CoChat
Sign Up
Cited by 57Exercise and Physiological ResponsesCardiovascular and exercise physiologyNitric Oxide and Endothelin Effects

Positive correlation between plasma nitrite and performance during high-intensive exercise but not oxidative stress in healthy men

Dreißigacker, Ulrike, Wendt, Marcel, Wittke, Torge, Tsikas, Dimitrios, Maassen, Norbert·2010·Nitric Oxide
DOI 10.1016/j.niox.2010.05.003
Open this paper in CoChat — free account

Abstract

Several studies suggest that exercise is associated with elevated oxidative stress which diminishes NO bioavailability. The aim of the present study was to investigate a potential link between NO synthesis and bioavailability and oxidative stress in the circulation of subjects performing high-intensive endurance exercise. Twenty-two male healthy subjects cycled at 80% of their maximal workload. Cubital venous blood was taken before, during and after exercise, and heparinized plasma was generated. Plasma concentrations of nitrite and nitrate were quantified by GC-MS and of the oxidative stress biomarker 15(S)-8-iso-PGF(2alpha) by GC-MS/MS. pH and pCO(2) fell and HbO(2) increased upon exercise. The duration of the 80% phase (d80) was 740+/-210s. Subjects cycled at 89.2+/-3.3% of their peak oxygen uptake. Plasma concentration of nitrite (P<0.01) and 15(S)-8-iso-PGF(2alpha) (P<0.05) decreased significantly during exercise. At the end of exercise, plasma nitrite concentration correlated positively with d80 and performed work (w80) (each P<0.05). Changes in nitrate concentration also correlated positively with d80 (P<0.05) and w80/kg (P<0.01). These findings provide evidence of a favorable effect of nitrite on high-intensive endurance exercise. The lack of association between 15(S)-8-iso-PGF(2alpha) and NO bioavailability (nitrite concentration) and NO biosynthesis (nitrate concentration) suggest that oxidative stress, notably lipid peroxidation, is not linked to the l-arginine/NO pathway in healthy male subjects being on endurance exercise.

Integrity signals

Registry

Crossref · 10.1016/j.niox.2010.05.003

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Plasma nitrite concentration is positively associated with exercise capacity in humans.

    “Indeed, several studies have reported that plasma [nitrite] is positively associated with exercise capacity in humans [26–29].”

    From Dietary Nitrate Supplementation and Exercise Performance (2014) · cites this paper as [27]

  • Elevations in [NO3−] and [NO2−] following NO3− supplementation are associated with positive physiological effects that facilitate a greater capacity for muscular work and/or improved muscle contractile efficiency.

    “Elevations in [NO3−] and [NO2−] following NO3− supplementation have been observed in skeletal muscle [88,89,90,91] and plasma [53,64,92], and are associated with positive physiological effects [41,64,74,93] that facilitate a greater capacity for muscular work [51,53,59] and/or improved muscle contractile efficiency (i.e., a lower high-energy phosphate cost of force production) [51,94].”

    From Effects of Dietary Nitrate Supplementation on Weightlifting Exercise Performance in Healthy Adults: A Systematic Review (2020) · cites this paper as [93]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Dreißigacker, U., Wendt, M., Wittke, T., Tsikas, D., & Maassen, N.
Affiliations
Medizinische Hochschule Hannover (DE)
Corresponding author
Tsikas, Dimitrios
ORCID
Venue
Nitric Oxide
Publisher
Elsevier BV
Published
2010-09
Volume
23
Issue
2
Pages
128-135
ISSN
1089-8603 (ISSN-L)
DOI
10.1016/j.niox.2010.05.003
OpenAlex
W1966913042
Cited by
57
References
46
Citations by year
2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 9 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 10 · 2013: 5
FWCI
2.99
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Nitric Oxide (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
plasma nitrite, nitrate, nitric oxide bioavailability, nitric oxide production, oxidative stress, lipid peroxidation, exercise performance, L-arginine-NO pathway, work output
MeSH terms
Adult, Carbon Dioxide, Exercise Test, Feasibility Studies, Health, Hemoglobins, Humans, Hydrogen-Ion Concentration, Male, Gas Chromatography-Mass Spectrometry, Nitrates, Nitric Oxide, Nitrites, Oxygen, Physical Endurance, Reference Values, Time Factors, Lipid Peroxidation, Dinoprost, Biomarkers, Exercise, Oxidative Stress

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review