Rapid Determination of Polyphenols and Vitamin C in Plant-Derived Products
Abstract
Polyphenols, widely spread in our diet by the consumption of plant food products, are commonly determined using Folin-Ciocalteu reagent that interacts with other different reducing nonphenolic substances and leads to an overestimation of polyphenol content. In this paper we report an optimized Folin-Ciocalteu method to specifically determine the contents of total polyphenols and vitamin C. After the optimal conditions for the colorimetric assay were set, solid-phase extraction (Oasis HLB (hydrophilic-lipophilic balance)) was carried out to eliminate the water-soluble reducing interferences including vitamin C. Colorimetric correction was thus performed by subtracting interfering substances contained in the water washing extract from the raw extract. Moreover, vitamin C present in the water washing extract can be destroyed by heating and thus colorimetrically deduced. This procedure was set up with synthetic solutions and validated on different extracts from fruit products.
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Spectrophotometric assays are non-specific and subject to high rates of interference by vitamins, amino acids, and sugars.
“However, spectrophotometric assays are non-specific and subject to high rates of interference by vitamins, amino acids, and sugars.32”
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- Authors
- Georgé, S., Brat, P., Alter, P., & Amiot, M. J.
- Affiliations
- Centre Technique de la Conservation et des Produits Agricoles (CTCPA), Site Agroparc, F-84911 Avignon Cedex 9, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département FLHOR, TA50/16, F-34398 Montpellier Cedex 5, and Human Nutrition and Lipids, Faculté de Médecine, UMR U-476 INSERM (National Institute of Health and Medical Research)/U-1260 INRA (National Institute for Agronomic Research), 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-01-28
- Volume
- 53
- Issue
- 5
- Pages
- 1370-1373
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1983079790
- Cited by
- 804
- References
- 15
- Citations by year
- 2026: 32 · 2025: 44 · 2024: 50 · 2023: 53 · 2022: 47 · 2021: 42 · 2020: 51 · 2019: 61 · 2018: 50 · 2017: 33 · 2016: 36 · 2015: 38 · 2014: 38 · 2013: 45 · 2012: 30
- FWCI
- 11.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Agritrop (Cirad) (submittedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- polyphenols, vitamin C, Folin-Ciocalteu assay, solid-phase extraction, Oasis HLB, plant products, fruit products
- MeSH terms
- Ascorbic Acid, Beverages, Colorimetry, Flavonoids, Food Analysis, Molybdenum, Phenols, Plants, Edible, Tungsten Compounds, Polyphenols
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