Cut5 Is Required for the Binding of Atr and DNA Polymerase α to Genotoxin-damaged Chromatin
Open accessAbstract
DNA damage triggers the assembly of checkpoint signaling proteins on chromatin that activate the Chk1 signaling pathway and block S-phase progression. Here we show that genotoxin-induced Chk1 activation requires Cut5 (Mus101/TopBP1) in a process that is independent of the role of Cut5 in DNA replication. Analysis of the role of Cut5 in checkpoint activation revealed that it associated with chromatin following DNA damage in a process that required RPA. Additionally, Cut5 was required for the recruitment of Atr, DNA polymerase alpha, and Rad1 but not RPA to chromatin following DNA damage. Taken together, these results demonstrate that Cut5 plays an integral role in the recruitment and assembly of the Chk1 signaling cascade components following DNA damage.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
TopBP1 is required for recruiting ATR and the 9-1-1 complex onto genotoxin-damaged chromatin or stalled replication forks in Xenopus egg extracts and human cell lines.
“TopBP1 is required for the recruitment of ATR and the 9-1-1 complex onto genotoxin-damaged chromatin or stalled replication forks in Xenopus egg extracts and human cell lines [121–123].”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [121]
Cites
The DNA damage response: putting checkpoints in perspective
Zhou, B.-B. S., & Elledge, S. J. (2000) · Nature · doi:10.1038/35044005 · Cited by 3,213 · see citing works
p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21.
Toyoshima, H., & Hunter, T. (1994) · Cell · doi:10.1016/0092-8674(94)90573-8 · Cited by 2,208 · see citing works
Cell cycle checkpoint signaling through the ATM and ATR kinases
Abraham, R. T. (2001) · Genes & Development · doi:10.1101/gad.914401 · Cited by 1,966 · see citing works
(1999) · PubMed · Cited by 1,030 · see citing works
Chapter 30 Cell Cycle Extracts
Murray, A. W. (1991) · Methods in cell biology · doi:10.1016/s0091-679x(08)60298-8 · Cited by 942 · see citing works
Interfaces Between the Detection, Signaling, and Repair of DNA Damage
Rouse, J. W., & Jackson, S. P. (2002) · Science · doi:10.1126/science.1074740 · Cited by 688 · see citing works
Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatinPreprint
Zou, L., Cortez, D., & Elledge, S. J. (2002) · Genes & Development · doi:10.1101/gad.950302 · Cited by 498 · see citing works
Walter, J. C., & Newport, J. (2000) · Molecules and Cells · doi:10.1016/s1097-2765(00)80241-5 · Cited by 472 · see citing works
Kumagai, A., & Dunphy, W. G. (2000) · Molecular Cell · doi:10.1016/s1097-2765(05)00092-4 · Cited by 430 · see citing works
Involvement of nucleic acid synthesis in cell killing mechanisms of topoisomerase poisons.
D’Arpa, P., Beardmore, C., & Liu, L. F. (1990) · PubMed · Cited by 425 · see citing works
Two checkpoint complexes are independently recruited to sites of DNA damage in vivo
Melo, J. A., Cohen, J., & Toczyski, D. P. (2001) · Genes & Development · doi:10.1101/gad.903501 · Cited by 384 · see citing works
Caffeine inhibits the checkpoint kinase ATM
Blasina, A., Price, B. D., Turenne, G. A., & McGowan, C. H. (1999) · Current Biology · doi:10.1016/s0960-9822(99)80486-2 · Cited by 295 · see citing works
Zhou, B. S., Chaturvedi, P., Spring, K. J., Scott, S. P., Johanson, R. A., Mishra, R., Mattern, M. R., Winkler, J. D., & Khanna, K. K. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.14.10342 · Cited by 278 · see citing works
Araki, H., Leem, S., Phongdara, A., & Sugino, A. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.25.11791 · Cited by 273 · see citing works
Xenopus Cdc45‐dependent loading of DNA polymerase α onto chromatin under the control of S‐phase cdk
Mimura, S., & Takisawa, H. (1998) · EMBO Journal · doi:10.1093/emboj/17.19.5699 · Cited by 258 · see citing works
Heffernan, T. P., Simpson, D. A., Frank, A. R., Heinloth, A. N., Paules, R. S., Cordeiro-Stone, M., & Kaufmann, W. K. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.24.8552-8561.2002 · Cited by 251 · see citing works
Chk1‐deficient tumour cells are viable but exhibit multiple checkpoint and survival defects
Zachos, G., Rainey, M. D., & Gillespie, D. A. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg060 · Cited by 247 · see citing works
Saka, Y., & Yanagida, M. (1993) · Cell · doi:10.1016/0092-8674(93)90428-s · Cited by 233 · see citing works
BRCT Domain-containing Protein TopBP1 Functions in DNA Replication and Damage Response
Mäkiniemi, M., Hillukkala, T., Tuusa, J. T., Reini, K., Vaara, M., Huang, D., Pospiech, H., Majuri, I., Westerling, T., Mäkelä, T. P., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102245200 · Cited by 217 · see citing works
Activation of the DNA Replication Checkpoint Through RNA Synthesis by Primase
Michael, W. M., Ott, R., Fanning, E., & Newport, J. W. (2000) · Science · doi:10.1126/science.289.5487.2133 · Cited by 206 · see citing works
Lee, J., Kumagai, A., & Dunphy, W. G. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00045-5 · Cited by 199 · see citing works
Boyd, J. B., Golino, M. D., Nguyễn, D. T., et al. (1976) · Genetics · doi:10.1093/genetics/84.3.485 · Cited by 198 · see citing works
Hekmat-Nejad, M., You, Z., Yee, M., Newport, J. W., & Cimprich, K. A. (2000) · Current Biology · doi:10.1016/s0960-9822(00)00855-1 · Cited by 193 · see citing works
A DNA Damage-Regulated BRCT-Containing Protein, TopBP1, Is Required for Cell Survival
Yamane, K., Wu, X., & Chen, J. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.2.555-566.2002 · Cited by 181 · see citing works
A requirement for replication in activation of the ATR-dependent DNA damage checkpointPreprint
Lupardus, P. J., Byun, T. S., Yee, M., Hekmat-Nejad, M., & Cimprich, K. A. (2002) · Genes & Development · doi:10.1101/gad.1013502 · Cited by 163 · see citing works
A Role for Cdk2 Kinase in Negatively Regulating DNA Replication during S Phase of the Cell Cycle
Hua, X. H., Yan, H., & Newport, J. W. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.1.183 · Cited by 153 · see citing works
The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication
Hatten, R. A. V., Tutter, A., Holway, A. H., Khederian, A. M., Walter, J., & Michael, W. (2002) · Journal of Cell Biology · doi:10.1083/jcb.200207090 · Cited by 145 · see citing works
Xenopus Cut5 is essential for a CDK‐dependent process in the initiation of DNA replication
Hashimoto, Y., & Takisawa, H. (2003) · EMBO Journal · doi:10.1093/emboj/cdg238 · Cited by 140 · see citing works
Yamane, K., Kawabata, M., & TSURUO, T. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00794.x · Cited by 119 · see citing works
You, Z., Kong, L., & Newport, J. W. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204120200 · Cited by 114 · see citing works
DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts
Stokes, M. P., Hatten, R. A. V., Lindsay, H. D., & Michael, W. M. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200204127 · Cited by 82 · see citing works
Response ofXenopusCds1 in Cell-free Extracts to DNA Templates with Double-stranded Ends
Guo, Z., & Dunphy, W. G. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.5.1535 · Cited by 82 · see citing works
Carcinogen-induced S-phase arrest is Chk1 mediated and caffeine sensitive.
Guo, N., Faller, D. V., & Vaziri, C. (2002) · PubMed · Cited by 73 · see citing works
Yamamoto, R., Axton, M., Yamamoto, Y., Saunders, R. D. C., Glover, D. M., & Henderson, D. S. (2000) · Genetics · doi:10.1093/genetics/156.2.711 · Cited by 70 · see citing works
Shou, W.-G., Masuda, T., Takisawa, H., & Sugino, A. (2001) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.081088798 · Cited by 60 · see citing works
Stein, G. S., van Wijnen, A. J., Stein, J. L., Lian, J. B., McNeil, S. M., & Pockwinse, S. M. (1999) · Journal of Cellular Biochemistry · doi:10.1002/(sici)1097-4644(1999)75:32+ · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Dial 9–1–1 for DNA damage: the Rad9–Hus1–Rad1 (9–1–1) clamp complex
Parrilla-Castellar, E. R., Arlander, S. J. H., & Karnitz, L. (2004) · DNA Repair · doi:10.1016/j.dnarep.2004.03.032 · Cited by 342 · see citing works
Self-citationYan, S., Sorrell, M., & Berman, Z. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1666-4 · Cited by 247 · see citing works
Identification and functional analysis of TopBP1 and its homologs
Garcia, V., Furuya, K., & Carr, A. M. (2005) · DNA Repair · doi:10.1016/j.dnarep.2005.04.001 · Cited by 158 · see citing works
Yeast DNA replication protein Dpb11 activates the Mec1/ATR checkpoint kinase
Navadgi-Patil, V. M., & Burgers, P. M. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m807435200 · Cited by 121 · see citing works
Puddu, F., Granata, M., Di Nola, L., Balestrini, A., Piergiovanni, G., Lazzaro, F., Giannattasio, M., Plevani, P., & Muzi-Falconi, M. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.00330-08 · Cited by 115 · see citing works
TopBP1 and DNA polymerase-α directly recruit the 9-1-1 complex to stalled DNA replication forks
Yan, S., & Michael, W. M. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200810185 · Cited by 107 · see citing works
Navadgi-Patil, V. M., & Burgers, P. M. (2009) · DNA Repair · doi:10.1016/j.dnarep.2009.03.011 · Cited by 93 · see citing works
Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling: Figure 1.
Cortez, D. (2005) · Genes & Development · doi:10.1101/gad.1316905 · Cited by 84 · see citing works
Reconstitution of a human ATR-mediated checkpoint response to damaged DNA.
Choi, J.-H., Lindsey-Boltz, L. A., & Sancar, A. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706013104 · Cited by 64 · see citing works
Function of TopBP1 in Genome Stability
Sokka, M., Parkkinen, S., Pospiech, H., & Syväoja, J. E. (2010) · Subcellular Biochemistry · doi:10.1007/978-90-481-3471-7_7 · Cited by 38 · see citing works
- Authors
- Parrilla-Castellar, E. R., & Karnitz, L. M.
- Affiliations
- Mayo Clinic (US)
- Corresponding author
- Karnitz, Larry M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-11
- Volume
- 278
- Issue
- 46
- Pages
- 45507-45511
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1988571930
- Cited by
- 65
- References
- 36
- Citations by year
- 2020: 1 · 2019: 1 · 2017: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1f31ca099940-01)
- National Cancer Institute (F31 CA99940, T32CA075926)
- National Institute of General Medical Sciences (R25 GM5525)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- TopBP1, ATR kinase, DNA polymerase alpha, RAD1, DNA damage checkpoint, RPA, S-phase checkpoint
- MeSH terms
- Animals, Chromatin, DNA, DNA Damage, DNA Polymerase I, Models, Biological, Mutagens, Protein Kinases, Xenopus, Signal Transduction, S Phase, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Drosophila Proteins, Xenopus Proteins, Ataxia Telangiectasia Mutated Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review