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The β-Globin LCR Is Not Necessary for an Open Chromatin Structure or Developmentally Regulated Transcription of the Native Mouse β-Globin Locus
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The murine beta-globin locus control region (LCR) was deleted from its native chromosomal location. The approximately 25 kb deletion eliminates all sequences and structures homologous to those defined as the human LCR. In differentiated ES cells and erythroleukemia cells containing the LCR-deleted chromosome, DNasel sensitivity of the beta-globin domain is established and maintained, developmental regulation of the locus is intact, and beta-like globin RNA levels are reduced 5%-25% of normal. Thus, in the native murine beta-globin locus, the LCR is necessary for normal levels of transcription, but other elements are sufficient to establish the open chromatin structure, transcription, and developmental specificity of the locus. These findings suggest a contributory rather than dominant function for the LCR in its native location.
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“previous studies deleting the entire β-globin SE showed that the β-globin genes remain accessible and marked by histone acetylation;55–57”
From Super-enhancers include classical enhancers and facilitators to fully activate gene expression (2023) · cites this paper as [56]
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Cites
Position-independent, high-level expression of the human β-globin gene in transgenic mice
Grosveld, F. G., van Assendelft, G. B., Greaves, D. R., & Kollias, G. A. (1987) · Cell · doi:10.1016/0092-8674(87)90584-8 · Cited by 1,908
Cyclin D1 provides a link between development and oncogenesis in the retina and breast
Sicinski, P., Donaher, J. L., Parker, S. B., Li, T., Fazeli, A., Gardner, H., Haslam, S. Z., Bronson, R. T., Elledge, S. J., & Weinberg, R. A. (1995) · Cell · doi:10.1016/0092-8674(95)90034-9 · Cited by 1,021
Gu, H., Zou, Y.-R., & Rajewsky, K. (1993) · Cell · doi:10.1016/0092-8674(93)90644-6 · Cited by 989
Hematopoietic Commitment During Embryonic Stem Cell Differentiation in Culture
Keller, G. M., Kennedy, M. A., Papayannopoulou, T., & Wiles, M. V. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.473 · Cited by 874
Self-citationMoreau, P., Hen, R., Wasylyk, B., Everett, R., Gaub, M. P., & Chambon, P. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.22.6047 · Cited by 761
Multilineage gene expression precedes commitment in the hemopoietic system.Preprint
Hu, M., Krause, D. S., Greaves, M. F., Sharkis, S. J., Dexter, M., Heyworth, C. M., & Enver, T. (1997) · Genes & Development · doi:10.1101/gad.11.6.774 · Cited by 719
Self-citationMice lacking cyclin D1 are small and show defects in eye and mammary gland development.
Fantl, V, Stamp, G., Andrews, A., Rosewell, I., & Dickson, C. (1995) · Genes & Development · doi:10.1101/gad.9.19.2364 · Cited by 691
The "beta-like-globin" gene domain in human erythroid cells.
Tuan, D., Solomon, W., Li, Q., & London, I. M. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.19.6384 · Cited by 601
A common precursor for primitive erythropoiesis and definitive haematopoiesis
Kennedy, M., Firpo, M. T., Choi, K., Wall, C., Robertson, S. M., Kabrun, N., & Keller, G. M. (1997) · Nature · doi:10.1038/386488a0 · Cited by 585
Self-citationForrester, W. C., Epner, E. M., Driscoll, M. C., Enver, T., Brice, M. C., Papayannopoulou, T., & Groudine, M. T. (1990) · Genes & Development · doi:10.1101/gad.4.10.1637 · Cited by 464
Self-citationForrester, W. C., Takegawa, S., Papayannopoulou, T., Stamatoyannopoulos, G., & Groudine, M. T. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.24.10159 · Cited by 418
Self-citationProduction of Homozygous Mutant ES Cells with a Single Targeting Construct
Mortensen, R. M., Conner, D. A., Chao, S., Geisterfer-Lowrance, A. A. T., & Seidman, J. G. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.5.2391 · Cited by 415
Erythroid‐specific expression of human beta‐globin genes in transgenic mice.
Townes, T. M., Lingrel, J. B., Chen, H. Y., Brinster, R. L., & Palmiter, R. D. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb03841.x · Cited by 414
A developmentally stable chromatin structure in the human beta-globin gene cluster.
Forrester, W. C., Thompson, C. B., Elder, J. T., & Groudine, M. T. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.5.1359 · Cited by 376
Self-citationFiering, S., Epner, E. M., Robinson, K. M., Zhuang, Y., Telling, A., Hu, M., Martin, D. I. K., Enver, T., Ley, T. J., & Groudine, M. T. (1995) · Genes & Development · doi:10.1101/gad.9.18.2203 · Cited by 334
Self-citationTalbot, D., Collis, P., Antoniou, M. N., Vidal, M., Grosveld, F. G., & Greaves, D. R. (1989) · Nature · doi:10.1038/338352a0 · Cited by 330
Heterochromatin Effects on the Frequency and Duration of LCR-Mediated Gene Transcription
Milot, E., Strouboulis, J., Trimborn, T., Wijgerde, M., de Boer, E., Langeveld, A., Tan-Un, K., Vergeer, W., Yannoutsos, N., Grosveld, F., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81327-6 · Cited by 314
Enhancers increase the probability but not the level of gene expression.
Walters, M. C., Fiering, S., Eidemiller, J., Magis, W., Groudine, M. T., & Martin, D. I. K. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.15.7125 · Cited by 286
Self-citationβ-Globin gene inactivation by DNA translocation in γβ-thalassaemi
Kioussis, D., Vanin, E., deLange, T., Flavell, R. A., & Grosveld, F. G. (1983) · Nature · doi:10.1038/306662a0 · Cited by 264
Ryan, T. M., Behringer, R. R., Martin, N. C., Townes, T. M., Palmiter, R. D., & Brinster, R. L. (1989) · Genes & Development · doi:10.1101/gad.3.3.314 · Cited by 259
Madisen, L., & Groudine, M. T. (1994) · Genes & Development · doi:10.1101/gad.8.18.2212 · Cited by 258
Self-citationDriscoll, M. C., Dobkin, C. S., & Alter, B. P. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.19.7470 · Cited by 233
Hardison, R., Slightom, J. L., Gumucio, D. L., Goodman, M., Stojanovic, N., & Miller, W. (1997) · Gene · doi:10.1016/s0378-1119(97)00474-5 · Cited by 230
The role of insulator elements in defining domains of gene expression
Geyer, P. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80134-7 · Cited by 217
Forrester, W. C., van Genderen, C., Jenuwein, T., & Grosschedl, R. (1994) · Science · doi:10.1126/science.8066460 · Cited by 216
Developmental regulation of a cloned adult β-globin gene in transgenic mice
Magram, J., Chada, K. K., & Costantini, F. D. (1985) · Nature · doi:10.1038/315338a0 · Cited by 213
Unexpected relationships between four large deletions in the human β-globin gene cluster
Vanin, E. F., Henthom, P. S., Kioussis, D., Grosveld, F. G., & Smithies, O. (1983) · Cell · doi:10.1016/0092-8674(83)90103-4 · Cited by 210
Molecular analysis of the human beta-globin locus activation region.
Forrester, W. C., Novak, U., Gelinas, R. E., & Groudine, M. T. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.14.5439 · Cited by 209
Self-citationChen, J., Young, F. M., Bottaro, A., Stewart, V., Smith, R. K., & Alt, F. W. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06152.x · Cited by 188
Transcriptional enhancers act in cis to suppress position-effect variegation.Preprint
Walters, M. C., Magis, W., Fiering, S., Eidemiller, J., Scalzo, D., Groudine, M. T., & Martin, D. I. K. (1996) · Genes & Development · doi:10.1101/gad.10.2.185 · Cited by 185
Self-citationRepresentative in Vitro cDNA Amplification From Individual Hemopoietic Cells and Colonies
Brady, G., Barbara, M., & Iscove, N. N. (1990) · Cited by 179
Control of Globin Gene Transcription
Evans, T., Felsenfeld, G., & Reitman, M. L. (1990) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.06.110190.000523 · Cited by 169
Hug, B. A., Wesselschmidt, R. L., Fiering, S., Bender, M. A., Epner, E. M., Groudine, M. T., & Ley, T. J. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.6.2906 · Cited by 168
Self-citationReik, A., Telling, A., Zitnik, G., Cimbora, D., Epner, E., & Groudine, M. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.10.5992 · Cited by 163
Self-citationExpression of the human calcitonin/CGRP gene in lung and thyroid carcinoma.
Edbrooke, M. R., Parker, D., McVey, J. H., Riley, J. W., Sorenson, G. D., Pettengill, O. S., & CRAIG, R. K. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb03688.x · Cited by 148
Locus control regions: overcoming heterochromatin-induced gene inactivation in mammals
Kioussis, D., & Festenstein, R. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80008-1 · Cited by 144
Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNA
Treisman, R., & Maniatis, T. (1985) · Nature · doi:10.1038/315072a0 · Cited by 141
Regulation of β-globin gene expression: straightening out the locus
Martin, D. I. K., Fiering, S., & Groudine, M. T. (1996) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(96)80072-4 · Cited by 141
Self-citationRegulation of Globin Gene Expression in Erythroid Cells
Orkin, S. H. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.tb20697.x · Cited by 135
Formation of stable transcription complexes as assayed by analysis of individual templates.
Weintraub, H. M. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.16.5819 · Cited by 112
Kim, C. G., Epner, E. M., Forrester, W. C., & Groudine, M. T. (1992) · Genes & Development · doi:10.1101/gad.6.6.928 · Cited by 109
Self-citationMoon, A., & Ley, T. J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.19.7693 · Cited by 108
Fiering, S., Kim, C. G., Epner, E. M., & Groudine, M. T. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.18.8469 · Cited by 100
Self-citationDevelopmental regulation of human β-globin gene transcription: a switch of loyalties?
Engel, J. D. (1993) · Trends in Genetics · doi:10.1016/0168-9525(93)90248-g · Cited by 99
γδβ-Thalassaemias 1 and 2 are the result of a 100 kbp deletion in the human β-globin cluster
Taramelli, R., Kioussis, D., Vanin, E. F., Bartram, K., Groffen, J., Hurst, J., & Grosveld, F. G. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.17.7017 · Cited by 91
High-level erythroid expression of human alpha-globin genes in transgenic mice.
Ryan, T. M., Behringer, R. R., Townes, T. M., Palmiter, R. D., & Brinster, R. L. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.1.37 · Cited by 90
Dieken, E. S., Epner, E. M., Fiering, S., Fournier, R. E. K., & Groudine, M. T. (1996) · Nature Genetics · doi:10.1038/ng0296-174 · Cited by 89
Self-citationAsynchronous DNA replication within the human beta-globin gene locus.
Epner, E. M., Forrester, W. C., & Groudine, M. T. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.21.8081 · Cited by 88
Self-citationSimian virus 40 enhancer increases RNA polymerase density within the linked gene
Weber, F., & Schaffner, W. (1985) · Nature · doi:10.1038/315075a0 · Cited by 80
Bender, M. A., Reik, A., Close, J., Telling, A., Epner, E. M., Fiering, S., Hardison, R. C., & Groudine, M. T. (1998) · Blood · doi:10.1182/blood.v92.11.4394.423k48_4394_4403 · Cited by 68
Self-citationZhuang, Y., Kim, C. G., Bartelmez, S. H., Cheng, P.-F., Groudine, M. T., & Weintraub, H. M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.24.12132 · Cited by 59
Self-citationStarck, J., Sarkar, R., Romana, M., Bhargava, A., Scarpa, A. L., Tanaka, M., Chamberlain, J., Weissman, S., & Forget, B. G. (1994) · Blood · doi:10.1182/blood.v84.5.1656.1656 · Cited by 53
Bernet, A., Sabatier, S., Picketts, D., Ouazana, R., Morlé, F., Higgs, D. R., & Godet, J. (1995) · Blood · doi:10.1182/blood.v86.3.1202.1202 · Cited by 50
Grosschedl, R., & Marx, M. (1988) · Cell · doi:10.1016/0092-8674(88)90223-1 · Cited by 47
Developmental Programs of Human Erythroleukemia Cells: Globin Gene Expression and Methylation
Enver, T., Zhang, J. W., Anagnou, N., Stamatoyannopoulos, G., & PAPAYANNOPOULOU, T. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.11.4917 · Cited by 27
Self-citationThe Dynamics of Globin Gene Expression and Gene Therapy Vectors
Grosveld, F. G., de Boer, E., Dillon, N., Gribnau, J. H., Milot, É., Trimborn, T., Wijgerde, M., & FRASERA, P. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb10458.x · Cited by 24
The dynamics of globin gene expression and gene therapy vectors.
Grosveld, F. G., de Boer, E., Dillon, N., Fraser, P., Gribnau, J. H., Milot, É., Trimborn, T., & Wijgerde, M. (1998) · PubMed · Cited by 18
K562 human leukemia cell passages differ in embryonic globin gene expression
Rowley, P. T., Ohlsson‐Wilhelm, B. M., Wisniewski, L. P., Lozzio, C. B., & Lozzio, B. B. (1984) · Leukemia Research · doi:10.1016/0145-2126(84)90030-4 · Cited by 18
Rees, M. I., Worwood, M., THOMPSON, P. W., Gilbertson, C., & May, A. M. (1994) · British Journal of Haematology · doi:10.1111/j.1365-2141.1994.tb04927.x · Cited by 13
Preparation and properties of microcell hybrids.
Langston, A. A., & Fournier, R. E. K. (1993) · Cited by 7
Research works printed but not linked: 2
- Enver, 1988, Developmental programs of human erythroleukemia cells, Mol. Cell. Biol, 8, 4917
- Madisen, 1998, The immunoglobulin heavy chain LCR increases histone acetylation along linked c-myc genes, Mol. Cell. Biol, in press
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Self-citationLooping versus linking: toward a model for long-distance gene activationPreprint
Bulger, M., & Groudine, M. T. (1999) · Genes & Development · doi:10.1101/gad.13.19.2465 · Cited by 483
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Prasanth, K. V., & Spector, D. L. (2007) · Genes & Development · doi:10.1101/gad.1484207 · Cited by 408
Zappone, M., Galli, R., Catena, R., Meani, N., Biasi, S. D., Mattei, E., Tiveron, C., Vescovi, A. L., Lovell-Badge, R. H., Ottolenghi, S., et al. (2000) · Development · doi:10.1242/dev.127.11.2367 · Cited by 349
Metadata
- Authors
- Epner, E., Reik, A., Cimbora, D., Telling, A., Bender, M. A., Fiering, S., Enver, T., Martin, D. I. K., Kennedy, M., Keller, G., & Groudine, M.
- Affiliations
- Fred Hutch Cancer Center (US)
- National Jewish Health (US)
- University of Washington (US)
- Corresponding author
- Groudine, Mark
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 1998-10
- Volume
- 2
- Issue
- 4
- Pages
- 447-455
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2070298061
- Cited by
- 183
- References
- 60
- Citations by year
- 2025: 2 · 2023: 2 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 1
- FWCI
- 8.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- National Institutes of Health (5p30hd028834-10)
- National Heart, Lung, and Blood Institute (HL 48834)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK52854)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P30 HD28834)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Keywords
- beta-globin locus control region, beta-globin gene expression, DNase I sensitivity, embryonic stem cells, erythroleukemia cells, chromatin accessibility
- MeSH terms
- Animals, Chromatin, Deoxyribonucleases, Leukemia, Erythroblastic, Acute, Globins, Humans, Phenotype, Protein Conformation, Tumor Cells, Cultured, Transcriptional Activation, Gene Expression, Gene Deletion, Protein Structure, Tertiary, Mice
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