Identification and functional analysis of TopBP1 and its homologs
Open accessAbstract
The multiple BRCT-domain protein TopBP1 and its yeast homologs have been implicated in many aspects of DNA metabolism, but their molecular functions remain elusive. In this review, we first summarise how the yeast homologs were identified and characterised. We next review the data available from metazoan systems and finally draw parallels with the yeast models. TopBP1 plays important functions in the initiation of DNA replication in all organisms and participates in checkpoint responses both within S phase and following DNA damage. In metazoan systems there is accumulating evidence for additional roles in transcriptional regulation that have not been reported in yeast. Overall, TopBP1 appears to play a key role in integrating different aspects of DNA metabolism, but the mechanistic basis for this remains to be fully explained.
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The ATR-ATRIP complex and the 9-1-1 complex are recruited to RPA-coated single-stranded DNA independently and are bridged by TopBP1.
“The ATR-ATRIP complex and 9-1-1 complexes are recruited to RPA-ssDNA independently and are bridged by the multiple-function protein TopBP1 (Topoisomerase IIβ binding protein 1) [106–110].”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [109]
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Karppinen, S.-M., Erkko, H., Reini, K., Pospiech, H., Heikkinen, K., Rapakko, K., Syväoja, J. E., & Winqvist, R. (2006) · European Journal of Cancer · doi:10.1016/j.ejca.2006.05.030 · Cited by 42 · see citing works
Function of TopBP1 in Genome Stability
Sokka, M., Parkkinen, S., Pospiech, H., & Syväoja, J. E. (2010) · Subcellular Biochemistry · doi:10.1007/978-90-481-3471-7_7 · Cited by 38 · see citing works
The human ATR-mediated DNA damage checkpoint in a reconstituted system
Choi, J.-H., Sancar, A., & Lindsey-Boltz, L. A. (2009) · Methods · doi:10.1016/j.ymeth.2009.02.006 · Cited by 23 · see citing works
Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts
Bai, L., Michael, W. M., & Yan, S. (2014) · Cellular Signalling · doi:10.1016/j.cellsig.2014.01.006 · Cited by 19 · see citing works
WD40-repeat protein WDR18 collaborates with TopBP1 to facilitate DNA damage checkpoint signaling
Yan, S., & Willis, J. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2012.12.144 · Cited by 16 · see citing works
- Authors
- Garcia, V., Furuya, K., & Carr, A. M.
- Affiliations
- University of Sussex (GB)
- Corresponding author
- Carr, Antony M.
- Venue
- DNA Repair
- Publisher
- Elsevier BV
- Published
- 2005-11
- Volume
- 4
- Issue
- 11
- Pages
- 1227-1239
- ISSN
- 1568-7856 (ISSN-L)
- 1568-7864
- OpenAlex
- W2052410309
- Cited by
- 158
- References
- 102
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 9
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- Locations
- DNA repair (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Sussex Research Online (University of Sussex) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- TopBP1, BRCT domain, DNA replication initiation, DNA damage checkpoint, S-phase checkpoint, transcriptional regulation, DNA metabolism, yeast homolog
- MeSH terms
- Animals, Carrier Proteins, DNA-Binding Proteins, Humans, Nuclear Proteins, Saccharomyces cerevisiae, Schizosaccharomyces, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins, Structural Homology, Protein
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