Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
Open accessAbstract
Sestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXD mice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model.
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Aerobic exercise can suppress skeletal muscle atrophy by improving functional status of autophagy, as confirmed by expression levels of Atg5, Atg7, p62, and LC3-II in aging rat model.
“Moreover, aerobic exercise can suppress skeletal muscle atrophy by improving the functional status of autophagy, as confirmed by the expression levels of multiple autophagy-related biomarkers, including Atg5, Atg7, p62, and LC3-II in the aging rat model [35].”
From Lifelong Aerobic Exercise Alleviates Sarcopenia by Activating Autophagy and Inhibiting Protein Degradation via the AMPK/PGC-1α Signaling Pathway (2021) · cites this paper as [35]
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- Authors
- Lenhare, L., Crisol, B. M., Silva, V. R. R., Katashima, C. K., Cordeiro, A. V., Pereira, K. D., Luchessi, A. D., da Silva, A. S. R., Cintra, D. E., Moura, L. P., Pauli, J. R., & Ropelle, E. R.
- Affiliations
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Corresponding author
- Ropelle, Eduardo R.
- ORCID
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 97
- Pages
- 17-21
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W2734602785
- Cited by
- 79
- References
- 17
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 6 · 2023: 10 · 2022: 11 · 2021: 12 · 2020: 10 · 2019: 9 · 2018: 8 · 2017: 3
- FWCI
- 6.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (publishedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2016/01089-3)
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- Sestrin2, autophagy, acute aerobic exercise, skeletal muscle, aging, swimming training, gastrocnemius, insulin sensitivity, ULK1 phosphorylation, ATG5, ATG7, p62, LC3-II, BXD mice, triglycerides, fasting blood glucose, weight gain
- MeSH terms
- Sestrins, Aging, Animals, Autophagy, Gene Expression Regulation, Glucose, Insulin, Insulin Resistance, Male, Mice, Inbred C57BL, Nuclear Proteins, Peroxidases, Phosphorylation, Physical Conditioning, Animal, RNA, Messenger, Swimming, Biomarkers, Muscle, Skeletal, Mice, AMP-Activated Protein Kinases
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