Cited by 177Luminescence and Fluorescent MaterialsNonlinear Optical Materials StudiesAdvanced Fluorescence Microscopy Techniques

Aggregation-Induced Emission Luminogen with Deep-Red Emission for Through-Skull Three-Photon Fluorescence Imaging of Mouse

Wang, Yalun, Chen, Ming, Alifu, Nuernisha, Li, Shiwu, Qin, Wei, Qin, Anjun, Tang, Ben Zhong, Qian, Jun·2017·ACS Nano

Abstract

Imaging the brain with high integrity is of great importance to neuroscience and related applications. X-ray computed tomography (CT) and magnetic resonance imaging (MRI) are two clinically used modalities for deep-penetration brain imaging. However, their spatial resolution is quite limited. Two-photon fluorescence microscopic (2PFM) imaging with its femtosecond (fs) excitation wavelength in the traditional near-infrared (NIR) region (700-1000 nm) is able to realize deep-tissue and high-resolution brain imaging. However, it requires craniotomy and cranial window or skull-thinning techniques due to photon scattering of the excitation light. Herein, based on a type of aggregation-induced emission luminogen (AIEgen) DCDPP-2TPA with a large three-photon absorption (3PA) cross section at 1550 nm and deep-red emission, we realized through-skull three-photon fluorescence microscopic (3PFM) imaging of mouse cerebral vasculature without craniotomy and skull-thinning. Reduced photon scattering of a 1550 nm fs excitation laser allowed it to effectively penetrate the skull and tightly focus onto DCDPP-2TPA nanoparticles (NPs) in the cerebral vasculature, generating bright three-photon fluorescence (3PF) signals. In vivo 3PF images of the cerebral vasculature at various vertical depths were obtained, and a vivid 3D reconstruction of the vascular architecture beneath the skull was built. As deep as 300 μm beneath the skull, small blood vessels of 2.4 μm could still be recognized.

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Crossref · 10.1021/acsnano.7b05645

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Authors
Wang, Y., Chen, M., Alifu, N., Li, S., Qin, W., Qin, A., Tang, B. Z., & Qian, J.
Affiliations
  • Zhejiang Normal University (CN)
  • Hong Kong University of Science and Technology (HK)
  • State Key Laboratory of Luminescent Materials and Devices (CN)
Corresponding author
  • Qin, Anjun
  • Tang, Ben Zhong
  • Qian, Jun
Venue
ACS Nano
Published
2017-10-12
Volume
11
Issue
10
Pages
10452-10461
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
OpenAlex
W2762534712
Cited by
177
References
43
Citations by year
2026: 6 · 2025: 9 · 2024: 14 · 2023: 9 · 2022: 35 · 2021: 25 · 2020: 24 · 2019: 30 · 2018: 25
FWCI
9.12
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Nano (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Funders
  • Research Grants Council, University Grants Committee (16305015, 16308016, N-HKUST604/14)
  • Ministry of Science and Technology of the People's Republic of China (2013CB834701, 2013CB834704, 2015AA020515)
  • University Grants Committee (AoE/P-03/08)
  • Innovation and Technology Commission (ITC-CNERC14SC01)
  • Natural Science Foundation of Zhejiang Province (LR17F050001)
  • Shenzhen Municipality (JCYJ20160229205601482)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
  • Physical Sciences › Engineering › Biomedical Engineering › Nonlinear Optical Materials Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
aggregation-induced emission, deep-red emission, cerebrovascular imaging, 1550 nm excitation, three-photon absorption, nanoparticle contrast agents, in vivo brain imaging, 3D vascular reconstruction
MeSH terms
Animals, Brain, Female, Fluorescent Dyes, Mice, Inbred BALB C, Microscopy, Fluorescence, Particle Size, Surface Properties, Molecular Structure, Photons, Mice, Nanoparticles, Optical Imaging

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