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The microRNAs Let7c and miR21 are recognized by neuronal Toll-like receptor 7 to restrict dendritic growth of neurons

Liu, Hsin-Yu, Huang, Chiao-Ming, Hung, Yun-Fen, Hsueh, Yi-Ping·2015·Experimental Neurology
DOI 10.1016/j.expneurol.2015.04.011
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Abstract

Inflammatory responses are known to play critical roles in the regulation of neurodevelopment and neurodegeneration. Although microglial cells are recognized as professional immune cells in brains, recent evidence suggests that neurons also express important receptors and regulators of innate immunity, including Toll-like receptor 7 (TLR7), which is a receptor for single-stranded RNAs (ssRNAs). Here, we report that neuronal TLR7 recognizes endogenous ligands such as the miRNAs Let7c and miR21 and plays a negative role in controlling neuronal growth in a cell-autonomous manner. We show here that hippocampal CA1 neurons in Tlr7(-/Y) mice had more complex dendritic arbors compared with those of wild-type littermates at postnatal (P) day 7, but not at P21. This observation strengthens a role of TLR7 in restricting neuronal growth during development. In cultured neurons, transient knockdown of Tlr7 promoted axonal and dendritic growth, supporting the cell-autonomous effect of TLR7 on neuronal growth. We observed perceptible levels of Let7c and miR21 in the exosomes of the neuronal cultures as well as in developing brains. Treatment with Let7c and miR21 restricted dendritic growth of wild-type neurons but not Tlr7(-/-) neurons. Our study suggests that neuronal TLR7 is activated by endogenous ligands and thus regulates neuronal morphology. Neuronal innate immune responses may influence neurodevelopment and neurodegeneration through the regulation of neuronal morphology.

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Research works printed but not linked: 48

  1. Alexopoulou, 2001, Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3, Nature, 413, 732, 10.1038/35099560 · DOI · Paper page
  2. Anderson, 1985, Establishment of dorsal–ventral polarity in the Drosophila embryo: genetic studies on the role of the Toll gene product, Cell, 42, 779, 10.1016/0092-8674(85)90274-0 · DOI · Paper page
  3. Baker, 2013, Toll-like receptor 7 suppresses virus replication in neurons but does not affect viral pathogenesis in a mouse model of Langat virus infection, J. Gen. Virol., 94, 336, 10.1099/vir.0.043984-0 · DOI
  4. Bareyre, 2005, Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury, Nat. Med., 11, 1355, 10.1038/nm1331 · DOI
  5. Bartel, 2009, MicroRNAs: target recognition and regulatory functions, Cell, 136, 215, 10.1016/j.cell.2009.01.002 · DOI · Paper page
  6. Beignon, 2005, Endocytosis of HIV-1 activates plasmacytoid dendritic cells via Toll-like receptor–viral RNA interactions, J. Clin. Invest., 115, 3265, 10.1172/JCI26032 · DOI
  7. Buffelli, 2003, Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition, Nature, 424, 430, 10.1038/nature01844 · DOI
  8. Cavassani, 2008, TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events, J. Exp. Med., 205, 2609, 10.1084/jem.20081370 · DOI
  9. Cheng, 2011, Self-amplifying autocrine actions of BDNF in axon development, Proc. Natl. Acad. Sci. U. S. A., 108, 18430, 10.1073/pnas.1115907108 · DOI
  10. Diebold, 2004, Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA, Science, 303, 1529, 10.1126/science.1093616 · DOI · Paper page
  11. Eiring, 2010, miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts, Cell, 140, 652, 10.1016/j.cell.2010.01.007 · DOI
  12. Escrevente, 2011, Interaction and uptake of exosomes by ovarian cancer cells, BMC Cancer, 11, 108, 10.1186/1471-2407-11-108 · DOI
  13. Fabbri, 2012, MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response, Proc. Natl. Acad. Sci. U. S. A., 109, E2110, 10.1073/pnas.1209414109 · DOI · Paper page
  14. Gerondakis, 2007, Regulating B-cell activation and survival in response to TLR signals, Immunol. Cell Biol., 85, 471, 10.1038/sj.icb.7100097 · DOI
  15. Gibson, 2008, Engagement of specific innate immune signaling pathways during Porphyromonas gingivalis induced chronic inflammation and atherosclerosis, Front. Biosci., 13, 2041, 10.2741/2822 · DOI
  16. Green, 2012, Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands, J. Biol. Chem., 287, 39789, 10.1074/jbc.M112.383000 · DOI
  17. Hayashi, 2001, The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5, Nature, 410, 1099, 10.1038/35074106 · DOI
  18. Heil, 2004, Species-specific recognition of single-stranded RNA via Toll-like receptor 7 and 8, Science, 303, 1526, 10.1126/science.1093620 · DOI · Paper page
  19. Hoshino, 1999, Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product, J. Immunol., 162, 3749, 10.4049/jimmunol.162.7.3749 · DOI · Paper page
  20. Jiang, 2006, High Ca2+-phosphate transfection efficiency in low-density neuronal cultures, Nat. Protoc., 1, 695, 10.1038/nprot.2006.86 · DOI
  21. Kaisho, 2006, Toll-like receptor function and signaling, J. Allergy Clin. Immunol., 117, 979, 10.1016/j.jaci.2006.02.023 · DOI
  22. Kariko, 2004, Inhibition of Toll-like receptor and cytokine signaling — a unifying theme in ischemic tolerance, J. Cereb. Blood Flow Metab., 24, 1288, 10.1097/01.WCB.0000145666.68576.71 · DOI
  23. Kumar, 2004, Innate immunity in the retina: Toll-like receptor (TLR) signaling in human retinal pigment epithelial cells, J. Neuroimmunol., 153, 7, 10.1016/j.jneuroim.2004.04.018 · DOI
  24. Lehmann, 2012, An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration, Nat. Neurosci., 15, 827, 10.1038/nn.3113 · DOI · Paper page
  25. Liu, 2002, Role of Toll-like receptors in changes in gene expression and NF-kappa B activation in mouse hepatocytes stimulated with lipopolysaccharide, Infect. Immun., 70, 3433, 10.1128/IAI.70.7.3433-3442.2002 · DOI · Paper page
  26. Liu, 2013, TLR7 negatively regulates dendrite outgrowth through the Myd88-c-Fos-IL-6 pathway, J. Neurosci., 33, 11479, 10.1523/JNEUROSCI.5566-12.2013 · DOI · Paper page
  27. Liu, 2014, Innate immune responses regulate morphogenesis and degeneration: roles of Toll-like receptors and Sarm1 in neurons, Neurosci. Bull., 30, 645, 10.1007/s12264-014-1445-5 · DOI
  28. Lund, 2004, Recognition of single-stranded RNA viruses by Toll-like receptor 7, Proc. Natl. Acad. Sci. U. S. A., 101, 5598, 10.1073/pnas.0400937101 · DOI
  29. Maiorano, 2009, Promotion of embryonic cortico-cerebral neuronogenesis by miR-124, Neural Dev., 4, 40, 10.1186/1749-8104-4-40 · DOI · Paper page
  30. Marz, 1996, Expression of interleukin-6 receptor (IL-6R) and gp130 mRNA in PC12 cells and sympathetic neurons: modulation by tumor necrosis factor alpha (TNF-alpha), Brain Res., 706, 71, 10.1016/0006-8993(95)01210-9 · DOI
  31. Olson, 2004, Microglia initiate central nervous system innate and adaptive immune responses through multiple TLRs, J. Immunol., 173, 3916, 10.4049/jimmunol.173.6.3916 · DOI · Paper page
  32. Park, 2014, Extracellular microRNAs activate nociceptor neurons to elicit pain via TLR7 and TRPA1, Neuron, 82, 47, 10.1016/j.neuron.2014.02.011 · DOI · Paper page
  33. Pearlman, 2008, Toll-like receptors at the ocular surface, Ocul. Surf., 6, 108, 10.1016/S1542-0124(12)70279-3 · DOI
  34. Peltier, 2008, Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues, RNA, 14, 844, 10.1261/rna.939908 · DOI · Paper page
  35. Porrero, 2010, Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice, Brain Res., 1345, 59, 10.1016/j.brainres.2010.05.061 · DOI
  36. Punnonen, 1998, At reduced temperature, endocytic membrane traffic is blocked in multivesicular carrier endosomes in rat cardiac myocytes, Eur. J. Cell Biol., 75, 344, 10.1016/S0171-9335(98)80067-8 · DOI
  37. Raines, 1998, Ribonuclease A, Chem. Rev., 98, 1045, 10.1021/cr960427h · DOI
  38. Reid, 2011, Circulating microRNAs: association with disease and potential use as biomarkers, Crit. Rev. Oncol. Hematol., 80, 193, 10.1016/j.critrevonc.2010.11.004 · DOI
  39. Sun, 2003, Inflammatory markers in matched plasma and cerebrospinal fluid from patients with Alzheimer's disease, Dement. Geriatr. Cogn. Disord., 16, 136, 10.1159/000071001 · DOI
  40. Sutmuller, 2007, Regulatory T cells and Toll-like receptors: regulating the regulators, Ann. Rheum. Dis., 66, iii91, 10.1136/ard.2007.078535 · DOI
  41. Valadi, 2007, Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells, Nat. Cell Biol., 9, 654, 10.1038/ncb1596 · DOI · Paper page
  42. Venkatasubramanian, 2013, The TRIPS (Toll-like receptors in immuno-inflammatory pathogenesis) Hypothesis: a novel postulate to understand schizophrenia, Prog. Neuropsychopharmacol. Biol. Psychiatry, 44, 301, 10.1016/j.pnpbp.2013.04.001 · DOI
  43. Welte, 2009, Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection, J. Gen. Virol., 90, 2660, 10.1099/vir.0.011783-0 · DOI
  44. Wu, 2013, Identification of TLR downstream pathways in stroke patients, Clin. Biochem., 46, 1058, 10.1016/j.clinbiochem.2013.05.059 · DOI
  45. Yang, 2005, Proinflammatory phenotype of vascular smooth muscle cells: role of efficient Toll-like receptor 4 signaling, Am. J. Physiol. Heart Circ. Physiol., 289, H1069, 10.1152/ajpheart.00143.2005 · DOI
  46. Yoshimoto, 2006, Roles of IL-18 in basophils and mast cells, Allergol. Int., 55, 105, 10.2332/allergolint.55.105 · DOI
  47. Yu, 2010, Endogenous Toll-like receptor ligands and their biological significance, J. Cell. Mol. Med., 14, 2592, 10.1111/j.1582-4934.2010.01127.x · DOI
  48. Zhao, 2009, PKCdelta regulates cortical radial migration by stabilizing the Cdk5 activator p35, Proc. Natl. Acad. Sci. U. S. A., 106, 21353, 10.1073/pnas.0812872106 · DOI

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Metadata

Authors
Liu, H.-Y., Huang, C.-M., Hung, Y.-F., & Hsueh, Y.-P.
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
2015-07
Volume
269
Pages
202-212
ISSN
0014-4886
DOI
10.1016/j.expneurol.2015.04.011
Cited by
59
References
3
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Funders
  • Academia Sinica (AS-103-TP-B05)
  • Ministry of Science and Technology (103-2321-B-001-002, 103-2811-B-001-072)
  • Y.-P. Hsueh (103-2321-B-001-018)

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