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Cited by 50Cellular Mechanics and InteractionsProtein Kinase Regulation and GTPase SignalingMicrotubule and mitosis dynamics

Branching out in different directions: Emerging cellular functions for the Arp2/3 complex and WASP-family actin nucleation factors

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Campellone, Kenneth G., Lebek, Nadine M., King, Virginia L.·2023·European Journal of Cell Biology
DOI 10.1016/j.ejcb.2023.151301PMC PMC10330494
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Abstract

The actin cytoskeleton impacts practically every function of a eukaryotic cell. Historically, the best-characterized cytoskeletal activities are in cell morphogenesis, motility, and division. The structural and dynamic properties of the actin cytoskeleton are also crucial for establishing, maintaining, and changing the organization of membrane-bound organelles and other intracellular structures. Such activities are important in nearly all animal cells and tissues, although distinct anatomical regions and physiological systems rely on different regulatory factors. Recent work indicates that the Arp2/3 complex, a broadly expressed actin nucleator, drives actin assembly during several intracellular stress response pathways. These newly described Arp2/3-mediated cytoskeletal rearrangements are coordinated by members of the Wiskott-Aldrich Syndrome Protein (WASP) family of actin nucleation-promoting factors. Thus, the Arp2/3 complex and WASP-family proteins are emerging as crucial players in cytoplasmic and nuclear activities including autophagy, apoptosis, chromatin dynamics, and DNA repair. Characterizations of the functions of the actin assembly machinery in such stress response mechanisms are advancing our understanding of both normal and pathogenic processes, and hold great promise for providing insights into organismal development and interventions for disease.

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  • WAVE members are key activators of ARP2/3 complex-mediated actin polymerization.

    “Wiskott‒Aldrich syndrome protein-family verprolin-homologous protein (WAVE) members are key activators of ARP2/3 complex-mediated actin polymerization.”

    From The WAVE complex in developmental and adulthood brain disorders (2025) · cites this paper as [7]

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  1. Abella JVG, Galloni C, Pernier J, Barry DJ, Kjaer S, Carlier M-F, Way M, 2016. Isoform diversity in the Arp2/3 complex determines actin filament dynamics. Nat. Cell Biol. 18, 76– 88. 10.1038/ncb3286. 26655834 · DOI
  2. Ahn JS, Jang I-S, Kim D-I, Cho KA, Park YH, Kim K, Kwak CS, Chul Park S, 2003. Aging-associated increase of gelsolin for apoptosis resistance. Biochem. Biophys. Res. Commun. 312, 1335– 1341. 10.1016/j.bbrc.2003.11.061. 14652020 · DOI
  3. Aiuti A, Biasco L, Scaramuzza S, Ferrua F, Cicalese MP, Baricordi C, Dionisio F, Calabria A, Giannelli S, Castiello MC, Bosticardo M, Evangelio C, Assanelli A, Casiraghi M, Di Nunzio S, Callegaro L, Benati C, Rizzardi P, Pellin D, Di Serio C, Schmidt M, Von Kalle C, Gardner J, Mehta N, Neduva V, Dow DJ, Galy A, Miniero R, Finocchi A, Metin A, Banerjee PP, Orange JS, Galimberti S, Valsecchi MG, Biffi A, Montini E, Villa A, Ciceri F, Roncarolo MG, Naldini L, 2013. Lentiviral hematopoietic stem cell gene therapy in patients with Wiskott-Aldrich syndrome. Science 341, 1233151. 10.1126/science.1233151. 23845947 PMC4375961 · DOI · Paper page
  4. Akamatsu M, Vasan R, Serwas D, Ferrin MA, Rangamani P, Drubin DG, 2020. Principles of self-organization and load adaptation by the actin cytoskeleton during clathrin-mediated endocytosis. eLife 9, e49840. 10.7554/eLife.49840. 31951196 PMC7041948 · DOI · Paper page
  5. Alekhina O, Burstein E, Billadeau DD, 2017. Cellular functions of WASP family proteins at a glance. J. Cell Sci. 130, 2235– 2241. 10.1242/jcs.199570. 28646090 PMC5536917 · DOI
  6. Alexandrova AY, Chikina AS, Svitkina TM, 2020. Actin cytoskeleton in mesenchymal-to-amoeboid transition of cancer cells. Int. Rev. Cell Mol. Biol. 356, 197– 256. 10.1016/bs.ircmb.2020.06.002. 33066874 PMC9038018 · DOI
  7. Algeciras-Schimnich A, Shen L, Barnhart BC, Murmann AE, Burkhardt JK, Peter ME, 2002. Molecular ordering of the initial signaling events of CD95. Mol. Cell. Biol. 22, 207– 220. 10.1128/MCB.22.1.207-220.2002. 11739735 PMC134211 · DOI
  8. Aloisio FM, Barber DL, 2022. Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification. Stem Cell Rep. 17, 1318– 1333. 10.1016/j.stemcr.2022.05.002. PMC9214060 35658973 · DOI
  9. Andrysik Z, Galbraith MD, Guarnieri AL, Zaccara S, Sullivan KD, Pandey A, MacBeth M, Inga A, Espinosa JM, 2017. Identification of a core TP53 transcriptional program with highly distributed tumor suppressive activity. Genome Res 27, 1645– 1657. 10.1101/gr.220533.117. 28904012 PMC5630028 · DOI
  10. Aplin A, Jasionowski T, Tuttle DL, Lenk SE, Dunn WA, 1992. Cytoskeletal elements are required for the formation and maturation of autophagic vacuoles. J. Cell. Physiol. 152, 458– 466. 10.1002/jcp.1041520304. 1506410 · DOI
  11. Ardern H, Sandilands E, Machesky LM, Timpson P, Frame MC, Brunton VG, 2006. Src-dependent phosphorylation of Scar1 promotes its association with the Arp2/3 complex. Cell Motil. Cytoskelet. 63, 6– 13. 10.1002/cm.20101. 16317717 · DOI
  12. Azoury J, Lee KW, Georget V, Rassinier P, Leader B, Verlhac M-H, 2008. Spindle positioning in mouse oocytes relies on a dynamic meshwork of actin filaments. Curr. Biol. 18, 1514– 1519. 10.1016/j.cub.2008.08.044. 18848445 · DOI
  13. Ballabio A, Bonifacino JS, 2020. Lysosomes as dynamic regulators of cell and organismal homeostasis. Nat. Rev. Mol. Cell Biol. 21, 101– 118. 10.1038/s41580-019-0185-4. 31768005 · DOI · Paper page
  14. Banani SF, Lee HO, Hyman AA, Rosen MK, 2017. Biomolecular condensates: organizers of cellular biochemistry. Nat. Rev. Mol. Cell Biol. 18, 285– 298. 10.1038/nrm.2017.7. 28225081 PMC7434221 · DOI
  15. Bathe FS, Rommelaere H, Machesky LM, 2007. Phenotypes of myopathy-related actin mutants in differentiated C2C12 myotubes. BMC Cell Biol. 8, 2. 10.1186/1471-2121-8-2. 17227580 PMC1779783 · DOI
  16. Bear JE, Rawls JF, Saxe CL, 1998. SCAR, a WASP-related Protein, Isolated as a Suppressor of Receptor Defects in Late Dictyostelium Development. J. Cell Biol. 142, 1325– 1335. 10.1083/jcb.142.5.1325. 9732292 PMC2149354 · DOI
  17. Belin BJ, Goins LM, Mullins RD, 2014. Comparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture 4, 189– 202. 10.1080/19490992.2014.1047714. 26317264 PMC4914014 · DOI
  18. Belin BJ, Lee T, Mullins RD, 2015. DNA damage induces nuclear actin filament assembly by Formin −2 and Spire-½ that promotes efficient DNA repair. eLife 4, e07735. 10.7554/eLife.07735. 26287480 PMC4577826 · DOI
  19. Benesch S, Lommel S, Steffen A, Stradal TEB, Scaplehorn N, Way M, Wehland J, Rottner K, 2002. Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2. J. Biol. Chem. 277, 37771– 37776. 10.1074/jbc.M204145200. 12147689 · DOI
  20. Benesch S, Polo S, Lai FPL, Anderson KI, Stradal TEB, Wehland J, Rottner K, 2005. N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits. J. Cell Sci. 118, 3103– 3115. 10.1242/JCS.02444. 15985465 · DOI
  21. Ben-Omran T, Fahiminiya S, Sorfazlian N, Almuriekhi M, Nawaz Z, Nadaf J, Khadija KA, Zaineddin S, Kamel H, Majewski J, Tropepe V, 2015. Nonsense mutation in the WDR73 gene is associated with Galloway-Mowat syndrome. J. Med. Genet 52, 381– 390. 10.1136/jmedgenet-2014-102707. 25873735 · DOI
  22. Bhola PD, Mattheyses AL, Simon SM, 2009. Spatial and temporal dynamics of mitochondrial membrane permeability waves during apoptosis. Biophys. J. 97, 2222– 2231. 10.1016/j.bpj.2009.07.056. 19843454 PMC2764100 · DOI
  23. Biber G, Ben-Shmuel A, Sabag B, Barda-Saad M, 2020. Actin regulators in cancer progression and metastases: From structure and function to cytoskeletal dynamics. Int. Rev. Cell Mol. Biol. 356, 131– 196. 10.1016/bs.ircmb.2020.05.006. 33066873 · DOI
  24. Blom M, Reis K, Nehru V, Blom H, Gad AKB, Aspensträm P, 2015. RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane. Exp. Cell Res. 333, 208– 219. 10.1016/j.yexcr.2015.02.023. 25746724 · DOI
  25. Bock FJ, Tait SWG, 2020. Mitochondria as multifaceted regulators of cell death. Nat. Rev. Mol. Cell Biol. 21, 85– 100. 10.1038/s41580-019-0173-8. 31636403 · DOI
  26. Boice A, Bouchier-Hayes L, 2020. Targeting apoptotic caspases in cancer. Biochim. Biophys. Acta Mol. Cell Res. 1867, 118688 10.1016/j.bbamcr.2020.118688. 32087180 PMC7155770 · DOI
  27. Bouma G, Burns SO, Thrasher AJ, 2009. Wiskott–Aldrich Syndrome: Immunodeficiency resulting from defective cell migration and impaired immunostimulatory activation. Immunobiology 214, 778– 790. 10.1016/j.imbio.2009.06.009. 19628299 PMC2738782 · DOI
  28. Braun DA, Rao J, Mollet G, Schapiro D, Daugeron M-C, Tan W, Gribouval O, Boyer O, Revy P, Jobst-Schwan T, Schmidt JM, Lawson JA, Schanze D, Ashraf S, Boddaert N, Collinet B, Martin G, Liger D, Lovric S, Furlano M, Guerrera IC, Sanchez-Ferras O, Menten B, Vergult S, De Rocker N, Airik M, Hermle T, Shril S, Widmeier E, Gee HY, Choi W-I, Sadowski CE, Pabst WL, Warejko J, Daga A, LeBerre TB, Matejas V, Behnam B, Beeson B, Begtrup A, Bruce M, Ch’ng G-S, Lin S-P, Chang J-H, Chen C-H, Cho MT, Gipson PE, Hsu C-H, Kari JA, Ke Y-Y, Kiraly-Borri C, Lai W, Lemyre E, Littlejohn RO, Masri A, Moghtaderi M, Nakamura K, Praet M, Prasad C, Prytula A, Roeder E, Rump P, Schnur RE, Shiihara T, Sinha M, Soliman NA, Soulami K, Sweetser DA, Tsai W-H, Tsai J-D, Vester U, Viskochil DH, Vatanavicharn N, Waxler JL, Wolf MTF, Wong S-N, Poduri A, Truglio G, Mane S, Lifton RP, Bouchard M, Kannu P, Chitayat D, Magen D, Calleweart B, van Tilbeurgh H, Zenker M, Antignac C, Hildebrandt F, 2017. Mutations in the evolutionarily highly conserved KEOPS complex genes cause nephrotic syndrome with microcephaly. Nat. Genet 49, 1529– 1538. 10.1038/ng.3933. 28805828 PMC5819591 · DOI
  29. Braun DA, Shril S, Sinha A, Schneider R, Tan W, Ashraf S, Hermle T, Jobst-Schwan T, Widmeier E, Majmundar AJ, Daga A, Warejko JK, Nakayama M, Schapiro D, Chen J, Airik M, Rao J, Schmidt JM, Hoogstraten CA, Hugo H, Meena J, Lek M, Laricchia KM, Bagga A, Hildebrandt F, 2018. Mutations in WDR4 as a new cause of Galloway-Mowat syndrome. Am. J. Med. Genet. A 176, 2460– 2465. 10.1002/ajmg.a.40489. 30079490 PMC6289609 · DOI
  30. Breitsprecher D, Goode BL, 2013. Formins at a glance. J. Cell Sci. 126, 1– 7. 10.1242/jcs.107250. 23516326 PMC3603506 · DOI
  31. Brigida I, Zoccolillo M, Cicalese MP, Pfajfer L, Barzaghi F, Scala S, Oleaga-Quintas C, Álvarez-Álvarez JA, Sereni L, Giannelli S, Sartirana C, Dionisio F, Pavesi L, Benavides-Nieto M, Basso-Ricci L, Capasso P, Mazzi B, Rosain J, Marcus N, Lee YN, Somech R, Degano M, Raiola G, Caorsi R, Picco P, Moncada Velez M, Khourieh J, Arias AA, Bousfiha A, Issekutz T, Issekutz A, Boisson B, Dobbs K, Villa A, Lombardo A, Neven B, Moshous D, Casanova J-L, Franco JL, Notarangelo LD, Scielzo C, Volpi S, Dupré L, Bustamante J, Gattorno M, Aiuti A, 2018. T-cell defects in patients with ARPC1B germline mutations account for combined immunodeficiency. Blood 132, 2362– 2374. 10.1182/blood-2018-07-863431. 30254128 PMC6265646 · DOI
  32. Bu W, Lim KB, Yu YH, Chou AM, Sudhaharan T, Ahmed S, 2010. Cdc42 interaction with N-WASP and Toca-1 regulates membrane tubulation, vesicle formation and vesicle motility: implications for endocytosis. PloS One 5, e12153. 10.1371/journal.pone.0012153. 20730103 PMC2921345 · DOI
  33. Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, Lippincott-Schwartz J, 2014. A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. J. Cell Biol. 205, 83– 96. 10.1083/jcb.201311104. 24711500 PMC3987145 · DOI
  34. Burridge K, Guilluy C, 2016. Focal adhesions, stress fibers and mechanical tension. Exp. Cell Res. 343, 14– 20. 10.1016/j.yexcr.2015.10.029. 26519907 PMC4891215 · DOI · Paper page
  35. Campellone KG, Welch MD, 2010. A nucleator arms race: Cellular control of actin assembly. Nat. Rev. Mol. Cell Biol. 11, 237– 251. 10.1038/nrm2867. 20237478 PMC2929822 · DOI
  36. Campellone KG, Cheng H-C, Robbins D, Siripala AD, McGhie EJ, Hayward RD, Welch MD, Rosen MK, Koronakis V, Leong JM, 2008a. Repetitive N-WASP-binding elements of the enterohemorrhagic Escherichia coli effector EspF(U) synergistically activate actin assembly. PLoS Pathog. 4, e1000191 10.1371/journal.ppat.1000191. 18974829 PMC2567903 · DOI
  37. Campellone KG, Webb NJ, Znameroski EA, Welch MD, 2008b. WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport. Cell 134, 148– 161. 10.1016/j.cell.2008.05.032. 18614018 PMC2556884 · DOI
  38. Cao L, Yonis A, Vaghela M, Barriga EH, Chugh P, Smith MB, Maufront J, Lavoie G, Méant A, Ferber E, Bovellan M, Alberts A, Bertin A, Mayor R, Paluch EK, Roux PP, Jégou A, Romet-Lemonne G, Charras G, 2020. SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization. Nat. Cell Biol. 22, 803– 814. 10.1038/s41556-020-0531-y. 32572169 · DOI
  39. Caridi CP, D’Agostino C, Ryu T, Zapotoczny G, Delabaere L, Li X, Khodaverdian VY, Amaral N, Lin E, Rau AR, Chiolo I, 2018. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature 559, 54– 60. 10.1038/s41586-018-0242-8. 29925946 PMC6051730 · DOI · Paper page
  40. Caridi CP, Plessner M, Grosse R, Chiolo I, 2019. Nuclear actin filaments in DNA repair dynamics. Nat. Cell Biol. 21, 1068– 1077. 10.1038/s41556-019-0379-1. 31481797 PMC6736642 · DOI · Paper page
  41. Carlsson SR, Simonsen A, 2015. Membrane dynamics in autophagosome biogenesis. J. Cell Sci. 128, 198– 205. 10.1242/jcs.141036. 25568151 · DOI
  42. Carneiro BA, El-Deiry WS, 2020. Targeting apoptosis in cancer therapy. Nat. Rev. Clin. Oncol. 17, 395– 417. 10.1038/s41571-020-0341-y. 32203277 PMC8211386 · DOI
  43. Case LB, Waterman CM, 2015. Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch. Nat. Cell Biol. 17, 955– 963. 10.1038/ncb3191. 26121555 PMC6300998 · DOI · Paper page
  44. Chakrabarti R, Lee M, Higgs HN, 2021. Multiple roles for actin in secretory and endocytic pathways. Curr. Biol. 31, R603– R618. 10.1016/J.CUB.2021.03.038. 34033793 PMC9759210 · DOI
  45. Charras GT, Hu C-K, Coughlin M, Mitchison TJ, 2006. Reassembly of contractile actin cortex in cell blebs. J. Cell Biol. 175, 477– 490. 10.1083/jcb.200602085. 17088428 PMC2064524 · DOI
  46. Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK, 2014. The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton. Cell 156, 195. 10.1016/J.CELL.2013.11.048. 24439376 PMC4059610 · DOI · Paper page
  47. Chen B, Chou H-T, Brautigam CA, Xing W, Yang S, Henry L, Doolittle LK, Walz T, Rosen MK, 2017. Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites. eLife 6, e29795. 10.7554/eLife.29795. 28949297 PMC5614565 · DOI · Paper page
  48. Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK, 2010. Structure and control of the actin regulatory WAVE complex, 4687323 468 Nature 2010, 533– 538. 10.1038/nature09623. PMC3085272 21107423 · DOI · Paper page
  49. Cheng A, Arumugam TV, Liu D, Khatri RG, Mustafa K, Kwak S, Ling H-P, Gonzales C, Xin O, Jo D-G, Guo Z, Mark RJ, Mattson MP, 2007. Pancortin-2 interacts with WAVE1 and Bcl-xL in a mitochondria-associated protein complex that mediates ischemic neuronal death. J. Neurosci. 27, 1519– 1528. 10.1523/JNEUROSCI.5154-06.2007. 17301160 PMC6673736 · DOI · Paper page
  50. Cheng H-C, Skehan BM, Campellone KG, Leong JM, Rosen MK, 2008. Structural Mechanism of WASP Activation by the Enterohaemorrhagic E. coli Effector EspFU. Nature 454, 1009– 1013. 10.1038/nature07160. 18650809 PMC2719906 · DOI
  51. Chhabra D, Nosworthy NJ, dos Remedios CG, 2005. The N-terminal fragment of gelsolin inhibits the interaction of DNase I with isolated actin, but not with the cofilin-actin complex. Proteomics 5, 3131– 3136. 10.1002/pmic.200401127. 16021605 · DOI
  52. Chorev DS, Moscovitz O, Geiger B, Sharon M, 2014. Regulation of focal adhesion formation by a vinculin-Arp2/3 hybrid complex. Nat. Commun. 5, 3758. 10.1038/ncomms4758. 24781749 · DOI
  53. Chua BT, Volbracht C, Tan KO, Li R, Yu VC, Li P, 2003. Mitochondrial translocation of cofilin is an early step in apoptosis induction. Nat. Cell Biol. 5, 1083– 1089. 10.1038/ncb1070. 14634665 · DOI
  54. Cioca DP, Kitano K, 2002. Induction of apoptosis and CD10/neutral endopeptidase expression by jaspamide in HL-60 line cells. Cell. Mol. Life Sci. 59, 1377– 1387. 10.1007/s00018-002-8515-6. 12363040 PMC11337437 · DOI
  55. Clarkson E, Costa CF, Machesky LM, 2004. Congenital myopathies: diseases of the actin cytoskeleton. J. Pathol. 204, 407– 417. 10.1002/path.1648. 15495263 · DOI
  56. Colin E, Huynh Cong E, Mollet G, Guichet A, Gribouval O, Arrondel C, Boyer O, Daniel L, Gubler M-C, Ekinci Z, Tsimaratos M, Chabrol B, Boddaert N, Verloes A, Chevrollier A, Gueguen N, Desquiret-Dumas V, Ferré M, Procaccio V, Richard L, Funalot B, Moncla A, Bonneau D, Antignac C, 2014. Loss-of-Function Mutations in WDR73 Are Responsible for Microcephaly and Steroid-Resistant Nephrotic Syndrome: Galloway-Mowat Syndrome. Am. J. Hum. Genet 95, 637– 648. 10.1016/j.ajhg.2014.10.011. 25466283 PMC4259970 · DOI
  57. Cory GOC, Garg R, Cramer R, Ridley AJ, 2002. Phosphorylation of tyrosine 291 enhances the ability of WASp to stimulate actin polymerization and filopodium formation. Wiskott-Aldrich Syndrome protein. J. Biol. Chem. 277, 45115– 45121. 10.1074/jbc.M203346200. 12235133 · DOI
  58. Costa CF, Rommelaere H, Waterschoot D, Sethi KK, Nowak KJ, Laing NG, Ampe C, Machesky LM, 2004. Myopathy mutations in alpha-skeletal-muscle actin cause a range of molecular defects. J. Cell Sci. 117, 3367– 3377. 10.1242/jcs.01172. 15226407 · DOI
  59. Courtemanche N, 2018. Mechanisms of formin-mediated actin assembly and dynamics. Biophys. Rev. 10, 1553– 1569. 10.1007/s12551-018-0468-6. 30392063 PMC6297096 · DOI
  60. Courtland JL, Bradshaw TW, Waitt G, Soderblom EJ, Ho T, Rajab A, Vancini R, Kim IH, Soderling SH, 2021. Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and cognitive-movement impairments in mice and humans. eLife 10. 10.7554/ELIFE.61590. PMC7984842 33749590 · DOI
  61. Coutts AS, La Thangue NB, 2015. Actin nucleation by WH2 domains at the autophagosome. Nat. Commun. 6. 10.1038/ncomms8888. PMC4532831 26223951 · DOI
  62. Coutts AS, Boulahbel H, Graham A, La Thangue NB, 2007. Mdm2 targets the p53 transcription cofactor JMY for degradation. EMBO Rep. 8, 84– 90. 10.1038/sj.embor.7400855. 17170761 PMC1796743 · DOI
  63. Chou SZ, Chatterjee M, Pollard TD, 2022, Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structure of the branch junction. Proc. Natl. Acad. Sci. U. S. A. 119, e2206722119. 10.1073/pnas.2206722119. 36442092 PMC9894260 · DOI
  64. Coutts AS, Weston L, La Thangue NB, 2009, A transcription co-factor integrates cell adhesion and motility with the p53 response. Proc. Natl. Acad. Sci. U. S. A. 106, 19872– 19877. 10.1073/pnas.0906785106. 19897726 PMC2785259 · DOI
  65. Czuchra A, Wu X, Meyer H, van Hengel J, Schroeder T, Geffers R, Rottner K, Brakebusch C, 2005. Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells. Mol. Biol. Cell 16, 4473– 4484. 10.1091/mbc.E05-01-0061. 16014609 PMC1237057 · DOI
  66. Dai A, Yu L, Wang HW, 2019. WHAMM initiates autolysosome tubulation by promoting actin polymerization on autolysosomes. Nat. Commun. 10. 10.1038/S41467-019-11694-9. PMC6697732 31420534 · DOI
  67. Danial NN, Gramm CF, Scorrano L, Zhang C-Y, Krauss S, Ranger AM, Datta SR, Greenberg ME, Licklider LJ, Lowell BB, Gygi SP, Korsmeyer SJ, 2003. BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424, 952– 956. 10.1038/nature01825. 12931191 · DOI · Paper page
  68. Davidson PM, Cadot B, 2021. Actin on and around the Nucleus. Trends Cell Biol. 31, 211– 223. 10.1016/j.tcb.2020.11.009. 33376040 · DOI
  69. Demonacos C, Krstic-Demonacos M, La Thangue NB, 2001. A TPR motif cofactor contributes to p300 activity in the p53 response. Mol. Cell 8, 71– 84. 10.1016/s1097-2765(01)00277-5. 11511361 · DOI
  70. Derivery E, Sousa C, Gautier JJ, Lombard B, Loew D, Gautreau A, 2009. The Arp2/3 Activator WASH Controls the Fission of Endosomes through a Large Multiprotein Complex. Dev. Cell 17, 712– 723. 10.1016/J.DEVCEL.2009.09.010. 19922875 · DOI
  71. Derry JM, Ochs HD, Francke U, 1994. Isolation of a Novel Gene Mutated in Wiskott-Aldrich Syndrome. Cell 76, 635– 644. 8069912
  72. Desouza M, Gunning PW, Stehn JR, 2012. The actin cytoskeleton as a sensor and mediator of apoptosis. Bioarchitecture 2, 75– 87. 10.4161/bioa.20975. 22880146 PMC3414384 · DOI
  73. Dickens LS, Powley IR, Hughes MA, MacFarlane M, 2012. The “complexities” of life and death: death receptor signalling platforms. Exp. Cell Res. 318, 1269– 1277. 10.1016/j.yexcr.2012.04.005. 22542855 · DOI · Paper page
  74. Diepstraten ST, Anderson MA, Czabotar PE, Lessene G, Strasser A, Kelly GL, 2022. The manipulation of apoptosis for cancer therapy using BH3-mimetic drugs. Nat. Rev. Cancer 22, 45– 64. 10.1038/s41568-021-00407-4. 34663943 · DOI
  75. Dimchev V, Lahmann I, Koestler SA, Kage F, Dimchev G, Steffen A, Stradal TEB, Vauti F, Arnold H-H, Rottner K, 2021. Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation. Front. Cell Dev. Biol. 9, 634708 10.3389/fcell.2021.634708. 33598464 PMC7882613 · DOI
  76. Ding B, Narvaez-Ortiz HY, Singh Y, Hocky GM, Chowdhury S, Nolen BJ, 2022, Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy. Proc. Natl. Acad. Sci. U. S. A. 119, e2202723119. 10.1073/pnas.2202723119. 35622886 PMC9295785 · DOI
  77. Dobramysl U, Jarsch IK, Inoue Y, Shimo H, Richier B, Gadsby JR, Mason J, Szałapak A, Ioannou PS, Correia GP, Walrant A, Butler R, Hannezo E, Simons BD, Gallop JL, 2021. Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. J. Cell Biol. 220, e202003052 10.1083/jcb.202003052. 33740033 PMC7980258 · DOI
  78. Domazetovska A, Ilkovski B, Kumar V, Valova VA, Vandebrouck A, Hutchinson DO, Robinson PJ, Cooper ST, Sparrow JC, Peckham M, North KN, 2007. Intranuclear rod myopathy: molecular pathogenesis and mechanisms of weakness. Ann. Neurol. 62, 597– 608. 10.1002/ana.21200. 17705262 · DOI
  79. Domingo-Gallego A, Furlano M, Pybus M, Barraca D, Martínez AB, Mora Muñoz E, Torra R, Ars E, 2019. Novel homozygous OSGEP gene pathogenic variants in two unrelated patients with Galloway-Mowat syndrome: case report and review of the literature. BMC Nephrol. 20, 126. 10.1186/s12882-019-1317-y. 30975089 PMC6458604 · DOI
  80. Dominguez R, 2016. The WH2 domain and actin nucleation: necessary but insufficient. Trends Biochem. Sci. 41, 478– 490. 10.1016/j.tibs.2016.03.004. 27068179 PMC4884163 · DOI
  81. Dorstyn L, Akey CW, Kumar S, 2018. New insights into apoptosome structure and function. Cell Death Differ. 25, 1194– 1208. 10.1038/s41418-017-0025-z. 29765111 PMC6030056 · DOI
  82. Dubielecka PM, Ladwein KI, Xiong X, Migeotte I, Chorzalska A, Anderson KV, Sawicki JA, Rottner K, Stradal TE, Kotula L, 2011. Essential role for Abi1 in embryonic survival and WAVE2 complex integrity. Proc. Natl. Acad. Sci. U. S. A. 108, 7022– 7027. 10.1073/pnas.1016811108. 21482783 PMC3084068 · DOI · Paper page
  83. Duleh SN, Welch MD, 2010. WASH and the Arp2/3 complex regulate endosome shape and trafficking. Cytoskelet 67, 193. 10.1002/CM.20437. PMC2887680 20175130 · DOI
  84. Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW, 2002. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature 418, 790– 793. 10.1038/nature00859. 12181570 · DOI · Paper page
  85. Fais S, De Milito A, Lozupone F, 2005. The role of FAS to ezrin association in FAS-mediated apoptosis. Apoptosis 10, 941– 947. 10.1007/s10495-005-0478-2. 16151629 · DOI
  86. Faix J, Rottner K, 2022. Ena/VASP proteins in cell edge protrusion, migration and adhesion. J. Cell Sci. 135, jcs259226 10.1242/jcs.259226. 35285496 · DOI
  87. Farina F, Gaillard J, Guérin C, Couté Y, Sillibourne J, Blanchoin L, Théry M, 2016. The centrosome is an actin-organizing centre. Nat. Cell Biol. 18, 65– 75. 10.1038/ncb3285. 26655833 PMC4880044 · DOI
  88. Farina F, Ramkumar N, Brown L, Samandar Eweis D, Anstatt J, Waring T, Bithell J, Scita G, Thery M, Blanchoin L, Zech T, Baum B, 2019. Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis. EMBO J. 38, e99843 10.15252/embj.201899843. 31015335 PMC6545563 · DOI
  89. Fäßler F, Javoor MG, Datler J, Döring H, Hofer FW, Dimchev G, Hodirnau V-V, Faix J, Rottner K, Schur FKM, 2023. ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning. Sci. Adv. 9, eadd6495. 10.1126/sciadv.add6495. 36662867 PMC9858492 · DOI
  90. Fiore APZP, Spencer VA, Mori H, Carvalho HF, Bissell MJ, Bruni-Cardoso A, 2017. Laminin-111 and the Level of Nuclear Actin Regulate Epithelial Quiescence via Exportin-6. Cell Rep. 19, 2102– 2115. 10.1016/j.celrep.2017.05.050. 28591581 PMC6663100 · DOI
  91. Fischer RS, Lam PY, Huttenlocher A, Waterman CM, 2019. Filopodia and focal adhesions: An integrated system driving branching morphogenesis in neuronal pathfinding and angiogenesis. Dev. Biol. 451, 86. 10.1016/J.YDBIO.2018.08.015. 30193787 PMC7082808 · DOI
  92. Fokin AI, Gautreau AM, 2021. Assembly and Activity of the WASH Molecular Machine: Distinctive Features at the Crossroads of the Actin and Microtubule Cytoskeletons. Front. Cell Dev. Biol. 9, 658865 10.3389/fcell.2021.658865. 33869225 PMC8047104 · DOI
  93. Fokin AI, David V, Oguievetskaia K, Derivery E, Stone CE, Cao L, Rocques N, Molinie N, Henriot V, Aumont-Nicaise M, Hinckelmann M-V, Saudou F, Le Clainche C, Carter AP, Romet-Lemonne G, Gautreau AM, 2021. The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex. Sci. Adv. 7, eabd5956 10.1126/sciadv.abd5956. 33523880 PMC7806238 · DOI
  94. Fuentes-Prior P, Salvesen GS, 2004. The protein structures that shape caspase activity, specificity, activation and inhibition. Biochem. J. 384, 201– 232. 10.1042/BJ20041142. 15450003 PMC1134104 · DOI
  95. Fung TS, Ji W-K, Higgs HN, Chakrabarti R, 2019. Two distinct actin filament populations have effects on mitochondria, with differences in stimuli and assembly factors. J. Cell Sci. 132, jcs234435. 10.1242/jcs.234435. 31413070 PMC6765187 · DOI
  96. Fung TS, Chakrabarti R, Kollasser J, Rottner K, Stradal TEB, Kage F, Higgs HN, 2022. Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria. e8 Curr. Biol. CB 32, 1577– 1592. 10.1016/j.cub.2022.02.058. PMC9078333 35290799 · DOI
  97. Galloni C, Carra D, Abella JVG, Kjær S, Singaravelu P, Barry DJ, Kogata N, Guérin C, Blanchoin L, Way M, 2021. MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes. J. Cell Biol. 220, e202102043 10.1083/jcb.202102043. 34106209 PMC8193582 · DOI
  98. Gallop JL, 2020. Filopodia and their links with membrane traffic and cell adhesion. Semin. Cell Dev. Biol. 102, 81– 89. 10.1016/J.SEMCDB.2019.11.017. 31843255 · DOI
  99. Gardel ML, Schneider IC, Aratyn-Schaus Y, Waterman CM, 2010. Mechanical Integration of Actin and Adhesion Dynamics in Cell Migration. Annu. Rev. Cell Dev. Biol. 26, 315– 333. 10.1146/annurev.cellbio.011209.122036. 19575647 PMC4437624 · DOI
  100. Gatica D, Lahiri V, Klionsky DJ, 2018. Cargo recognition and degradation by selective autophagy. Nat. Cell Biol. 20, 233– 242. 10.1038/s41556-018-0037-z. 29476151 PMC6028034 · DOI · Paper page
  101. Gautreau A, Ho HH, Li J, Steen H, Gygi SP, Kirschner MW, 2004, Purification and architecture of the ubiquitous Wave complex. Proc. Natl. Acad. Sci. U. S. A. 101, 4379– 4383. 10.1073/pnas.0400628101. 15070726 PMC384755 · DOI
  102. Gautreau AM, Fregoso FE, Simanov G, Dominguez R, 2022. Nucleation, stabilization, and disassembly of branched actin networks. Trends Cell Biol. 32, 421– 432. 10.1016/j.tcb.2021.10.006. 34836783 PMC9018471 · DOI
  103. Genescá M, Sola A, Hotter G, 2006. Actin cytoskeleton derangement induces apoptosis in renal ischemia/reperfusion. Apoptosis 11, 563– 571. 10.1007/s10495-006-4937-1. 16528472 · DOI
  104. Gligorijevic B, Wyckoff J, Yamaguchi H, Wang Y, Roussos ET, Condeelis J, 2012. N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors. J. Cell Sci. 125, 724– 734. 10.1242/jcs.092726. 22389406 PMC3367832 · DOI
  105. Gomez TS, Billadeau DD, 2009. A FAM21-Containing WASH Complex Regulates Retromer-Dependent Sorting. Dev. Cell 17, 699. 10.1016/J.DEVCEL.2009.09.009. 19922874 PMC2803077 · DOI
  106. Gomez TS, Gorman JA, de Narvajas AAM, Koenig AO, Billadeau DD, 2012. Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. Mol. Biol. Cell 23, 3215. 10.1091/MBC.E12-02-0101. 22718907 PMC3418315 · DOI
  107. Gordon DJ, Resio B, Pellman D, 2012. Causes and consequences of aneuploidy in cancer. Nat. Rev. Genet. 13, 189– 203. 10.1038/nrg3123. 22269907 · DOI
  108. Gourlay CW, Ayscough KR, 2005. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. Mol. Cell Biol. 6, 583– 589. 10.1038/nrm1682. 16072039 · DOI
  109. Gruenbaum-Cohen Y, Harel I, Umansky K-B, Tzahor E, Snapper SB, Shilo B-Z, Schejter ED, 2012, The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc. Natl. Acad. Sci. U. S. A. 109, 11211– 11216. 10.1073/pnas.1116065109. 22736793 PMC3396508 · DOI
  110. Haarer B, Aggeli D, Viggiano S, Burke DJ, Amberg DC, 2011. Novel Interactions between Actin and the Proteasome Revealed by Complex Haploinsufficiency. PLoS Genet 7, e1002288. 10.1371/JOURNAL.PGEN.1002288. 21966278 PMC3178594 · DOI
  111. Haarer EL, Theodore CJ, Guo S, Frier RB, Campellone KG, 2023. Genomic instability caused by Arp2/3 complex inactivation results in micronucleus biogenesis and cellular senescence. PLOS Genet 19, e1010045. 10.1371/journal.pgen.1010045. 36706133 PMC9907832 · DOI
  112. Hansen M, Rubinsztein DC, Walker DW, 2018. Autophagy as a promoter of longevity: insights from model organisms. Nat. Rev. Mol. Cell Biol. 19, 579– 593. 10.1038/s41580-018-0033-y. 30006559 PMC6424591 · DOI · Paper page
  113. Hao Y-H, Doyle JM, Ramanathan S, Gomez TS, Jia D, Xu M, Chen ZJ, Billadeau DD, Rosen MK, Potts PR, 2013. Regulation of WASH-Dependent Actin Polymerization and Protein Trafficking by Ubiquitination. Cell 152, 1051– 1064. 10.1016/j.cell.2013.01.051. 23452853 PMC3640276 · DOI
  114. Haupt Y, Maya R, Kazaz A, Oren M, 1997. Mdm2 promotes the rapid degradation of p53. Nature 387, 296– 299. 10.1038/387296a0. 9153395 · DOI · Paper page
  115. Helgeson LA, Nolen BJ, 2013. Mechanism of synergistic activation of Arp2/3 complex by cortactin and N-WASP. eLife 2, e00884. 10.7554/eLife.00884. 24015358 PMC3762189 · DOI
  116. Hipp MS, Kasturi P, Hartl FU, 2019. The proteostasis network and its decline in ageing. Nat. Rev. Mol. Cell Biol. 20, 421– 435. 10.1038/s41580-019-0101-y. 30733602 · DOI · Paper page
  117. Hoffmann L, Waclawczyk MS, Tang S, Hanschmann E-M, Gellert M, Rust MB, Culmsee C, 2021. Cofilin1 oxidation links oxidative distress to mitochondrial demise and neuronal cell death. Cell Death Dis. 12, 953. 10.1038/s41419-021-04242-1. 34657120 PMC8520533 · DOI
  118. Hofmann WA, Stojiljkovic L, Fuchsova B, Vargas GM, Mavrommatis E, Philimonenko V, Kysela K, Goodrich JA, Lessard JL, Hope TJ, Hozak P, de Lanerolle P, 2004. Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat. Cell Biol. 6, 1094– 1101. 10.1038/ncb1182. 15502823 · DOI · Paper page
  119. Hsieh CW, Yang WY, 2019. Omegasome-proximal PtdIns(4,5)P2 couples F-actin mediated mitoaggregate disassembly with autophagosome formation during mitophagy. Nat. Commun. 10. 10.1038/S41467-019-08924-5. PMC6393429 30814505 · DOI
  120. Hu J, Zhang H, Li J, Jiang X, Zhang Y, Wu Q, Shen L, Shi J, Gao N, 2020. ROCK1 activation-mediated mitochondrial translocation of Drp1 and cofilin are required for arnidiol-induced mitochondrial fission and apoptosis. J. Exp. Clin. Cancer Res. CR 39, 37. 10.1186/s13046-020-01545-7. 32075676 PMC7031977 · DOI
  121. Hu P, Wu S, Hernandez N, 2004. A role for beta-actin in RNA polymerase III transcription. Genes Dev. 18, 3010– 3015. 10.1101/gad.1250804. 15574586 PMC535912 · DOI
  122. Hu Q, Wu D, Chen W, Yan Z, Yan C, He T, Liang Q, Shi Y, 2014, Molecular determinants of caspase-9 activation by the Apaf-1 apoptosome. Proc. Natl. Acad. Sci. U. S. A. 111, 16254– 16261. 10.1073/pnas.1418000111. 25313070 PMC4246342 · DOI
  123. Hu X, Mullins RD, 2019. LC3 and STRAP regulate actin filament assembly by JMY during autophagosome formation. J. Cell Biol. 218, 251– 266. 10.1083/jcb.201802157. 30420355 PMC6314544 · DOI
  124. Huang J, Birmingham CL, Shahnazari S, Shiu J, Zheng YT, Smith AC, Campellone KG, Heo WD, Gruenheid S, Meyer T, Welch MD, Ktistakis NT, Kim PK, Klionsky DJ, Brumell JH, 2011. Antibacterial autophagy occurs at PI (3)P-enriched domains of the endoplasmic reticulum and requires Rab1 GTPase. Autophagy 7, 17– 26. 10.4161/auto.7.1.13840. 20980813 PMC3039730 · DOI
  125. Huang X, Ding L, Pan R, Ma P-F, Cheng P-P, Zhang C-H, Shen Y-T, Xu L, Liu Y, He X-Q, Qi Z-Q, Wang H-L, 2013. WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes. Histochem. Cell Biol. 139, 525– 534. 10.1007/s00418-012-1051-z. 23160625 · DOI
  126. Innocenti M, 2018. New insights into the formation and the function of lamellipodia and ruffles in mesenchymal cell migration. Cell Adhes. Migr. 12, 401– 416. 10.1080/19336918.2018.1448352. PMC6363039 29513145 · DOI
  127. Innocenti M, Gerboth S, Rottner K, Lai FPL, Hertzog M, Stradal TEB, Frittoli E, Didry D, Polo S, Disanza A, Benesch S, Di Fiore PP, Carlier M-F, Scita G, 2005. Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes. Nat. Cell Biol. 7, 969– 976. 10.1038/ncb1304. 16155590 · DOI
  128. Inoue D, Obino D, Pineau J, Farina F, Gaillard J, Guerin C, Blanchoin L, Lennon-Duménil A-M, Théry M, 2019. Actin filaments regulate microtubule growth at the centrosome. EMBO J. 38, e99630 10.15252/embj.201899630. 30902847 PMC6545561 · DOI
  129. Ismail AM, Padrick SB, Chen B, Umetani J, Rosen MK, 2009. The WAVE Regulatory Complex is Inhibited. Nat. Struct. Mol. Biol. 16, 561. 10.1038/NSMB.1587. 19363480 PMC2716658 · DOI
  130. Ito Y, Carss KJ, Duarte ST, Hartley T, Keren B, Kurian MA, Marey I, Charles P, Mendonça C, Nava C, Pfundt R, Sanchis-Juan A, van Bokhoven H, van Essen A, van Ravenswaaij-Arts C, Kernohan KM, Dyack S, K.D., Raymond FL, NIHR BioResourceCare4Rare Canada Consortium, Boycott, 2018. De Novo Truncating Mutations in WASF1 Cause Intellectual Disability with Seizures. Am. J. Hum. Genet 103, 144– 153 10.1016/j.ajhg.2018.06.001. 29961568 PMC6037130 · DOI · Paper page
  131. Jarsch IK, Gadsby JR, Nuccitelli A, Mason J, Shimo H, Pilloux L, Marzook B, Mulvey CM, Dobramysl U, Bradshaw CR, Lilley KS, Hayward RD, Vaughan TJ, Dobson CL, Gallop JL, 2020. A direct role for SNX9 in the biogenesis of filopodia. J. Cell Biol. 219, e201909178 10.1083/jcb.201909178. 32328641 PMC7147113 · DOI
  132. Ji CH, Kwon YT, 2017. Crosstalk and interplay between the ubiquitin-proteasome system and autophagy. Mol. Cells 40, 441– 449. 10.14348/molcells.2017.0115. 28743182 PMC5547213 · DOI
  133. Ji W, Hatch AL, Merrill RA, Strack S, Higgs HN, 2015. Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites. eLife 4, e11553. 10.7554/eLife.11553. 26609810 PMC4755738 · DOI
  134. Jia D, Gomez TS, Metlagel Z, Umetani J, Otwinowski Z, Rosen MK, Billadeau DD, 2010, WASH and WAVE actin regulators of the Wiskott–Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes. Proc. Natl. Acad. Sci. 107, 10442– 10447. 10.1073/pnas.0913293107. 20498093 PMC2890800 · DOI
  135. Jin M, Shirazinejad C, Wang B, Yan A, Schöneberg J, Upadhyayula S, Xu K, Drubin DG, 2022. Branched actin networks are organized for asymmetric force production during clathrin-mediated endocytosis in mammalian cells. Nat. Commun. 13, 3578. 10.1038/s41467-022-31207-5. 35732852 PMC9217951 · DOI
  136. Jinks RN, Puffenberger EG, Baple E, Harding B, Crino P, Fogo AB, Wenger O, Xin B, Koehler AE, McGlincy MH, Provencher MM, Smith JD, Tran L, Al Turki S, Chioza BA, Cross H, Harlalka GV, Hurles ME, Maroofian R, Heaps AD, Morton MC, Stempak L, Hildebrandt F, Sadowski CE, Zaritsky J, Campellone K, Morton DH, Wang H, Crosby A, Strauss KA, 2015. Recessive nephrocerebellar syndrome on the Galloway-Mowat syndrome spectrum is caused by homozygous protein-truncating mutations of WDR73. Brain 138, 2173– 2190. 10.1093/brain/awv153. 26070982 PMC4511861 · DOI
  137. Jo Y-J, Kwon J, Jin Z-L, Namgoong S, Kwon T, Yoon S-B, Lee D-H, Kim J-S, Kim N-H, 2021. WHAMM is essential for spindle formation and spindle actin polymerization in maturing mouse oocytes. Cell Cycle 20, 225– 235. 10.1080/15384101.2020.1867791. 33397186 PMC7889088 · DOI
  138. Jost PJ, Grabow S, Gray D, McKenzie MD, Nachbur U, Huang DCS, Bouillet P, Thomas HE, Borner C, Silke J, Strasser A, Kaufmann T, 2009. XIAP discriminates between type I and type II FAS-induced apoptosis. Nature 460, 1035– 1039. 10.1038/nature08229. 19626005 PMC2956120 · DOI · Paper page
  139. Juin A, Spence HJ, Martin KJ, McGhee E, Neilson M, Cutiongco MFA, Gadegaard N, Mackay G, Fort L, Lilla S, Kalna G, Thomason P, Koh YWH, Norman JC, Insall RH, Machesky LM, 2019. N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell Metastasis. Dev. Cell 51, 431. 10.1016/J.DEVCEL.2019.09.018. 31668663 PMC6863394 · DOI
  140. Kabrawala S, Zimmer MD, Campellone KG, 2020. WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility. PLOS Genet 16, e1008694. 10.1371/JOURNAL.PGEN.1008694. 32196488 PMC7112243 · DOI
  141. Kadzik RS, Homa KE, Kovar DR, 2020. F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation. Annu. Rev. Cell Dev. Biol. 36, 35– 60. 10.1146/annurev-cellbio-032320-094706. 33021819 PMC8675537 · DOI · Paper page
  142. Kahr WHA, Pluthero FG, Elkadri A, Warner N, Drobac M, Chen CH, Lo RW, Li L, Li R, Li Q, Thoeni C, Pan J, Leung G, Lara-Corrales I, Murchie R, Cutz E, Laxer RM, Upton J, Roifman CM, Yeung RSM, Brumell JH, Muise AM, 2017. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat. Commun. 8 10.1038/NCOMMS14816. PMC5382316 28368018 · DOI
  143. Kaksonen M, Roux A, 2018. Mechanisms of clathrin-mediated endocytosis. Nat. Rev. Mol. Cell Biol. 19, 313– 326. 10.1038/nrm.2017.132. 29410531 · DOI · Paper page
  144. Kakuta S, Yamaguchi J, Suzuki C, Sasaki M, Kazuno S, Uchiyama Y, 2017. Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation. FASEB J. 31, 3757– 3773. 10.1096/fj.201601052R. 28522593 · DOI
  145. Kalkavan H, Green DR, 2018. MOMP, cell suicide as a BCL-2 family business. Cell Death Differ. 25, 46– 55. 10.1038/cdd.2017.179. 29053143 PMC5729535 · DOI
  146. Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM, 2010. Nanoscale architecture of integrin-based cell adhesions. Nature 468, 580– 584. 10.1038/nature09621. 21107430 PMC3046339 · DOI
  147. Kang R, Tang D, Yu Y, Wang Z, Hu T, Wang H, Cao L, 2010. WAVE1 regulates Bcl-2 localization and phosphorylation in leukemia cells. Leukemia 24, 177– 186. 10.1038/leu.2009.224. 19890377 PMC2866426 · DOI
  148. Kapoor P, Shen X, 2014. Mechanisms of nuclear actin in chromatin-remodeling complexes. Trends Cell Biol. 24, 238– 246. 10.1016/j.tcb.2013.10.007. 24246764 PMC3995414 · DOI · Paper page
  149. Kast DJ, Zajac AL, Holzbaur ELF, Ostap EM, Dominguez R, 2015. WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism. Curr. Biol. 25, 1791– 1797. 10.1016/j.cub.2015.05.042. 26096974 PMC4489997 · DOI
  150. Kastenhuber ER, Lowe SW, 2017. Putting p53 in Context. Cell 170, 1062– 1078. 10.1016/j.cell.2017.08.028. 28886379 PMC5743327 · DOI · Paper page
  151. Kaushik S, Cuervo AM, 2018. The coming of age of chaperone-mediated autophagy. Nat. Rev. Mol. Cell Biol. 19, 365– 381. 10.1038/s41580-018-0001-6. 29626215 PMC6399518 · DOI
  152. Kay RR, 2021. Macropinocytosis: Biology and mechanisms. Cells Dev. 168, 203713 10.1016/J.CDEV.2021.203713. 34175511 · DOI
  153. Kayalar C, Ord T, Testa MP, Zhong LT, Bredesen DE, 1996, Cleavage of actin by interleukin 1 beta-converting enzyme to reverse DNase I inhibition. Proc. Natl. Acad. Sci. U. S. A. 93, 2234– 2238. 10.1073/pnas.93.5.2234. 8700913 PMC39941 · DOI
  154. Kerr JF, Wyllie AH, Currie AR, 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26, 239– 257. 10.1038/bjc.1972.33. 4561027 PMC2008650 · DOI
  155. Killackey SA, Philpott DJ, Girardin SE, 2020. Mitophagy pathways in health and disease. J. Cell Biol. 219, e202004029 10.1083/JCB.202004029. 32926082 PMC7594502 · DOI
  156. Kim H-E, Du F, Fang M, Wang X, 2005. Formation of apoptosome is initiated by cytochrome c-induced dATP hydrolysis and subsequent nucleotide exchange on Apaf-1. Proc. Natl. Acad. Sci. U. S. A. 102, 17545– 17550. 10.1073/pnas.0507900102. 16251271 PMC1266161 · DOI
  157. Kim IH, Kim N, Kim S, Toda K, Catavero CM, Courtland JL, Yin HH, Soderling SH, 2020. Dysregulation of the Synaptic Cytoskeleton in the PFC Drives Neural Circuit Pathology, Leading to Social Dysfunction. Cell Rep. 32, 107965 10.1016/j.celrep.2020.107965. 32726629 PMC8000056 · DOI
  158. King VL, Leclair NK, Coulter AM, Campellone KG, 2021. The actin nucleation factors JMY and WHAMM enable a rapid Arp2/3 complex-mediated intrinsic pathway of apoptosis. PLoS Genet 17, e1009512. 10.1371/journal.pgen.1009512. 33872315 PMC8084344 · DOI
  159. Kirkin V, Lamark T, Sou Y-S, Bjørkøy G, Nunn JL, Bruun J-A, Shvets E, Mcewan DG, Clausen TH, Wild P, Bilusic I, Theurillat J-P, Øvervatn A, Ishii T, Elazar Z, Komatsu M, Dikic I, Johansen T, 2009. A Role for NBR1 in Autophagosomal Degradation of Ubiquitinated Substrates. Mol. Cell 33, 505– 516. 10.1016/j.molcel.2009.01.020. 19250911 · DOI
  160. Kitada T, Asakawa S, Hattori N, Matsumine H, Yamamura Y, Minoshima S, Yokochi M, Mizuno Y, Shimizu N, 1998. Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature 392, 605– 608. 10.1038/33416. 9560156 · DOI · Paper page
  161. Klamt F, Zdanov S, Levine RL, Pariser A, Zhang Y, Zhang B, Yu L-R, Veenstra TD, Shacter E, 2009. Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin. Nat. Cell Biol. 11, 1241– 1246. 10.1038/ncb1968. 19734890 PMC3393095 · DOI
  162. Kocks C, Gouin E, Tabouret M, Berche P, Ohayon H, Cossart P, 1992. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell 68, 521– 531. 10.1016/0092-8674(92)90188-I. 1739966 · DOI · Paper page
  163. Kollmar M, Lbik D, Enge S, 2012. Evolution of the eukaryotic ARP2/3 activators of the WASP family: WASP, WAVE, WASH, and WHAMM, and the proposed new family members WAWH and WAML. BMC Res. Notes 5, 88. 10.1186/1756-0500-5-88. 22316129 PMC3298513 · DOI
  164. Korobova F, Ramabhadran V, Higgs HN, 2013. An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2. Science 339, 464– 467. 10.1126/science.1228360. 23349293 PMC3843506 · DOI · Paper page
  165. Korobova F, Gauvin TJ, Higgs HN, 2014. A role for myosin II in mammalian mitochondrial fission. Curr. Biol. CB 24, 409– 414. 10.1016/j.cub.2013.12.032. 24485837 PMC3958938 · DOI
  166. Korolchuk VI, Menzies FM, Rubinsztein DC, 2010. Mechanisms of cross-talk between the ubiquitin-proteasome and autophagy-lysosome systems. FEBS Lett. 584, 1393– 1398. 10.1016/j.febslet.2009.12.047. 20040365 · DOI
  167. King VL, Campellone KG, 2023, F-actin-rich territories coordinate apoptosome assembly and caspase activation during DNA damage-induced intrinsic apoptosis. Mol Biol Cell in press. PMC10162420 36920061
  168. Koronakis V, Hume PJ, Humphreys D, Liu T, Hørning O, Jensen ON, McGhie EJ, 2011, WAVE regulatory complex activation by cooperating GTPases Arf and Rac1. Proc. Natl. Acad. Sci. U. S. A. 108, 14449– 14454. 10.1073/PNAS.1107666108. 21844371 PMC3167530 · DOI · Paper page
  169. Kothakota S, Azuma T, Reinhard C, Klippel A, Tang J, Chu K, McGarry TJ, Kirschner MW, Koths K, Kwiatkowski DJ, Williams LT, 1997. Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis. Science 278, 294– 298. 10.1126/science.278.5336.294. 9323209 · DOI
  170. Kramer DA, Piper HK, Chen B, 2022. WASP family proteins: Molecular mechanisms and implications in human disease. Eur. J. Cell Biol. 101, 151244 10.1016/J.EJCB.2022.151244. 35667337 PMC9357188 · DOI · Paper page
  171. Kruppa AJ, Kishi-Itakura C, Masters TA, Rorbach JE, Grice GL, Kendrick-Jones J, Nathan JA, Minczuk M, Buss F, 2018. Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria. Dev. Cell 44 (484–499), e6. 10.1016/j.devcel.2018.01.007. PMC5932465 29398621 · DOI
  172. Kubbutat MH, Jones SN, Vousden KH, 1997. Regulation of p53 stability by Mdm2. Nature 387, 299– 303. 10.1038/387299a0. 9153396 · DOI · Paper page
  173. Kuijpers TW, Tool ATJ, van der Bijl I, de Boer M, van Houdt M, de Cuyper IM, Roos D, van Alphen F, van Leeuwen K, Cambridge EL, Arends MJ, Dougan G, Clare S, Ramirez-Solis R, Pals ST, Adams DJ, Meijer AB, van den Berg TK, 2017. Combined immunodeficiency with severe inflammation and allergy caused by ARPC1B deficiency. e10 J. Allergy Clin. Immunol. 140, 273– 277. 10.1016/J.JACI.2016.09.061. 27965109 · DOI
  174. Kumar S, Xu J, Perkins C, Guo F, Snapper S, Finkelman FD, Zheng Y, Filippi M-D, 2012. Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules. Blood 120, 3563– 3574. 10.1182/blood-2012-04-426981. 22932798 PMC3482864 · DOI
  175. Kusano H, Shimizu S, Koya RC, Fujita H, Kamada S, Kuzumaki N, Tsujimoto Y, 2000. Human gelsolin prevents apoptosis by inhibiting apoptotic mitochondrial changes via closing VDAC. Oncogene 19, 4807– 4814. 10.1038/sj.onc.1203868. 11039896 · DOI
  176. Lamark T, Johansen T, 2021. Mechanisms of Selective Autophagy. Annu. Rev. Cell Dev. Biol. 37, 143– 169. 10.1146/ANNUREV-CELLBIO-120219-035530. 34152791 · DOI · Paper page
  177. Lappalainen P, Kotila T, Jégou A, Romet-Lemonne G, 2022. Biochemical and mechanical regulation of actin dynamics. Nat. Rev. Mol. Cell Biol. 23, 836– 852. 10.1038/s41580-022-00508-4. 35918536 · DOI
  178. Lartigue L, Medina C, Schembri L, Chabert P, Zanese M, Tomasello F, Dalibart R, Thoraval D, Crouzet M, Ichas F, De Giorgi F, 2008. An intracellular wave of cytochrome c propagates and precedes Bax redistribution during apoptosis. J. Cell Sci. 121, 3515– 3523. 10.1242/jcs.029587. 18840646 · DOI
  179. Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris DP, Fogel AI, Youle RJ, 2015. The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524, 309– 314. 10.1038/nature14893. 26266977 PMC5018156 · DOI · Paper page
  180. LeClaire LL, Baumgartner M, Iwasa JH, Mullins RD, Barber DL, 2008. Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments. J. Cell Biol. 182, 647– 654. 10.1083/jcb.200802145. 18725535 PMC2518704 · DOI
  181. Lee K, Gallop JL, Rambani K, Kirschner MW, 2010. Self-Assembly of Filopodia-Like Structures on Supported Lipid Bilayers. Science 329, 1341– 1345. 10.1126/science.1191710. 20829485 PMC2982780 · DOI
  182. Lee PP, Lobato-Márquez D, Pramanik N, Sirianni A, Daza-Cajigal V, Rivers E, Cavazza A, Bouma G, Moulding D, Hultenby K, Westerberg LS, Hollinshead M, Lau YL, Burns SO, Mostowy S, Bajaj-Elliott M, Thrasher AJ, 2017. Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells. Nat. Commun. 8. 10.1038/S41467-017-01676-0. PMC5691069 29146903 · DOI
  183. Legg JA, Bompard G, Dawson J, Morris HL, Andrew N, Cooper L, Johnston SA, Tramountanis G, Machesky LM, 2007. N-WASP Involvement in Dorsal Ruffle Formation in Mouse Embryonic Fibroblasts. Mol. Biol. Cell 18, 678. 10.1091/MBC.E06-06-0569. 17182853 PMC1783773 · DOI
  184. Levkau B, Herren B, Koyama H, Ross R, Raines EW, 1998. Caspase-mediated cleavage of focal adhesion kinase pp125FAK and disassembly of focal adhesions in human endothelial cell apoptosis. J. Exp. Med 187, 579– 586. 10.1084/jem.187.4.579. 9463408 PMC2212148 · DOI
  185. Li H, Zhu H, Xu CJ, Yuan J, 1998. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 94, 491– 501. 10.1016/s0092-8674(00)81590-1. 9727492 · DOI · Paper page
  186. Li R, Zhu J, 2022. Effects of aneuploidy on cell behaviour and function. Nat. Rev. Mol. Cell Biol. 23, 250– 265. 10.1038/s41580-021-00436-9. 34987171 · DOI
  187. Li S, Xu S, Roelofs BA, Boyman L, Lederer WJ, Sesaki H, Karbowski M, 2015. Transient assembly of F-actin on the outer mitochondrial membrane contributes to mitochondrial fission. J. Cell Biol. 208, 109– 123. 10.1083/jcb.201404050. 25547155 PMC4284235 · DOI
  188. Li Y, Zhou M, Hu Q, Bai X-C, Huang W, Scheres SHW, Shi Y, 2017, Mechanistic insights into caspase-9 activation by the structure of the apoptosome holoenzyme. Proc. Natl. Acad. Sci. U. S. A. 114, 1542– 1547. 10.1073/pnas.1620626114. 28143931 PMC5320974 · DOI
  189. Lim KB, Bu W, Goh WI, Koh E, Ong SH, Pawson T, Sudhaharan T, Ahmed S, 2008. The Cdc42 effector IRSp53 generates filopodia by coupling membrane protrusion with actin dynamics. J. Biol. Chem. 283, 20454– 20472. 10.1074/jbc.M710185200. 18448434 · DOI
  190. Linardopoulou EV, Parghi SS, Friedman C, Osborn GE, Parkhurst SM, Trask BJ, 2007. Human Subtelomeric WASH Genes Encode a New Subclass of the WASP Family. PLoS Genet 3, 2477– 2485. 10.1371/JOURNAL.PGEN.0030237. PMC2151093 18159949 · DOI
  191. Linder S, 2009. Invadosomes at a glance. J. Cell Sci. 122, 3009– 3013. 10.1242/jcs.032631. 19692587 · DOI
  192. Liu A, Kage F, Higgs HN, 2021. Mff oligomerization is required for Drp1 activation and synergy with actin filaments during mitochondrial division. ar5 Mol. Biol. Cell 32. 10.1091/mbc.E21-04-0224. PMC8684745 34347505 · DOI
  193. Liu J, Wang Q-C, Wang F, Duan X, Dai X-X, Wang T, Liu H-L, Cui X-S, Kim N-H, Sun S-C, 2012. Nucleation promoting factors regulate the expression and localization of Arp2/3 complex during meiosis of mouse oocytes. PloS One 7, e52277. 10.1371/journal.pone.0052277. 23272233 PMC3525642 · DOI
  194. Liu M, Zhang J, Pinder BD, Liu Q, Wang D, Yao H, Gao Y, Toker A, Gao J, Peterson A, Qu J, Siminovitch KA, 2021. WAVE2 suppresses mTOR activation to maintain T cell homeostasis and prevent autoimmunity. Science 371, eaaz4544. 10.1126/science.aaz4544. 33766857 · DOI · Paper page
  195. Livneh I, Cohen-Kaplan V, Cohen-Rosenzweig C, Avni N, Ciechanover A, 2016. The life cycle of the 26S proteasome: From birth, through regulation and function, and onto its death. Cell Res 26, 869– 885. 10.1038/cr.2016.86. 27444871 PMC4973335 · DOI
  196. Lommel S, Benesch S, Rottner K, Franz T, Wehland J, Kühn R, 2001. Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep. 2, 850– 857. 10.1093/embo-reports/kve197. 11559594 PMC1084051 · DOI
  197. Luciani F, Matarrese P, Giammarioli AM, Lugini L, Lozupone F, Federici C, Iessi E, Malorni W, Fais S, 2004. CD95/phosphorylated ezrin association underlies HIV-1 GP120/IL-2-induced susceptibility to CD95(APO-1/Fas)-mediated apoptosis of human resting CD4(+)T lymphocytes. Cell Death Differ. 11, 574– 582. 10.1038/sj.cdd.4401374. 14739941 · DOI
  198. Luna A, Matas OB, Martínez-Menárguez JA, Mato E, Durán JM, Ballesta J, Way M, Egea G, 2002. Regulation of Protein Transport from the Golgi Complex to the Endoplasmic Reticulum by CDC42 and N-WASP. Mol. Biol. Cell 13, 866– 879. 10.1091/mbc.01-12-0579. 11907268 PMC99605 · DOI
  199. Luo X, Budihardjo I, Zou H, Slaughter C, Wang X, 1998. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94, 481– 490. 10.1016/s0092-8674(00)81589-5. 9727491 · DOI · Paper page
  200. Lusk CP, King MC, 2017. The nucleus: keeping it together by keeping it apart. Curr. Opin. Cell Biol. 44, 44– 50. 10.1016/j.ceb.2017.02.001. 28236735 PMC5403602 · DOI

200 of 383 shown

Cited by

Sorted by citations

Authors
Campellone, K. G., Lebek, N. M., & King, V. L.
Affiliations
University of Connecticut (US)
Corresponding author
Campellone, Kenneth G.
ORCID
Venue
European Journal of Cell Biology
Publisher
Elsevier BV
Published
2023-06
Volume
102
Issue
2
Pages
151301
ISSN
0171-9335 (ISSN-L)
DOI
10.1016/j.ejcb.2023.151301
PMC
PMC10330494
OpenAlex
W4322766066
Cited by
50
References
13
Citations by year
2026: 16 · 2025: 22 · 2024: 8 · 2023: 4
FWCI
6.98
Type
journal article
Language
en
Open access
Licence
Locations
  • European Journal of Cell Biology (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health
  • National Institute on Aging (K02AG050774)
  • National Institute of General Medical Sciences (R01GM107441)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
Arp2/3 complex, WASP family proteins, actin nucleation, actin cytoskeleton, autophagy, apoptosis, chromatin dynamics, DNA repair, cytoskeletal rearrangement
MeSH terms
Actins, Animals, Cytoskeleton, Actin Cytoskeleton, Wiskott-Aldrich Syndrome Protein Family, Wiskott-Aldrich Syndrome Protein, Actin-Related Protein 2-3 Complex, Actin-Related Protein 3

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