The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Induction of Apoptosis in Cancer Cells at Low Silver Nanoparticle Concentrations using Chitosan Nanocarrier
Abstract
We report the development of a chitosan nanocarrier (NC)-based delivery of silver nanoparticles (Ag NPs) to mammalian cells for induction of apoptosis at very low concentrations of the NPs. The cytotoxic efficacy of the Ag NP-nanocarrier (Ag-CS NC) system in human colon cancer cells (HT 29) was examined by morphological analyses and biochemical assays. Cell viability assay demonstrated that the concentration of Ag NPs required to reduce the viability of HT 29 cells by 50% was 0.33 μg mL(-1), much less than in previously reported data. The efficient induction of apoptosis by Ag-CS NCs was confirmed by flow cytometry. Additionally, the characteristic nuclear and morphological changes during apoptotic cell death were investigated by fluorescence and scanning electron microscopy (SEM), respectively. The involvement of mitochondrial pathway of cell death in the Ag-CS NCs induced apoptosis was evident from the depolarization of mitochondrial membrane potential (ΔΨ(m)). Real time quantitative RT-PCR analysis demonstrated the up-regulation of caspase 3 expression which was further reflected in the formation of oligo-nucleosomal DNA "ladders" in Ag-CS NCs treated cells, indicating the important role of caspases in the present apoptotic process. The increased production of intracellular ROS due to Ag-CS NCs treatment indicated that the oxidative stress could augment the induction of apoptosis in HT 29 cells in addition to classical caspase signaling pathway. The use of significantly low concentration of Ag NPs impregnated in chitosan nanocarrier is a much superior approach in comparison to the use of free Ag NPs in cancer therapy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
1 retracted
Retracted: Characterization of enhanced antibacterial effects of novel silver nanoparticles
Shrivastava, S., Bera, T., Roy, A., Singh, G., Ramachandrarao, P., & Dash, D. (2007) · Nanotechnology · doi:10.1088/0957-4484/18/22/225103 · Cited by 1,523
RetractedNanocarriers as an emerging platform for cancer therapy
Peer, D., Karp, J. M., Hong, S., Farokhzad, O. C., Margalit, R., & Langer, R. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.387 · Cited by 8,710
The bactericidal effect of silver nanoparticles
Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K., Kouri, J. B., Ramírez, J. T., & Yacaman, M. J. (2005) · Nanotechnology · doi:10.1088/0957-4484/16/10/059 · Cited by 6,668
Apoptosis in the Pathogenesis and Treatment of Disease
Thompson, C. B. (1995) · Science · doi:10.1126/science.7878464 · Cited by 6,524
Sondi, I., & Salopek-Sondi, B. (2004) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2004.02.012 · Cited by 5,986
Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold Nanorods
Huang, X., El-Sayed, I. H., Qian, W., & El-Sayed, M. A. (2006) · Journal of the American Chemical Society · doi:10.1021/ja057254a · Cited by 5,436
Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
Silver nanoparticles: Green synthesis and their antimicrobial activities
Sharma, V. K., Yngard, R. A., & Lin, Y. (2009) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2008.09.002 · Cited by 3,900
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
AshaRani, P. V., Low Kah Mun, G., Hande, M. P., & Valiyaveettil, S. (2009) · ACS Nano · doi:10.1021/nn800596w · Cited by 3,692
Chitosan Chemistry and Pharmaceutical Perspectives
Kumar, M. N. V. R., Muzzarelli, R. A. A., Muzzarelli, C., Sashiwa, H., & Domb, A. J. (2004) · Chemical Reviews · doi:10.1021/cr030441b · Cited by 2,938
Martin, S. J., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., van Schie, R. C., LaFace, D. M., & Green, D. R. (1995) · The Journal of experimental medicine · doi:10.1084/jem.182.5.1545 · Cited by 2,824
Koopman, G., Reutelingsperger, C., Kuijten, G., Keehnen, R., Pals, S., & van Oers, M. (1994) · Blood · doi:10.1182/blood.v84.5.1415.1415 · Cited by 2,681
Magnetic nanoparticles in MR imaging and drug delivery
Sun, C., Lee, J. S. H., & Zhang, M. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.03.018 · Cited by 2,594
Chitosan-based hydrogels for controlled, localized drug delivery
Bhattarai, N., Gunn, J., & Zhang, M. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.07.019 · Cited by 2,451
In vitro toxicity of nanoparticles in BRL 3A rat liver cells
Hussain, S. M., Hess, K. L., Gearhart, J. M., Geiss, K. T., & Schlager, J. J. (2005) · Toxicology in Vitro · doi:10.1016/j.tiv.2005.06.034 · Cited by 2,025
Mitochondria, oxidative stress and cell death
Ott, M., Gogvadze, V., Orrenius, S., & Zhivotovsky, B. (2007) · Apoptosis · doi:10.1007/s10495-007-0756-2 · Cited by 1,977
Caspase-3 Is Required for DNA Fragmentation and Morphological Changes Associated with Apoptosis
Jänicke, R. U., Sprengart, M. L., Wati, M. R., & Porter, A. G. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.16.9357 · Cited by 1,953
Artificially engineered magnetic nanoparticles for ultra-sensitive molecular imaging
Lee, J.-H., Huh, Y.-M., Jun, Y.-W., Seo, J.-W., Jang, J.-T., Song, H.-T., Kim, S., Cho, E.-J., Yoon, H.-G., Suh, J.-S., et al. (2007) · Nature Medicine · doi:10.1038/nm1467 · Cited by 1,897
Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers
Calvo, P., Remu��n-L�pez, C., Vila-Jato, J. L., & Alonso, M. J. (1997) · Journal of Applied Polymer Science · doi:10.1002/(sici)1097-4628(19970103)63:1<125::aid-app13>3.0.co;2-4 · Cited by 1,856
Doxorubicin-Induced Cardiomyopathy
Singal, P. K., & Iliskovic, N. (1998) · New England Journal of Medicine · doi:10.1056/nejm199809243391307 · Cited by 1,845
Immunotargeted Nanoshells for Integrated Cancer Imaging and Therapy
Loo, C., Lowery, A., Halas, N., West, J., & Drezek, R. (2005) · Nano Letters · doi:10.1021/nl050127s · Cited by 1,792
Newmeyer, D. D., & Ferguson-Miller, S. (2003) · Cell · doi:10.1016/s0092-8674(03)00116-8 · Cited by 1,269
The "bystander effect": tumor regression when a fraction of the tumor mass is genetically modified.
Freeman, S. M., Abboud, C. N., Whartenby, K. A., Packman, C. H., Koeplin, D. S., Moolten, F. L., & Abraham, G. N. (1993) · PubMed · Cited by 1,084
Toxicity of silver nanoparticles in zebrafish models
Asharani, P. V., Lian Wu, Y., Gong, Z., & Valiyaveettil, S. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/25/255102 · Cited by 1,062
Computationally Guided Photothermal Tumor Therapy Using Long-Circulating Gold Nanorod Antennas
von Maltzahn, G., Park, J.-H., Agrawal, A., Bandaru, N. K., Das, S. K., Sailor, M. J., & Bhatia, S. N. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-4242 · Cited by 1,051
Caspases: the proteases of the apoptotic pathway
Nuñez, G., Benedict, M. A., Hu, Y., & Inohara, N. (1998) · Oncogene · doi:10.1038/sj.onc.1202581 · Cited by 1,046
Cossarizza, A., Baccaranicontri, M., Kalashnikova, G., & Franceschi, C. (1993) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1993.2438 · Cited by 1,029
Potential of silver nanoparticle‐coated polyurethane foam as an antibacterial water filter
Jain, P., & Pradeep, T. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20368 · Cited by 934
Iron Oxide Nanoparticles for Sustained Delivery of Anticancer Agents
Jain, T. K., Morales, M. A., Sahoo, S. K., Leslie-Pelecky, D. L., & Labhasetwar, V. (2005) · Molecular Pharmaceutics · doi:10.1021/mp0500014 · Cited by 904
Limbach, L. K., Wick, P., Manser, P., Grass, R. N., Bruinink, A., & Stark, W. J. (2007) · Environmental Science & Technology · doi:10.1021/es062629t · Cited by 889
Hsin, Y.-H., Chen, C.-F., Huang, S., Shih, T.-S., Lai, P.-S., & Chueh, P. J. (2008) · Toxicology Letters · doi:10.1016/j.toxlet.2008.04.015 · Cited by 864
Cellular responses induced by silver nanoparticles: In vitro studies
Arora, S., Jain, J., Rajwade, J. M., & Paknikar, K. M. (2008) · Toxicology Letters · doi:10.1016/j.toxlet.2008.04.009 · Cited by 736
Nano-C60 cytotoxicity is due to lipid peroxidation
Sayes, C. M., Gobin, A. M., Ausman, K. D., Mendez, J., West, J. L., & Colvin, V. L. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.05.027 · Cited by 686
Jain, J., Arora, S., Rajwade, J. M., Omray, P., Khandelwal, S., & Paknikar, K. M. (2009) · Molecular Pharmaceutics · doi:10.1021/mp900056g · Cited by 629
Kohler, N., Sun, C., Wang, J., & Zhang, M. (2005) · Langmuir · doi:10.1021/la0503451 · Cited by 579
Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells
Park, E.-J., Choi, J., Park, Y.-K., & Park, K. (2008) · Toxicology · doi:10.1016/j.tox.2007.12.022 · Cited by 565
Sahu, A., Kasoju, N., & Bora, U. (2008) · Biomacromolecules · doi:10.1021/bm800683f · Cited by 548
Targeted Photothermal Lysis of the Pathogenic Bacteria, Pseudomonas aeruginosa, with Gold Nanorods
Norman, R. S., Stone, J. W., Gole, A., Murphy, C. J., & Sabo-Attwood, T. L. (2008) · Nano Letters · doi:10.1021/nl0727056 · Cited by 503
Cellular localisation of a water-soluble fullerene derivative
Foley, S., Crowley, C., Smaihi, M., Bonfils, C., Erlanger, B. F., Seta, P., & Larroque, C. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)00445-x · Cited by 492
New fluorescent dyes for lymphocyte migration studies
Weston, S. A., & Parish, C. R. (1990) · Journal of Immunological Methods · doi:10.1016/0022-1759(90)90322-m · Cited by 484
Morphological and biochemical characterization and analysis of apoptosis
Allen, R. T., Hunter, W. J., & Agrawal, D. K. (1997) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/s1056-8719(97)00033-6 · Cited by 477
Implication of Mitochondrial Hydrogen Peroxide Generation in Ceramide-induced Apoptosis
Quillet-Mary, A., Jaffrézou, J.-P., Mansat, V., Bordier, C., Naval, J., & Laurent, G. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.34.21388 · Cited by 476
Self-Assembly of Ordered, Robust, Three-Dimensional Gold Nanocrystal/Silica Arrays
Fan, H., Yang, K., Boye, D. M., Sigmon, T., Malloy, K. J., Xu, H., López, G. P., & Brinker, C. J. (2004) · Science · doi:10.1126/science.1095140 · Cited by 473
Gogoi, S. K., Gopinath, P., Paul, A., Ramesh, A., Ghosh, S. S., & Chattopadhyay, A. (2006) · Langmuir · doi:10.1021/la060661v · Cited by 446
Self-citationThe antibacterial properties of a novel chitosan–Ag-nanoparticle composite
Sanpui, P., Murugadoss, A., Prasad, P. V. D., Ghosh, S. S., & Chattopadhyay, A. (2008) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2008.03.004 · Cited by 397
Self-citationSemiconductor quantum dots and free radical induced DNA nicking
Green, M., & Howman, E. (2005) · Chemical Communications · doi:10.1039/b413175d · Cited by 323
Rivera Gil, P., Oberdörster, G., Elder, A., Puntes, V., & Parak, W. J. (2010) · ACS Nano · doi:10.1021/nn1025687 · Cited by 314
Murugadoss, A., & Chattopadhyay, A. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/01/015603 · Cited by 308
Self-citationDreis, S., Rothweiler, F., Michaelis, M., Cinatl, J., Kreuter, J., & Langer, K. (2007) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2007.03.036 · Cited by 275
Yang, S.-J., Lin, F.-H., Tsai, K.-C., Wei, M.-F., Tsai, H.-M., Wong, J.-M., & Shieh, M.-J. (2010) · Bioconjugate Chemistry · doi:10.1021/bc9004798 · Cited by 272
Paclitaxel-Loaded Gelatin Nanoparticles for Intravesical Bladder Cancer Therapy
Lu, Z., Yeh, T.-K., Tsai, M., Au, J. L.-S., & Wientjes, M. G. (2004) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-04-1443 · Cited by 256
Signaling gene cascade in silver nanoparticle induced apoptosis
Gopinath, P., Gogoi, S. K., Sanpui, P., Paul, A., Chattopadhyay, A., & Ghosh, S. S. (2010) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2010.01.033 · Cited by 225
Self-citationImplications of silver nanoparticle induced cell apoptosis for in vitro gene therapy
Gopinath, P., Gogoi, S. K., Chattopadhyay, A., & Ghosh, S. S. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/7/075104 · Cited by 202
Self-citationBanerjee, M., Mallick, S., Paul, A., Chattopadhyay, A., & Ghosh, S. S. (2010) · Langmuir · doi:10.1021/la9038528 · Cited by 196
Self-citationThubagere, A., & Reinhard, B. M. (2010) · ACS Nano · doi:10.1021/nn100389a · Cited by 163
Wataha, J. C., Lockwood, P. E., & Schedle, A. (2000) · Journal of Biomedical Materials Research · Cited by 120
The side effects of chemotherapeutic agents
Carr, C., Ng, J., & Wigmore, T. (2008) · Current Anaesthesia and Critical Care · doi:10.1016/j.cacc.2008.01.004 · Cited by 107
Mitochondria at the Crossroad of Apoptotic Cell Death
Thress, K. S., Kornbluth, S. A., & Smith, J. J. (1999) · Journal of Bioenergetics and Biomembranes · doi:10.1023/a:1005471701441 · Cited by 50
Gopinath, P., & Ghosh, S. S. (2008) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-008-9980-5 · Cited by 16
Self-citation
Cited by
Zhang, X.-F., Liu, Z.-G., Shen, W., & Gurunathan, S. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17091534 · Cited by 3,220
Reddy, N. J., Nagoor Vali, D., Rani, M., & Rani, S. S. (2014) · Materials Science and Engineering: C · doi:10.1016/j.msec.2013.08.039 · Cited by 379
Kalaivani, R., Maruthupandy, M., Muneeswaran, T., Hameedha Beevi, A., Anand, M., Ramakritinan, C. M., & Kumaraguru, A. K. (2018) · Frontiers in Laboratory Medicine · doi:10.1016/j.flm.2018.04.002 · Cited by 336
Sabaratnam, V., Gurunathan, S., Raman, J., Abd Malek, S. N., & John, P. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s51881 · Cited by 301
Singh, H., Du, J., Singh, P., & Yi, T. H. (2018) · Artificial Cells, Nanomedicine, and Biotechnology · doi:10.1080/21691401.2017.1362417 · Cited by 257
Metadata
- Authors
- Sanpui, P., Chattopadhyay, A., & Ghosh, S. S.
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-01-31
- Volume
- 3
- Issue
- 2
- Pages
- 218-228
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2321369253
- Cited by
- 417
- References
- 59
- FWCI
- 11.31
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
