Secreted protein acidic and rich in cysteine (SPARC) regulates thermogenesis in white and brown adipocytes
Abstract
SPARC, also known as osteonectin, is well known for its physiological roles in bone formation and tissue remodeling, as well as in cancer pathology; however, evidence regarding its function in adipocytes is lacking. The present study explored the physiological role of SPARC in cultured 3T3-L1 white and HIB1B brown adipocytes of murine cell lines. Treatment of recombinant SPARC upregulated the fat browning marker proteins and genes in white adipocytes and activated brown adipocytes. Conversely, knockdown of Sparc markedly reduced these genes and proteins in both cell lines. In addition, recombinant SPARC inhibited expression of adipogenic and lipogenic proteins but elevated lipolytic and fatty acid oxidation proteins. Furthermore, in silico analysis revealed that SPARC directly interacted and regulated VEGF in adipocytes. In conclusion, SPARC acts as a regulatory protein in both white and brown adipocytes by controlling thermogenesis and is thus regarded as a possible therapeutic target for treatment of obesity.
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SPARC inhibits the initial stage of adipogenesis by inhibiting mitotic clonal expansion of pre-adipocytes.
“Its function is related to the inhibition in the initial stage of adipogenesis through inhibiting the mitotic clonal expansion of pre-adipocytes [27].”
From Adipokines, Myokines, and Hepatokines: Crosstalk and Metabolic Repercussions (2021) · cites this paper as [27]
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Recinella, L., Orlando, G., Ferrante, C., Chiavaroli, A., Brunetti, L., & Leone, S. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.578966 · Cited by 261 · see citing works
Adipokines, Myokines, and Hepatokines: Crosstalk and Metabolic Repercussions
de Oliveira dos Santos, A. R., de Oliveira Zanuso, B., Miola, V. F. B., Barbalho, S. M., Santos Bueno, P. C., Flato, U. A. P., Detregiachi, C. R. P., Buchaim, D. V., Buchaim, R. L., Tofano, R. J., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22052639 · Cited by 189 · see citing works
- Authors
- Mukherjee, S., Choi, M. J., Kim, S. W., & Yun, J. W.
- Affiliations
- Daegu University (KR)
- Catholic Kwandong University (KR)
- Corresponding author
- Yun, Jong Won
- Venue
- Molecular and Cellular Endocrinology
- Publisher
- Elsevier BV
- Published
- 2020-04
- Volume
- 506
- Pages
- 110757
- ISSN
- 0303-7207 (ISSN-L)
- OpenAlex
- W3005899521
- Cited by
- 24
- References
- 63
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 6 · 2020: 4
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- SPARC, thermogenesis, brown adipocytes, white adipocytes, adipocyte browning, vascular endothelial growth factor, lipolysis, fatty acid oxidation, adipogenesis, lipogenesis, obesity, adipose tissue
- MeSH terms
- Animals, Cells, Cultured, Recombinant Proteins, Osteonectin, Thermogenesis, 3T3-L1 Cells, Lipogenesis, Adipogenesis, Lipid Metabolism, Mice, Adipocytes, Brown, Adipocytes, White, Cell Transdifferentiation
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