Anatomical basis for cannabinoid-induced antinociception as revealed by intracerebral microinjections
Abstract
Cannabinoids suppress behavioral and neurophysiological responses to noxious stimuli in rodents when administered systemically. The purpose of this study was to extend previous studies of the site of cannabinoid analgesia. Rats were tested in the tail flick test before and after microinjections of the cannabinoid agonist WIN55, 212-2 (5 microg) into one of 17 different brain regions. WIN55,212-2 significantly elevated tail-flick latencies when injected into the amygdala, the lateral posterior and submedius regions of the thalamus, the superior colliculus and the noradrenergic A5 region. By contrast, pain behavior was unaffected by microinjections of the cannabinoid into the other 11 areas examined (prefrontal cortex, nucleus accumbens, lateral hypothalamus, substantia nigra, cuneiform nucleus, anterior pretectal, intralaminar, parafasicular, posterior, thalamic nuclei, as well as the ventral medial, ventral lateral nuclei in the posterior thalamus).
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Drug concentrations were based on reports in the literature and the authors' previous results.
“Drug concentrations were based on reports in the literature and our previous results (Martin et al, 1999;Ganon-Elazar and Akirav, 2009;Campolongo et al, 2009;Abush and Akirav, 2010;Segev and Akirav, 2011).”
From Cannabinoids prevent the development of behavioral and endocrine alterations in a rat model of intense stress (2012) · cites this paper as Martin et al, 1999
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- Authors
- Martin, W. J., Coffin, P. O., Attias, E., Balinsky, M., Tsou, K., & Walker, J. M.
- Affiliations
- Brown University (US)
- Corresponding author
- Walker, J.Michael
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1999-03
- Volume
- 822
- Issue
- 1-2
- Pages
- 237-242
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2009864778
- Cited by
- 164
- References
- 22
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 2 · 2020: 3 · 2019: 8 · 2018: 2 · 2017: 9 · 2016: 5 · 2015: 6 · 2014: 8 · 2013: 7 · 2012: 7
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health (5r01ns033247-08, 5k02da000375-09, 1f31da005617-01)
- National Institute on Drug Abuse (F31DA05617, K02 DA-00375)
- National Institute of Neurological Disorders and Stroke (NS 33247)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cognitive Neuroscience › Pain Management and Placebo Effect
- Keywords
- cannabinoid analgesia, antinociception, WIN 55,212-2, intracerebral microinjection, amygdala, thalamus, superior colliculus, tail-flick test, pain behavior, cannabinoid receptor agonists
- MeSH terms
- Amygdala, Analgesics, Animals, Brain Chemistry, Cannabinoids, Male, Microinjections, Morpholines, Naphthalenes, Nociceptors, Norepinephrine, Pain, Pain Measurement, Reaction Time, Superior Colliculi, Thalamus, Rats, Sprague-Dawley, Benzoxazines, Rats
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