Real-time PCR quantification of precursor and mature microRNA
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TaqMan assays developed for miRNA detection and validation proved to be sensitive and easy to use.
“TaqMan assays developed for miRNA detection and validation proved to be sensitive and easy to use [42].”
From Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells (2014) · cites this paper as [42]
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Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
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“…each miRNA from the array-based profiling was quantified using the normalized threshold cycle number (DCt), in which DCt = [Ct (miRNA)] [Ct (U48)], and the relative expression level was calculated as 2 (DCt), which is commonly used in genome-wide miRNA profiling studies (Schmittgen et al., 2008).”
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- Authors
- Schmittgen, T. D., Lee, E. J., Jiang, J., Sarkar, A., Yang, L., Elton, T. S., & Chen, C.
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 44
- Issue
- 1
- Pages
- 31-38
- ISSN
- 1046-2023
- Cited by
- 535
- References
- 5
- Language
- en
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