Cited by 75Antioxidant Activity and Oxidative StressNeutrophil, Myeloperoxidase and Oxidative MechanismsExercise and Physiological Responses

ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin

Marin, Douglas Popp, Bolin, Anaysa Paola, Macedo, Rita de Cássia Santos, Sampaio, Sandra Coccuzzo, Otton, Rosemari·2011·International Immunopharmacology

Abstract

Background Astaxanthin (ASTA) is a carotenoid which has powerful antioxidant, anti-tumor, anti-diabetic, anti-inflammatory and cardioprotective properties. The present study investigated the effect of daily ASTA intake on oxidative stress and the functional properties of neutrophils from alloxan-induced diabetic rats.Methods Neutrophils isolated from ASTA-fed rats (30days, 20mg ASTA/kg of body weight - BW) induced to diabetes by alloxan treatment (i.p. 75mg/BW) were assessed by: production of superoxide and hydrogen peroxide, nitric oxide, basal calcium release, oxidative damage (TBARS and carbonyls content), and activities of major antioxidant enzymes.Results Our results show that diabetes promotes a significant oxidative stress in neutrophils. The production of superoxide was significantly increased in neutrophils from diabetic rats and treatment with ASTA was not effective in reducing superoxide levels. At the same time, a reduction in the activity of total superoxide dismutase enzyme was observed, which was not restored after treatment with ASTA. At resting conditions, neutrophils have a higher basal production of hydrogen peroxide, which is enhanced following PMA-stimulation. Treatment with ASTA does not restore values to the basal levels. The indicators of oxidative damage to biomolecules showed that diabetic rats significantly increased the lipid and protein damage, but this change was reversed after treatment with ASTA.Conclusion Our results show that diabetes condition promotes a marked oxidative stress in neutrophils and treatment with ASTA for 30days at a dose of 20mg/kg of BW partially reverses those deleterious effects.

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Crossref · 10.1016/j.intimp.2010.10.013

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  • Administration of astaxanthin in alloxan-induced diabetic rats can partially reverse oxidative stress in neutrophils.

    “For instance, it has been shown that administration of astaxanthin in alloxan-induced diabetic rats can partially reverse oxidative stress in neutrophils (Marin et al., 2011).”

    From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Marin et al., 2011

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Authors
Marin, D. P., Bolin, A. P., Macedo, R. D. C. S., Sampaio, S. C., & Otton, R.
Affiliations
  • Universidade Cruzeiro do Sul (BR)
  • Instituto Butantan (BR)
Corresponding author
Otton, Rosemari
Venue
International Immunopharmacology
Publisher
Elsevier BV
Published
2011-01
Volume
11
Issue
1
Pages
103-109
ISSN
1567-5769 (ISSN-L)
OpenAlex
W2089790886
Cited by
75
References
56
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 3 · 2022: 1 · 2021: 8 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 8 · 2015: 5 · 2014: 6 · 2013: 3 · 2012: 15
FWCI
4.15
Type
journal article
Language
en
Open access
closed
Locations
  • International Immunopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
astaxanthin, neutrophils, reactive oxygen species, oxidative stress, diabetes mellitus, alloxan-induced diabetes, superoxide, hydrogen peroxide, nitric oxide, superoxide dismutase, lipid peroxidation, protein carbonylation, antioxidant enzymes, carotenoids
MeSH terms
Alloxan, Animals, Antioxidants, Diabetes Mellitus, Experimental, Male, Neutrophils, Nitric Oxide, Rats, Wistar, Reactive Oxygen Species, Oxidative Stress, Xanthophylls, Rats

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