Cited by 122Probiotics and Fermented FoodsPolysaccharides Composition and ApplicationsEscherichia coli research studies

Exopolysaccharides of Lactobacillus reuteri: Their Influence on Adherence of E. Coli to Epithelial Cells and Inflammatory Response

Kšonžeková, Petra, Bystrický, Peter, Vlčková, Silvia, Pätoprstý, Vladimír, Pulzová, Lucia, Mudroňová, Dagmar, Kubašková, Terézia, Csank, Tomáš +1 more·2016·Carbohydrate Polymers

Abstract

The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).

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Crossref · 10.1016/j.carbpol.2015.12.037

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  • High-performance anion-exchange chromatography coupled with pulsed amperometric detection or gas chromatography are commonly utilized to analyze the monosaccharide composition of EPS.

    “High-performance, anion-exchange chromatography coupled with pulsed amperometric detection [8,27,35,53] or gas chromatography (GC) [32,43] are commonly utilized to analyze the monosaccharide composition of EPS.”

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Authors
Kšonžeková, P., Bystrický, P., Vlčková, S., Pätoprstý, V., Pulzová, L., Mudroňová, D., Kubašková, T., Csank, T., & Tkáčiková, Ľ.
Affiliations
  • University of Veterinary Medicine in Košice (SK)
  • Institute of Chemistry of the Slovak Academy of Sciences (SK)
Corresponding author
Tkáčiková, Ľudmila
Venue
Carbohydrate Polymers
Publisher
Elsevier BV
Published
2016-05
Volume
141
Pages
10-19
ISSN
0144-8617 (ISSN-L)
OpenAlex
W2207148956
Cited by
122
References
38
Citations by year
2026: 9 · 2025: 16 · 2024: 22 · 2023: 14 · 2022: 18 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 2
FWCI
10.26
Type
journal article
Language
en
Open access
closed
Locations
  • Carbohydrate Polymers (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • VEGA (1/0834/12)
  • Probiotech (26220220152)
  • European Fund (OAE010101)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords
Lactobacillus reuteri, exopolysaccharides, enterotoxigenic Escherichia coli, bacterial adhesion, IPEC-1 cells, intestinal epithelial cells, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, NF-κB, glycosidic linkage, polysaccharide molecular weight, prebiotics
MeSH terms
Animals, Bacterial Adhesion, Cell Line, Epithelial Cells, Glucans, Polysaccharides, Bacterial, RNA, Messenger, Swine, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, NF-kappa B, Limosilactobacillus reuteri, Interleukin-1beta, Enterotoxigenic Escherichia coli

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