Talking about a Revolution

The sequence of the human genome

Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909

Initial sequencing and comparative analysis of the mouse genome

Mouse Genome Sequencing Consortium (2002) · Nature · doi:10.1038/nature01262 · Cited by 7,187

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943

A gene expression atlas of the central nervous system based on bacterial artificial chromosomes

Gong, S., Zheng, C., Doughty, M. L., Losos, K., Didkovsky, N., Schambra, U. B., Nowak, N. J., Joyner, A., Leblanc, G., Hatten, M. E., et al. (2003) · Nature · doi:10.1038/nature02033 · Cited by 2,108

Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting

Gu, H., Zou, Y.-R., & Rajewsky, K. (1993) · Cell · doi:10.1016/0092-8674(93)90644-6 · Cited by 989

Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development

Thomas, K. R., & Capecchi, M. R. (1990) · Nature · doi:10.1038/346847a0 · Cited by 896

Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase.

Metzger, D., Clifford, J., Chiba, H., & Chambon, P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.15.6991 · Cited by 570

Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsome

Yang, X. W., Model, P., & Heintz, N. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-859 · Cited by 544

Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation

Awatramani, R., Soriano, P., Rodriguez, C., Mai, J. J., & Dymecki, S. M. (2003) · Nature Genetics · doi:10.1038/ng1228 · Cited by 215

Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

Livet, J., Weissman, T. A., Kang, H., Draft, R. W., Lu, J., Bennis, R. A., Sanes, J. R., & Lichtman, J. W. (2007) · Nature · doi:10.1038/nature06293 · Cited by 1,876

Restoration of p53 function leads to tumour regression in vivo

Ventura, A., Kirsch, D. G., McLaughlin, M. E., Tuveson, D. A., Grimm, J., Lintault, L., Newman, J., Reczek, E. E., Weissleder, R., & Jacks, T. (2007) · Nature · doi:10.1038/nature05541 · Cited by 1,820

A conditional knockout resource for the genome-wide study of mouse gene function

Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Mujica, A. O., Thomas, M., Harrow, J., Cox, T., et al. (2011) · Nature · doi:10.1038/nature10163 · Cited by 1,708

Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs

Gong, S., Doughty, M., Harbaugh, C. R., Cummins, A., Hatten, M. E., Heintz, N., & Gerfen, C. R. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.2707-07.2007 · Cited by 977

The Endocannabinoid System Controls Key Epileptogenic Circuits in the Hippocampus

Monory, K., Massa, F., Egertová, M., Eder, M., Blaudzun, H., Westenbroek, R., Kelsch, W., Jacob, W., Marsch, R., Ekker, M., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.07.006 · Cited by 706

The role of stroma in pancreatic cancer: Diagnostic and therapeutic implications

Erkan, M., Hausmann, S., Michalski, C. W., Fingerle, A. A., Dobritz, M., Kleeff, J., & Friess, H. (2012) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2012.115 · Cited by 662

NMDA receptors, place cells and hippocampal spatial memory

Nakazawa, K., McHugh, T. J., Wilson, M. A., & Tonegawa, S. (2004) · Nature Reviews Neuroscience · doi:10.1038/nrn1385 · Cited by 619

Genetic Fate Mapping Reveals That the Caudal Ganglionic Eminence Produces a Large and Diverse Population of Superficial Cortical Interneurons

Miyoshi, G., Hjerling-Leffler, J., Karayannis, T., Sousa, V. H., Butt, S. J. B., Battiste, J., Johnson, J. E., Machold, R. P., & Fishell, G. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.4515-09.2010 · Cited by 559

Autism-associated neuroligin-3 mutations commonly impair striatal circuits to boost repetitive behaviors

Rothwell, P. E., Fuccillo, M. V., Maxeiner, S., Hayton, S. J., Gokce, O., Lim, B. K., Fowler, S. C., Malenka, R. C., & Südhof, T. C. (2014) · Cell · doi:10.1016/j.cell.2014.04.045 · Cited by 477

Faithful activation of an extra‐bright red fluorescent protein in “knock‐in” Cre‐reporter mice ideally suited for lineage tracing studies

Luche, H., Weber, O., Nageswara Rao, T., Blum, C., & Fehling, H. J. (2007) · European Journal of Immunology · doi:10.1002/eji.200636745 · Cited by 482

Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research

Moullan, N., Mouchiroud, L., Wang, X., Ryu, D., Williams, E. G., Mottis, A., Jovaisaite, V., Frochaux, M. V., Quiros, P. M., Deplancke, B., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.02.034 · Cited by 470

Animal models of major depression and their clinical implications

Czéh, B., Fuchs, E., Wiborg, O., & Simon, M. (2016) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2015.04.004 · Cited by 375

Lessons on Conditional Gene Targeting in Mouse Adipose Tissue

Lee, K. Y., Russell, S. J., Ussar, S., Boucher, J., Vernochet, C., Mori, M. A., Smyth, G., Rourk, M., Cederquist, C., Rosen, E. D., et al. (2013) · Diabetes · doi:10.2337/db12-1089 · Cited by 328

Advances in Mammalian cell line development technologies for recombinant protein production

Lai, T., Yang, Y., & Ng, S. (2013) · Pharmaceuticals · doi:10.3390/ph6050579 · Cited by 322

Building a lineage from single cells: genetic techniques for cell lineage tracking

Woodworth, M. B., Girskis, K. M., & Walsh, C. A. (2017) · Nature Reviews Genetics · doi:10.1038/nrg.2016.159 · Cited by 236

GABAergic Interneuron Lineages Selectively Sort into Specific Cortical Layers during Early Postnatal Development

Miyoshi, G., & Fishell, G. (2011) · Cerebral Cortex · doi:10.1093/cercor/bhq155 · Cited by 230

Cell-type-specific metabolic labeling of nascent proteomes in vivo

Alvarez-Castelao, B., Schanzenbächer, C. T., Hanus, C., Glock, C., tom Dieck, S., Dörrbaum, A. R., Bartnik, I., Nassim-Assir, B., Ciirdaeva, E., Mueller, A., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4016 · Cited by 219

Metabolic Pitfalls of CNS Cre-Based Technology

Harno, E., Cottrell, E. C., & White, A. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.05.019 · Cited by 200

An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase

Kranz, A., Fu, J., Duerschke, K., Weidlich, S., Naumann, R., Stewart, A. F., & Anastassiadis, K. (2010) · genesis · doi:10.1002/dvg.20641 · Cited by 153

Current issues in mouse genome engineering

Glaser, S., Anastassiadis, K., & Stewart, A. F. (2005) · Nature Genetics · doi:10.1038/ng1668 · Cited by 160

Single-neuron labeling with inducible Cre-mediated knockout in transgenic mice

Young, P., Qiu, L., Wang, D., Zhao, S., Gross, J., & Feng, G. (2008) · Nature Neuroscience · doi:10.1038/nn.2118 · Cited by 152

Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning

Sakamoto, M., Ieki, N., Miyoshi, G., Mochimaru, D., Miyachi, H., Imura, T., Yamaguchi, M., Fishell, G., Mori, K., Kageyama, R., et al. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.0674-14.2014 · Cited by 131

The Neuron Identity Problem: Form Meets Function

Fishell, G., & Heintz, N. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.035 · Cited by 115

Site-Specific Recombinases for Manipulation of the Mouse Genome

Birling, M.-C., Gofflot, F., & Warot, X. (2009) · Methods in Molecular Biology · doi:10.1007/978-1-60327-019-9_16 · Cited by 106

Mapping cell fate and function using recombinase-based intersectional strategies

Dymecki, S. M., Ray, R. S., & Kim, J. C. (2010) · Methods in Enzymology · doi:10.1016/s0076-6879(10)77011-7 · Cited by 104

Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches

Balmus, I. M., Ciobica, A., Antioch, I., Dobrin, R., & Timofte, D. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/3975101 · Cited by 103

Origins of fibrosis: pericytes take centre stage

Greenhalgh, S. N., Iredale, J. P., & Henderson, N. C. (2013) · F1000Prime Reports · doi:10.12703/p5-37 · Cited by 96

Microglia and alcohol meet at the crossroads: Microglia as critical modulators of alcohol neurotoxicity

Henriques, J. F., Portugal, C. C., Canedo, T., Relvas, J. B., Summavielle, T., & Socodato, R. (2018) · Toxicology Letters · doi:10.1016/j.toxlet.2017.11.002 · Cited by 89

Insights into parturition biology from genetically altered mice

Ratajczak, C. K., & Muglia, L. J. (2008) · Pediatric Research · doi:10.1203/pdr.0b013e31818718d2 · Cited by 83

Molecular genetics and imaging technologies for circuit-based neuroanatomy

Arenkiel, B. R., & Ehlers, M. D. (2009) · Nature · doi:10.1038/nature08536 · Cited by 78

Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors

Fischer, K. B., Collins, H. K., & Callaway, E. M. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1915974116 · Cited by 69

Drug Inducible CRISPR/Cas Systems

Zhang, J., Chen, L., Zhang, J., & Wang, Y. (2019) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2019.07.015 · Cited by 69

Extensions of MADM (mosaic analysis with double markers) in mice

Tasic, B., Miyamichi, K., Hippenmeyer, S., Dani, V. S., Zeng, H., Joo, W., Zong, H., Chen-Tsai, Y., & Luo, L. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033332 · Cited by 63

Technical approaches for mouse models of human disease

Justice, M. J., Siracusa, L. D., & Stewart, A. F. (2011) · Disease Models & Mechanisms · doi:10.1242/dmm.000901 · Cited by 66

rtTA toxicity limits the usefulness of the SP-C-rtTA transgenic mouse

Morimoto, M., & Kopan, R. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2008.10.013 · Cited by 62

Essentials of Recombinase-Based Genetic Fate Mapping in Mice

Jensen, P., & Dymecki, S. M. (2014) · Methods in Molecular Biology · doi:10.1007/978-1-60327-292-6_26 · Cited by 54

Identifying the variables that drive tamoxifen‐independent CreERT2 recombination: Implications for microglial fate mapping and gene deletions

Van Hove, H., Antunes, A. R. P., De Vlaminck, K., Scheyltjens, I., Van Ginderachter, J. A., & Movahedi, K. (2020) · European Journal of Immunology · doi:10.1002/eji.201948162 · Cited by 52

Mouse phenogenomics: the fast track to “systems metabolism

Argmann, C. A., Chambon, P., & Auwerx, J. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.11.002 · Cited by 51

HIC2 Is a Novel Dosage-Dependent Regulator of Cardiac Development Located Within the Distal 22q11 Deletion Syndrome Region

Dykes, I. M., van Bueren, K. L., Ashmore, R. J., Floss, T., Wurst, W., Szumska, D., Bhattacharya, S., & Scambler, P. J. (2014) · Circulation Research · doi:10.1161/circresaha.115.303300 · Cited by 33

Bridge protein-mediated viral targeting of cells expressing endogenous μ-opioid G protein-coupled receptors in the mouse and monkey brain

Kamaguchi, R., Amemori, S., Amemori, K.-I., & Osakada, F. (2025) · Neuroscience Research · doi:10.1016/j.neures.2025.02.007 · Cited by 2