Cited by 3,445Metabolomics and Mass Spectrometry StudiesMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesis

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Metabolomics—the link between genotypes and phenotypes

Fiehn, Oliver·2002·Plant Molecular Biology

DOI 10.1023/a:1013713905833

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Abstract

Metabolites are the end products of cellular regulatory processes, and their levels can be regarded as the ultimate response of biological systems to genetic or environmental changes. In parallel to the terms 'transcriptome' and proteome', the set of metabolites synthesized by a biological system constitute its 'metabolome'. Yet, unlike other functional genomics approaches, the unbiased simultaneous identification and quantification of plant metabolomes has been largely neglected. Until recently, most analyses were restricted to profiling selected classes of compounds, or to fingerprinting metabolic changes without sufficient analytical resolution to determine metabolite levels and identities individually. As a prerequisite for metabolomic analysis, careful consideration of the methods employed for tissue extraction, sample preparation, data acquisition, and data mining must be taken. In this review, the differences among metabolite target analysis, metabolite profiling, and metabolic fingerprinting are clarified, and terms are defined. Current approaches are examined, and potential applications are summarized with a special emphasis on data mining and mathematical modelling of metabolism.

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Authors: Fiehn, O.
Affiliations: Max Planck Institute of Molecular Plant Physiology (DE)
Corresponding author: Fiehn, Oliver
ORCID: Fiehn, Oliver
Venue: Plant Molecular Biology
Publisher: Springer Science and Business Media LLC
Published: 2002-01
Volume: 48
Issue: 1-2
Pages: 155-171
ISSN: 0167-4412 (ISSN-L)
1573-5028
DOI: 10.1023/a:1013713905833
OpenAlex: W4294326454
Cited by: 3,445
References: 110
Citations by year: 2026: 153 · 2025: 135 · 2024: 174 · 2023: 151 · 2022: 194 · 2021: 181 · 2020: 201 · 2019: 191 · 2018: 176 · 2017: 178 · 2016: 195 · 2015: 157 · 2014: 157 · 2013: 173 · 2012: 131
FWCI: 23.53
Type: journal article
Language: en
Open access: closed
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Plant Molecular Biology (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords: plant metabolomics, metabolome, genotype-phenotype correlation, metabolite profiling, metabolic fingerprinting, plant tissue extraction, sample preparation, data mining, plant metabolism

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Metabolomics—the link between genotypes and phenotypes

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