Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines

Direct Gene Transfer into Mouse Muscle in Vivo

Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · see citing works

Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8

Heil, F., Hemmi, H., Hochrein, H., Ampenberger, F., Kirschning, C., Akira, S., Lipford, G., Wagner, H., & Bauer, S. (2004) · Science · doi:10.1126/science.1093620 · Cited by 3,947 · see citing works

Dynamic RNA Modifications in Gene Expression Regulation

Roundtree, I. A., Evans, M., Pan, T., & He, C. (2017) · Cell · doi:10.1016/j.cell.2017.05.045 · Cited by 3,456 · see citing works

Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA

Karikó, K., Buckstein, M., Ni, H., & Weissman, D. (2005) · Immunity · doi:10.1016/j.immuni.2005.06.008 · Cited by 2,728 · see citing works

Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA

Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y.-H., Li, H., Lau, F., Ebina, W., Mandal, P. K., Smith, Z. D., Meissner, A., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.012 · Cited by 2,662 · see citing works

mRNA-based therapeutics--developing a new class of drugs

Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474 · see citing works

Structural and functional properties of SARS-CoV-2 spike protein: potential antivirus drug development for COVID-19

Huang, Y., Yang, C., Xu, X.-F., Xü, W., & Liu, S. (2020) · Acta Pharmacologica Sinica · doi:10.1038/s41401-020-0485-4 · Cited by 2,207 · see citing works

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

Karikó, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., & Weissman, D. (2008) · Molecular Therapy · doi:10.1038/mt.2008.200 · Cited by 1,930 · see citing works

Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers

Shi, H., Wei, J., & He, C. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.04.025 · Cited by 1,915 · see citing works

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes

Hsieh, C.-L., Goldsmith, J. A., Schaub, J. M., DiVenere, A. M., Kuo, H.-C., Javanmardi, K., Le, K. C., Wrapp, D., Lee, A. G., Liu, Y., et al. (2020) · Science · doi:10.1126/science.abd0826 · Cited by 1,303 · see citing works

[5] Synthesis of small RNAs using T7 RNA polymerase

Milligan, J. F., & Uhlenbeck, O. C. (1989) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(89)80091-6 · Cited by 1,089 · see citing works

mRNA is an endogenous ligand for toll-like receptor 3

Karikó, K., Ni, H., Capodici, J., Lamphier, M., & Weissman, D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310175200 · Cited by 1,062 · see citing works

Cationic liposome-mediated RNA transfection.

Malone, R. W., Felgner, P. L., & Verma, I. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6077 · Cited by 911 · see citing works

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

Karikó, K., Muramatsu, H., Ludwig, J., & Weissman, D. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr695 · Cited by 898 · see citing works

Rapid COVID-19 vaccine development

Graham, B. S. (2020) · Science · doi:10.1126/science.abb8923 · Cited by 771 · see citing works

Regulation of translation via mRNA structure in prokaryotes and eukaryotes

Kozak, M. (2005) · Gene · doi:10.1016/j.gene.2005.06.037 · Cited by 747 · see citing works

Expression of therapeutic proteins after delivery of chemically modified mRNA in mice

Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733 · see citing works

N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice

Andries, O., Mc Cafferty, S., De Smedt, S. C., Weiss, R., Sanders, N. N., & Kitada, T. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.051 · Cited by 701 · see citing works

BNT162b vaccines protect rhesus macaques from SARS-CoV-2

Vogel, A. B., Kanevsky, I., Che, Y., Swanson, K. A., Muik, A., Vormehr, M., Kranz, L. M., Walzer, K. C., Hein, S., Güler, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03275-y · Cited by 694 · see citing works

Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation

Anderson, B. R., Muramatsu, H., Nallagatla, S. R., Bevilacqua, P. C., Sansing, L. H., Weissman, D., & Karikó, K. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq347 · Cited by 682 · see citing works

On the formation and morphology of lipid nanoparticles containing ionizable cationic lipids and siRNA

Kulkarni, J. A., Darjuan, M. M., Mercer, J. E., Chen, S., van der Meel, R., Thewalt, J. L., Tam, Y. Y. C., & Cullis, P. R. (2018) · ACS Nano · doi:10.1021/acsnano.8b01516 · Cited by 663 · see citing works

RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP)

Siegfried, N. A., Busan, S., Rice, G. M., Nelson, J. A. E., & Weeks, K. M. (2014) · Nature Methods · doi:10.1038/nmeth.3029 · Cited by 662 · see citing works

The emerging role of RNA modifications in the regulation of mRNA stability

Boo, S. H., & Kim, Y. K. (2020) · Experimental & Molecular Medicine · doi:10.1038/s12276-020-0407-z · Cited by 588 · see citing works

mRNA structure regulates protein expression through changes in functional half-life

Mauger, D. M., Cabral, B. J., Presnyak, V., Su, S. V., Reid, D. W., Goodman, B., Link, K., Khatwani, N., Reynders, J., Moore, M. J., et al. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1908052116 · Cited by 555 · see citing works

New RNA Polymerase from Escherichia coli infected with Bacteriophage T7

Chamberlin, M. J., MCGRATH, J., & Waskell, L. A. (1970) · Nature · doi:10.1038/228227a0 · Cited by 545 · see citing works

A noninflammatory mRNA vaccine for treatment of experimental autoimmune encephalomyelitis

Krienke, C., Kolb, L., Diken, E., Streuber, M., Kirchhoff, S., Bukur, T., Akilli-Öztürk, Ö., Kranz, L. M., Berger, H., Petschenka, J., et al. (2021) · Science · doi:10.1126/science.aay3638 · Cited by 520 · see citing works

Sequence-engineered mRNA without chemical nucleoside modifications enables an effective protein therapy in large animals

Thess, A., Grund, S., Mui, B. L., Hope, M. J., Baumhof, P., Fotin-Mleczek, M., & Schlake, T. (2015) · Molecular Therapy · doi:10.1038/mt.2015.103 · Cited by 513 · see citing works

Recent advances in mRNA vaccine technology

Pardi, N., Hogan, M. J., & Weissman, D. (2020) · Current Opinion in Immunology · doi:10.1016/j.coi.2020.01.008 · Cited by 512 · see citing works

Human 5′ UTR design and variant effect prediction from a massively parallel translation assay

Sample, P. J., Wang, B., Reid, D. W., Presnyak, V., McFadyen, I. J., Morris, D. R., & Seelig, G. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0164-5 · Cited by 442 · see citing works

Stabilization of RNA stacking by pseudouridine

Davis, D. R. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.24.5020 · Cited by 399 · see citing works

Tailoring mRNA Vaccine to Balance Innate/Adaptive Immune Response

Linares-Fernández, S., Lacroix, C., Exposito, J.-Y., & Verrier, B. (2020) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2019.10.002 · Cited by 376 · see citing works

Improving mRNA-Based Therapeutic Gene Delivery by Expression-Augmenting 3′ UTRs Identified by Cellular Library Screening

Orlandini von Niessen, A. G., Poleganov, M. A., Rechner, C., Plaschke, A., Kranz, L. M., Fesser, S., Diken, M., Löwer, M., Vallazza, B., Beissert, T., et al. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2018.12.011 · Cited by 365 · see citing works

Impact of mRNA chemistry and manufacturing process on innate immune activation

Nelson, J., Sorensen, E. W., Mintri, S., Rabideau, A. E., Zheng, W., Besin, G., Khatwani, N., Su, S. V., Miracco, E. J., Issa, W. J., et al. (2020) · Science Advances · doi:10.1126/sciadv.aaz6893 · Cited by 354 · see citing works

COVID-19 Vaccine Frontrunners and Their Nanotechnology Design

Chung, Y. H., Beiss, V., Fiering, S., & Steinmetz, N. F. (2020) · ACS Nano · doi:10.1021/acsnano.0c07197 · Cited by 341 · see citing works

Control of mammalian translation by mRNA structure near caps

Babendure, J. R., Babendure, J. L., Ding, J.-H., & Tsien, R. Y. (2006) · RNA · doi:10.1261/rna.2309906 · Cited by 339 · see citing works

Nucleic acid agonists for Toll‐like receptor 7 are defined by the presence of uridine ribonucleotides

Diebold, S. S., Massacrier, C., Akira, S., Paturel, C., Morel, Y., & Sousa, C. R. E. (2006) · European Journal of Immunology · doi:10.1002/eji.200636617 · Cited by 281 · see citing works

N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density

Svitkin, Y. V., Cheng, Y. M., Chakraborty, T., Presnyak, V., John, M., & Sonenberg, N. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx135 · Cited by 278 · see citing works

The contribution of pseudouridine to stabilities and structure of RNAs

Kierzek, E., Malgowska, M., Lisowiec, J., Turner, D. H., Gdaniec, Z., & Kierzek, R. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1330 · Cited by 264 · see citing works

Systemic Messenger RNA Therapy as a Treatment for Methylmalonic Acidemia

An, D., Schneller, J. L., Frassetto, A., Liang, S., Zhu, X., Park, J.-S., Theisen, M., Hong, S.-J., Zhou, J., Rajendran, R., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.081 · Cited by 263 · see citing works

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

Vaidyanathan, S., Azizian, K. T., Haque, A. K. M. A., Henderson, J. M., Hendel, A., Shore, S., Antony, J. S., Hogrefe, R. I., Kormann, M. S. D., Porteus, M. H., et al. (2018) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2018.06.010 · Cited by 262 · see citing works

Advances in Lipid Nanoparticles for mRNA-Based Cancer Immunotherapy

Guevara, M. L., Persano, F., & Persano, S. (2020) · Frontiers in Chemistry · doi:10.3389/fchem.2020.589959 · Cited by 257 · see citing works

RNAs containing modified nucleotides fail to trigger RIG-I conformational changes for innate immune signaling

Durbin, A. F., Wang, C., Marcotrigiano, J., & Gehrke, L. (2016) · mBio · doi:10.1128/mbio.00833-16 · Cited by 238 · see citing works

Cap 1 Messenger RNA Synthesis with Co‐transcriptional CleanCap ^® Analog by In Vitro Transcription

Henderson, J. M., Ujita, A., Hill, E., Yousif‐Rosales, S., Smith, C., Ko, N., McReynolds, T., Cabral, C. R., Escamilla‐Powers, J. R., & Houston, M. E. (2021) · Current Protocols · doi:10.1002/cpz1.39 · Cited by 231 · see citing works

An RNA toolbox for cancer immunotherapy

Pastor, F., Berraondo, P., Etxeberria, I., Frederick, J., Sahin, U., Gilboa, E., & Melero, I. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2018.132 · Cited by 229 · see citing works

Pseudouridinylation of mRNA coding sequences alters translation

Eyler, D. E., Franco, M. K., Batool, Z., Wu, M. Z., Dubuke, M. L., Dobosz-Bartoszek, M., Jones, J. D., Polikanov, Y. S., Roy, B., & Koutmou, K. S. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1821754116 · Cited by 227 · see citing works

Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

Wróblewska, L., Kitada, T., Endo, K., Siciliano, V., Stillo, B., Saito, H., & Weiss, R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3301 · Cited by 224 · see citing works

RNA-Based Vaccines in Cancer Immunotherapy

McNamara, M. A., Nair, S. K., & Holl, E. K. (2015) · Journal of Immunology Research · doi:10.1155/2015/794528 · Cited by 223 · see citing works

An origin of the immunogenicity of in vitro transcribed RNA

Mu, X., Greenwald, E., Ahmad, S., & Hur, S. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky177 · Cited by 218 · see citing works

Efficacy and immunogenicity of unmodified and pseudouridine-modified mRNA delivered systemically with lipid nanoparticles in vivo

Kauffman, K. J., Mir, F. F., Jhunjhunwala, S., Kaczmarek, J. C., Hurtado, J. E., Yang, J. H., Webber, M. J., Kowalski, P. S., Heartlein, M. W., DeRosa, F., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.09.006 · Cited by 191 · see citing works

Learning from the past: development of safe and effective COVID-19 vaccines

Su, S., Du, L., & Jiang, S. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-00462-y · Cited by 170 · see citing works

mRNA structure determines modification by pseudouridine synthase 1

Carlile, T. M., Martínez, N. M., Schaening-Burgos, C., Su, A., Bell, T. A., Zinshteyn, B., & Gilbert, W. V. (2019) · Nature Chemical Biology · doi:10.1038/s41589-019-0353-z · Cited by 162 · see citing works

Effects of Chemically Modified Messenger RNA on Protein Expression

Li, B., Luo, X. L., & Dong, Y. (2016) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.6b00090 · Cited by 154 · see citing works

Designing liposomal adjuvants for the next generation of vaccines

Perrie, Y., Crofts, F. J., Devitt, A., Griffiths, H. R., Kastner, E., & Nadella, V. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.11.005 · Cited by 139 · see citing works

COVID-19 and mRNA Vaccines—First Large Test for a New Approach

Abbasi, J. (2020) · JAMA · doi:10.1001/jama.2020.16866 · Cited by 132 · see citing works

Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life

Trepotec, Z., Geiger, J., Plank, C., Aneja, M. K., & Rudolph, C. (2019) · RNA · doi:10.1261/rna.069286.118 · Cited by 129 · see citing works

Optimization of mRNA untranslated regions for improved expression of therapeutic mRNA

Asrani, K. H., Farelli, J. D., Stahley, M. R., Miller, R. L., Cheng, C. J., Subramanian, R. R., & Brown, J. M. (2018) · RNA Biology · doi:10.1080/15476286.2018.1450054 · Cited by 111 · see citing works

[34] Isolation of bacterial and bacteriophage RNA polymerases and their use in synthesis of RNA in Vitro

Chamberlin, M. J., Kingston, R., Gilman, M. Z., et al. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)01037-x · Cited by 109 · see citing works

Liposomal adjuvants for human vaccines

Alving, C. R., Beck, Z., Matyas, G. R., & Rao, M. (2016) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2016.1151871 · Cited by 105 · see citing works

Profiling Cytidine Acetylation with Specific Affinity and Reactivity

Sinclair, W. R., Arango, D., Shrimp, J. H., Zengeya, T. T., Thomas, J. M., Montgomery, D. C., Fox, S. D., Andrésson, Þ., Oberdoerffer, S., & Meier, J. L. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00734 · Cited by 83 · see citing works

Messenger RNA Structure Regulates Translation Initiation: A Mechanism Exploited from Bacteria to Humans

Mustoe, A. M., Corley, M., Laederach, A., et al. (2018) · Biochemistry · doi:10.1021/acs.biochem.8b00395 · Cited by 61 · see citing works

Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates

Milisavljevič, N., Perlíková, P., Pohl, R., et al. (2018) · Organic & Biomolecular Chemistry · doi:10.1039/c8ob01498a · Cited by 49 · see citing works

P(V) Reagents for the Scalable Synthesis of Natural and Modified Nucleoside Triphosphates

Liao, J.-Y., Bala, S., Ngor, A. K., Yik, E. J., & Chaput, J. C. (2019) · Journal of the American Chemical Society · doi:10.1021/jacs.9b04728 · Cited by 44 · see citing works

Synthesis and solution conformation studies of 3-substituted uridine and pseudouridine derivatives

Chang, Y., Herath, J., Wang, T. H.-H., et al. (2007) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2007.11.039 · Cited by 43 · see citing works

The incorporation of 5-ribosyluracil triphosphate into RNA in nuclear extracts of mammalian cells

GOLDBERG, I. H., & Rabinowitz, M. (1961) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(61)90152-8 · Cited by 40 · see citing works

Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

Kimoto, M., Meyer, A. J., Hirao, I., et al. (2017) · Chemical Communications · doi:10.1039/c7cc06661a · Cited by 26 · see citing works

The virosomal adjuvanted influenza vaccine

Calcagnile, S., & Zuccotti, G. (2010) · Expert Opinion on Biological Therapy · doi:10.1517/14712590903431014 · Cited by 25 · see citing works

Systemic Messenger RNA Therapy as a Treatment for Methylmalonic Acidemia

An, D., Schneller, J. L., Frassetto, A., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.08.049 · Cited by 25 · see citing works

Comparative Utilization of Pseudouridine Triphosphate and Uridine Triphosphate by Ribonucleic Acid Polymerase

GOLDBERG, I. H., & Rabinowitz, M. (1963) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81139-5 · Cited by 21 · see citing works

Enzymatic incorporation and utilization of an emissive 6-azauridine

Hopkins, P. A., McCoy, L. S., & Tor, Y. (2016) · Organic & Biomolecular Chemistry · doi:10.1039/c6ob02080a · Cited by 10 · see citing works

Advances in COVID-19 mRNA vaccine development

Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works

Principles for designing an optimal mRNA lipid nanoparticle vaccine

Kon, E., Elia, U., & Peer, D. (2022) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.09.016 · Cited by 269 · see citing works

СТРУКТУРНО-КОМПОЗИЦІЙНІ ОСОБЛИВОСТІ ДИСКУРСИВНОГО РОЗГОРТАННЯ НАРАТИВУ “CORONAVIRUS PANDEMIC” (НА МАТЕРІАЛІ ІНФОРМАЦІЙНОГО ПОРТАЛУ BBC NEWS)

Aleksenko, S., & Приходько, М. В. (2021) · Collection of scientific works Visnyk of Zaporizhzhya National University Philological Sciences · doi:10.26661/2414-9594-2021-1-1

Confirmation of SARS-CoV-2 airborne dissemination indoors using “COVID-19 traps”

Orenes‐Piñero, E., Navas-Carrillo, D., Moreno‐Docón, A., Ortega-García, J. A., Torres-Cantero, A. M., García‐Vázquez, E., & Ramírez, P. (2021) · Journal of Infection · doi:10.1016/j.jinf.2021.12.017 · Cited by 4 · see citing works

Fachinformationen zu SARS-CoV-2/COVID-19

(2020) · RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren · doi:10.1055/a-1154-3939

And now for something completely different: from 2019-nCoV and COVID-19 to 2020-nMan

Froes, F. (2020) · Pulmonology · doi:10.1016/j.pulmoe.2020.02.010 · Cited by 25 · see citing works

Realizing the Potential of Anti–SARS-CoV-2 Monoclonal Antibodies for COVID-19 Management

Li, J. Z., & Gandhi, R. T. (2022) · JAMA · doi:10.1001/jama.2021.19994 · Cited by 20 · see citing works