The use of behavioral test batteries: Effects of training history
Abstract
Our laboratory uses a specific test battery for the initial assessment of phenotypic behavioral differences of transgenic, knockout, and inbred strains of mice. Our standard battery includes: open field activity, light-dark exploration, rotarod, prepulse inhibition (PPI), acoustic startle habituation, conditioned fear, Morris water maze, and hot plate. Tests are run in the order listed, from least invasive to most invasive, to decrease the chance that behavioral responses are altered by prior test history. The studies presented here were designed around two questions. The first study asks if differences exist between mice that have undergone testing on different tasks and mice that are naïve to the test experience. The second study asks if the test order affects how an animal performs on subsequent tests. In the first experiment, C57BL/6J male mice were evaluated on all of the tests described above. The behavior of these 'test battery' mice was compared to aged matched naïve mice that were only tested on one test from the battery. Results indicate that on some tests, the behavior of 'test battery' mice was significantly different from the behavior of naïve mice, while on other tests there were no differences. For example, test battery mice responded differently in the open-field, rotarod, and hot-plate test, but behaved similar on the PPI and conditioned fear. Experiments in the second study were performed on male 129/SvEvTac (129S6) and C57BL/6J male mice. An abbreviated bat…
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The tests in the battery were administered from least to most invasive to minimize affecting behavior by prior test history.
“The tests in the battery were administered in the above listed order, from least to most invasive [61], to minimize affecting behavior by prior test history.”
From Normal cognition in transgenic BRI2-Aβ mice (2013) · cites this paper as [61]
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- Authors
- McIlwain, K. L., Merriweather, M. Y., Yuva-Paylor, L. A., & Paylor, R.
- Affiliations
- Baylor College of Medicine (US)
- Corresponding author
- Paylor, Richard
- Venue
- Physiology & Behavior
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 73
- Issue
- 5
- Pages
- 705-717
- ISSN
- 0031-9384 (ISSN-L)
- OpenAlex
- W2088620464
- Cited by
- 579
- References
- 1
- Citations by year
- 2026: 13 · 2025: 21 · 2024: 20 · 2023: 22 · 2022: 39 · 2021: 18 · 2020: 26 · 2019: 28 · 2018: 22 · 2017: 20 · 2016: 27 · 2015: 32 · 2014: 27 · 2013: 25 · 2012: 22
- FWCI
- 27.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Physiology & Behavior (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG1832)
- Topics
- Health Sciences › Veterinary › Small Animals › Animal Behavior and Welfare Studies
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- test order effects, transgenic mice, knockout mice, inbred mouse strains, prepulse inhibition, fear conditioning, rotarod test, open field behavior, acoustic startle response, Morris water maze, hot plate, C57BL/6J mice, behavioral assessment
- MeSH terms
- Animals, Arousal, Behavior, Animal, Genetics, Behavioral, Male, Mice, Inbred C57BL, Mice, Inbred Strains, Mice, Transgenic, Phenotype, Species Specificity, Mice, Knockout, Mice
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