Cited by 62Paleopathology and ancient diseasesArchaeology and ancient environmental studiesGut microbiota and health

Hallstatt miners consumed blue cheese and beer during the Iron Age and retained a non-Westernized gut microbiome until the Baroque period

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Maixner, Frank, Sarhan, Mohamed S., Huang, Kun D., Tett, Adrian, Schoenafinger, Alexander, Zingale, Stefania, Blanco-Míguez, Aitor, Manghi, Paolo +13 more·2021·Current Biology

Abstract

century AD) to in-depth microscopic, metagenomic, and proteomic analyses. The paleofeces were preserved in the underground salt mines of the UNESCO World Heritage site of Hallstatt in Austria. This allowed us to reconstruct the diet of the former population and gain insights into their ancient gut microbiome composition. Our dietary survey identified bran and glumes of different cereals as some of the most prevalent plant fragments. This highly fibrous, carbohydrate-rich diet was supplemented with proteins from broad beans and occasionally with fruits, nuts, or animal food products. Due to these traditional dietary habits, all ancient miners up to the Baroque period have gut microbiome structures akin to modern non-Westernized individuals whose diets are also mainly composed of unprocessed foods and fresh fruits and vegetables. This may indicate a shift in the gut community composition of modern Westernized populations due to quite recent dietary and lifestyle changes. When we extended our microbial survey to fungi present in the paleofeces, in one of the Iron Age samples, we observed a high abundance of Penicillium roqueforti and Saccharomyces cerevisiae DNA. Genome-wide analysis indicates that both fungi were involved in food fermentation and provides the first molecular evidence for blue cheese and beer consumption in Iron Age Europe.

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  • Strains from other alcoholic beverages (e.g., spirits) were less clustered, in agreement with previous studies.

    “Strains from other alcoholic beverages (e.g., spirits) were less clustered, in agreement with previous studies. 115,121”

    From Unexplored microbial diversity from 2,500 food metagenomes and links with the human microbiome. (2024) · cites this paper as [115]

    The Results (Molecular evidence for blue cheese and beer consumption during the Iron Age; page unavailable) place spirit strains in a mixed clade alongside bread, wine, and beer strains, supporting the example of spirits as less distinctly clustered. But the paper also identifies a separate wine clade, so it does not support the broader claim that strains from other alcoholic beverages were generally less clustered. (LLM, conf=0.82)

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  1. [22]Grant W.D. Halococcus Trujillo M.E. Dedysh S. DeVos P. Hedlund B. Kämpfer P. Rainey F.A. Whitman W.B. Bergey’s Manual of Systematics of Archaea and Bacteria 2015 John Wiley and Sons
  2. [56]Cantor M.D. van den Tempel T. Hansen T.K. Ardö Y. Blue cheese McSweeney P.L.H. Fox P.F. Cotter P.D. Everett D.W. Cheese, 2017 Elsevier 929 954
  3. [58]Desmasures N. Mold-ripened varieties Batt C. Encyclopedia of Food Microbiology 2014 Elsevier 409 416

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Authors
Maixner, F., Sarhan, M. S., Huang, K. D., Tett, A., Schoenafinger, A., Zingale, S., Blanco-Míguez, A., Manghi, P., Cemper-Kiesslich, J., Rosendahl, W., Kusebauch, U., Morrone, S. R., Hoopmann, M. R., Rota-Stabelli, O., Rattei, T., Moritz, R. L., Oeggl, K., Segata, N., Zink, A., Reschreiter, H., & Kowarik, K.
Affiliations
  • Eurac Research (IT)
  • Fondazione Edmund Mach (IT)
  • University of Vienna (AT)
  • University of Trento (IT)
  • Reiss Engelhorn Museum (DE)
  • Institute for Systems Biology (US)
  • Universität Innsbruck (AT)
  • Natural History Museum Vienna (AT)
Corresponding author
  • Maixner, Frank
  • Kowarik, Kerstin
Venue
Current Biology
Publisher
Elsevier BV
Published
2021-12
Volume
31
Issue
23
Pages
5149-5162.e6
ISSN
0960-9822 (ISSN-L)
OpenAlex
W3206940038
Cited by
62
References
125
Citations by year
2026: 12 · 2025: 14 · 2024: 18 · 2023: 11 · 2022: 6 · 2021: 1
FWCI
44.30
Type
journal article
Language
en
Open access
Locations
  • Current Biology (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Institutional Research Information System (Università degli Studi di Trento) (submittedVersion, cc-by-nc-nd)
  • CINECA IRIS Institutional Research Information System (Fondazione Edmund Mach) (submittedVersion, cc-by-nc-nd)
  • Open Research (University of Surrey) (submittedVersion, cc-by-nc-nd)
Funders
  • National Science Foundation (1920268)
  • Provincia autonoma di Bolzano - Alto Adige (FESR1078-MummyLabs)
  • National Institutes of Health (1s10od026936-01, GM087221)
  • European Regional Development Fund (2014-2020_CALL-FESR 2017, FESR1078-MummyLabs)
  • National Institute of General Medical Sciences (R01GM087221)
Topics
  • Social Sciences › Arts and Humanities › Archeology › Paleopathology and ancient diseases
  • Physical Sciences › Earth and Planetary Sciences › Paleontology › Archaeology and ancient environmental studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
paleofeces, salt mines, blue cheese, beer, Penicillium roqueforti, Saccharomyces cerevisiae, food fermentation, cereals, Vicia faba, molecular archaeology
MeSH terms
Gastrointestinal Microbiome, Animals, Beer, Cheese, Diet, Fungi, Proteomics

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