Dose-response effects of marine omega-3 fatty acids on apolipoproteins, apolipoprotein-defined lipoprotein subclasses, and Lp-PLA2 in individuals with moderate hypertriglyceridemia
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Background Apolipoprotein (apo) distribution and lipoprotein (Lp)-associated markers of inflammation, such as lipoprotein-associated phospholipase A2 (Lp-PLA2), influence the atherogenicity of circulating lipids and lipoproteins. Little evidence exists regarding the dose-response effects of the marine omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on apos, apo-defined Lps, and Lp-PLA2.Objective The purpose of this study was to compare the effects of 0, 0.85, and 3.4 g/d of EPA + DHA on Lp-PLA2 mass and activity in individuals with moderate hypertriglyceridemia. We also measured effects on concentrations of apoAI, apoAII, apoB, apoC, apoD, and apoE-defined Lp subclasses.Methods The study was a randomized, doubleblind, crossover design with 8-week treatment periods and 6-week washout periods. During the 3 treatment periods, subjects (n = 25) received 0 g/d EPA + DHA, 0.85 g/d EPA + DHA (low dose), and 3.4 g/d EPA + DHA (high dose) in random order.Results apoB and apoC-III were significantly decreased by the high dose relative to placebo and low dose (P < .01), as was very low-density lipoprotein cholesterol (P < .005). The low dose had no effect on Lp outcomes compared with placebo. The high- and low-dose effects differed significantly for heparin-precipitated apoC-III, LpB, LpA-I, and apoB/apoA-I ratio (P < .05). There was a trend for a decreased Lp-PLA2 mass with the high dose (P = .1).Conclusion The effects of 3.4 g/d EPA + DHA on apoB and apoC-III may reduce atherosclerotic plaque progression in individuals with elevated triglycerides.
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Omega-3 supplementation and Lp-PLA2: Attention to nonindependent comparisons in meta-analysis
Zhao, J., & Yan, J. (2026) · Journal of clinical lipidology · doi:10.1016/j.jacl.2026.04.014
Simental‐Mendía, L. E., & Simental-Mendía, M. A. (2026) · Journal of clinical lipidology · doi:10.1016/j.jacl.2026.04.031
- Authors
- Skulas-Ray, A. C., Alaupovic, P., Kris-Etherton, P. M., & West, S. G.
- Affiliations
- Oklahoma Medical Research Foundation (US)
- Pennsylvania State University (US)
- Venue
- Journal of Clinical Lipidology
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 9
- Issue
- 3
- Pages
- 360-367
- ISSN
- 1876-4789 (ISSN-L)
- 1933-2874
- PMC
- PMC4677829
- OpenAlex
- W2066520896
- Cited by
- 30
- References
- 64
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 6 · 2018: 3 · 2017: 5 · 2016: 2 · 2015: 1
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of clinical lipidology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- GlaxoSmithKline
- National Center for Advancing Translational Sciences (UL1 TR000127)
- National Institutes of Health
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- omega-3 fatty acids, eicosapentaenoic acid, docosahexaenoic acid, hypertriglyceridemia, apolipoproteins, lipoprotein subclasses, lipoprotein-associated phospholipase A2, dose-response relationship, apolipoprotein B, apolipoprotein C-III, VLDL cholesterol, atherosclerosis, inflammation
- MeSH terms
- Apolipoproteins B, Docosahexaenoic Acids, Dose-Response Relationship, Drug, Double-Blind Method, Female, Humans, Male, Middle Aged, Eicosapentaenoic Acid, Apolipoprotein A-I, Cross-Over Studies, 1-Alkyl-2-acetylglycerophosphocholine Esterase, Apolipoprotein C-III
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