Breastfeeding: a key modulator of gut microbiota characteristics in late infancy
Abstract
The objective of this study was to investigate the impact of the most commonly cited factors that may have influenced infants' gut microbiota profiles at one year of age: mode of delivery, breastfeeding duration and antibiotic exposure. Barcoded V3/V4 amplicons of bacterial 16S-rRNA gene were prepared from the stool samples of 52 healthy 1-year-old Australian children and sequenced using the Illumina MiSeq platform. Following the quality checks, the data were processed using the Quantitative Insights Into Microbial Ecology pipeline and analysed using the Calypso package for microbiome data analysis. The stool microbiota profiles of children still breastfed were significantly different from that of children weaned earlier (P<0.05), independent of the age of solid food introduction. Among children still breastfed, Veillonella spp. abundance was higher. Children no longer breastfed possessed a more 'mature' microbiota, with notable increases of Firmicutes. The microbiota profiles of the children could not be differentiated by delivery mode or antibiotic exposure. Further analysis based on children's feeding patterns found children who were breastfed alongside solid food had significantly different microbiota profiles compared to that of children who were receiving both breastmilk and formula milk alongside solid food. This study provided evidence that breastfeeding continues to influence gut microbial community even at late infancy when these children are also consuming table foods. At this age, any impacts from mode of delivery or antibiotic exposure did not appear to be discernible imprints on the microbial community profiles of these healthy children.
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Following birth, colonization of the infant gut is majorly determined by the early feeding mode, with distinguishable microbial compositions between breast- and formula-fed infants.
“Following birth, colonization of the infant gut is majorly determined by the early feeding mode, with distinguishable microbial compositions between breast-and formula-fed infants (254, 255).”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [255]
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Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health
Berding, K., Vlckova, K., Marx, W., Schellekens, H., Stanton, C., Clarke, G., Jacka, F., Dinan, T. G., & Cryan, J. F. (2021) · Advances in Nutrition · doi:10.1093/advances/nmaa181 · Cited by 451 · see citing works
Matsuyama, M., Morrison, M., Cao, K.-A. L., Pruilh, S., Davies, P. S. W., Wall, C., Lovell, A., & Hill, R. J. (2019) · Scientific Reports · doi:10.1038/s41598-019-48658-4 · Cited by 52 · see citing works
Self-citation
- Authors
- Matsuyama, M., Gomez-Arango, L. F., Fukuma, N. M., Morrison, M., Davies, P. S. W., & Hill, R. J.
- Affiliations
- The University of Queensland (AU)
- Obihiro University of Agriculture and Veterinary Medicine (JP)
- Translational Research Institute (AU)
- Corresponding author
- Matsuyama, M.
- Venue
- Journal of Developmental Origins of Health and Disease
- Publisher
- Cambridge University Press (CUP)
- Published
- 2018-11-19
- Volume
- 10
- Issue
- 02
- Pages
- 206-213
- ISSN
- 2040-1744 (ISSN-L)
- 2040-1752
- OpenAlex
- W2901475716
- Cited by
- 22
- References
- 55
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 6 · 2021: 4 · 2020: 1 · 2019: 5
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Developmental Origins of Health and Disease (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Health Sciences › Medicine › Epidemiology › Breastfeeding Practices and Influences
- Keywords
- breastfeeding, gut microbiota, late infancy, one-year-old children, weaning, mode of delivery, antibiotic exposure, Veillonella, Firmicutes, stool microbiome, infant feeding practices, solid food introduction, formula milk
- MeSH terms
- Firmicutes, Bacillota, Gastrointestinal Microbiome, Anti-Bacterial Agents, Australia, Breast Feeding, DNA, Bacterial, Feces, Female, Humans, Infant, Male, Milk, Human, RNA, Ribosomal, 16S, Time Factors, Veillonella, Cohort Studies, Healthy Volunteers
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