A multireceptor genetic approach uncovers an ordered integration of VNO sensory inputs in the accessory olfactory bulb
Open accessAbstract
Pheromone detection by the vomeronasal organ (VNO) is thought to rely on activation of specific receptors from the V1R and V2R gene families, but the central representation of pheromone receptor activation remains poorly understood. We generated transgenic mouse lines in which projections from multiple populations of VNO neurons, each expressing a distinct V1R, are differentially labeled with fluorescent proteins. This approach revealed that inputs from neurons expressing closely related V1Rs intermingle within shared, spatially conserved domains of the accessory olfactory bulb (AOB). Mitral cell-glomerular connectivity was examined by injecting intracellular dyes into AOB mitral cells and monitoring dendritic contacts with genetically labeled glomeruli. We show that individual mitral cells extend dendrites to glomeruli associated with different, but likely closely related, V1Rs. This organization differs from the labeled line of OR signaling in the main olfactory system and suggests that integration of information may already occur at the level of the AOB.
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Axons from VNO neurons expressing a given VR project to multiple glomeruli in the accessory olfactory bulb, where they form synaptic connections with AOB mitral/tufted cells, and AOB mitral cells form synapses within multiple glomeruli.
“In turn, axons from VNO neurons expressing a given VR project to multiple glomeruli in the accessory olfactory bulb (AOB) (Belluscio et al., 1999; Rodriguez et al., 1999), where they form synaptic connections with AOB mitral/tufted cells, and AOB mitral cells form synapses within multiple glomeruli (Wagner et al., 2006).”
From Neuronal Representation of Social Information in the Medial Amygdala of Awake Behaving Mice (2017) · cites this paper as Wagner et al., 2006
VNO neurons expressing a given VR project to multiple and spatially distributed glomeruli in the AOB, while AOB output neurons send dendrites to multiple glomeruli.
“VNO neurons expressing a given VR project to multiple and spatially distributed glomeruli in the AOB, while AOB output neurons send dendrites to multiple glomeruli (Belluscio et al., 1999; Dulac and Torello, 2003; Wagner et al., 2006).”
From Neuronal Representation of Social Information in the Medial Amygdala of Awake Behaving Mice (2017) · cites this paper as Wagner et al., 2006
The distributed non-topographic representation of each stimulus type mirrors the distributed organization in the AOB and suggests that information sorting may further be accomplished through selective projection to downstream regions.
“Such distributed non-topographic representation of each stimulus type mirrors the distributed organization in the AOB (Belluscio et al., 1999; Rodriguez et al., 1999; Wagner et al., 2006) and suggests that information sorting may further be accomplished through selective projection to downstream regions.”
From Neuronal Representation of Social Information in the Medial Amygdala of Awake Behaving Mice (2017) · cites this paper as Wagner et al., 2006
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- Authors
- Wagner, S., Gresser, A. L., Torello, A. T., & Dulac, C.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Wagner, Shlomo
- Dulac, Catherine
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 50
- Issue
- 5
- Pages
- 697-709
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2070841172
- Cited by
- 144
- References
- 45
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 10 · 2023: 7 · 2022: 1 · 2021: 10 · 2020: 12 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 8 · 2015: 12 · 2014: 9 · 2013: 13 · 2012: 6
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2603 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Deafness and Other Communication Disorders (R01DC003903)
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- vomeronasal organ, accessory olfactory bulb, pheromone detection, mitral cells, sensory integration
- MeSH terms
- Animals, Brain Mapping, Cell Line, Dendrites, Mice, Inbred C57BL, Mice, Transgenic, Neurons, Afferent, Olfactory Bulb, Olfactory Pathways, Smell, Protein Structure, Tertiary, Genes, Reporter, Vomeronasal Organ, Receptors, Pheromone, Mice
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