneva‐Angelova, T., Hristova, I., Pavlov, A. I., & Бешкова, Д. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-811443-8.00004-9 · Cited by 24 · see citing works
Meng, F., Lyu, Y., Zhao, H., Lyu, F., Bie, X., Lu, Y., Zhao, M., Chen, Y., & Lu, Z. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.11.180 · Cited by 20 · see citing works
Yang, J.-L., He, Y., Ai, Q., Liu, C., Ruan, Q.-Q., & Shi, Y. (2024) · Journal of Inflammation Research · doi:10.2147/jir.s459496 · Cited by 15 · see citing works
Karaffová, V., Csank, T., Mudroňová, D., Király, J., Révajová, V., Gancarčíková, S., Nemcová, R., Pistl, J., Vilček, Š., & Levkut, M. (2017) · Beneficial Microbes · doi:10.3920/bm2016.0114 · Cited by 18 · see citing works
Understanding Exopolysaccharides from Lactic Acid Bacteria: Synthesis, Functions, and Applications
Li, H., Jin, Y. J., Li, H., Zhao, J., Stanton, C., Ross, R. P., Chen, W., & Yang, B. K. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c04613 · Cited by 18 · see citing works
Ochôa, C., de Castro, F. C., Frias‐Bulhosa, J., Manso, M. C., Fernandes, J. C. H., & Fernandes, G. V. O. (2023) · Microorganisms · doi:10.3390/microorganisms11061449 · Cited by 21 · see citing works
“17938 produces exopolysaccharide, which benefits its adhesion to epithelial cells, competing with pathogenic bacteria for adhesion sites [49].”
BackgroundManoharan, D., Sivakumar, N., Billamboz, M., & Jawhara, S. (2024) · Applied Microbiology · doi:10.3390/applmicrobiol4010017 · Cited by 17 · see citing works
Polyphenols and Lactobacillus reuteri in oral health
Naureen, Z., Medori, M. C., Dhuli, K., Donato, K., Connelly, S., Bellinato, F., Gisondi, P., & Bertelli, M. (2022) · Journal of preventive medicine and hygiene · doi:10.15167/2421-4248/jpmh2022.63.2s3.2767 · Cited by 18 · see citing works
Ma, L., Xu, X., Peng, Q., Yang, S., Zhang, Y., Tian, D., Shi, L., Qiao, Y., & Shi, B. (2022) · Microbial Pathogenesis · doi:10.1016/j.micpath.2022.105863 · Cited by 15 · see citing works
Zhao, J., Fu, H., Zhang, Y., Li, M., Wang, D., Zhao, D., Zhang, J., & Wang, C. (2022) · Bioresources and Bioprocessing · doi:10.1186/s40643-022-00617-0 · Cited by 15 · see citing works
Dargenio, V. N., Cristofori, F., Dargenio, C., Giordano, P., Indrio, F., Celano, G., & Francavilla, R. (2022) · Beneficial Microbes · doi:10.3920/bm2021.0151 · Cited by 15 · see citing works
Oleksy, M., & Klewicka, E. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9295-5 · Cited by 13 · see citing works
“In addition, EPS have also been shown to exhibit cholesterollowering, anti-ulcer, antiviral activity and improvement of intestinal adhesion of probiotics [33, 34].”
BackgroundTkáčiková, Ľ., Mochnáčová, E., Tyagi, P., Kiššová, Z., & Bhide, M. R. (2020) · Veterinary Research · doi:10.1186/s13567-020-00773-1 · Cited by 13 · see citing works
Lim, H.-E., Song, Y.-B., Choi, H., & Lee, B.-H. (2024) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2024.121898 · Cited by 12 · see citing works
İnanan, T., Darilmaz, D. Ö., YEŞİLDAL, T. K., Yüksekdağ, Z., & Yavuz, S. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.136148 · Cited by 12 · see citing works
Gu, L.-X., Wen, Z., Xiang, X., Ma, L., Wang, X.-B., Jian-yin, M., & Qu, Y. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.03.008 · Cited by 12 · see citing works
Tang, S., Chuang, W., Liao, J., Tsai, Y., Chen, L., Liao, J., Ko, J., & Ou, C. (2025) · Food Science & Nutrition · doi:10.1002/fsn3.70318 · Cited by 11 · see citing works
“…reuteri is recognized as a significant producer of EPS, which has been reported to mitigate oxidative stress and inflammation by modulating immune responses and enhancing antioxidant enzyme activities including SOD, GPx, and CAT (Ferrer et al. 2024; Ksonzekova et al. 2016; Liu, Cheng, et al. 2024).”
Huo, P., Deng, L., Lu, J., Kan, P., Jing, R., & Luo, L.-J. (2025) · BMC Oral Health · doi:10.1186/s12903-025-06084-1 · Cited by 9 · see citing works
“7 cells [58].”
Lee, J., Chung, K., Shin, J., Jung, S.-H., Lee, S., Lee, M.-K., Hong, H., Rhee, Y. K., & Lee, K. (2022) · Polymers · doi:10.3390/polym14173594 · Cited by 9 · see citing works
“tacts with some gut bacteria such as Escherichia coli [36].”
BackgroundPadhi, S., Sharma, S., Sahoo, D., Montēt, D., & Rai, A. K. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-89875-1.00009-2 · Cited by 9 · see citing works
Liu, Z., Feng, L., Huang, Q., Liu, H., Liu, H., & Wan, H. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123791 · Cited by 9 · see citing works
Li, A., Yang, S., Han, X., Liu, C., Zheng, J., Ma, Y., Cheng, S., Zhao, J., Zhou, W., & Du, P. (2023) · Food Bioscience · doi:10.1016/j.fbio.2023.103437 · Cited by 8 · see citing works
Song, Y., Sun, M., Mu, G., & Tuo, Y. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.129478 · Cited by 8 · see citing works
Kahraman, M. S., Karahan, A. G., & Terzioğlu, M. E. (2022) · Current Microbiology · doi:10.1007/s00284-022-02915-4 · Cited by 8 · see citing works
“The antioxidative, immunostimulatory, and anticarcinogenic effects of EPS have been reported as well [41].”
BackgroundZhou, K., Xie, J., Su, Y., & Fang, J. (2023) · Biotechnology and Genetic Engineering Reviews · doi:10.1080/02648725.2023.2183617 · Cited by 8 · see citing works
“Furthermore, the exopolysaccharides secreted by LR can interfere with the adhesion and aggression of pathogenic bacteria, thus leading to decreased adhesion of enterotoxigenic Escherichia coli to intestinal epithelium cells (Kšonžeková et al., 2016; Schembri et al., 2004).”
Kober, A. K. M. H., Abdin, M., Subhash, A. J., Liu, S., Dertli, E., Abu‐Jdayil, B., Show, P. L., & Ayyash, M. M. (2025) · Food Chemistry X · doi:10.1016/j.fochx.2025.102164 · Cited by 7 · see citing works
Spangler, J. R., Caruana, J. C., Medintz, I. L., & Walper, S. A. (2021) · Future Science OA · doi:10.2144/fsoa-2020-0153 · Cited by 7 · see citing works
Erdem, T. K., Tatar, H. D., Ayman, S., & Gezginç, Y. (2023) · Turkish Journal of Agriculture - Food Science and Technology · doi:10.24925/turjaf.v11i2.414-423.5213 · Cited by 7 · see citing works
Kiššová, Z., Mudroňová, D., Link, R., & Tkáčiková, Ľ. (2023) · Veterinary Research Communications · doi:10.1007/s11259-023-10237-4 · Cited by 7 · see citing works
“In another of our studies, Kšonžeková et al. (2016) observed inhibition of ETEC adhesion to epithelial cells and subsequent reduction of gene expression of pro-inflammatory cytokines IL-1β and IL-6 induced by ETEC infection after pretreatment of IPEC-1 cells with EPS-L26 (Kšonžeková et al. 2016).”
BackgroundMethodResultInfluentialMudroňová, D., Karaffová, V., Csank, T., Király, J., Révajová, V., Gancarčíková, S., Nemcová, R., Pistl, J., Vilček, Š., & Levkut, M. (2018) · Beneficial Microbes · doi:10.3920/bm2017.0147 · Cited by 7 · see citing works
Sun, L., Liu, S., Ding, Y., Yang, R., & Zeng, H. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.105293 · Cited by 6 · see citing works
Treatment and prevention of pigeon diarrhea through the application of Lactobacillus SNK-6
Fan, W., Zhu, Y., Hou, H., Yao, J., Zhu, L., Liu, H., & Yan, H. (2024) · Poultry Science · doi:10.1016/j.psj.2024.103476 · Cited by 6 · see citing works
Lasaviciute, G., Plana, M. L., Örtegren, S. S., Telli, S., Kourmoulakis, S., Lundberg, L. E., Lidberg, K., Peiris, O., Sinha, I., Jonsson, A., et al. (2026) · Scientific Reports · doi:10.1038/s41598-026-45524-y · Cited by 5 · see citing works
“However, pro-inflammatory IL-1β was significantly induced by DSM 17938, while TNF-α and IL-6 expression were enhanced by the L26 strain 51 .”
Kiššová, Z., Štofilová, J., Mudroňová, D., & Karaffová, V. (2024) · Frontiers in Bioscience-Landmark · doi:10.31083/j.fbl2905180 · Cited by 5 · see citing works
Kiššová, Z., Schusterová, P., Mudroňová, D., Novotny, J. F., & Tkáčiková, Ľ. (2024) · Veterinary Research Communications · doi:10.1007/s11259-024-10445-6 · Cited by 5 · see citing works
“Relative normal - ized expression was calculated by the 2 − ΔΔCt method using CFX96 Manager software (Bio-Rad, Hercules, CA) with actin as the reference gene (Kšonžeková et al. 2016 ).”
BackgroundMethodInfluentialPostbiotics: Production to consumption as natural enhancers and preservatives in the food industry
Akhavan, F., Bari, M. R., Noori, S. M. A., & Amiri, S. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.108865 · Cited by 4 · see citing works
Limosilactobacillus reuteri DSM 17938 Produce Bioactive Components during Formulation in Sucrose
Lundberg, L. E., Forsberg, M. M., Lemanczyk, J., Sverremark‐Ekström, E., Sandström, C., Roos, S., & Håkansson, S. (2024) · Microorganisms · doi:10.3390/microorganisms12102058 · Cited by 4 · see citing works
“L. reuteri DSM 17938-derived EPS has been shown to inhibit the adhesion of enterotoxin-producing E. coli (ETEC) to intestinal IPEC-1 cells [9].”
BackgroundAli, S. K., Abdelgawad, M. A., Selim, S., Keshek, D. E.-G., Mohamed, H. S., & Hamed, E. A. (2025) · Current Microbiology · doi:10.1007/s00284-025-04300-3 · Cited by 4 · see citing works
Dong, Z., Li, X., Wu, Y., Wang, Z., Cui, W., Hu, S., Shi, D., Huang, Q., Xiao, Y., Zhou, H., et al. (2025) · Nutrients · doi:10.3390/nu17233667 · Cited by 4 · see citing works
Nieto, J. A., Rosés, C., Viadel, B., Gallego, E., Romo‐Hualde, A., Milagro, F. I., Barceló, A., Virto, R., Saldaña, G., & Luengo, E. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.132906 · Cited by 4 · see citing works
Argañaraz-Martínez, E., Apella, M. C., Chaia, A. P., & Babot, J. D. (2024) · Microbiome Research Reports · doi:10.20517/mrr.2025.64 · Cited by 3 · see citing works
Wang, D., Li, H., Zhao, Y. X., Cong, B., & Zhang, P. (2025) · Extremophiles · doi:10.1007/s00792-025-01394-2 · Cited by 3 · see citing works
“Polysaccha - ride with more branch from Lactobacillus reuteri induced stronger upregulation of inflammatory mediators than those with fewer branch (Ksonzeková et al. 2016).”
Exopolysaccharides May Increase Gastrointestinal Stress Tolerance of Lactobacillus reuteri
Fedorová, M., Nemcová, R., Mudroňová, D., Styková, E., Brudňáková, M., & Reiffová, K. (2018) · Folia veterinaria · doi:10.2478/fv-2018-0034 · Cited by 3 · see citing works
Ma, Y., Mao, Y., Deng, Z., Wang, S., Liu, J., & Wang, H. (2025) · Animal Nutriomics · doi:10.1017/anr.2025.3 · Cited by 2 · see citing works
“Previous studies found that L. reuteri produce lactic acid (Behare et al. 2023), reutericyclin (Huang et al. 2022) and exopolysaccharides (K ̌ son ̌ zeková et al. 2016), exhibiting antibacterial properties against certain intestinal pathogens in vitro (Al-Nabulsi et al. 2021; Bertin et al. 2017; K…”
Amani, A., Koroupi, K., Langarizadeh, M. A., Aghilinasab, A., Tavakoli, M. R., Afshari, A., & Forootanfar, H. (2025) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10786-x · Cited by 2 · see citing works
PROBIOTIC PROPERTIES OF LACTOBACILLUS REUTERI (L. REUTERI) STRAINS
Novikova, V. P., & Магомедова, Д. М. (2023) · Children s medicine of the North-West · doi:10.56871/cmn-w.2023.75.34.002 · Cited by 3 · see citing works
Wang, X., Chen, P., Wang, J., Wang, Y., Yu, M., Wang, X., Li, Q., Zhang, X., & Duan, J. (2023) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202300337 · Cited by 2 · see citing works
The MATH test. A three-phase assay?
Salas-Tovar, J. A., Escobedo‐García, S., Olivas, G. I., Acosta‐Muñiz, C. H., Harte, F., & Sepulveda, D. R. (2024) · FEMS Microbiology Letters · doi:10.1093/femsle/fnae045 · Cited by 2 · see citing works
Yalçın, S., Öztürk, Ş., & Keloğlu, B. (2018) · Nevşehir Bilim ve Teknoloji Dergisi · doi:10.17100/nevbiltek.402196 · Cited by 1 · see citing works
Schusterová, P., Mudroňová, D., Peňazziová, K., Hajdučková, V., & Csank, T. (2024) · Veterinární Medicína · doi:10.17221/106/2023-vetmed · Cited by 1 · see citing works
“…reuteri strain L26 Biocenol TM (CCM 8616) was originally isolated from the gut content of a healthy suckling piglet and its exopolysaccharide (EPS) was characterised as a high molecular weight d-glucan polysaccharide with (1 → 3) and (1 → 6) glycosidic linkages (Ksonzekova et al. 2016).”
InfluentialRay, M., Ashwini, M., Bhargava, N., & Mondal, K. C. (2025) · Springer eBooks · doi:10.1007/978-3-031-94472-7_14 · Cited by 1 · see citing works
Sitkin, S. I. (2026) · Almanac of Clinical Medicine · doi:10.18786/2072-0505-2026-54-001 · Cited by 1 · see citing works
Probiyotik Mayalar ve Özellikleri
YILDIRAN, H., Kılıç, G. B., & Karahan, A. G. (2017) · Turkish Journal of Agriculture - Food Science and Technology · doi:10.24925/turjaf.v5i10.1148-1155.1239 · Cited by 1 · see citing works
BEYAZ PEYNİRDEN İZOLE EDİLEN CRYPTOCOCCUS HUMICOLA SUŞLARININ STARTER AKTİVİTELERİNİN ARAŞTIRILMASI
UZUNDAĞ, D. (2020) · GIDA / THE JOURNAL OF FOOD · doi:10.15237/gida.gd20076 · Cited by 1 · see citing works
- Authors
- Kšonžeková, P., Bystrický, P., Vlčková, S., Pätoprstý, V., Pulzová, L., Mudroňová, D., Kubašková, T., Csank, T., & Tkáčiková, Ľ.
- Affiliations
- University of Veterinary Medicine in Košice (SK)
- Institute of Chemistry of the Slovak Academy of Sciences (SK)
- Corresponding author
- Tkáčiková, Ľudmila
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 141
- Pages
- 10-19
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2207148956
- Cited by
- 122
- References
- 38
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 22 · 2023: 14 · 2022: 18 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 2
- FWCI
- 10.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- VEGA (1/0834/12)
- Probiotech (26220220152)
- European Fund (OAE010101)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Lactobacillus reuteri, exopolysaccharides, enterotoxigenic Escherichia coli, bacterial adhesion, IPEC-1 cells, intestinal epithelial cells, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, NF-κB, glycosidic linkage, polysaccharide molecular weight, prebiotics
- MeSH terms
- Animals, Bacterial Adhesion, Cell Line, Epithelial Cells, Glucans, Polysaccharides, Bacterial, RNA, Messenger, Swine, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, NF-kappa B, Limosilactobacillus reuteri, Interleukin-1beta, Enterotoxigenic Escherichia coli
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review