The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Regulation of Silk Material Structure by Temperature-Controlled Water Vapor Annealing
Open accessAbstract
We present a simple and effective method to obtain refined control of the molecular structure of silk biomaterials through physical temperature-controlled water vapor annealing (TCWVA). The silk materials can be prepared with control of crystallinity, from a low content using conditions at 4 °C (α helix dominated silk I structure), to highest content of ∼60% crystallinity at 100 °C (β-sheet dominated silk II structure). This new physical approach covers the range of structures previously reported to govern crystallization during the fabrication of silk materials, yet offers a simpler, green chemistry, approach with tight control of reproducibility. The transition kinetics, thermal, mechanical, and biodegradation properties of the silk films prepared at different temperatures were investigated and compared by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), uniaxial tensile studies, and enzymatic degradation studies. The results revealed that this new physical processing method accurately controls structure, in turn providing control of mechanical properties, thermal stability, enzyme degradation rate, and human mesenchymal stem cell interactions. The mechanistic basis for the control is through the temperature-controlled regulation of water vapor to control crystallization. Control of silk structure via TCWVA represents a significant improvement in the fabrication of silk-based biomaterials, where control of structure-property relationships is key to regulating material properties. This new approach to control crystallization also provides an entirely new green approach, avoiding common methods that use organic solvents (methanol, ethanol) or organic acids. The method described here for silk proteins would also be universal for many other structural proteins (and likely other biopolymers), where water controls chain interactions related to material properties.
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Citations
Cites
Dynamic protein–water relationships during β-sheet formation
Hu, X., Kaplan, D., & Cebe, P. (2008) · Macromolecules · doi:10.1021/ma071551d · Cited by 315
Self-citationRelationships between degradability of silk scaffolds and osteogenesis
Park, S.-H., Gil, E. S., Shi, H., Kim, H. J., Lee, K., & Kaplan, D. L. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.04.028 · Cited by 196
Self-citation
Cited by
Silk fibroin biomaterials for tissue regenerations
Kundu, B., Rajkhowa, R., Kundu, S. C., & Wang, X. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.09.043 · Cited by 1,391
A Physically Transient Form of Silicon Electronics
Hwang, S.-W., Tao, H., Kim, D.-H., Cheng, H., Song, J.-K., Rill, E., Brenckle, M. A., Panilaitis, B., Won, S. M., Kim, Y.-S., et al. (2012) · Science · doi:10.1126/science.1226325 · Cited by 1,286
Self-citationStructures, mechanical properties and applications of silk fibroin materials
Koh, L.-D., Cheng, Y., Teng, C.-P., Khin, Y.-W., Loh, X.-J., Tee, S.-Y., Low, M., Ye, E., Yu, H.-D., Zhang, Y.-W., et al. (2015) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2015.02.001 · Cited by 1,241
Tien, L. W., Wu, F., Tang‐Schomer, M. D., Yoon, E., Omenetto, F. G., & Kaplan, D. L. (2013) · Advanced Functional Materials · doi:10.1002/adfm.201203716 · Cited by 136
Self-citationControlled release of cytokines using silk-biomaterials for macrophage polarization
Reeves, A. R. D., Spiller, K. L., Freytes, D. O., Vunjak-Novakovic, G., & Kaplan, D. L. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.09.027 · Cited by 131
Self-citationChemical, thermal, time, and enzymatic stability of silk materials with silk I structure
Zhao, M., Qi, Z., Tao, X., Newkirk, C., Hu, X., & Lu, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22084136 · Cited by 130
Self-citationScalable batch fabrication of ultrathin flexible neural probes using a bioresorbable silk layer
Cointe, C., Laborde, A., Nowak, L. G., Arvanitis, D. N., Bourrier, D., Bergaud, C., & Maziz, A. (2022) · Microsystems & Nanoengineering · doi:10.1038/s41378-022-00353-7 · Cited by 78
Film‐Based Implants for Supporting Neuron–Electrode Integrated Interfaces for The Brain
Tang‐Schomer, M. D., Hu, X., Hronik‐Tupaj, M., Tien, L. W., Whalen, M. J., Omenetto, F. G., & Kaplan, D. L. (2014) · Advanced Functional Materials · doi:10.1002/adfm.201303196 · Cited by 71
Self-citationA comprehensive review on silk fibroin as a persuasive biomaterial for bone tissue engineering
Li, M., You, J., Qin, Q., Liu, M., Yang, Y., Jia, K., Zhang, Y., & Zhou, Y. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032660 · Cited by 64
Deininger, C., Wagner, A., Heimel, P., Salzer, E., Vila, X. M., Weißenbacher, N., Grillari, J., Redl, H., Wichlas, F., Freude, T., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms23010283 · Cited by 23
Ghosh, S., Shajahan, F., Adhikari, J., Bera, A. K., Ghosh, A., & Pati, F. (2025) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.4c22349 · Cited by 13
Koo, Y., Kim, W., Hwangbo, H., Ryu, D., & Kim, G. (2026) · Advanced Functional Materials · doi:10.1002/adfm.202503933 · Cited by 8
Li, W., Shi, X., Zhang, D., Hu, J., Zhao, S., Ye, S., Wang, J., Liu, X., Zhang, Q., Wang, Z., et al. (2025) · Journal of Materials Chemistry B · doi:10.1039/d5tb00275c · Cited by 5
Metadata
- Authors
- Hu, X., Shmelev, K., Sun, L., Gil, E.-S., Park, S.-H., Cebe, P., & Kaplan, D. L.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-03-22
- Volume
- 12
- Issue
- 5
- Pages
- 1686-1696
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2007487866
- Cited by
- 649
- References
- 2
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- green
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