Improved haemocompatibility of cysteine-modified polymers via endogenous nitric oxide
Abstract
A novel method for improving the haemocompatibility of biomedical materials through endogenous nitric oxide (NO) is presented. L-cysteine was covalently immobilized onto two biomedical polymers: polyurethane (PU) and polyethylene terephthalate (PET). The L-cysteine content on the polymers was approximately 5-8 nmol/cm2 as quantified via a chemiluminescence-based assay. The haemocompatibility of the modified polymers was evaluated in terms of the number of adhered platelets when exposed to a platelet suspension labeled with Cr51. Platelet adherence on the L-cysteine-modified polymers was reduced more than 50% as compared to the control (glycine-modified polymers) when the platelet suspension contained plasma constituents. No difference in platelet adhesion was observed in the absence of plasma constituents. Further experiments demonstrated that NO was easily transferred to the L-cysteine-modified polymers from S-nitroso-albumin in PBS buffer. The NO was then released from the polymer. NO transfer or release was not observed for the control. The results suggest that L-cysteine-modified polymers are effective in reducing platelet adhesion via the transfer of NO from endogenous S-nitrosoproteins in plasma to the polymer followed by the subsequent release of NO. Thus, exploiting endogenous NO is a viable option for improving the haemocompatibility of biomaterials.
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Transnitrosation transfers the NO+ group from an RSNO to another free thiol, giving a circulating unlimited supply of NO in vivo.
“The transnitrosation reaction transfers the NO+ functional group from an RSNO species to another existing free thiol, thus achieving a circulating unlimited supply of NO in vivo.160”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [160]
Catalysis-based NO generating polymers generate NO from endogenous RSNOs or infused RSNO solutions through thiol transnitrosations and subsequent catalytic reactions using immobilized copper or selenium species.
“Instead of incorporating NO donors or NO functionalities into polymers, many efforts have been devoted to generate NO from endogenous RNSOs or infusion of RSNO solutions through thiol transnitrosations,160,253 and subsequent catalytic reactions using immobilized copper or selenium-based species”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [160]
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Self-citation
- Authors
- Duan, X., & Lewis, R. S.
- Affiliations
- Oklahoma State University (US)
- Corresponding author
- Lewis, Randy S.
- ORCID
- Lewis, Randy S.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2002-02
- Volume
- 23
- Issue
- 4
- Pages
- 1197-1203
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1990268295
- Cited by
- 75
- References
- 31
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 7 · 2015: 2 · 2014: 2 · 2013: 5 · 2012: 8
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- hemocompatibility, nitric oxide, cysteine, covalent immobilization, polyurethane, polyethylene terephthalate, platelet adhesion, nitric oxide release
- MeSH terms
- Animals, Biocompatible Materials, Blood, Cattle, Cysteine, Humans, Materials Testing, Nitric Oxide, Platelet Adhesiveness, Polyethylene Terephthalates, Polymers, Polyurethanes, Serum Albumin, Bovine, In Vitro Techniques
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