Cited by 447Genomics and Chromatin DynamicsCancer-related molecular mechanisms researchChromatin Remodeling and Cancer

RNA Binding to CBP Stimulates Histone Acetylation and Transcription

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Bose, Daniel A., Donahue, Greg, Reinberg, Danny, Shiekhattar, Ramin, Bonasio, Roberto, Berger, Shelley L.·2017·Cell

Abstract

CBP/p300 are transcription co-activators whose binding is a signature of enhancers, cis-regulatory elements that control patterns of gene expression in multicellular organisms. Active enhancers produce bi-directional enhancer RNAs (eRNAs) and display CBP/p300-dependent histone acetylation. Here, we demonstrate that CBP binds directly to RNAs in vivo and in vitro. RNAs bound to CBP in vivo include a large number of eRNAs. Using steady-state histone acetyltransferase (HAT) assays, we show that an RNA binding region in the HAT domain of CBP-a regulatory motif unique to CBP/p300-allows RNA to stimulate CBP's HAT activity. At enhancers where CBP interacts with eRNAs, stimulation manifests in RNA-dependent changes in the histone acetylation mediated by CBP, such as H3K27ac, and by corresponding changes in gene expression. By interacting directly with CBP, eRNAs contribute to the unique chromatin structure at active enhancers, which, in turn, is required for regulation of target genes.

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Crossref · 10.1016/j.cell.2016.12.020

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  • Enhancers are related to p300/CBP, as coactivators have been found as a component in the chromatin.

    “In this sense, enhancers have been related to p300/CBP, as coactivators have been found as a component in the chromatin.”
  • Specific expression profiles point toward increasing levels of monomethylation in the case of H3 and repressing levels of H3K4me3 marks.

    “Specific expression profiles have pointed towards increasing levels of monomethylation in the case of H3 and repressing levels of H3K4me3 marks.”

    From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [77]

    The STAR Methods section lists H3K4me1 and H3K4me3 datasets, but does not report increasing H3 monomethylation or repression of H3K4me3. The Results passages instead describe changes in histone acetylation. Searches for H3K4me1, H3K4me3, monomethylation, and expression profiles found no passage supporting the attributed claim; page numbers were unavailable in our copy. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Authors
Bose, D. A., Donahue, G., Reinberg, D., Shiekhattar, R., Bonasio, R., & Berger, S. L.
Affiliations
  • University of Pennsylvania (US)
  • New York University (US)
  • University of Miami (US)
Corresponding author
Berger, Shelley L.
Venue
Cell
Publisher
Elsevier BV
Published
2017-01
Volume
168
Issue
1-2
Pages
135-149.e22
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2578822114
Cited by
426
References
79
Citations by year
2026: 25 · 2025: 32 · 2024: 55 · 2023: 38 · 2022: 51 · 2021: 66 · 2020: 57 · 2019: 37 · 2018: 42 · 2017: 22
FWCI
17.88
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (R01 CA078831)
  • NIH Innovator Award (DP2MH107055)
  • Searle Scholars Program (15-SSP-102)
  • March of Dimes Foundation (1-FY-15-344)
  • W.W. Smith Charitable Trust (C1404)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
Keywords
CBP, P300, RNA binding, enhancer RNA, histone acetylation, histone acetyltransferase activity, H3K27ac, transcriptional coactivator, gene expression regulation
MeSH terms
Acetylation, Animals, Cell Line, Enhancer Elements, Genetic, Fibroblasts, Histones, RNA, Untranslated, p300-CBP Transcription Factors, Mice, Histone Acetyltransferases

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