Systemic distribution and tissue localizations of human 17beta-hydroxysteroid dehydrogenase type 12
Abstract
The 17beta-hydroxysteroid dehydrogenases (HSDs) are enzymes that catalyze the reduction of 17-ketosteroids or the oxidation of 17beta-hydroxysteroids. 17beta-HSD type 12, the most recently cloned member of this gene family, was classified into the 17beta-HSD family based on sequence homology, rather than steroid catalyzing activity. Meanwhile, it has been reported that 17beta-HSD type 12 may be involved in fatty acid synthesis. To better understand the role of 17beta-HSD type 12 in lipid metabolism, we determined the detailed systemic distribution and tissue localizations of 17beta-HSD type 12, which, due partly to the lack of antibodies, had not yet been studied. We carried out these investigations by quantitative reverse transcription (RT)-PCR, Northern blot analysis, and immunohistochemistry, using an antibody against 17beta-HSD type 12 that we have generated. 17beta-HSD type 12 is highly expressed in organs related to lipid metabolism such as liver, kidney, heart and skeletal muscle. 17beta-HSD type 12 is also detected in endocrine-related organs such as pancreas, pituitary gland, adrenal gland, testis and placenta, and in the gastrointestinal tract, which point to the possible involvement of 17beta-HSD type 12 in the regulation of lipid biosynthesis and steroid metabolism. These results support previous reports and solidify the possibility that 17beta-HSD type 12 may play critical roles in the physiological processes, such as fatty acid synthesis, in addition to the steroid metabolism.
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17βHSD2 is found mostly in sebaceous glands of non-acne-prone areas compared with facial skin.
“17βHSD2 (which can inactivate potent androgens) and was found mostly in sebaceous glands of non-acne prone areas in comparison to facial skin [99].”
From Steroidogenesis in the skin: Implications for local immune functions (2013) · cites this paper as [99]
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- Authors
- Sakurai, N., Miki, Y., Suzuki, T., Watanabe, K., Narita, T., Ando, K., Yung, T. M. C., Aoki, D., Sasano, H., & Handa, H.
- Affiliations
- Tokyo Institute of Technology (JP)
- Tohoku University (JP)
- Keio University (JP)
- Corresponding author
- Handa, Hiroshi
- Venue
- The Journal of Steroid Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 99
- Issue
- 4-5
- Pages
- 174-181
- ISSN
- 0960-0760 (ISSN-L)
- OpenAlex
- W1980738399
- Cited by
- 60
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Tokyo Tech Research Repository (Tokyo Institute of Technology) (submittedVersion)
- Funders
- Ministry of Education
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- HSD17B12, tissue distribution, fatty acid synthesis, lipid metabolism, steroid metabolism, endocrine organs, liver, skeletal muscle, immunohistochemistry
- MeSH terms
- Breast Neoplasms, Female, Humans, Male, Organ Specificity, 17-Hydroxysteroid Dehydrogenases, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction, Cell Line, Tumor
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