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Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis
Open accessAbstract
After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs for ecological studies it seems necessary to summarize reported pitfalls of this approach which will most likely lead to an erroneous description on the microbial diversity of a given habitat. The following article will cover specific aspects of sample collection, cell lysis, nucleic acid extraction, PCR amplification, separation of amplified DNA, application of nucleic probes and data analysis.
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Metadata
- Authors
- v. Wintzingerode, F.
- Venue
- FEMS Microbiology Reviews
- Publisher
- Wiley
- Published
- 1997-11
- Volume
- 21
- Issue
- 3
- Pages
- 213-229
- ISSN
- 0168-6445 (ISSN-L)
- OpenAlex
- W2125862549
- Cited by
- 2,063
- References
- 1
- FWCI
- 44.42
- Type
- journal article
- Language
- en
- Open access
- bronze
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