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Cited by 181Polymer Surface Interaction StudiesBone Tissue Engineering MaterialsWound Healing and Treatments

In vivo leukocyte cytokine mRNA responses to biomaterials are dependent on surface chemistry

Brodbeck, William G., Voskerician, Gabriela, Ziats, Nicholas P., Nakayama, Yasuhide, Matsuda, Takehisa, Anderson, James M.·2003·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.10425
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Abstract

An in vivo mouse cage implant system was used to determine whether leukocyte cytokine mRNA responses to implanted biomaterials were dependent on surface chemistry. Surfaces displaying various chemistries (hydrophobic, hydrophilic, anionic, and cationic) were placed into stainless steel cages and implanted subcutaneously. Semiquantitative RT‐PCR analyses revealed that hydrophilic surfaces showed a decreased expression of proinflammatory cytokines, IL‐6 and IL‐8, and pro‐wound healing cytokines, IL‐10 and TGF‐β by adherent cells, and mRNA levels of the proinflammatory cytokine, IL‐1β, and the pro‐wound healing cytokine IL‐13 were decreased in surrounding, exudate cells. Cytokine responses by adherent and exudate cells to hydrophobic, anionic and cationic surfaces were similar and indicative of a strong inflammatory response at the earliest time point followed by a wound healing response at later time points. However, no differences in the types or levels of exudate cells for any of the surfaces or the empty cage at each of the respective time points were observed, indicating their respective biocompatibility. These studies identify hydrophilic surface chemistries as having significant effects on leukocyte cytokine responses in vivo by decreasing the expression of inflammatory and wound healing cytokines by inflammatory cells adherent to the biomaterial as well as present in the surrounding exudate. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 320–329, 2003

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  • Cytokine expression of biomaterial adherent macrophages in vivo was dependent on surface chemistry.

    “Cytokine expression of biomaterial adherent macrophages in vivo was also found to be dependent on surface chemistry.[120]”

    From Foreign body reaction to biomaterials (2008) · cites this paper as [120]

  • MΦ activation depends on biomaterial architecture in addition to previously implicated biomaterial chemistry and bioactivity.

    “Here we find that MΦ activation depends on biomaterial architecture in addition to previously implicated biomaterial chemistry and bioactivity (34–36).”

    From Proangiogenic scaffolds as functional templates for cardiac tissue engineering (2010) · cites this paper as [36]

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Authors
Brodbeck, W. G., Voskerician, G., Ziats, N. P., Nakayama, Y., Matsuda, T., & Anderson, J. M.
Affiliations
  • Case Western Reserve University (US)
  • National Cerebral and Cardiovascular Center (JP)
  • Kyushu University (JP)
Corresponding author
Anderson, James M.
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2002-12-31
Volume
64A
Issue
2
Pages
320-329
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.10425
OpenAlex
W2101726477
Cited by
181
References
27
Citations by year
2026: 3 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 6 · 2019: 8 · 2018: 7 · 2017: 7 · 2016: 6 · 2015: 10 · 2014: 12 · 2013: 10 · 2012: 12
FWCI
3.31
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Heart, Lung, and Blood Institute (HL55714, R01HL055714, HL 33849)
  • National Institute of General Medical Sciences (GM-20380, F32GM020380)
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
Keywords
hydrophilic surfaces, hydrophobic surfaces, anionic surfaces, cationic surfaces, inflammatory cytokines, interleukin-6, interleukin-8, IL-10, TGF-beta, IL-1β, IL-13, subcutaneous implantation, biocompatibility, adherent cells
MeSH terms
Animals, Biocompatible Materials, Cell Adhesion, Cell Fusion, Exudates and Transudates, Leukocytes, Materials Testing, RNA, Messenger, Surface Properties, Cytokines, Reverse Transcriptase Polymerase Chain Reaction, Mice

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