The DOI matches its Crossref record; 78 of the 78 references it cites were found in a registry or an index.
Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture
Open accessAbstract
In the healthy adult liver, most hepatocytes proliferate minimally. However, upon physical or chemical injury to the liver, hepatocytes proliferate extensively in vivo under the direction of multiple extracellular cues, including Wnt and pro-inflammatory signals. Currently, liver organoids can be generated readily in vitro from bile-duct epithelial cells, but not hepatocytes. Here, we show that TNFα, an injury-induced inflammatory cytokine, promotes the expansion of hepatocytes in 3D culture and enables serial passaging and long-term culture for more than 6 months. Single-cell RNA sequencing reveals broad expression of hepatocyte markers. Strikingly, in vitro-expanded hepatocytes engrafted, and significantly repopulated, the injured livers of Fah-/- mice. We anticipate that tissue repair signals can be harnessed to promote the expansion of otherwise hard-to-culture cell-types, with broad implications.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Organoids of murine intestines31, livers35,125,126 and pancreas69,146 were successfully transplanted into mice, and the transplanted organoids could restore organ dysfunction.”
From Organoids (2022) · cites this paper as [125]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In the case of the liver, both cholangiocyte (ductal cell of the bile duct)35,36 and hepatocyte organoids125,126 have been established, both from mouse and human tissue samples.”
From Organoids (2022) · cites this paper as [125]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In the example of hepatocyte organoids125, hepatocyte functions are preserved, yet the liver tissue architecture does not match the native tissue where hepatocytes are arranged in cords.”
From Organoids (2022) · cites this paper as [125]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Several of the most dramatic expansion results reported to date—including near-unlimited theoretical fold-expansion and multi-month clonal proliferation—have been demonstrated almost exclusively in mouse hepatocytes [18,31], whereas human systems have generally achieved more modest expansion with measurable trade-offs in redifferentiation and repopulation capacity at higher expansion levels [4,17]”
From The Hepatocyte Expansion Paradox: A Review of In Vitro Challenges and Advances (2026) · cites this paper as [31]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Compact 3D liver models enhanced with tumor necrosis factor-α (TNFα) have demonstrated the capacity to sustain long-term hepatocyte culture, encompassing serial passaging and expansion over several months, and thus illustrate the importance of precisely regulated inflammatory signaling in facilitating hepatocyte plasticity and viability [31].”
From The Hepatocyte Expansion Paradox: A Review of In Vitro Challenges and Advances (2026) · cites this paper as [31]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Gene Ontology: tool for the unification of biology
Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., et al. (2000) · Nature Genetics · doi:10.1038/75556 · Cited by 43,206
Integrating single-cell transcriptomic data across different conditions, technologies, and species
Butler, A., Hoffman, P., Smibert, P., Papalexi, E., & Satija, R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4096 · Cited by 13,174
Massively parallel digital transcriptional profiling of single cells
Zheng, G. X. Y., Terry, J. M., Belgrader, P., Ryvkin, P., Bent, Z. W., Wilson, R., Ziraldo, S. B., Wheeler, T. D., McDermott, G. P., Zhu, J., et al. (2017) · Nature Communications · doi:10.1038/ncomms14049 · Cited by 7,353
Self-citationOrganoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,519
A global double-fluorescent Cre reporter mouse
Muzumdar, M. D., Tasic, B., Miyamichi, K., Li, L., & Luo, L. (2007) · genesis · doi:10.1002/dvg.20335 · Cited by 3,491
NF-κB: linking inflammation and immunity to cancer development and progression
Karin, M., & Greten, F. R. (2005) · Nature reviews. Immunology · doi:10.1038/nri1703 · Cited by 3,175
Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
The Tabula Muris Consortium, coordination, O., coordination, L., processing, O. C. A., sequencing, L. P. A., analysis, C. D., annotation, C. T., Writing group, Supplemental text writing group, & Principal investigators (2018) · Nature · doi:10.1038/s41586-018-0590-4 · Cited by 2,842
Michalopoulos, G. K., & DeFrances, M. C. (1997) · Science · doi:10.1126/science.276.5309.60 · Cited by 2,828
Edmondson, R., Broglie, J. J., Adcock, A. F., & Yang, L. (2014) · ASSAY and Drug Development Technologies · doi:10.1089/adt.2014.573 · Cited by 2,515
NF-κB functions as a tumour promoter in inflammation-associated cancer
Pikarsky, E., Porat, R. M., Stein, I., Abramovitch, R., Amit, S., Kasem, S., Gutkovich-Pyest, E., Urieli-Shoval, S., Galun, E., & Ben‐Neriah, Y. (2004) · Nature · doi:10.1038/nature02924 · Cited by 2,423
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170
The economics of ribosome biosynthesis in yeast
Warner, J. R. (1999) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(99)01460-7 · Cited by 1,933
Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-κB
Beg, A. A., Sha, W. C., Bronson, R. T., Ghosh, S., & Baltimore, D. (1995) · Nature · doi:10.1038/376167a0 · Cited by 1,828
Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
Huch, M., Gehart, H., van Boxtel, R., Hamer, K., Blokzijl, F., Verstegen, M. M. A., Ellis, E., van Wenum, M., Fuchs, S. A., de Ligt, J., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.050 · Cited by 1,619
Liver regeneration: from myth to mechanism
Taub, R. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1489 · Cited by 1,567
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Huch, M., Dorrell, C., Boj, S. F., van Es, J. H., Li, V. S. W., van de Wetering, M., Sato, T., Hamer, K., Sasaki, N., Finegold, M. J., et al. (2013) · Nature · doi:10.1038/nature11826 · Cited by 1,555
Self-citationLiver Failure and Defective Hepatocyte Regeneration in Interleukin-6-Deficient Mice
Cressman, D. E., Greenbaum, L. E., DeAngelis, R. A., Ciliberto, G., Furth, E. E., Poli, V., & Taub, R. (1996) · Science · doi:10.1126/science.274.5291.1379 · Cited by 1,538
Human primary liver cancer–derived organoid cultures for disease modeling and drug screening
Broutier, L., Mastrogiovanni, G., Verstegen, M. M., Francies, H. E., Gavarró, L. M., Bradshaw, C. R., Allen, G. E., Arnes-Benito, R., Sidorova, O., Gaspersz, M. P., et al. (2017) · Nature Medicine · doi:10.1038/nm.4438 · Cited by 1,471
An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control
Clevers, H. C., Loh, K. M., & Nusse, R. (2014) · Science · doi:10.1126/science.1248012 · Cited by 1,386
Self-citationMichalopoulos, G. K. (2007) · Journal of Cellular Physiology · doi:10.1002/jcp.21172 · Cited by 1,384
Liver immunology and its role in inflammation and homeostasis
Robinson, M. W., Harmon, C., & O’Farrelly, C. (2016) · Cellular & Molecular Immunology · doi:10.1038/cmi.2016.3 · Cited by 1,151
Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration
Lindemans, C. A., Calafiore, M., Mertelsmann, A. M., O'Connor, M. H., Dudakov, J. A., Jenq, R. R., Velardi, E., Young, L. F., Smith, O. M., Lawrence, G., et al. (2015) · Nature · doi:10.1038/nature16460 · Cited by 1,144
Intestinal Tumorigenesis Initiated by Dedifferentiation and Acquisition of Stem-Cell-like Properties
Schwitalla, S., Fingerle, A. A., Cammareri, P., Nebelsiek, T., Göktuna, S. İ., Ziegler, P. K., Canli, Ö., Heijmans, J., Huels, D. J., Moreaux, G., et al. (2012) · Cell · doi:10.1016/j.cell.2012.12.012 · Cited by 1,098
Single-cell spatial reconstruction reveals global division of labour in the mammalian liver
Halpern, K. B., Shenhav, R., Matcovitch-Natan, O., Tóth, B., Lemze, D., Golan, M., Massasa, E. E., Baydatch, S., Landen, S., Moor, A. E., et al. (2017) · Nature · doi:10.1038/nature21065 · Cited by 1,059
TNFα promotes proliferation of oligodendrocyte progenitors and remyelination
Arnett, H. A., Mason, J., Marino, M., Suzuki, K., Matsushima, G. K., & Ting, J. P.-Y. (2001) · Nature Neuroscience · doi:10.1038/nn738 · Cited by 1,012
Yamada, Y., Kirillova, I., Peschon, J. J., & Fausto, N. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.4.1441 · Cited by 944
Hippo Pathway Activity Influences Liver Cell Fate
Yimlamai, D., Christodoulou, C., Galli, G. G., Yanger, K., Pepe-Mooney, B., Gurung, B., Shrestha, K., Cahan, P., Stanger, B. Z., & Camargo, F. D. (2014) · Cell · doi:10.1016/j.cell.2014.03.060 · Cited by 857
Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids
Hu, H., Gehart, H., Artegiani, B., LÖpez-Iglesias, C., Dekkers, F., Basak, O., van Es, J., Chuva de Sousa Lopes, S. M., Begthel, H., Korving, J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.013 · Cited by 837
Robust expansion of human hepatocytes in Fah−/−/Rag2−/−/Il2rg−/− mice
Azuma, H., Paulk, N., Ranade, A., Dorrell, C., Al-Dhalimy, M., Ellis, E., Strom, S., Kay, M. A., Finegold, M., & Grompe, M. (2007) · Nature Biotechnology · doi:10.1038/nbt1326 · Cited by 835
Self-citationBroutier, L., Andersson-Rolf, A., Hindley, C. J., Boj, S. F., Clevers, H., Koo, B.-K., & Huch, M. (2016) · Nature Protocols · doi:10.1038/nprot.2016.097 · Cited by 834
Reparative inflammation takes charge of tissue regeneration
Karin, M., & Clevers, H. (2016) · Nature · doi:10.1038/nature17039 · Cited by 767
Self-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver
Wang, B., Zhao, L., Fish, M., Logan, C. Y., & Nusse, R. (2015) · Nature · doi:10.1038/nature14863 · Cited by 674
Self-citationThe R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength
de Lau, W., Peng, W. C., Gros, P., & Clevers, H. (2014) · Genes & Development · doi:10.1101/gad.235473.113 · Cited by 656
Self-citationMultilineage communication regulates human liver bud development from pluripotency
Camp, J. G., Sekine, K., Gerber, T., Loeffler-Wirth, H., Binder, H., Gac, M., Kanton, S., Kageyama, J., Damm, G., Seehofer, D., et al. (2017) · Nature · doi:10.1038/nature22796 · Cited by 621
Modeling mouse and human development using organoid cultures
Huch, M., & Koo, B. (2015) · Development · doi:10.1242/dev.118570 · Cited by 578
Stem/progenitor cells in liver development, homeostasis, regeneration, and reprogramming
Miyajima, A., Tanaka, M., & Itoh, T. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.04.010 · Cited by 570
Overturf, K., Al-Dhalimy, M., Tanguay, R., Brantly, M., Ou, C.-N., Finegold, M., & Grompe, M. (1996) · Nature Genetics · doi:10.1038/ng0396-266 · Cited by 549
Self-citationApc Tumor Suppressor Gene Is the “Zonation-Keeper” of Mouse Liver
Benhamouche, S., Decaens, T., Godard, C., Chambrey, R., Rickman, D. S., Moinard, C., Vasseur-Cognet, M., Kuo, C. J., Kahn, A., Perret, C., et al. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.03.015 · Cited by 543
Metabolic zonation of the liver: The oxygen gradient revisited
Kietzmann, T. (2017) · Redox Biology · doi:10.1016/j.redox.2017.01.012 · Cited by 499
van Amerongen, R., Bowman, A. N., & Nusse, R. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.05.023 · Cited by 454
Self-citationHepatostat: Liver regeneration and normal liver tissue maintenance
Michalopoulos, G. K. (2017) · Hepatology · doi:10.1002/hep.28988 · Cited by 444
Gerstenfeld, L. C., Cho, T.-J., Kon, T., Aizawa, T., Tsay, A., Fitch, J. L., Barnes, G. B., Graves, D. T., & Einhorn, T. A. (2003) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2003.18.9.1584 · Cited by 434
Transcription Factors in Liver Development, Differentiation, and Regeneration
Costa, R. H., Kalinichenko, V. V., Holterman, A. L., & Wang, X. (2003) · Hepatology · doi:10.1016/j.hep.2003.09.034 · Cited by 408
Antibodies to tumor necrosis factor-alpha inhibit liver regeneration after partial hepatectomy
Akerman, P., Cote, P., Yang, S. Q., McClain, C., Nelson, S., Bagby, G. J., & Diehl, A. M. (1992) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1992.263.4.g579 · Cited by 401
Lindroos, P. M., Zarnegar, R., & Michalopoulos, G. K. (1991) · Hepatology · doi:10.1002/hep.1840130422 · Cited by 385
Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Zhang, R., Koike, H., Kimura, M., Yoshizawa, E., Enomura, M., Koike, N., Sekine, K., & Taniguchi, H. (2014) · Nature Protocols · doi:10.1038/nprot.2014.020 · Cited by 366
The RSPO–LGR4/5–ZNRF3/RNF43 module controls liver zonation and size
Planas-Paz, L., Orsini, V., Boulter, L., Calabrese, D., Pikiolek, M., Nigsch, F., Xie, Y., Roma, G., Donovan, A., Marti, P., et al. (2016) · Nature Cell Biology · doi:10.1038/ncb3337 · Cited by 359
Grompe, M., al-Dhalimy, M., Finegold, M., Ou, C. N., Burlingame, T., Kennaway, N. G., & Soriano, P. (1993) · Genes & Development · doi:10.1101/gad.7.12a.2298 · Cited by 355
Self-citationParasitic helminths induce fetal-like reversion in the intestinal stem cell niche
Nusse, Y. M., Savage, A. K., Marangoni, P., Huber, A. R., Landman, T. A., de Sauvage, F. J., Locksley, R. M., & Klein, O. D. (2018) · Nature · doi:10.1038/s41586-018-0257-1 · Cited by 339
Immune Modulation of Stem Cells and Regeneration
Aurora, A. B., & Olson, E. N. (2014) · Cell stem cell · doi:10.1016/j.stem.2014.06.009 · Cited by 322
Oguma, K., Oshima, H., Aoki, M., Uchio, R., Naka, K., Nakamura, S., Hirao, A., Saya, H., Taketo, M. M., & Oshima, M. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.105 · Cited by 287
Tumor Necrosis Factor Primes Hepatocytes for Dna Replication in the Rat
Webber, E. M., Bruix, J., Pierce, R. H., & Fausto, N. (1998) · Hepatology · doi:10.1002/hep.510280509 · Cited by 280
Yamada, Y., Webber, E. M., Kirillova, I., Peschon, J. J., & Fausto, N. (1998) · Hepatology · doi:10.1002/hep.510280410 · Cited by 260
Katsuda, T., Kawamata, M., Hagiwara, K., Takahashi, R.-U., Yamamoto, Y., Camargo, F. D., & Ochiya, T. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2016.10.007 · Cited by 260
Organ-Level Quorum Sensing Directs Regeneration in Hair Stem Cell Populations
Chen, C., Wang, L., Plikus, M. V., Jiang, T. X., Murray, P. J., Ramos, R., Guerrero‐Juarez, C. F., Hughes, M. W., Lee, O. K., Shi, S., et al. (2015) · Cell · doi:10.1016/j.cell.2015.02.016 · Cited by 245
Distributed hepatocytes expressing telomerase repopulate the liver in homeostasis and injury
Lin, S., Nascimento, E. M., Gajera, C. R., Chen, L., Neuhöfer, P., Garbuzov, A., Wang, S., & Artandi, S. E. (2018) · Nature · doi:10.1038/s41586-018-0004-7 · Cited by 241
Ramadori, G. P., & Armbrust, T. (2001) · European Journal of Gastroenterology & Hepatology · doi:10.1097/00042737-200107000-00004 · Cited by 231
Wullaert, A., Loo, G., Heyninck, K., & Beyaert, R. (2007) · Endocrine Reviews · doi:10.1210/er.2006-0031 · Cited by 221
Taniguchi, E., Sakisaka, S., Matsuo, K., Tanikawa, K., & Sata, M. (2001) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540104900112 · Cited by 216
The Angiocrine Factor Rspondin3 Is a Key Determinant of Liver Zonation
Rocha, A. S., Vidal, V., Mertz, M., Kendall, T. J., Charlet, A., Okamoto, H., & Schedl, A. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.10.049 · Cited by 194
Shimizu, H., Miyazaki, M., Wakabayashi, Y., Mitsuhashi, N., Kato, A., Ito, H., Nakagawa, K., Yoshidome, H., Kataoka, M., & Nakajima, N. (2001) · Journal of Hepatology · doi:10.1016/s0168-8278(00)00055-6 · Cited by 161
Influence of epidermal growth factor on liver regeneration after partial hepatectomy in rats
Olsen, P. S., Boesby, S., Kirkegaard, P. O., Therkelsen, K., Almdal, T., Poulsen, S. S., & Nexø, E. (1988) · Hepatology · doi:10.1002/hep.1840080503 · Cited by 151
Zonal gene expression in murine liver: Lessons from tumors
Hailfinger, S., Jaworski, M., Braeuning, A., Buchmann, A., & Schwarz, M. (2006) · Hepatology · doi:10.1002/hep.21082 · Cited by 149
Kirillova, I., Chaisson, M. L., & Fausto, N. (1999) · PubMed · Cited by 143
Signals and Cells Involved in Regulating Liver Regeneration
Kang, L., Mars, W. M., & Michalopoulos, G. K. (2012) · Cells · doi:10.3390/cells1041261 · Cited by 137
Borkham‐Kamphorst, E., de Leur, E. V., Zimmermann, H. W., Karlmark, K. R., Tihaa, L., Haas, U., Tacke, F., Berger, T., Mak, T. W., & Weiskirchen, R. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2013.01.014 · Cited by 135
Michalopoulos, G. K., Bowen, W. C., Mulé, K., & Luo, J. (2003) · Gene Expression · doi:10.3727/000000003108748964 · Cited by 135
Preziosi, M., Okabe, H., Poddar, M., Singh, S., & Monga, S. P. (2018) · Hepatology Communications · doi:10.1002/hep4.1196 · Cited by 135
Cell proliferation and renewal of normal hepatocytes and bile duct cells in adult mouse liver
Magami, Y., Azuma, T., Inokuchi, H., Kokuno, S., Moriyasu, F., Kawai, K., & Hattori, T. (2002) · Liver International · doi:10.1034/j.1600-0676.2002.01702.x · Cited by 124
IL-6 Signaling Regulates Small Intestinal Crypt Homeostasis
Jeffery, V., Goldson, A. J., Dainty, J. R., Chieppa, M., & Sobolewski, A. (2017) · The Journal of Immunology · doi:10.4049/jimmunol.1600960 · Cited by 107
STAT-3 Overexpression and p21 Up-Regulation Accompany Impaired Regeneration of Fatty Livers
Torbenson, M. S., Yang, S. Q., Liu, H. Z., Huang, J., Gage, W. R., & Diehl, A. M. (2002) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64167-3 · Cited by 97
Lee, J., Homma, T., Kurahashi, T., Kang, E. S., & Fujii, J. (2015) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2015.06.121 · Cited by 95
Adult Mouse Liver Contains Two Distinct Populations of Cholangiocytes
Li, B., Dorrell, C., Canaday, P. S., Pelz, C., Haft, A., Finegold, M. J., & Grompe, M. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.06.003 · Cited by 69
Self-citationTranscription factors in liver development, differentiation, and regeneration
Costa, R. M. M. (2003) · Hepatology · doi:10.1053/jhep.2003.09034 · Cited by 67
Two-signal requirement for growth-promoting function of Yap in hepatocytes
Su, T., Bondar, T., Zhou, X., Zhang, C., He, H., & Medzhitov, R. (2015) · eLife · doi:10.7554/elife.02948 · Cited by 64
Impaired neural development caused by inducible expression of Axin in transgenic mice
Yu, H. I., Liu, B., Costantini, F. D., & Hsu, W. (2006) · Mechanisms of Development · doi:10.1016/j.mod.2006.10.002 · Cited by 60
Shimizu, H. (2005) · World Journal of Gastroenterology · doi:10.3748/wjg.v11.i46.7254 · Cited by 34
Lipocalin 2 Upregulation Protects Hepatocytes from IL1-β-Induced Stress
Hu, Y., Xue, J., Yang, Y., Zhou, X., Qin, C., Zheng, M., Zhu, H., Liu, Y., Liu, W., Lou, G., et al. (2015) · Cellular Physiology and Biochemistry · doi:10.1159/000430135 · Cited by 21
Flagged first, then by citations
Cited by
Sorted by citations
Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184
Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,082
Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids
Hu, H., Gehart, H., Artegiani, B., LÖpez-Iglesias, C., Dekkers, F., Basak, O., van Es, J., Chuva de Sousa Lopes, S. M., Begthel, H., Korving, J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.013 · Cited by 837
The immunological and metabolic landscape in primary and metastatic liver cancer
Li, X., Ramadori, P., Pfister, D. R., Seehawer, M., Zender, L., & Heikenwälder, M. F. (2021) · Nature reviews. Cancer · doi:10.1038/s41568-021-00383-9 · Cited by 639
Corrò, C., Novellasdemunt, L., & Li, V. S. W. (2020) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00120.2020 · Cited by 561
Metadata
- Authors
- Peng, W. C., Logan, C. Y., Fish, M., Anbarchian, T., Aguisanda, F., Álvarez-Varela, A., Wu, P., Jin, Y., Zhu, J., Li, B., Grompe, M., Wang, B., & Nusse, R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Stanford University (US)
- Oregon Health & Science University (US)
- University of California, San Francisco (US)
- Corresponding author
- Peng, Weng Chuan
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 175
- Issue
- 6
- Pages
- 1607-1619.e15
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC6497386
- OpenAlex
- W2902626180
- Cited by
- 331
- References
- 78
- Citations by year
- 2026: 33 · 2025: 43 · 2024: 48 · 2023: 40 · 2022: 49 · 2021: 59 · 2020: 39 · 2019: 19 · 2018: 1
- FWCI
- 18.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- California Institute for Regenerative Medicine
- NIH (S10OD020141)
- Stanford Child Health Research Institute
- Burroughs Wellcome Fund
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- TNF-α, hepatocyte expansion, primary hepatocytes, 3D cell culture, liver regeneration, hepatocyte engraftment, liver injury, hepatocyte markers, long-term culture
- MeSH terms
- Animals, Antigens, Differentiation, Cell Line, Transformed, Humans, Liver, Time Factors, Tumor Necrosis Factor-alpha, Mice, Knockout, Spheroids, Cellular, Cell Culture Techniques, Hepatocytes, Cell Proliferation, Hep G2 Cells, Human Umbilical Vein Endothelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
