Discrete carboxyl-terminal segments of apolipoprotein E mediate lipoprotein association and protein oligomerization
Open accessAbstract
The carboxyl terminus of apolipoprotein (apo) E is required for lipoprotein association and for tetramer formation. To correlate these roles with specific regions within the carboxyl terminus, a series of apoE3 variants with carboxyl-terminal truncations at residues 266, 244, 223, and 191 were expressed in Escherichia coli. As determined by gel permeation and sedimentation equilibrium centrifugation, the four truncated variants were monomeric in solution. Compared to native apoE3 (299 residues), all had reduced affinity for lipoproteins, as assessed by incubation of 125I-labeled proteins with plasma followed by fractionation of lipoprotein classes by gel filtration. The 266-residue variant associated with very low density lipoproteins and high density lipoproteins, but was partly non-lipoprotein-bound (25% of total). Shorter variants, with 244 or fewer residues, did not associate with very low density lipoproteins and only associated slightly (approximately 20%) with high density lipoproteins, with the major portion non-lipoprotein-bound (65-73%). After these proteins were injected into rabbits, the clearance rate was proportional to the plasma level of non-lipoprotein-bound protein. These results indicate lipoprotein association modulates the clearance of apoE, residues within the segment 267-299 are critical for apoE tetramerization and facilitate lipoprotein association, and residues within the segment 245-266 also contribute to lipoprotein association.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
APOE has two domains separated by a short hinge region.
“APOE has two domains separated by a short hinge region.”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Westerlund and Weisgraber, 1993
The N-terminal domain of APOE forms a soluble four-helix bundle and contains a patch of basic residues required for APOE binding to lipoprotein receptors.
“Its N-terminal domain forms a soluble four-helix bundle and contains a patch of basic residues required for APOE binding to lipoprotein receptors; the C-terminal domain is an amphipathic alpha-helix that mediates binding of APOE to the surface of lipoproteins (Westerlund and Weisgraber, 1993).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Westerlund and Weisgraber, 1993
The C-terminal domain of APOE is an amphipathic alpha-helix that mediates binding of APOE to the surface of lipoproteins.
“Its N-terminal domain forms a soluble four-helix bundle and contains a patch of basic residues required for APOE binding to lipoprotein receptors; the C-terminal domain is an amphipathic alpha-helix that mediates binding of APOE to the surface of lipoproteins (Westerlund and Weisgraber, 1993).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Westerlund and Weisgraber, 1993
The C-terminal domain of apoE contains amphipathic α-helices involved in binding to lipoproteins with high affinity.
“whereas the C-terminal domain contains amphipathic α-helices that are involved in binding to lipoproteins with high affinity (21, 22).”
From Role of the N- and C-Terminal Domains in Binding of Apolipoprotein E Isoforms to Heparan Sulfate and Dermatan Sulfate: A Surface Plasmon Resonance Study (2008) · cites this paper as [21]
ApoE self-associates through the C-terminal domain, and the C-terminal α-helical regions are responsible for the self-association.
“ApoE is known to self-associate through the C-terminal domain in solution and the C-terminal α-helical regions are responsible for the self-association (21, 45–47).”
From Role of the N- and C-Terminal Domains in Binding of Apolipoprotein E Isoforms to Heparan Sulfate and Dermatan Sulfate: A Surface Plasmon Resonance Study (2008) · cites this paper as [21]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Apolipoprotein E: Cholesterol Transport Protein with Expanding Role in Cell Biology
Mahley, R. W. (1988) · Science · doi:10.1126/science.3283935 · Cited by 4,028 · see citing works
Proteins, amino acids and peptides
Oppermann, R. H. (1943) · Journal of the Franklin Institute · doi:10.1016/s0016-0032(43)91372-9 · Cited by 2,171 · see citing works
Plasma lipoproteins: apolipoprotein structure and function.
Mahley, R. W., Innerarity, T. L., Rall, S. C., & Weisgraber, K. H. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34443-6 · Cited by 1,155 · see citing works
Self-citationSegrest, J., Jones, M., Loof, H. D., Brouillette, C. G., Venkatachalapathi, Y. V., & Anantharamaiah, G. M. (1992) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41536-6 · Cited by 868 · see citing works
Three-Dimensional Structure of the LDL Receptor-Binding Domain of Human Apolipoprotein E
Wilson, C., Wardell, M. R., Weisgraber, K. H., Mahley, R. W., & Agard, D. A. (1991) · Science · doi:10.1126/science.2063194 · Cited by 678 · see citing works
Self-citationAmphipathic helix motif: Classes and properties
Segrest, J. P., Loof, H. D., Dohlman, J. G., Brouillette, C. G., & Anantharamaiah, G. M. (1990) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.340080202 · Cited by 642 · see citing works
Weisgraber, K. H. (1990) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42621-5 · Cited by 304 · see citing works
Self-citationHuman apolipoprotein E3 in aqueous solution. I. Evidence for two structural domains.
Wetterau, J. R., Aggerbeck, L. P., Rall, S. C., & Weisgraber, K. H. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68778-2 · Cited by 293 · see citing works
Self-citationThe receptor-binding domain of human apolipoprotein E. Binding of apolipoprotein E fragments.
Innerarity, T. L., Friedlander, E. J., Rall, S. C., Weisgraber, K. H., & Mahley, R. W. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44180-9 · Cited by 276 · see citing works
Self-citationAggerbeck, L. P., Wetterau, J. R., Weisgraber, K. H., Wu, C. S., & Lindgren, F. T. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68779-4 · Cited by 229 · see citing works
Self-citationGianturco, S. H., Gotto, A. M., Hwang, S. L., Karlin, J. B., Lin, A., Prasad, S. C., & Bradley, W. A. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32655-3 · Cited by 162 · see citing works
[13] Isolation and characterization of apolipoprotein E
Rall, S. C., Weisgraber, K. H., & Mahley, R. W. (1986) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(86)28073-8 · Cited by 150 · see citing works
Self-citationThe Partial Specific Volumes, in Aqueous Solution, of Three Proteins
Dayhoff, M. O., Perlmann, G. E., & MacInnes, D. A. (1952) · Journal of the American Chemical Society · doi:10.1021/ja01130a018 · Cited by 143 · see citing works
Effect of lipid particle size on association of apolipoproteins with lipid.
Tajima, S., Yokoyama, S., & Yamamoto, A. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44607-2 · Cited by 134 · see citing works
Chylomicron-Chylomicron Remnant Clearance by Liver and Bone Marrow in Rabbits
Hussain, M. M., Mahley, R. W., Boyles, J. K., FAINARU, M., Brecht, W. J., & Lindquist, P. A. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)60570-8 · Cited by 124 · see citing works
Behavior of human apolipoprotein E in aqueous solutions and at interfaces.
Yokoyama, S., Kawai, Y., Tajima, S., & Yamamoto, A. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36247-6 · Cited by 114 · see citing works
Studies of Soybean Trypsin Inhibitor. I. Physicochemical Properties *
Wu, Y. V., & Scheraga, H. A. (1962) · Biochemistry · doi:10.1021/bi00910a025 · Cited by 111 · see citing works
Conformational and thermodynamic properties of apo A-1 of human plasma high density lipoproteins.
Tall, A. R., Shipley, G. G., & SMALL, D. M. (1976) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)33407-5 · Cited by 100 · see citing works
Horie, Y., Fazio, S., Westerlund, J. R., Weisgraber, K. H., & Rall, S. C. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)46040-1 · Cited by 94 · see citing works
Self-citationVogel, T., Weisgraber, K. H., Zeevi, M. I., Ben-Artzi, H., Levanon, A. Z., Rall, S. C., Innerarity, T. L., Hui, D. Y., Taylor, J. M., & Kanner, D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.24.8696 · Cited by 88 · see citing works
Self-citationMacromolecular characterization by sedimentation equilibrium in the preparative ultracentrifuge.
POLLET, R. J., Haase, B. A., & Standaert, M. L. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30265-x · Cited by 80 · see citing works
PHOSPHOLIPID BINDING STUDIES WITH SYNTHETIC APOLIPOPROTEIN FRAGMENTS *
Sparrow, J. T., & Gotto, A. M. (1980) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1980.tb21300.x · Cited by 79 · see citing works
Bothwell, M. A., Howlett, G. J., & Schachman, H. K. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38040-7 · Cited by 73 · see citing works
Structural properties and lipid binding of human apolipoprotein A-IV.
Weinberg, R. B., & Spector, M. S. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89159-1 · Cited by 73 · see citing works
The conformational properties of human plasma apolipoprotein C-II. A spectroscopic study.
Mantulin, W. W., Rohde, M. F., Gotto, A. M., & Pownall, H. J. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70628-0 · Cited by 64 · see citing works
The self-association of human apolipoprotein A-IV. Evidence for an in vivo circulating dimeric form.
Weinberg, R. B., & Spector, M. S. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38714-8 · Cited by 63 · see citing works
Reijngoud, D., & Phillips, M. C. (1982) · Biochemistry · doi:10.1021/bi00541a026 · Cited by 62 · see citing works
Lohse, P., Brewer, H., Meng, M., Skarlatos, S., LaRosa, J., & Brewer, H. (1992) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41380-x · Cited by 59 · see citing works
Conformational stability of the polypeptide components of human high density serum lipoprotein.
Reynolds, J. A. (1976) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)33052-1 · Cited by 49 · see citing works
Apolipoprotein E: phospholipid binding studies with synthetic peptides from the carboxyl terminus
Sparrow, J. T., Sparrow, D. A., Fernando, G. J. P., Culwell, A. R., Kovar, M., & Gotto, A. M. (1992) · Biochemistry · doi:10.1021/bi00119a015 · Cited by 38 · see citing works
Lipoproteins in pinnipeds: analysis of a high molecular weight form of apolipoprotein E.
Davis, R. A., Pierotti, V., Lauer, S., Hubl, S., McLean, J., Witztum, J., & Young, S. G. (1991) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41998-4 · Cited by 22 · see citing works
Belcher, J. D., Egan, J., Bridgman, G., Baker, R., & Flack, J. M. (1991) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42096-6 · Cited by 20 · see citing works
Yokoyama, S. (1990) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(90)90266-z · Cited by 18 · see citing works
Apolipoprotein variation among different species
Chan, L., & Li, W. (1991) · Current Opinion in Lipidology · doi:10.1097/00041433-199104000-00007 · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Apolipoprotein E: Structure-Function Relationships
Weisgraber, K. H. (1994) · Advances in Protein Chemistry · doi:10.1016/s0065-3233(08)60642-7 · Cited by 830 · see citing works
Self-citationStrittmatter, W. J., Saunders, A. M., Goedert, M., Weisgraber, K. H., Dong, L. M., Jakes, R., Huang, D. Y., Pericak-Vance, M., Schmechel, D., & Roses, A. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.11183 · Cited by 474 · see citing works
Self-citationApolipoprotein E: an important gene and protein to follow in laboratory medicine
Siest, G., Pillot, T., Régis-Bailly, A., Leininger-Muller, B., Steinmetz, J., Galteau, M. M., & Visvikis, S. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.8.1068 · Cited by 347 · see citing works
Apolipoprotein E isoforms and lipoprotein metabolism
Phillips, M. C. (2014) · IUBMB Life · doi:10.1002/iub.1314 · Cited by 328 · see citing works
Dong, L. M., Wilson, C., Wardell, M. R., Simmons, T., Mahley, R. W., Weisgraber, K. H., & Agard, D. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)31797-0 · Cited by 320 · see citing works
Self-citationHuman Apolipoprotein E4 Domain Interaction
Dong, L.-M., & Weisgraber, K. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.32.19053 · Cited by 318 · see citing works
Self-citationMorrow, J. A., Segall, M. L., Lund-Katz, S., Phillips, M. C., Knapp, M., Rupp, B., & Weisgraber, K. H. (2000) · Biochemistry · doi:10.1021/bi000099m · Cited by 315 · see citing works
Self-citationApolipoprotein E: From lipid transport to neurobiology
Hauser, P. S., Narayanaswami, V., & Ryan, R. O. (2011) · Progress in Lipid Research · doi:10.1016/j.plipres.2010.09.001 · Cited by 320 · see citing works
Pyrene: A Probe to Study Protein Conformation and Conformational Changes
Bains, G. K., Patel, A., & Narayanaswami, V. (2011) · Molecules · doi:10.3390/molecules16097909 · Cited by 287 · see citing works
High Density Lipoprotein Structure–Function and Role in Reverse Cholesterol Transport
Lund-Katz, S., & Phillips, M. C. (2010) · Subcellular Biochemistry · doi:10.1007/978-90-481-8622-8_7 · Cited by 262 · see citing works
Saito, H., Lund‐Katz, S., & Phillips, M. C. (2004) · Progress in Lipid Research · doi:10.1016/j.plipres.2004.05.002 · Cited by 216 · see citing works
Bains, G. K., Kim, S. H., Sorin, E. J., & Narayanaswami, V. (2012) · Biochemistry · doi:10.1021/bi3005285 · Cited by 201 · see citing works
Apolipoprotein-mediated Plasma Membrane Microsolubilization
Gillotte, K. L., Zaiou, M., Lund‐Katz, S., Anantharamaiah, G. M., Holvoet, P., Dhoest, A., Palgunachari, M. N., Segrest, J. P., Weisgraber, K. H., Rothblat, G. H., et al. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.4.2021 · Cited by 183 · see citing works
Self-citationRole of apolipoprotein E in neurodegenerative diseases
Giau, V. V., Bagyinszky, E., An, S. S. A., & Kim, S. (2015) · Neuropsychiatric Disease and Treatment · doi:10.2147/ndt.s84266 · Cited by 170 · see citing works
Hepatitis C Virus Attachment Mediated by Apolipoprotein E Binding to Cell Surface Heparan Sulfate
Jiang, J., Cun, W., Wu, X., Shi, Q., Tang, H., & Luo, G. G. (2012) · Journal of Virology · doi:10.1128/jvi.07222-11 · Cited by 170 · see citing works
Molecular basis of exchangeable apolipoprotein function
Narayanaswami, V., & Ryan, R. O. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(99)00176-6 · Cited by 152 · see citing works
Flowers, S. A., & Rebeck, G. W. (2020) · Neurobiology of Disease · doi:10.1016/j.nbd.2019.104724 · Cited by 149 · see citing works
Structural models of human apolipoprotein A-I
Brouillette, C. G., & Anantharamaiah, G. M. (1995) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(95)00018-8 · Cited by 146 · see citing works
Genetic heterogeneity of apolipoprotein E and its influence on plasma lipid and lipoprotein levels
de Knijff, P., van den Maagdenberg, A. M. J. M., Frants, R. R., & Havekes, L. M. (1994) · Human Mutation · doi:10.1002/humu.1380040303 · Cited by 136 · see citing works
Inter-molecular Coiled-coil Formation in Human Apolipoprotein E C-terminal Domain
Choy, N., Raussens, V., & Narayanaswami, V. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.09.059 · Cited by 131 · see citing works
Self-association of human apolipoprotein E3 and E4 in the presence and absence of phospholipid
Perugini, M. A., Schuck, P., & Howlett, G. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005565200 · Cited by 118 · see citing works
Lipid Binding-induced Conformational Change in Human Apolipoprotein E
Saito, H., Dhanasekaran, P., Baldwin, F., Weisgraber, K. H., Lund-Katz, S., & Phillips, M. C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106337200 · Cited by 113 · see citing works
Self-citationHuang, R. Y.-C., Garai, K., Frieden, C., & Gross, M. L. (2011) · Biochemistry · doi:10.1021/bi2010027 · Cited by 97 · see citing works
APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size
Windham, I. A., Powers, A. E., Ragusa, J. V., Wallace, E. D., Zanellati, M. C., Williams, V. H., Wagner, C. H., White, K. K., & Cohen, S. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202305003 · Cited by 96 · see citing works
Association of Rare <i>APOE</i> Missense Variants V236E and R251G With Risk of Alzheimer Disease
Le Guen, Y., Belloy, M. E., Grenier-Boley, B., de Rojas, I., Castillo-Morales, A., Jansen, I., Nicolas, A., Bellenguez, C., Dalmasso, C., Küçükali, F., et al. (2022) · JAMA Neurology · doi:10.1001/jamaneurol.2022.1166 · Cited by 93 · see citing works
Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
Morrow, J. A., Arnold, K. S., & Weisgraber, K. H. (1999) · Protein Expression and Purification · doi:10.1006/prep.1999.1069 · Cited by 94 · see citing works
Self-citationTwo-step Mechanism of Binding of Apolipoprotein E to Heparin
Futamura, M., Dhanasekaran, P., Handa, T., Phillips, M. C., Lund-Katz, S., & Saito, H. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m411719200 · Cited by 91 · see citing works
Characterization of the Heparin Binding Sites in Human Apolipoprotein E
Saito, H., Dhanasekaran, P., Nguyen, D., Baldwin, F., Weisgraber, K. H., Wehrli, S., Phillips, M. C., & Lund-Katz, S. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m213207200 · Cited by 90 · see citing works
Self-citationZhou, S., Huang, Y., Chen, Y., Liu, S., Xu, M., Jiang, T., Song, Q., Jiang, G., Gu, X. Q., Gao, X., et al. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.119795 · Cited by 89 · see citing works
Nguyen, D., Dhanasekaran, P., Nickel, M., Nakatani, R., Saito, H., Phillips, M. C., & Lund-Katz, S. (2010) · Biochemistry · doi:10.1021/bi1017655 · Cited by 89 · see citing works
Effects of Polymorphism on the Lipid Interaction of Human Apolipoprotein E
Saito, H., Dhanasekaran, P., Baldwin, F., Weisgraber, K. H., Phillips, M. C., & Lund-Katz, S. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304814200 · Cited by 89 · see citing works
Self-citationIsoform- and cell-state-specific lipidation of ApoE in astrocytes
Lindner, K., Beckenbauer, K., van Ek, L. C., Titeca, K., de Leeuw, S. M., Awwad, K., Hanke, F., Korepanova, A. V., Rybin, V., van der Kam, E. L., et al. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.110435 · Cited by 84 · see citing works
Acharya, P., Segall, M. L., Zaiou, M., Morrow, J., Weisgraber, K. H., Phillips, M. C., Lund-Katz, S., & Snow, J. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(02)00263-9 · Cited by 82 · see citing works
Self-citationNarayanaswami, V., Maiorano, J. N., Dhanasekaran, P., Ryan, R. O., Phillips, M. C., Lund‐Katz, S., & Davidson, W. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313318200 · Cited by 77 · see citing works
Structure−Function Studies of Human Apolipoprotein A-V: A Regulator of Plasma Lipid Homeostasis
Beckstead, J. A., Oda, M. N., Martin, D. D. O., Forte, T. M., Bielicki, J. K., Berger, T., Luty, R., Kay, C. M., & Ryan, R. O. (2003) · Biochemistry · doi:10.1021/bi034509t · Cited by 76 · see citing works
Gau, B., Garai, K., Frieden, C., & Gross, M. L. (2011) · Biochemistry · doi:10.1021/bi200911c · Cited by 74 · see citing works
Lee, Y., Kockx, M., Raftery, M. J., Jessup, W., Griffith, R., & Kritharides, L. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m900430-mcp200 · Cited by 74 · see citing works
Hubin, E., Verghese, P. B., van Nuland, N., & Broersen, K. (2019) · FEBS Letters · doi:10.1002/1873-3468.13428 · Cited by 73 · see citing works
The Association−Dissociation Behavior of the ApoE Proteins: Kinetic and Equilibrium Studies
Garai, K., & Frieden, C. (2010) · Biochemistry · doi:10.1021/bi101407m · Cited by 67 · see citing works
The emerging role of the first 17 amino acids of huntingtin in Huntington’s disease
Arndt, J. R., Chaibva, M., & Legleiter, J. A. (2015) · BioMolecular Concepts · doi:10.1515/bmc-2015-0001 · Cited by 69 · see citing works
Effect of Arginine 172 on the Binding of Apolipoprotein E to the Low Density Lipoprotein Receptor
Morrow, J. A., Arnold, K. S., Dong, J., Balestra, M. E., Innerarity, T. L., & Weisgraber, K. H. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.4.2576 · Cited by 67 · see citing works
Self-citationThe helix bundle: A reversible lipid binding motif
Narayanaswami, V., Kiss, R. S., & Weers, P. M. M. (2009) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2009.09.009 · Cited by 67 · see citing works
Molecular Mechanism of Apolipoprotein E Binding to Lipoprotein Particles
Nguyen, D., Dhanasekaran, P., Phillips, M. C., & Lund‐Katz, S. (2009) · Biochemistry · doi:10.1021/bi9000694 · Cited by 63 · see citing works
Domains of Apolipoprotein E Contributing to Triglyceride and Cholesterol Homeostasis in Vivo
Kypreos, K. E., van Dijk, K. W., van der Zee, A., Havekes, L. M., & Zannis, V. I. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100418200 · Cited by 62 · see citing works
Qiu, S., Korwek, K. M., & Weeber, E. J. (2005) · Neurobiology of Learning and Memory · doi:10.1016/j.nlm.2005.08.009 · Cited by 61 · see citing works
Lipid Association-induced N- and C-terminal Domain Reorganization in Human Apolipoprotein E3
Narayanaswami, V., Szeto, S. S. W., & Ryan, R. O. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102953200 · Cited by 53 · see citing works
Chetty, P. S., Mayne, L., Lund-Katz, S., Englander, S. W., & Phillips, M. C. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1617523114 · Cited by 58 · see citing works
Carboxyl-terminal Domain Truncation Alters Apolipoprotein A-I in Vivo Catabolism
Schmidt, H., Remaley, A. T., Stonik, J. A., Ronan, R., Wellmann, A., Fairwell, T., Zech, L. A., Brewer, H. B., & Hoeg, J. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.10.5469 · Cited by 58 · see citing works
Interaction with Amyloid β Peptide Compromises the Lipid Binding Function of Apolipoprotein E
Tamamizu‐Kato, S., Cohen, J. K., Drake, C. B., Kosaraju, M. G., Drury, J., & Narayanaswami, V. (2008) · Biochemistry · doi:10.1021/bi702097s · Cited by 55 · see citing works
Yamauchi, Y., Deguchi, N., Takagi, C., Tanaka, M., Dhanasekaran, P., Nakano, M., Handa, T., Phillips, M. C., Lund-Katz, S., & Saito, H. (2008) · Biochemistry · doi:10.1021/bi8003999 · Cited by 53 · see citing works
Sakamoto, T., Tanaka, M., Vedhachalam, C., Nickel, M., Nguyen, D., Dhanasekaran, P., Phillips, M. C., Lund-Katz, S., & Saito, H. (2008) · Biochemistry · doi:10.1021/bi701923h · Cited by 54 · see citing works
Flowers, S. A., Grant, O. C., Woods, R. J., & Rebeck, G. W. (2020) · Glycobiology · doi:10.1093/glycob/cwz084 · Cited by 52 · see citing works
Recombinant apolipoproteins for the treatment of vascular diseases
Sirtori, C. R., Calabresi, L., & Franceschini, G. (1999) · Atherosclerosis · doi:10.1016/s0021-9150(98)00247-0 · Cited by 51 · see citing works
Pauw, M. D., Vanloo, B., Weisgraber, K. H., & Rosseneu, M. (1995) · Biochemistry · doi:10.1021/bi00034a030 · Cited by 50 · see citing works
Self-citationReelin and apoE actions on signal transduction, synaptic function and memory formation
Rogers, J. T., & Weeber, E. J. (2008) · Neuron Glia Biology · doi:10.1017/s1740925x09990184 · Cited by 49 · see citing works
Li, X., Kypreos, K. E., Zanni, E. E., & Zannis, V. I. (2003) · Biochemistry · doi:10.1021/bi027093c · Cited by 49 · see citing works
Fisher, C. B., Wang, J., Francis, G. A., Sykes, B. D., Kay, C. M., & Ryan, R. O. (1997) · Biochemistry and Cell Biology · doi:10.1139/bcb-75-1-45 · Cited by 49 · see citing works
Modulation of the lipid binding properties of the N‐terminal domain of human apolipoprotein E3
Weers, P. M. M., Narayanaswami, V., & Ryan, R. O. (2001) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2001.02282.x · Cited by 47 · see citing works
Structural and functional variations in human apolipoprotein E3 and E4.
Chou, C.-Y., Jen, W.-P., Hsieh, Y.-H., Shiao, M.-S., & Chang, G.-G. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m511077200 · Cited by 46 · see citing works
Wang, G., Pierens, G. K., Treleaven, W. D., Sparrow, J. T., & Cushley, R. J. (1996) · Biochemistry · doi:10.1021/bi960934t · Cited by 45 · see citing works
Function and Comorbidities of Apolipoprotein E in Alzheimer′s Disease
Leduc, V., Domenger, D., Beaumont, L. D., Lalonde, D., Jasmin, S. B., & Poirier, J. (2011) · International Journal of Alzheimer s Disease · doi:10.4061/2011/974361 · Cited by 45 · see citing works
Brousseau, M., Santamarina-Fojo, S., Zech, L. A., Bérard, A. M., Vaisman, B. L., Meyn, S. M., Powell, D., Brewer, H. B., & Hoeg, J. M. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118614 · Cited by 44 · see citing works
Effect of Carboxyl-Terminal Truncation on Structure and Lipid Interaction of Human Apolipoprotein E4
Tanaka, M., Vedhachalam, C., Sakamoto, T., Dhanasekaran, P., Phillips, M. C., Lund-Katz, S., & Saito, H. (2006) · Biochemistry · doi:10.1021/bi060023b · Cited by 43 · see citing works
LilrB3 is a putative cell surface receptor of APOE4
Zhou, J., Wang, Y., Huang, G., Yang, M., Zhu, Y., Jin, C., Jing, D., Ji, K., & Shi, Y. (2023) · Cell Research · doi:10.1038/s41422-022-00759-y · Cited by 44 · see citing works
Patel, A., Khumsupan, P., & Narayanaswami, V. (2010) · Biochemistry · doi:10.1021/bi901902e · Cited by 43 · see citing works
Structural Analysis of Lipoprotein E Particles
Schneeweis, L. A., Koppaka, V., Lund-Katz, S., Phillips, M. C., & Axelsen, P. H. (2005) · Biochemistry · doi:10.1021/bi050872j · Cited by 39 · see citing works
Deroo, S., Stengel, F., Mohammadi, A., Henry, N., Hubin, E., Krammer, E., Aebersold, R. H., & Raussens, V. (2014) · ACS Chemical Biology · doi:10.1021/cb500994j · Cited by 40 · see citing works
ApoE associated with lipid has a reduced capacity to inhibit β-amyloid fibril formation
Beffert, U., & Poirier, J. (1998) · Neuroreport · doi:10.1097/00001756-199810050-00031 · Cited by 41 · see citing works
High‐affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid‐β
Ghosh, S., Sil, T. B., Dolai, S., & Garai, K. (2019) · FEBS Journal · doi:10.1111/febs.14988 · Cited by 40 · see citing works
Nikoulin, I. R., & Curtiss, L. K. (1998) · Journal of Clinical Investigation · doi:10.1172/jci1099 · Cited by 39 · see citing works
LaDu, M. J., Stine, Narita, M., Getz, G. S., Reardon, C. A., & Bu, G. (2006) · Biochemistry · doi:10.1021/bi051765s · Cited by 36 · see citing works
Examination of Lipid-bound Conformation of Apolipoprotein E4 by Pyrene Excimer Fluorescence
Drury, J., & Narayanaswami, V. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m414019200 · Cited by 37 · see citing works
Wang, H., Eschweiler, J. D., Cui, W., Zhang, H., Frieden, C., Ruotolo, B. T., & Gross, M. L. (2019) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-019-02148-z · Cited by 36 · see citing works
Orientation and mode of lipid-binding interaction of human apolipoprotein E C-terminal domain
Raussens, V., Drury, J., Forte, T. M., Choy, N., Goormaghtigh, E., Ruysschaert, J., & Narayanaswami, V. (2005) · Biochemical Journal · doi:10.1042/bj20041536 · Cited by 35 · see citing works
Lins, L., Thomas‐Soumarmon, A., Pillot, T., Vandekerckhove, J. S., Rosseneu, M., & Brasseur, R. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0730758.x · Cited by 36 · see citing works
Perugini, M. A., Schuck, P., & Howlett, G. J. (2002) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2002.03319.x · Cited by 34 · see citing works
The C Terminus of Apolipoprotein A-V Modulates Lipid-binding Activity
Beckstead, J. A., Mauldin, K., Gupta, V., Wan, C.-P. L., Cook, V. R., Weinberg, R. B., Weers, P. M. M., & Ryan, R. O. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m611797200 · Cited by 35 · see citing works
Pillot, T., Goethals, M., Vanloo, B., Lins, L., Brasseur, R., Vandekerckhove, J. S., & Rosseneu, M. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00650.x · Cited by 35 · see citing works
Weers, P. M. M., Narayanaswami, V., Choy, N., Luty, R., Hicks, L. D., Kay, C. M., & Ryan, R. O. (2002) · Biophysical Chemistry · doi:10.1016/s0301-4622(02)00300-9 · Cited by 33 · see citing works
Mishra, V., Palgunachari, M. N., Lund‐Katz, S., Phillips, M. C., Segrest, J. P., & Anantharamaiah, G. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.4.1602 · Cited by 33 · see citing works
Hoffer, M. J. V., Niththyananthan, S., Naoumova, R. P., Kibirige, M. S., Frants, R. R., Havekes, L. M., & Thompson, G. R. (1996) · Atherosclerosis · doi:10.1016/0021-9150(96)05819-4 · Cited by 32 · see citing works
Sheng, Z., Prorok, M., Brown, B., & Castellino, F. (2008) · Neuropharmacology · doi:10.1016/j.neuropharm.2008.05.016 · Cited by 32 · see citing works
The HepG2 Extracellular Matrix Contains Separate Heparinase- and Lipid-releasable Pools of ApoE
Burgess, J. W., Gould, D. R., & Marcel, Y. L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5645 · Cited by 30 · see citing works
Apo<scp>E</scp>: The role of conserved residues in defining function
Frieden, C. (2015) · Protein Science · doi:10.1002/pro.2597 · Cited by 29 · see citing works
Kypreos, K. E., Teusink, B., Dijk, K. O. W., Havekes, L. M., & Zannis, V. I. (2001) · The FASEB Journal · doi:10.1096/fj.00-0882fje · Cited by 29 · see citing works
Structural Basis for the Recognition and Cross-linking of Amyloid Fibrils by Human Apolipoprotein E
Gunzburg, M. J., Perugini, M. A., & Howlett, G. J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m706425200 · Cited by 28 · see citing works
Garai, K., Baban, B. A., & Frieden, C. (2011) · Biochemistry · doi:10.1021/bi2006702 · Cited by 26 · see citing works
Dong, L., Innerarity, T. L., Arnold, K. S., Newhouse, Y. M., & Weisgraber, K. H. (1998) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32541-4 · Cited by 26 · see citing works
Self-citationRole of Apolipoprotein E in Alzheimer’s Disease
Zannis, V. I., Zanni, E. E., Makrides, S. C., Kardassis, D., & Aleshkov, S. B. (1998) · Springer eBooks · doi:10.1007/978-1-4899-0133-0_13 · Cited by 26 · see citing works
Tran, T. N., Kim, S. H., Gallo, C., Amaya, M., Kyees, J., & Narayanaswami, V. (2012) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2012.10.007 · Cited by 25 · see citing works
NMR Structure and Dynamics of a Receptor-active Apolipoprotein E Peptide
Raussens, V., Slupsky, C. M., Ryan, R. O., & Sykes, B. D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204043200 · Cited by 26 · see citing works
Yamamura, T., Dong, L.-M., & Yamamoto, A. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)33364-2 · Cited by 25 · see citing works
Peelman, F., Goethals, M., Vanloo, B., Labeur, C., Brasseur, R., Vandekerckhove, J. S., & Rosseneu, M. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.t01-2-00708.x · Cited by 25 · see citing works
Muñoz, S. S., Li, H., Ruberu, K. R., Chu, Q., Saghatelian, A., Ooi, L., & Garner, B. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra117.001278 · Cited by 24 · see citing works
Structure-guided Protein Engineering Modulates Helix Bundle Exchangeable Apolipoprotein Properties
Kiss, R. S., Weers, P. M. M., Narayanaswami, V., Cohen, J. K., Kay, C. M., & Ryan, R. O. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m302676200 · Cited by 24 · see citing works
ApoE: In Vitro Studies of a Small Molecule Effector
Mondal, T., Wang, H., DeKoster, G. T., Baban, B. A., Gross, M. L., & Frieden, C. (2016) · Biochemistry · doi:10.1021/acs.biochem.6b00324 · Cited by 23 · see citing works
Extracellular Interactions between Hepatitis C Virus and Secreted Apolipoprotein E
Li, Z., Li, Y., Bi, Y., Zhang, H., Yao, Y., Li, Q., Cun, W., & Dong, S. (2017) · Journal of Virology · doi:10.1128/jvi.02227-16 · Cited by 23 · see citing works
Hsieh, Y.-H., & Chou, C. (2011) · Journal of Biomedical Science · doi:10.1186/1423-0127-18-4 · Cited by 23 · see citing works
- Authors
- Westerlund, J. A., & Weisgraber, K. H.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-07
- Volume
- 268
- Issue
- 21
- Pages
- 15745-15750
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1659662945
- Cited by
- 164
- References
- 35
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 6 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 4
- FWCI
- 4.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL-41633, P01HL041633)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- apolipoprotein E, carboxyl-terminal truncation, protein oligomerization, tetramer formation, VLDL, high-density lipoprotein, plasma clearance, protein-lipid interactions
- MeSH terms
- Animals, Apolipoproteins E, Humans, Iodine Radioisotopes, Lipoproteins, HDL, Lipoproteins, VLDL, Male, Metabolic Clearance Rate, Mutation, Peptide Fragments, Polymers, Protein Binding, Rabbits
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
