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Cited by 5613D Printing in Biomedical ResearchCancer Cells and MetastasisPluripotent Stem Cells Research

A brief history of organoids

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Corrò, Claudia, Novellasdemunt, Laura, Li, Vivian S.W.·2020·American Journal of Physiology-Cell Physiology
DOI 10.1152/ajpcell.00120.2020PMC PMC7468890
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Abstract

In vitro cell cultures are crucial research tools for modeling human development and diseases. Although the conventional monolayer cell cultures have been widely used in the past, the lack of tissue architecture and complexity of such model fails to inform the true biological processes in vivo. Recent advances in the organoid technology have revolutionized the in vitro culture tools for biomedical research by creating powerful three-dimensional (3D) models to recapitulate the cellular heterogeneity, structure, and functions of the primary tissues. Such organoid technology enables researchers to recreate human organs and diseases in a dish and thus holds great promises for many translational applications such as regenerative medicine, drug discovery, and precision medicine. In this review, we provide an overview of the organoid history and development. We discuss the strengths and limitations of organoids as well as their potential applications in the laboratory and the clinic.

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  • The presence of abnormal cell states and altered differentiation patterns in organoid cultures have been observed in a variety of tissues, including skin, using more conventional methods.

    “The presence of abnormal cell states and altered differentiation patterns in organoid cultures have been observed in a variety of tissues,4 including skin,9 using more conventional methods.”

    From Single-cell transcriptomics of human-skin-equivalent organoids (2023) · cites this paper as [4]

    The section ‘Strengths and Limitations of 3D Organoid Cultures Over Conventional Models’ (page unavailable) reports abnormal cellular stress and impaired cell-type specification in cortical organoids. It does not establish that these changes were observed across a variety of tissues or specify the conventional methods claimed. The skin example is separately cited in the citing sentence, so it is not attributed to this source. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Matrigel is used in the majority of organoid systems.

    “Matrigel is used in the majority of organoid systems4”

    From Single-cell transcriptomics of human-skin-equivalent organoids (2023) · cites this paper as [4]

    In “Other Organoid Types” (page not provided), the review says the vast majority of adult stem cell–derived organoids are cultured in Matrigel. The “3D Culture Models” section also calls Matrigel the most commonly used scaffold. Neither passage establishes that a majority of all organoid systems use it, so the claim extends beyond the stated scope. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Corrò, C., Novellasdemunt, L., & Li, V. S. W.
Affiliations
The Francis Crick Institute (GB)
Corresponding author
Li, Vivian S.W.
ORCID
Venue
American Journal of Physiology-Cell Physiology
Publisher
American Physiological Society
Published
2020-07-01
Volume
319
Issue
1
Pages
C151-C165
ISSN
  • 0363-6143 (ISSN-L)
  • 1522-1563
DOI
10.1152/ajpcell.00120.2020
PMC
PMC7468890
OpenAlex
W3028914388
Cited by
561
References
41
Citations by year
2026: 84 · 2025: 134 · 2024: 163 · 2023: 78 · 2022: 63 · 2021: 36 · 2020: 3
FWCI
3.76
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • American Journal of Physiology-Cell Physiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
  • Wellcome Trust (FC001105)
  • Francis Crick Institute (10105)
  • Cancer Research UK (FC001105)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
organoids, 3D cell culture, in vitro tissue models, disease modeling, regenerative medicine, drug discovery, precision medicine, tissue structure
MeSH terms
Animals, Humans, Models, Biological, Organ Culture Techniques, Organoids, Cell Culture Techniques, Biomedical Research

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