Effects of probiotic administration upon the composition and enzymatic activity of human fecal microbiota in patients with irritable bowel syndrome or functional diarrhea
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In a clinical trial, 10 patients suffering from irritable bowel syndrome or functional diarrhea were administered the probiotic preparation VSL-3. Preliminary results indicated that administration of VSL-3 improved the clinical picture and changed the composition and biochemistry of fecal microbiota. Titer variations of intestinal bacterial groups were evaluated by culture and PCR techniques. A significant increase in lactobacilli, bifidobacteria and Streptococcus thermophilus was observed as a consequence of probiotic treatment, while enterococci, coliforms, Bacteroides and Clostridium perfringens did not change significantly. The strains Bifidobacterium infantis Y1 and Bifidobacterium breve Y8, included in VSL-3, were specifically detected in feces of patients treated with the probiotic by using strain-specific PCR primers. In addition, fecal beta-galactosidase increased and urease activities decreased as a result of changes in the intestinal microbiota induced by VSL-3 administration.
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Probiotics might reshape the intestinal ecosystem, generating an ecological milieu unfavorable for the growth of harmful species by increasing the number of Lactobacilli and Bifidobacteria that stabilize the intestinal microbiota.
“However, it has been suggested that probiotics might reshape the intestinal ecosystem generating an ecological milieu that is unfavorable for the growth of harmful species by increasing the number of Lactobacilli and Bifidobacteria [199] that will stabilize the intestinal microbiota [174,179] .”
From Gut microbiota role in irritable bowel syndrome: New therapeutic strategies (2016) · cites this paper as [199]
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- Authors
- Brigidi, P., Vitali, B., Swennen, E., Bazzocchi, G., & Matteuzzi, D.
- Affiliations
- University of Bologna (IT)
- Corresponding author
- Brigidi, Patrizia
- Venue
- Research in Microbiology
- Publisher
- Elsevier BV
- Published
- 2001-10
- Volume
- 152
- Issue
- 8
- Pages
- 735-741
- ISSN
- 0769-2609 (ISSN-L)
- 0923-2508
- OpenAlex
- W2089275594
- Cited by
- 188
- References
- 27
- Citations by year
- 2024: 1 · 2023: 3 · 2021: 7 · 2020: 7 · 2019: 3 · 2018: 3 · 2017: 7 · 2016: 8 · 2015: 6 · 2014: 8 · 2013: 10 · 2012: 7
- FWCI
- 14.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Research in Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- probiotics, VSL#3, irritable bowel syndrome, functional diarrhea, fecal microbiota, lactobacilli, Bifidobacterium, Bifidobacterium infantis, Bifidobacterium breve, Streptococcus thermophilus, beta-galactosidase, urease activity
- MeSH terms
- Adult, Bacteria, Bifidobacterium, Colonic Diseases, Functional, Diarrhea, Feces, Female, Humans, Male, Middle Aged, Streptococcus, Polymerase Chain Reaction, Probiotics
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