CoChat
Sign Up
Cited by 37Fatty Acid Research and HealthInflammatory mediators and NSAID effectsEdible Oils Quality and Analysis

Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males

Mullen, Anne, Moloney, Fiona, Nugent, Anne P., Doyle, Lorna, Cashman, Kevin D., Roche, Helen M.·2007·The Journal of Nutritional Biochemistry
DOI 10.1016/j.jnutbio.2006.12.008
Open this paper in CoChat — free account

Abstract

Conjugated linoleic acid (CLA) refers to geometric and positional isomers of linoleic acid. Animal studies have shown that CLA modulates the immune system and suggest that it may have a therapeutic role in inflammatory disorders. This double-blind placebo-controlled intervention trial investigated the effects of CLA supplementation on indices of immunity relating to cardiovascular disease (CVD) in a cohort of healthy middle-aged male volunteers. Subjects were randomly assigned to supplement their diet with 2.2 g 50:50 isomeric blend of cis 9, trans 11 (c9, t11)-CLA and trans 10, cis 12 (t10, c12)-CLA or placebo daily for 8 weeks. Interleukin (IL) 2, IL-10 and tumour necrosis factor (TNF) alpha were measured in the supernatant of cultured unstimulated and concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC) by ELISA. Serum IL-6 and plasma CRP were measured by ELISA and plasma fibrinogen by automated clotting assay. Gene expression was investigated by real-time RT-PCR. CLA supplementation significantly reduced Con A-stimulated PBMC IL-2 secretion (37.1%; P=.02). CLA supplementation had no significant effect on transcription of IL-2. CLA supplementation had no direct significant effects on PBMC TNFalpha or IL-10 secretion. Other inflammatory markers associated with CVD, including IL-6, CRP and fibrinogen, were not affected by CLA supplementation. This study showed that CLA supplementation reduced PBMC IL-2 secretion from Con A-stimulated PBMC but lacked effect on other markers of the human inflammatory response.

Integrity signals

Registry

Crossref · 10.1016/j.jnutbio.2006.12.008

Cites

78 of 78 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Observational studies generally report inverse correlations between Mediterranean diet and inflammatory markers in healthy persons.

    “Observational studies have examined the association of the Mediterranean diet with inflammatory markers in healthy persons (282,283,285) , and they generally report inverse correlations.”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [285]

  • In a Nurses' Health Study subsample, Mediterranean diet score was inversely associated with IL-6, CRP, sICAM-1, sVCAM-1, and sE-selectin, persisting after adjustment for CVD risk factors.

    “In a subsample of the Nurses' Health Study (285) , a Mediterranean diet index score was inversely associated with markers of inflammation (circulating IL-6 and CRP) as well as markers of endothelial dysfunction (the adhesion molecules sICAM-1, sVCAM-1 and soluble E-selectin (sE-selectin)); these associations persisted upon adjustment for traditional CVD risk factors.”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [285]

  • In the same study, HEI and DQI-R did not correlate with any inflammatory markers after confounder adjustment.

    “In contrast, the HEI and DQI-R did not correlate with any of the inflammatory markers when potential confounders were taken into consideration in the regression models (285) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [285]

  • In a subsample of 690 healthy women from the Nurses' Health Study, AHEI and alternate Mediterranean diet index scores were negatively associated with CRP, IL-6, sE-selectin and sICAM-1, and these associations persisted after adjustment for confounders including BMI.

    “The key findings were that the AHEI and alternate Mediterranean diet index scores were negatively associated with CRP, IL-6, sE-selectin and sICAM-1 concentrations, and that these associations persisted upon adjustment for potential confounding variables including BMI.”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [285]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Mullen, A., Moloney, F., Nugent, A. P., Doyle, L., Cashman, K. D., & Roche, H. M.
Affiliations
  • St. James's Hospital (IE)
  • University College Cork (IE)
Corresponding author
Roche, Helen M.
ORCID
Venue
The Journal of Nutritional Biochemistry
Publisher
Elsevier BV
Published
2007-10
Volume
18
Issue
10
Pages
658-666
ISSN
0955-2863 (ISSN-L)
DOI
10.1016/j.jnutbio.2006.12.008
OpenAlex
W1975816358
Cited by
37
References
77
Citations by year
2025: 2 · 2023: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 6
FWCI
3.71
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • The Journal of Nutritional Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (King's College London) (submittedVersion)
  • LSHTM Research Online (London School of Hygiene and Tropical Medicine) (submittedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Keywords
conjugated linoleic acid, dietary supplementation, interleukin-2, peripheral blood mononuclear cells, immunomodulation, cardiovascular disease, inflammation, IL-10, TNF-α, interleukin-6, C-reactive protein, fibrinogen, concanavalin A stimulation, gene expression
MeSH terms
Adult, Concanavalin A, Double-Blind Method, Humans, Interleukin-2, Leukocytes, Mononuclear, Male, Middle Aged, Tumor Necrosis Factor-alpha, Interleukin-10, Linoleic Acids, Conjugated

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review