The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
Open accessAbstract
The first lab-on-chip system for picoliter droplet generation and PCR amplification with real-time fluorescence detection has performed PCR in isolated droplets at volumes 106 smaller than commercial real-time PCR instruments. The system utilized a shearing T-junction in a silicon device to generate a stream of monodisperse picoliter droplets that were isolated from the microfluidic channel walls and each other by the oil-phase carrier. An off-chip valving system stopped the droplets on-chip, allowing them to be thermally cycled through the PCR protocol without droplet motion. With this system, a 10-pL droplet, encapsulating less than one copy of viral genomic DNA through Poisson statistics, showed real-time PCR amplification curves with a cycle threshold of approximately 18, 20 cycles earlier than commercial instruments. This combination of the established real-time PCR assay with digital microfluidics is ideal for isolating single-copy nucleic acids in a complex environment.
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Metadata
- Authors
- Beer, N. R., Hindson, B. J., Wheeler, E. K., Hall, S. B., Rose, K. A., Kennedy, I. M., & Colston, B. W.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-10-11
- Volume
- 79
- Issue
- 22
- Pages
- 8471-8475
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2128839883
- Cited by
- 358
- References
- 17
- FWCI
- 18.06
- Type
- journal article
- Language
- en
- Open access
- green
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